Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,826,047	A→T	I280F (ATC→TTC)	recG →	ATP‑dependent DNA helicase RecG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,826,047	0	A	T	100.0%	162.7 / NA	46	I280F (ATC→TTC)	recG	ATP‑dependent DNA helicase RecG
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (26/20);  total (26/20)

ACACGCTGAAAAATAAACTCCTCGCCGCCTTACCGTTCAAGCCAACGGGCGCACAGGCACGCGTAGTGGCGGAGATCGAGCGCGATATGGCGCTGGATGTGCCGATGATGCGTCTGGTGCAGGGCGATGTAGGTTCCGGTAAAACGCTGGTC  >  NC_000913/3825973‑3826124                                                                           |                                                                              acacGCTGAAAAATAAACTCCTCGCCGCCTTACCGTTCAAGCCAACGGGCGCACAGGCACGCGTAGTGGCGGAGTTCGAgc                                                                         <  1:686383/81‑1 (MQ=255)  cacGCTGAAAAATAAACTCCTCGCCGCCTTACCGTTCAAGCCAACGGGCGCACAGGCACGCGTAGTGGCGGAGTTCGAgcg                                                                        >  1:686453/1‑81 (MQ=255)    cGCTGAAAAATAAACTCCTCGCCGCCTTACCGTTCAAGCCAACGGGCGCACAGGCACGCGTAGTGGCGGAGTTCGAgcgc                                                                       >  1:686439/1‑80 (MQ=255)         aaaaaTAAACTCCTCGCCGCCTTACCGTTCAAGCCAACGGGCGCACAGGCACGCGTAGTGGCGGAGTTCGAGCGCGatat                                                                  <  1:686384/80‑1 (MQ=255)         aaaaaTAAACTCCTCGCCGCCTTACCGTTCAAGCCAACGGGCGCACAGGCACGCGTAGTGGCGGAGTTCGAGCGCGATATg                                                                 >  1:686441/1‑81 (MQ=255)           aaaTAAACTCCTCGCCGCCTTACCGTTCAAGCCAACGGGCGCACAGGCACGCGTAGTGGCGGAGTTCGAGCGCGATATGgc                                                               >  2:686448/1‑81 (MQ=255)            aaTAAACTCCTCGCCGCCTTACCGTTCAAGCCAACGGGCGCACAGGCACGCGTAGTGGCGGAGTTCGAGCGCGATATGgcg                                                              <  1:686385/81‑1 (MQ=255)               aaaCTCCTCGCCGCCTTACCGTTCAAGCCAACGGGCGCACAGGCACGCGTAGTGGCGGAGTTCGAGCGCGATATGGCGCTg                                                           >  2:686454/1‑81 (MQ=255)                     ctcGCCGCCTTACCGTTCAAGCCAACGGGCGCACAGGCACGCGTAGTGGCGGAGTTCGAGCGCGATATGGCGCTGGAtgtg                                                     <  1:686386/81‑1 (MQ=255)                       cgccgcCTTACCGTTCAAGCCAACGGGCGCACAGGCACGCGTAGTGGCGGAGTTCGAGCGCGATATGGCGCTGGATGTGcc                                                   >  2:686465/1‑81 (MQ=255)                        gccgccTTACCGTTCAAGCCAACGGGCGCACAGGCACGCGTAGTGGCGGAGTTCGAGCGCGATATGGCGCTGGATGTGcc                                                   >  2:686451/1‑80 (MQ=255)                           gccTTACCGTTCAAGCCGACGGGCGCACAGGCACGCGTAGTGGCGGAGTTCGAGCGCGATATGGCGCTGGATGTGCCgatg                                               <  1:686387/81‑1 (MQ=255)                           gccTTACCGTTCAAGCCAACGGGCGCACAGGCACGCGTAGTGGCGGAGTTCGAGCGCGATATGGCGCTGGATGTGCCgat                                                >  2:686449/1‑80 (MQ=255)                                aCCGTTCAAGCCAACGGGCGCACAGGCACGCGTAGTGGCGGAGTTCTAGCGCGATATGGCGCTGGATGTGCCGATGATGCg                                          >  1:686454/1‑81 (MQ=255)                                 ccGTTCAAGCCAACGGGCGCACAGGCACGCGTAGTGGCGGAGTTCGAGCGCGATATGGCGCTGGATGTGCCGATGATGCGt                                         >  1:686457/1‑81 (MQ=255)                                  cGTTCAAGCCAACGGGCGCACAGGCACGCGTAGTGGCGGAGTTCGAGCGCGATATGGCGCTGGATGTGCCGATGATGCGTc                                        >  2:686447/1‑81 (MQ=255)                                    ttCAGGCCAACGGGCGCACAGGCACGCGTAGTGGCGGAGTTCGAGCGCGATATGGCGCTGGATGTGCCGATGATGCGTCTg                                      <  2:686387/81‑1 (MQ=255)                                       aaGCCAACGGGCGCACAGGCACGCGTAGTGGCGGAGTTCGAGCGCGATATGGCGCTGGATGTGCCGATGATGCGTCTGGt                                    <  2:686390/80‑1 (MQ=255)                                       aaGCCAACGGGCGCACAGGCACGCGTAGTGGCGGAGTTCGAGCGCGATATGGCGCTGGATGTGCCGATGATGCGTCTGGTg                                   >  1:686423/1‑81 (MQ=255)                                       aaGCCAACGGGCGCACAGGCACGCGTAGTGGCGGAGTTCGAGCGCGATATGGCGCTGGATGTGCCGATGATGCGTCTGGTg                                   >  1:686470/1‑81 (MQ=255)                                       aaGCCAACGGGCGCACAGGCACGCGTAGTGGCGGAGTTCGAGCGCGATATGGCGCTGGATGTGCCGATGATGCGTCTGGTg                                   <  1:686390/81‑1 (MQ=255)                                       aaGCCAACGGGCGCACAGGCACGCGTAGTGGCGGAGTTCGAGCGCGATATGGCGCTGGATGTGCCGATGATGCGTCTGGTg                                   <  2:686388/81‑1 (MQ=255)                                        aGCCAACGGGCGCACAGGCACGCGTAGTGGCGGAGTTCGAGCGCGATATGGCGCTGGATGTGCCGATGATGCGTCTGGTg                                   >  2:686473/1‑80 (MQ=255)                                        aGCCAACGGGCGCACAGGCACGCGTAGTGGCGGAGTTCGAGCGCGATATGGCGCTGGATGTGCCGATGATGCGTCTGGTGc                                  <  2:686391/81‑1 (MQ=255)                                         gCCAACGGGCGCACAGGCACGCGTAGTGGCGAAGTTCGAGCGCGATATGGCGCTGGATGTGCCGATGATGCGTCTGGTGCa                                 <  2:686392/81‑1 (MQ=255)                                          ccAACGGGCGCACAGGCACGCGTAGTGGCGGAGTTCGAGCGCGATATGGCGCTGGATGTGCCGATGATGCGTCTGGTGCAg                                <  2:686393/81‑1 (MQ=255)                                           cAACGGGCGCACAGGCACGCGTAGTGGCGGAGTTCGAGCGCGATATGGCGCTGGATGTGCCGATGATGCGTCTGGTGCAgg                               >  2:686461/1‑81 (MQ=255)                                           cAACGGGCGCACAGGCACGCGTAGTGGCGGAGTTCGAGCGCGATATGGCGCTGGATGTGCCGATGATGCGTCTGGTGCAgg                               >  2:686450/1‑81 (MQ=255)                                            aaCGGGCGCACAGGCACGCGTAGTGGCGGAGTTCGAGCGCGATATGGCGCTGGATGTGCCGATGATGCGTCTGGTGCAggg                              <  2:686394/81‑1 (MQ=255)                                            aaCGGGCGCACAGGCACGCGTAGTGGCGGAGTTCGAGCGCGATATGGCGCTGGATGTGCCGATGATGCGTCTGGTGCAggg                              <  1:686396/81‑1 (MQ=255)                                               gggCGCACAGGCACGCGTAGTGGCGGAGTTCGAGCGCGATCTGGCGCTGGATGTGCCGATGATGCGTCTGGTGCAGGGCGa                           >  2:686460/1‑81 (MQ=255)                                                     acaGGCACGCGTAGTGGCGGAGTTCGAGCGCGATATGGCGCTGGATGTGCCGATGATGCGTCTGGTGCAGGGCGATGTAgg                     >  1:686463/1‑81 (MQ=255)                                                      caGGCACGCGTAGTGGCGGAGTTCGAGCGCGATATGGCGCTGGATGTGCCGATGATGCGTCTGGTGCAGGGCGATGTAGGt                    <  1:686397/81‑1 (MQ=255)                                                       aGGCACGCGTAGTGGCGGAGTTCGAGCGCGATATGGCGCTGGATGTGCCGATGATGCGTCTGGTGCAGGGCGATGTAGGtt                   >  2:686466/1‑81 (MQ=255)                                                        ggCACGCGTAGTGGCGGAGTTCGAGCGCGATATGGCGCTGGATGTGCCGATGATGCGTCTGGTGCAGGGCGATGTAGGTTc                  <  1:686398/81‑1 (MQ=255)                                                             gcgTAGTGGCGGAGTTCGAGCGCGATATGGCGCTGGATGTGCCGATGATGCGTCTGGTGCAGGGCGATGTAGGTTCCGGt              >  1:686459/1‑80 (MQ=255)                                                             gcgTAGTGGCGGAGTTCGAGCGCGATATGGCGCTGGATGTGCCGATGATGCGTCTGGTGCAGGGCGATGTAGGTTCCGGTa             <  1:686399/81‑1 (MQ=255)                                                              cgTAGTGGCGGAGTTCGAGCGCGATATGGCGCTGGATGTGCCGATGATGCGTCTGGTGCAGGGCGATGTAGGTTCCGGTa             >  1:686477/1‑80 (MQ=255)                                                              cgTAGTGGCGGAGTTCGAGCGCGATATGGCGCTGGATGTGCCGATGATGCGTCTGGTGCAGGGCGATGTAGGTTCCGGTa             >  2:686484/1‑80 (MQ=255)                                                               gTAGTGGCGGAGTTCGAGCGCGATATGGCGCTGGATGTGCCGATGATGCGTCTGGTGCAGGGCGATGTAGGTTCCGGTaaa           <  1:686400/81‑1 (MQ=255)                                                                tAGTGGCGGAGTTCGAGCGCGATATGGCGCTGGATGTGCCGATGATGCGTCTGGTGCAGGGCGATGTAGGTTCCGGTaaaa          >  2:686485/1‑81 (MQ=255)                                                                 aGTGGCGGAGTTCGAGCGCGATATGGCGCTGGATGTGCCGATGATGCGTCTGGTGCAGGGCGATGTAGGTTCCGGTAAAAc         >  1:686475/1‑81 (MQ=255)                                                                  gTGGCGGAGTTCGAGCGCGATATGGCGCTGGATGTGCCGATGATGCGTCTGGTGCAGGGCGATGTAGGTTCCGGTAAAAc         <  2:686400/80‑1 (MQ=255)                                                                  gTGGCGGAGTTCGAGCGCGATATGGCGCTGGATGTGCCGATGATGCGTCTGGTGCAGGGCGATGTAGGTTCCGGTAAAACg        <  2:686401/81‑1 (MQ=255)                                                                  gTGGCGGAGTTCGAGCGCGATATGGCGCTGGATGTGCCGATGATGCGTCTGGTGCAGGGCGATGTAGGTTCCGGTAAAACg        >  2:686482/1‑81 (MQ=255)                                                                       ggAGTTCGAGCGCGATATGGCGCTGGATGTGCCGATGATGCGTCTGGTGCAGGGCGATGTAGGTTCCGGTAAAACGCTGGt   >  2:686481/1‑81 (MQ=255)                                                                        gagtTCGAGCGCGATATGGCGCTGGATGTGCCGATGATGCGTCTGGTGCAGGGCGATGTAGGTTCCGGTAAAACGCTGGTc  <  1:686403/81‑1 (MQ=255)                                                                           |                                                                              ACACGCTGAAAAATAAACTCCTCGCCGCCTTACCGTTCAAGCCAACGGGCGCACAGGCACGCGTAGTGGCGGAGATCGAGCGCGATATGGCGCTGGATGTGCCGATGATGCGTCTGGTGCAGGGCGATGTAGGTTCCGGTAAAACGCTGGTC  >  NC_000913/3825973‑3826124

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑