Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,737,646	G→A	T50T (ACG→ACA)	malS →	alpha‑amylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,737,646	0	G	A	100.0%	147.1 / NA	41	T50T (ACG→ACA)	malS	alpha‑amylase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (19/22);  total (19/22)

CCGCCTTTAGCGAACAGGGGACAGGAACATTTGTCAGCCACGCGCAGTTGCCCAAAGGTACGCGTCCACTAACGCTAAATTTTGACCAACAGTGCTGGCAGCCTGCGGATGCGATAAAACTCAATCAGATGCTTTCCCTGCAACC  >  NC_000913/3737573‑3737717                                                                          |                                                                        ccGCCTTTAGCGAACAGGGGACAGGAACATTTGTCAGCCACGCGCAGTTGCCCAAAGGTACGCGTCCACTAACACTAAAtt                                                                  >  2:664202/1‑81 (MQ=255)   gCCTTTAGCGAACAGGGGACAGGAACATTTGTCAGCCACGCGCAGTTGCCCAAAGGTACGCGTCCACTAACACTAAAtttt                                                                >  1:664183/1‑81 (MQ=255)    ccTTTAGCGAACAGGGGACAGGAACATTTGTCAGCCACGCGCAGTTGCCCAAAGGTACGCGTCCACTAACACTAAATTTTg                                                               <  1:664106/81‑1 (MQ=255)     cTTTAGCGAACAGGGGACAGGAACATTTGTCAGCCACGCGCAGTTGCCCAAAGGTACGCGTCCACTAACACTAAATTTTGa                                                              >  1:664190/1‑81 (MQ=255)      tttAGCGAACAGGGGACAGGAACATTTGTCAGCCACGCGCAGTTGCCCAAAGGTACGCGTCCACTAACACTAAATTTTGAc                                                             >  2:664181/1‑81 (MQ=255)        tAGCGAACAGGGGACAGGAACATTTGTCAGCCACGCGCAGTTGCCCAAAGGTACGCGTCCACTAACACTAAATTTTGAcc                                                            >  2:664195/1‑80 (MQ=255)        tAGCGAACAGGGGACAGGAACATTTGTCAGCCACGCGCAGTTGCCCAAAGGTACGCGTCCACTAACACTAAATTTTGAcc                                                            >  1:664202/1‑80 (MQ=255)        tAGCGAACAGGGGACAGGAACATTTGTCAGCCACGCGCAGTTGCCCAAAGGTACGCGTCCACTAACACTAAATTTTGAc                                                             <  2:664106/79‑1 (MQ=255)         aGCGAACAGGGGACAGGAACATTTGTCAGCCACGCGCAGTTGCCCAAAGGTACGCGTCCACTAACACTAAATTTTGACCaa                                                          >  1:664174/1‑81 (MQ=255)          gCGAACAGGGGACAGGAACATTTGTCAGCCACGCGCAGTTGCCCAAAGGTACGCGTCCACTAACACTAAATTTTGACCat                                                          <  2:664107/80‑2 (MQ=255)            gAACAGGGGACAGGAACATTTGTCAGCCACGCGCAGTTGCCCAAAGGTACGCGTCCACTAACACTAAATTTTGACCAACAg                                                       >  1:664180/1‑81 (MQ=255)             aaCAGGGGACAGGAACATTTGTCAGCCACGCGCAGTTGCCCAAAGGTACGCGTCCACTAACACTAAATTTTGACCAACAGt                                                      >  1:664191/1‑81 (MQ=255)                aGGGGACAGGAACATTTGTCAGCCACGCGCAGTTGCCCAAAGGTACGCGTCCACTAACACTAAATTTTGACCAACAGTGCt                                                   <  2:664108/81‑1 (MQ=255)                   ggACAGGAACATTTGTCAGCCACGCGCAGTTGCCCAAAGGTACGCGTCCACTAACACTAAATTTTGACCAACAGTGCTGGc                                                <  1:664110/81‑1 (MQ=255)                    gACAGGAACATTTGTCAGCCACGCGCAGTTGCCCAAAGGTACGCGTCCACTAACACTAAATTTTGACCAACAGTGCTgg                                                 >  2:664209/1‑79 (MQ=255)                    gACAGGAACATTTGTCAGCCACGCGCAGTTGCCAAAAGGTACGCGTCCACTAACACTAAATTTTGACCAACAGTGCTGGCa                                               <  1:664111/81‑1 (MQ=255)                      cAGGAACATTTGTCAGCCACGCGCAGTTGCCCAAAGGTACGCGTCCACTAACACTAAATTTTGACCAACAGTGCTGGCAGc                                             >  1:664187/1‑81 (MQ=255)                       aGGAACATTTGTCAGCCACGCGCAGTTGCCCAAAGGTACGCGTCCACTAACACTAAATTTTGACCAACAGTGCTGGCAGcc                                            >  1:664175/1‑81 (MQ=255)                       aGGAACATTTGTCAGCCACGCGCAGTTGCCCAAAGGTACGCGTCCACTAACACTAAATTTTGACCAACAGTGCTGGCAGcc                                            <  1:664112/81‑1 (MQ=255)                         gAACATTTGTCAGCCACGCGCAGTTGCCCAAAGGTACGCGTCCACTAACACTAAATTTTGACCAACAGTGCTGGCAGCCTg                                          <  2:664112/81‑1 (MQ=255)                          aaCATTTGTCAGCCACGCGCAGTTGCCCAAAGGTACGCGTCCACTAACACTAAATTTTGACCAACAGTGCTGGCAGCCTGc                                         >  1:664189/1‑81 (MQ=255)                            cATTTGTCAGCCACGCGCAGTTGCCCAAAGGTACGCGTCCACTAACACTAAATTTTGACCAACAGTGCTGGCAGCCTGCgg                                       <  2:664113/81‑1 (MQ=255)                               ttGTCAGCCACGCGCAGTTGCCCAAAGGTACGCGTCCACTAACACTAAATTTTGACCAACAGTGCTGGCAGCCTGCGGATg                                    >  1:664193/1‑81 (MQ=255)                                 gTCAGCCACGCGCAGTTGCCCAAAGGTACGCGTCCACTAACACTAAATTTTGACCAACAGTGCTGGCAGCCTGCGGATGc                                   <  2:664114/80‑1 (MQ=255)                                  tCAGCCACGCGCAGTTGCCCAAAGGTACGCGTCCACTAACACTAAATTTTGACCAACAGTGCTGGCAGCCTGCGGATGCg                                  <  2:664115/80‑1 (MQ=255)                                  tCAGCCACGCGCAGTTGCCCAAAGGTACGCGTCCACTAACACTAAATTTTGACCAACAGTGCTGGCAGCCTGCGGATGCg                                  <  1:664117/80‑1 (MQ=255)                                  tCAGCCACGCGCAGTTGCCCAAAGGTACGCGTCCACTAACACTAAATTTTGACCAACAGTGCTGGCAGCCTGCGGATGCg                                  <  1:664119/80‑1 (MQ=255)                                  tCAGCCACGCGCAGTTGCCCAAAGGTACGCGTCCACTAACACTAAATTTTGACCAACAGTGCTGGCAGCCTGCGGATGCGa                                 <  1:664118/81‑1 (MQ=255)                                    aGCCACGCGCAGTTGCCCAAAGGTACGCGTCCACTAACACTAAATTTTGACCAACAGTGCTGGCAGCCTGCGGATGCGAt                                <  2:664119/80‑1 (MQ=255)                                      ccACGCGAAGTTGACAAAAGGTACGAGTCAAATAACACTAAATTTTGACAAACAGTGCTGGCAGCCTGCGGATGCGATaaa                             <  1:664121/81‑1 (MQ=255)                                         cgcgCAGTTGCCCAAAGGTACGCGTCCACTAACACTAAATTTTGACCAACAGTGCTGGCAGCCTGCGGATGCGATAAAACt                          <  2:664121/81‑1 (MQ=255)                                                  gCCCAAAGGTACGCGTCCACTAACACTAAATTTTGACCAACAGTGCTGGCAGCCTGCGGATGCGATAAAACTCAATCAGAt                 >  2:664199/1‑81 (MQ=255)                                                   cccAAAGGTACGCGTCCACTAACACTAAATTTTGACCAACAGTGCTGGCAGCCTGCGGATGCGATAAAACTCAATCAGAt                 <  1:664123/80‑1 (MQ=255)                                                    ccAAAGGTACGCGTCCACTAACACTAAATTTTGACCAACAGTGCTGGCAGCCTGCGGATGCGATAAAACTCAATCAGATGc               >  2:664191/1‑81 (MQ=255)                                                       aaGGTACGCGTCCACTAACACTAAATTTTGACCAACAGTGCTGGCAGCCTGCGGATGCGATAAAACTCAATCAGATGCttt            <  2:664123/81‑1 (MQ=255)                                                           tACGCGTCCACTAACACTAAATTTTGACCAACAGTGCTGGCAGCCTGCGGATGCGATAAAACTCAATCAGATGCTTTCCCt        >  1:664186/1‑81 (MQ=255)                                                           tACGCGTCCACTAACACTAAATTTTGACCAACAGTGCTGGCAGCCTGCGGATGCGATAAAACTCAATCAGATGCTTTCCCt        <  2:664124/81‑1 (MQ=255)                                                           tACGCGTCCACTAACACTAAATTTTGACCAACAGTGCTGGCAGCCTGCGGATGCGATAAAACTCAATCAGATGCTTTCCCt        <  2:664125/81‑1 (MQ=255)                                                              gcgTCCACTAACACTAAATTTTGACCAACAGTGCTGGCAGCCTGCGGATGCGATAAAACTCAATCAGATGCTTTCCCTGc      >  2:664214/1‑80 (MQ=255)                                                                gTCCACTAACACTAAATTTTGACCAACAGTGCTGGCAGCCTGCGGATGCGATAAAACTCAATCAGATGCTTTCCCTGCAAc   >  2:664193/1‑81 (MQ=255)                                                                 tCCACTAACACTAAATTTTGACCAACTGTGCTGGCAGCCTGCGGATGCGATAAAACTCAATCAGATGCTTTCCCTGCAAcc  <  1:664127/81‑1 (MQ=255)                                                                          |                                                                        CCGCCTTTAGCGAACAGGGGACAGGAACATTTGTCAGCCACGCGCAGTTGCCCAAAGGTACGCGTCCACTAACGCTAAATTTTGACCAACAGTGCTGGCAGCCTGCGGATGCGATAAAACTCAATCAGATGCTTTCCCTGCAACC  >  NC_000913/3737573‑3737717

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑