Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,708,650	T→C	noncoding (43/77 nt)	proK ←	tRNA‑Pro

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,708,650	0	T	C	100.0%	118.5 / NA	34	noncoding (43/77 nt)	proK	tRNA‑Pro
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (18/16);  total (18/16)

CAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCTTCGTCCCGAACGAAGTGCGCTACCAGGCTGCGCCAATCACCGAATGCGGGGCGCATCTTACTGCGCAGATACGCCC  >  NC_000913/3708574‑3708726                                                                             |                                                                             cAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCCTc                                                                            <  1:656917/79‑1 (MQ=255) cAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCCTc                                                                            <  1:656916/79‑1 (MQ=255)  aGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCCTCGTc                                                                         <  1:656918/81‑1 (MQ=255)  aGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCCTCGTc                                                                         <  1:656919/81‑1 (MQ=255)   gATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCCTCGTcc                                                                        <  1:656920/81‑1 (MQ=255)   gATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCCTCGTcc                                                                        >  1:656964/1‑81 (MQ=255)        aaaaaaGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCCTCGTCCcgaac                                                                   <  1:656921/81‑1 (MQ=255)               ccTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCCTCGTCCCGAACGAAGTgc                                                            <  2:656921/81‑1 (MQ=255)                     cGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCCTCGTCCCGAACGAAGTGCGCTAc                                                       >  1:656969/1‑80 (MQ=255)                      gTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCCTCGTCCCGAACGAAGTGCGCTAc                                                       >  2:656967/1‑79 (MQ=255)                        tGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCCTCGTCCCGAACGAAGTGCGCTACCAgg                                                   >  2:656964/1‑81 (MQ=255)                          aGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCCTCGTCCCGAACGAAGTGCGCTACCAGGc                                                  <  1:656923/80‑1 (MQ=255)                          aGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCCTCGTCCCGAACGAAGTGCGCTACCAGGCt                                                 >  1:656966/1‑81 (MQ=255)                             aGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCCTCGTCCCGAACGAAGTGCGCTACCAGGCTgcg                                              >  2:656978/1‑81 (MQ=255)                             aGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCCTCGTCCCGAACGAAGTGCGCTACCAGGCTgcg                                              <  1:656924/81‑1 (MQ=255)                                  tttCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCCTCGTCCCGAACGAAGTGCGCTACCAGGCTGCGCCAAt                                         >  1:656974/1‑81 (MQ=255)                                  tttCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCCTCGTCCCGAACGAAGTGCGCTACCAGGCTGCGCCAAt                                         >  1:656977/1‑81 (MQ=255)                                      gAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCCTCGTCCCGAACGAAGTGCGCTACCAGGCTGCGCCAATCAcc                                     >  1:656981/1‑81 (MQ=255)                                      gAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCCTCGTCCCGAACGAAGTGCGCTACCAGGCTGCGCCAATCAcc                                     >  2:656993/1‑81 (MQ=255)                                       aaTTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCCTCGTCCCGAACGAAGTGCGCTACCAGGCTGCGCCAATCAcc                                     >  1:656984/1‑80 (MQ=255)                                         tttGGTCGGTGATAGAGGATTCGAACCTCCGACCCCCTCGTCCCGAACGAAGTGCGCTACCAGGCTGCGCCAATCACCGaa                                  <  1:656925/81‑1 (MQ=255)                                            ggTCGGTGATAGAGGATTCGAACCTCCGACCCCCTCGTCCCGAACGAAGTGCGCTACCAGGCTGCGCCAATCACCGAATGc                               >  2:656981/1‑81 (MQ=255)                                                  tGATAGAGGATTCGAACCTCCGACCCCCTCGTCCCGAACGAAGTGCGCTACCAGGCTGCGCCAATCACCGAATGCGGGgcg                         <  2:656925/81‑1 (MQ=255)                                                   gATAGAGGATTCGAACCTCCGACCCCCTCGTCCCGAACGAAGTGCGCTACCAGGCTGCGCCAATCACCGAATGCGGGgcg                         >  1:656990/1‑80 (MQ=255)                                                       gagGATTCGAACCTCCGACCCCCTCGTCCCGAACGAAGTGCGCTACCAGGCTGCGCCAATCACCGAATGCGGGGCGCATc                     <  1:656927/80‑1 (MQ=255)                                                         ggATTCGAACCTCCGACCCCCTCGTCCCGAACGAAGTGCGCTACCAGGCTGCGCCAATCACCGAATGCGGGGCGCATCTTa                  >  2:656982/1‑81 (MQ=255)                                                           aTTCGAACCTCCGACCCCCTCGTCCCGAACGAAGTGCGCTACCAGGCTGCGCCAATCACCGAATGCGGGGCGCATCTTACt                <  1:656929/81‑1 (MQ=255)                                                           aTTCGAACCTCCGACCCCCTCGTCCCGAACGAAGTGCGCTACCAGGCTGCGCCAATCACCGAATGCGGGGCGCATCTTACt                <  1:656928/81‑1 (MQ=255)                                                            ttCGAACCTCCGACCCCCTCGTCCCGAACGAAGTGCGCTACCAGGCTGCGCCAATCACCGAATGCGGGGCGCATCTTACTg               <  2:656929/81‑1 (MQ=255)                                                              cGAACCTCCGACCCCCTCGTCCCGAACGAAGTGCGCTACCAGGCTGCGCCAATCACCGAATGCGGGGCGCATCTTACTgcg             >  2:656999/1‑81 (MQ=255)                                                              cGAACCTCCGACCCCCTCGTCCCGAACGAAGTGCGCTACCAGGCTGCGCCAATCACCGAATGCGGGGCGCATCTTACTgcg             >  1:656980/1‑81 (MQ=255)                                                                      cGACCCCCTCGTCCCGAACGAAGTGCGCTACCAGGCTGCGCCAATCACCGAATGCGGGGCGCATCTTACTGCGCAGATa       <  2:656930/79‑1 (MQ=255)                                                                      cGACCCCCTCGTCCCGAACGAAGTGCGCTACCAGGCTGCGCCAATCACCGAATGCGGGGCGCATCTTACTGCGCAGATACg     >  2:656974/1‑81 (MQ=255)                                                                         ccccCTCGTCCCGAACGAAGTGCGCTACCAGGCTGCGCCAATCACCGAATGCGGGGCGCATCTTACTGCGCAGATACGccc  >  2:656977/1‑81 (MQ=255)                                                                             |                                                                             CAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCTTCGTCCCGAACGAAGTGCGCTACCAGGCTGCGCCAATCACCGAATGCGGGGCGCATCTTACTGCGCAGATACGCCC  >  NC_000913/3708574‑3708726

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑