Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,674,725	G→T	intergenic (+350/‑61)	yhjD → / → yhjE	putative transporter YhjD/putative transporter YhjE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,674,725	0	G	T	100.0%	88.0 / NA	25	intergenic (+350/‑61)	yhjD/yhjE	putative transporter YhjD/putative transporter YhjE
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (15/10);  total (15/10)

GAAGAAAAATCACTGGCCTAAACATTATCCCCTTTTTGCCTGATTTTTGACCATTTCCGCGATTTGTTACACATTGAAATATCACTTTTGCTGTGCGTAATATGGCTATTCGTTAGCCAAAAAATAAGAAAAGATTATGCAAGCAACAG  >  NC_000913/3674650‑3674798                                                                            |                                                                          gaagaaAAATCACTGGCCTAAACATTATCCCCTTTTTGCCTGATTTTTGACCATTTCCGCGATTTGTTACACATTTAAatg                                                                      <  2:647567/81‑2 (MQ=255)   agaaAAATCACTGGCCTAAACA‑TATCCCCTTTTTGCCTGATTTTTGACCATTTCCGCGATTTGTTACACATTTAAATATCa                                                                   >  1:647618/1‑81 (MQ=255)       aaaTCACTGGCCTAAACATTATCCCCTTTTTGCCTGATTTTTGACCATTTCCGCGATTTGTTACACATTTAAATATCACtt                                                                >  1:647592/1‑81 (MQ=255)         aTCACTGGCCTAAACATTATCCCCTTTTTGCCTGATTTTTGACCATTTCCGCGATTTGTTACACATTTAAATATTACtttt                                                              >  1:647599/1‑81 (MQ=255)               ggCCTAAACATTATCCCCTTTTTGCCTGATTTTTGACCATTTCCGCGATTTGTTACACATTTAAATATCACTTTTGCtgtt                                                        >  2:647603/1‑80 (MQ=255)                gCCTAAACATTATCCCCTTTTTGCCTGATTTTTGACCATTTCCGCGATTTGTTACACATTTAAATATCACTTTTGCtgt                                                         <  1:647569/79‑1 (MQ=255)                        aTTATCCCCTTTTTGCCTGATTTTTGACCATTTCCGCGATTTGTTACACATTTAAATATCACTTTTGCTGTGCGTAATATg                                               >  1:647598/1‑81 (MQ=255)                             ccccTTTTTGCCTGATTTTTGACCATTTCCGCGATTTGTTACACATTTAAATATCACTTTTGCTGTGCGTAATATGGCTAt                                          >  2:647602/1‑81 (MQ=255)                               ccTTTTTGCCTGATTTTTGACCATTTCCGCGATTTGTTACACATTTAAATATCACTTTTGCTGTGCGTAATATGGCTATTc                                        <  2:647569/81‑1 (MQ=255)                                   tttGCCTGATTTTTGACCATTTCCGCGATTTGTTACACATTTAAATATCACTTTTGCTGTGCGTAATATGGCTATTCGtt                                     <  1:647571/80‑1 (MQ=255)                                       ccTGATTTTTGACCATTTCCGCGATTTGTTACACATTTAAATATCACTTTTGCTGTGCGTAATATGGCTATTCGTTAGcc                                 >  1:647605/1‑80 (MQ=255)                                       ccTGATTTTTGACCATTTCCGCGATTTGTTACACATTTAAATATCACTTTTGCTGTGCGTAATATGGCTATTCGTTAGcc                                 <  2:647571/80‑1 (MQ=255)                                         tGATTTTTGACCATTTCCGCGATTTGTTACACATTTAAATATCACTTTTGCTGTGCGTAATATGGCTATTCGTTAGCCaa                               >  1:647597/1‑80 (MQ=255)                                          gATTTTTGACCATTTCCGCGATTTGTTACACATTTAAATATCACTTTTGCTGTGCGTAATATGGCTATTCGTTAGCCaaa                              >  2:647608/1‑80 (MQ=255)                                              tttGACCATTTCCGCGATTTGTTACACATTTAAATATCACTTTTGCTGTGCGTAATATGGCTATTCGTTAGCCAAAAAAt                          <  2:647572/80‑1 (MQ=255)                                                 gACCATTTCCGCGATTTGTTACACATTTAAATATCACTTTTGCTGTGCGTAATATGGCTATTCGTTAGCCAAAAAATAAg                       <  2:647573/80‑1 (MQ=255)                                                    cATTTCCGCGATTTGTTACACATTTAAATATCACTTTTGCTGTGCGTAATATGGCTATTCGTTAGCCAAAAAATAAGaa                     >  1:647608/1‑79 (MQ=255)                                                         ccGCGATTTGTTACACATTTAAATATCACTTTTGCTGTGCGTAATATGGCTATTCGTTAGCCAAAAAATAAGAAAAGATTa              >  2:647609/1‑81 (MQ=255)                                                           gcgATTTGTTACACATTTAAATATCACTTTTGCTGTGCGTAATATGGCTATTCGTTAGCCAAAAAATAAGAAAAGATTATg            <  2:647574/81‑1 (MQ=255)                                                            cgATTTGTTACACATTTAAATATCACTTTTGCTGTGCGTAATATGGCTATTCGTTAGCCAAAAAATAAGAAAAGATTATg            >  1:647602/1‑80 (MQ=255)                                                             gATTTGTTACACATTTAAATATCACTTTTGCTGTGCGTAATATGGCTATTCGTTAGCCAAAAAATAAGAAAAGATTATgc           >  1:647629/1‑80 (MQ=255)                                                                   ttACACATTTAAATATCACTTTTGCTGTGCGTAATATGGCTATTCGTTAGCCAAAAAATAAGAAAAGATTATGCAAGCAAc    <  2:647575/81‑1 (MQ=255)                                                                    tACACATTTAAATATCACTTTTGCTGTGCGTAATATGGCTATTCGTTAGCCAAAAAATAAGAAAAGATTATgcaagcaa     >  1:647614/1‑79 (MQ=255)                                                                    tACACATTTAAATATCACTTTTGCTGTGCGTAATATGGCTATTCGTTAGCCAAAAAATAAGAAAAGATTATgcaagcaa     >  1:647611/1‑79 (MQ=255)                                                                      cacaTTTAAATATCACTTTTGCTGTGCGTAATATGGCTATTCGTTAGCCAAAAAATAAGAAAAGATTATGCAAGCAACAg  <  2:647576/80‑1 (MQ=255)                                                                            |                                                                          GAAGAAAAATCACTGGCCTAAACATTATCCCCTTTTTGCCTGATTTTTGACCATTTCCGCGATTTGTTACACATTGAAATATCACTTTTGCTGTGCGTAATATGGCTATTCGTTAGCCAAAAAATAAGAAAAGATTATGCAAGCAACAG  >  NC_000913/3674650‑3674798

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑