Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,634,918	T→C	F26F (TTT→TTC)	yhiM →	inner membrane protein with a role in acid resistance

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,634,918	0	T	C	100.0%	73.9 / NA	22	F26F (TTT→TTC)	yhiM	inner membrane protein with a role in acid resistance
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (15/7);  total (15/7)

AGTGAACATATATATCGGGTGGCTTTTCAAATTAATCCCTTTGATTATGGGCTTAATTTGTATCGCGTTAGGTGGCTTTGTGCTGGAAAGTTCAGGGCAAAGCGAGTATTTCGTCGCGGGTCATGTGCTGATTTCTCTGGCGGCCATATGCCT  >  NC_000913/3634840‑3634992                                                                               |                                                                           aGTGAACATATATATCGGGTGGCTTTTCAAATTAATCCCTTTGATTATGGGCTTAATTTGTATCGCGTTAGGTGGCTTcgt                                                                          <  2:638259/81‑1 (MQ=255)     aaCATATATATCGGGTGGCTTTTCAAATTAATCCCTTTGATTATGGGCTTAATTTGTATCGCGTTAGGTGGCTTCGTGCt                                                                       <  1:638261/80‑1 (MQ=255)          atatatCGGGTGGCTTTTCAAATTAATCCCTTTGATTATGGGCTTAATTTGTATCGCGTTAGGTGGCTTCGTGCTGGAAAg                                                                 >  2:638296/1‑81 (MQ=255)          atatatCGGGTGGCTTTTCAAATTAATCCCTTTGATTATGGGCTTAATTTGTATCGCGTTAGGTGGCTTCGTGCTGGAAAg                                                                 >  1:638303/1‑81 (MQ=255)                        ttttCAAATTAATCCCTTTGATTATGGGCTTAATTTGTATCGCGTTAGGTGGCTTCGTGCTGGAAAGTTCAGGGCAAAGc                                                    >  2:638295/1‑80 (MQ=255)                            cAAATTAATCCCTTTGATTATGGGCTTAATTTGTATCGCGTTAGGTGGCTTCGTGCTGGAAAGTTCAGGGCAAAGCGAGTa                                               >  2:638303/1‑81 (MQ=255)                             aaaTTAATCCCTTTGATTATGGGCTTAATTTGTATCGCGTTAGGTGGCTTCGTGCTGGAAAGTTCAGGGCAAAGCGAGTAt                                              >  1:638290/1‑81 (MQ=255)                              aaTTAATCCCTTTGATTATGGGCTTAATTTGTATCGCGTTAGGTGGCTTCGTGCTGGAAAGTTCAGGGCAAAGCGAGTAtt                                             >  2:638292/1‑81 (MQ=255)                                 tAATCCCTTTGATTATGGGCTTAATTTGTATCGCGTTAGGTGGCTTCGTGCTGGAAAGTTCAGGGCAAAGCGAGTATTtcg                                          >  1:638300/1‑81 (MQ=255)                                  aaTCCCTTTGATTATGGGCTTAATTTGTATCGCGTTAGGTGGCTTCGTGCTGGAAAGTTCAGGGCAAAGCGAGTATTtcgt                                         >  1:638302/1‑81 (MQ=255)                                           gATTATGGGCTTAATTTGTATCGCGTTAGGTGGCTTCGTGCTGGAAAGTTCAGGGCAAAGCGAGTATTTCGTCGCGGGTCa                                >  1:638295/1‑81 (MQ=255)                                           gATTATGGGCTTAATTTGTATCGCGTTAGGTGGCTTCGTGCTGGAAAGTTCAGGGCAAAGCGAGTATTTCGTCGCGGGTCa                                >  1:638306/1‑81 (MQ=255)                                            aTTATGGGCTTAATTTGTATCGCGTTAGGTGGCTTCGTGCTGGAAAGTTCAGGGCAAAGCGAGTATTTCGTCGCGGGTCa                                <  2:638261/80‑1 (MQ=255)                                              tATGGGCTTAATTTGTATCGCGTTAGGTGGCTTCGTGCTGGAAAGTTCAGGGCAAAGCGAGTATTTCGTCGCGGGTCAtgt                             >  2:638293/1‑81 (MQ=255)                                                tGGGCTTAATTTGTATCGCGTTAGGTGGCTTCGTGCTGGAAAGTTCAGGGCAAAGCGAGTATTTCGTCGCGGGTCATGTGc                           >  2:638311/1‑81 (MQ=255)                                                        aTTTGTATCGCGTTAGGTGGCTTCGTGCTGGAAAGTTCAGGGCAAAGCGAGTATTTCGTCGCGGGTCATGTGCTGATTtct                   >  1:638301/1‑81 (MQ=255)                                                            gTATCGCGTTAGGTGGCTTCGTGCTGGAAAGTTCAGGGCAAAGCGAGTATTTCGTCGCGGGTCATGTGCTGATTTCTCTgg               >  2:638307/1‑81 (MQ=255)                                                              aTCGCGTTAGGTGGCTTCGTGCTGGAAAGTTCAGGGCAAAGCGAGTATTTCGTCGCGGGTCATGTGCTGATTTCTCTggcg             >  2:638291/1‑81 (MQ=255)                                                                 gcgTTAGGTGGCTTCGTGCTGGAAAGTTCAGGGCAAAGCGAGTATTTCGTCGCGGGTCATGTGCTGATTTCTCTGGCGGcc          <  1:638263/81‑1 (MQ=255)                                                                   gTTAGGTGGCTTCGTGCTGGAAAGTTCAGGGCAAAGCGAGTATTTCGTCGCGGGTCATGTGCTGATTTCTCTGGCGGCCat        <  2:638263/81‑1 (MQ=255)                                                                    ttAGGTGGCTTCGTGCTGGAAAGTTCAGGGCAAAGCGAGTATTTCGTCGCGGGTCATGTGCTGATTTCTCTGGCGGCCata       <  2:638264/81‑1 (MQ=255)                                                                      aGGTGGCTTCGTGCTGGAAAGTTCAGGGCAAAGCGAGTATTTCGTCGCGGGTCATGTGCTGATTTCTCTGGCGGCCatat      <  2:638265/80‑1 (MQ=255)                                                                         tGGCTTCGTGCTGGAAAGTTCAGGGCAAAGCGAGTATTTCGTCGCGGGTCATGTGCTGATTTCTCTGGCGGCCATATGCCt  <  1:638267/81‑1 (MQ=255)                                                                               |                                                                           AGTGAACATATATATCGGGTGGCTTTTCAAATTAATCCCTTTGATTATGGGCTTAATTTGTATCGCGTTAGGTGGCTTTGTGCTGGAAAGTTCAGGGCAAAGCGAGTATTTCGTCGCGGGTCATGTGCTGATTTCTCTGGCGGCCATATGCCT  >  NC_000913/3634840‑3634992

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑