Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,795,757	G→C	A277P (GCG→CCG)	waaF →	ADP‑heptose‑‑LPS heptosyltransferase 2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,795,757	0	G	C	100.0%	126.2 / NA	37	A277P (GCG→CCG)	waaF	ADP‑heptose‑‑LPS heptosyltransferase 2
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (15/22);  total (15/22)

GCTTGATCAAGCGGTTATCCTGATTGCAGCCTGTAAAGCCATTGTCACTAACGATTCTGGCCTGATGCATGTTGCGGCGGCGCTCAATCGTCCGCTGGTTGCCCTGTATGGTCCGAGTAGCCCGGACTTCACACCGCCGCTATCCCATAAAGC  >  NC_000913/3795681‑3795833                                                                             |                                                                             gCTTGATCAAGCGGTTATCCTGATTGCAGCCTGTAAAGCCATTGTCACTAACGATTCTGGCCTGATGCATGTTgcgccggc                                                                          >  2:679515/1‑81 (MQ=255) gCTTGATCAAGCGGTTATCCTGATTGCAGCCTGTAAAGCCATTGTCACTAACGATTCTGGCCTGATGCATGTTgcgccggc                                                                          <  2:679461/81‑1 (MQ=255) gCTTGATCAAGCGGTTATCCTGATTGCAGCCAGTAAAGCCATTGTCACTAACGATTCTGGCCTGATGCATGTTgcgccggc                                                                          <  2:679460/81‑1 (MQ=255)      aTCAAGCGGTTATCCTGATTGCAGCCTGTAAAGCCATTGTCACTAACGATTCTGGCCTGATGCATGTTGCGCCGGCGCTc                                                                      >  2:679513/1‑80 (MQ=255)      aTCAAGCGGTTATCCTGATTGCAGCCTGTAAAGCCATTGTCACTAACGATTCTGGCCTGATGCATGTTGCGCCGGCGCTc                                                                      <  1:679463/80‑1 (MQ=255)         aaGCGGTTATCCTGATTGCAGCCTGTAAAGCCATTGTCACTAACGATTCTGGCCTGATGCATGTTGCGCCGGCGCTCAATc                                                                  >  2:679518/1‑81 (MQ=255)           gCGGTTATCCTGATTGCAGCCTGTAAAGCCATTGTCACTAACGATTCTGGCCTGATGCATGTTGCGCCGGCGCTCAATCGt                                                                <  1:679464/81‑1 (MQ=255)           gCGGTTATCCTGATTGCAGCCTGTAAAGCCATTGTCACTAACGATTCTGGCCTGATGCATGTTGCGCCGGCGCTCAATCGt                                                                <  2:679464/81‑1 (MQ=255)            cGGTTATCCTGATTGCAGCCTGTAAAGCCATTGTCACTAACGATTCTGGCCTGATGCATGTTGCGCCGGCGCTCAATCGTc                                                               <  1:679466/81‑1 (MQ=255)               ttATCCTGATTGCAGCCTGTAAAGCCATTGTCACTAACGATTCTGGCCTGATGCATGTTGCGCCGGCGCTCAATCGTCCGc                                                            <  2:679466/81‑1 (MQ=255)                tATCCTGATTGCAGCCTGTAAAGCCATTGTCACTAACGATTCTGGCCTGATGCATGTTGCGCCGGCGCTCAATCGTCCGCt                                                           >  1:679536/1‑81 (MQ=255)                tATCCTGATTGCAGCCTGTAAAGCCATTGTCACTAACGATTCTGGCCTGATGCATGTTGCGCCGGCGCTCAATCGTCCGCt                                                           >  1:679531/1‑81 (MQ=255)                     tGATTGCAGCCTGTAAAACCATTGTCACCAACGATTCTTGCCTGATGCATGTTGCGCCCGCGCTCAATCGTCCGCttgttg                                                      >  2:679520/1‑81 (MQ=255)                      gATTGCAGCCTGTAAAGCCATTGTCACTAACGATTCTGGCCTGATGCATGTTGCGCCGGCGCTCAATCGTCCGCTGGTTGc                                                     >  1:679528/1‑81 (MQ=255)                                  tAAAGCCATTGTCACTAACGATTCTGGCCTGATGCATGTTGCGCCGGCGCTCAATCGTCCGCTGGTTGCCCTGTATGGTcc                                         <  1:679468/81‑1 (MQ=255)                                    aaGCCATTGTCACTAACGATTCTGGCCTGATGCATGTTGCGCCGGCGCTCAATCGTCCGCTGGTTGCCCTGTATGGTcc                                         >  1:679554/1‑79 (MQ=255)                                    aaGCCATTGTCACTAACGATTCTGGCCTGATGCATGTTGCGCCGGCGCTCAATCGTCCGCTGGTTGCCCTGTATGGTCCg                                        <  1:679470/80‑1 (MQ=255)                                    aaGCCATTGTCACTAACGATTCTGGCCTGATGCATGTTGCGCCGGCGCTCAATCGTCCGCTGGTTGCCCTGTATGGTCCGa                                       <  1:679469/81‑1 (MQ=255)                                      gCCATTGTCACTAACGATTCTGGCCTGATGCATGTTGCGCCGGCGCTCAATCGTCCGCTGGTTGCCCTGTATGGTCCGAg                                      >  1:679537/1‑80 (MQ=255)                                      gCCATTGTCACTAACGATTCTGGCCTGATGCATGTTGCGCCGGCGCTCAATCGTCCGCTGGTTGCCCTGTATGGTCCGAGt                                     <  2:679472/81‑1 (MQ=255)                                      gCCATTGTCACTAACGATTCTGGCCTGATGCATGTTGCGCCGGCGCTCAATCGTCCGCTGGTTGCCCTGTATGGTCCGAGt                                     <  2:679471/81‑1 (MQ=255)                                      gCCATTGTCACTAACGATTCTGGCCTGATGCATGTTGCGCCGGCGCTCAATCGTCCGCTGGTTGCCCTGTATGGTCCGAGt                                     <  1:679471/81‑1 (MQ=255)                                         aTTGTCACTAACGATTCTGGCCTGATGCATGTTGCGCCGGCGCTCAATCGTCCGCTGGTTGCCCTGTATGGTCCGAGTAGc                                  >  1:679523/1‑81 (MQ=255)                                           tGTCACTAACGATTCTGGCCTGATGCATGTTGCGCCGGCGCTCAATCGTCCGCTGGTTGCCCTGTATGGTCCGAGTAGccc                                >  1:679527/1‑81 (MQ=255)                                            gTCACTAACGATTCTGGCCTGATGCATGTTGCGCCGGCGCTCAATCGTCCGCTGGTTGCCCTGTATGGTCCGAGTAGCCCg                               >  1:679530/1‑81 (MQ=255)                                             tCACTAACGATTCTGGCCTGATGCATGTTGCGCCGGCGCTCAATCGTCCGCTGGTTGCCCTGTATGGTCCGAGTAGCCCg                               <  1:679474/80‑1 (MQ=255)                                               aCTAACGATTCTGGCCTGATGCATGTTGCGCCGGCGCTCAATCGTCCGCTGGTTGCCCTGTATGGTCCGAGTAGCCCGGAc                            <  1:679475/81‑1 (MQ=255)                                                  aaCGATTCTGGCCTGATGCATGTTGCGCCGGCGCTCAATCGTCCGCTGGTTGCCCTGTATGGTCCGAGTAGCCCGGACtt                          >  1:679525/1‑80 (MQ=255)                                                   aCGATTCTGGCCTGATGCATGTTGCGCCGGCGCTCAATCGTCCGCTGGTTGCCCTGTATGGTCCGAGTAGCCCGGACtt                          <  1:679477/79‑1 (MQ=255)                                                   aCGATTCTGGCCTGATGCATGTTGCGCCGGCGCTCAATCGTCCGCTGGTTGCCCTGTATGGTCCGAGTAGCCCGGACTTca                        <  1:679476/81‑1 (MQ=255)                                                    cGATTCTGGCCTGATGCATGTTGCGCCGGCGCTCAATCGTCCGCTGGTTGCCCTGTATGGTCCGAGTAGCCCGGACTTcac                       <  2:679477/81‑1 (MQ=255)                                                      aTTCTGGCCTGATGCATGTTGCGCCGGCGCTCAATCGTCCGCTGGTTGCCCTGTATGGTCCGAGTAGCCCGGACTTCacac                     <  2:679478/81‑1 (MQ=255)                                                       ttCTGGCCTGATGCATGTTGCGCCGGCGCTCAATCGTCCGCTGGTTGCCCTGTATGGTCCGAGTAGCCCGGACTTCACAcc                    <  2:679479/81‑1 (MQ=255)                                                         cTGGCCTGATGCATGTTGCGCCGGCGCTCAATCGTCCGCTGGTTGCCCTGTATGGTCCGAGTAGCCCGGACTTCACAccgc                  >  2:679534/1‑81 (MQ=255)                                                                 aTGCATGTTGCGCCGGCGCTCAATCGTCCGCTGGTTGCCCTGTATGGTCCGAGTAGCCCGGACTTCACACCGCCGCTATcc          <  1:679482/81‑1 (MQ=255)                                                                 aTGCATGTTGCGCCGGCGCTCAATCGTCCGCTGGTTGCCCTGTATGGTCCGAGTAGCCCGGACTTCACACCGCCGCTATc           <  2:679480/80‑1 (MQ=255)                                                                  tGCATGTTGCGCCGGCGCTCAATCGTCCGCTGGTTGCCCTGTATGGTCCGAGTAGCCCGGACTTCACACCGCCGCTATccc         <  2:679482/81‑1 (MQ=255)                                                                   gCATGTTGCGCCGGCGCTCAATCGTCCGCTGGTTGCCCTGTATGGTCCGAGTAGCCCGGACTTCACACCGCCGCTATCCCa        >  2:679539/1‑81 (MQ=255)                                                                      tGTTGCGCCGGCGCTCAATCGTCCGCTGGTTGCCCTGTATGGTCCGAGTAGCCCGGACTTCACACCGCCGCTATCCCATaa     <  1:679484/81‑1 (MQ=255)                                                                       gTTGCGCCGGCGCTCAATCGTCCGCTGGTTGCCCTGTATGGTCCGAGTAGCCCGGACTTCACACCGCCGCTATCCCATa      >  2:679544/1‑79 (MQ=255)                                                                          gcgccggcgCTCAATCGTCCGCTGGTTGCCCTGTATGGTCCGAGTAGCCCGGACTTCACACCGCCGCTATCCCATAAAgc  <  1:679485/80‑1 (MQ=255)                                                                             |                                                                             GCTTGATCAAGCGGTTATCCTGATTGCAGCCTGTAAAGCCATTGTCACTAACGATTCTGGCCTGATGCATGTTGCGGCGGCGCTCAATCGTCCGCTGGTTGCCCTGTATGGTCCGAGTAGCCCGGACTTCACACCGCCGCTATCCCATAAAGC  >  NC_000913/3795681‑3795833

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑