Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,833,590	G→A	C316C (TGC→TGT)	yicI ←	alpha‑D‑xyloside xylohydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,833,590	0	G	A	100.0%	133.7 / NA	38	C316C (TGC→TGT)	yicI	alpha‑D‑xyloside xylohydrolase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (22/16);  total (22/16)

GATTTTCAGTCCTTTCGCTTTCAGGCGGCGGATCATCCCTTCCGGGTCAGGGAAAGTCAGCGGGTCCCACTCAAAATCGCACCACTGGAAGGCTTTCATCCAGAAACAGTCAAAGTGGAAAACATGCAGCGGCAGATTGCGTTCCGCCATACCATC  >  NC_000913/3833512‑3833667                                                                               |                                                                              gATTTTCAGTCCTTTCGCTTTCAGGCGGCGGATCATCCCTTCCGGGTCAGGGAAAGTCAGCGGGTCCCACTCAAAATcaca                                                                             >  1:688743/1‑81 (MQ=255)  aTTTTCAGTCCTTTCGCTTTCAGGCGGCGGATCATCCCTTCCGGGTCAGGGAAAGTCAGCGGGTCCCACTCAAAATCacac                                                                            >  2:688729/1‑81 (MQ=255)  aTTTTCAGTCCTTTCGCTTTCAGGCGGCGGATCATCCCTTCCGGGTCAGGGAAAGTCAGCGGGTCCCACTCAAAATCacac                                                                            <  1:688673/81‑1 (MQ=255)     ttCAGTCCTTTCGCTTTCAGGCGGCGGATCATCCCTTCCGGGTCAGGGAAAGTCAGCGGGTCCCACTCAAAATCAcaccac                                                                         >  2:688732/1‑81 (MQ=255)      tCAGTCCTTTCGCTTTCAGGCGGCGGATCATCCCTTCCGGGTCAGGGAAAGTCAGCGGGTCCCACTCAAAATCAcaccac                                                                         <  2:688673/80‑1 (MQ=255)       cAGTCCTTTCGCTTTCAGGCGGCGGATCATCCCTTCCGGGTCAGGGAAAGTCAGCGGGTCCCACTCAAAATCACACCACTg                                                                       >  2:688726/1‑81 (MQ=255)         gTCCTTTCGCTTTCAGGCGGCGGATCATCCCTTCCGGGTCAGGGAAAGTCAGCGGGTCCCACTCAAAATCACACCACTgg                                                                      >  1:688717/1‑80 (MQ=255)         gTCCTTTCGCTTTCAGGCGGCGGATCATCCCTTCCGGGTCAGGGAAAGTCAGCGGGTCCCACTCAAAATCACACCACTGGa                                                                     <  2:688674/81‑1 (MQ=255)            cTTTCGCTTTCAGGCGGCGGATCATCCCTTCCGGGTCAGGGAAAGTCAGCGGGTCCCACTCAAAATCACACCACTGGAAgg                                                                  >  2:688745/1‑81 (MQ=255)               tCGCTTTCAGGCGGCGGATCATCCCTTCCGGGTCAGGGAAAGTCAGCGGGTCCCACTCAAAATCACACCACTGGAAGGc                                                                 <  1:688676/79‑1 (MQ=255)                cGCTTTCAGGCGGCGGATCATCCCTTCCGGGTCAGGGAAAGTCAGCGGGTCCCACTCAAAATCACACCACTGGAAGGCtt                                                               <  1:688677/80‑1 (MQ=255)                 gCTTTCAGGCGGCGGATCATCCCTTCCGGGTCAGGGAAAGTCAGCGGGTCCCACTCAAAATCACACCACTGGAAGGCttt                                                              >  2:688723/1‑80 (MQ=255)                 gCTTTCAGGCGGCGGATCATCCCTTCCGGGTCAGGGAAAGTCAGCGGGTCCCACTCAAAATCACACCACTGGAAGGCttt                                                              >  2:688714/1‑80 (MQ=255)                     tCAGGCGGCGGATCATCCCTTCCGGGTCAGGGAAAGTCAGCGGGTCCCACTCAAAATCACACCACTGGAAGGCTTTCATc                                                          >  1:688728/1‑80 (MQ=255)                       aGGCGGCGGATCATCCCTTCCGGGTCAGGGAAAGTCAGCGGGTCCCACTCAAAATCACACCACTGGAAGGCTTTCATc                                                          >  1:688725/1‑78 (MQ=255)                       aGGCGGCGGATCATCCCTTCCGGGTCAGGGAAAGTCAGCGGGTCCCACTCAAAATCACACCACTGGAAGGCTTTCATCCAg                                                       <  2:688677/81‑1 (MQ=255)                             cggATCATCCCTTCCGGGTCAGGGAAAGTCAGCGGGTCCCACTCAAAATCACACCACTGGAAGGCTTTCATCCAGAAACAg                                                 <  2:688678/81‑1 (MQ=255)                              ggATCATCCCTTCCGGGTCAGGGAAAGTCAGCGGGTCCCACTCAAAATCACACCACTGGAAGGCTTTCATCCAGAAACAGt                                                <  1:688680/81‑1 (MQ=255)                                atcatcCCTTCCGGGTCAGGGAAAGTCAGCGGGTCCCACTCAAAATCACACCACTGGAAGGCTTTCATCCAGAAACAGTc                                               >  1:688722/1‑80 (MQ=255)                                  catcCCTTCCGGGTCAGGGAAAGTCAGCGGGTCCCACTCAAAATCACACCACTGGAAGGCTTTCATCCAGAAACAGTCa                                              >  2:688728/1‑79 (MQ=255)                                     cccTTCCGGGTCAGGGAAAGTCAGCGGGTCCCACTCAAAATCACACCACTGGAAGGCTTTCATCCAGAAACAGTCAAAGTg                                         >  1:688740/1‑81 (MQ=255)                                     cccTTCCGGGTCAGGGAAAGTCAGCGGGTCCCACTCAAAATCACACCACTGGAAGGCTTTCATCCAGAAACAGTCAAAGTg                                         >  1:688731/1‑81 (MQ=255)                                     cccTTCCGGGTCAGGGAAAGTCAGCGGGTCCCACTCAAAATCACACCACTGGAAGGCTTTCATCCAGAAACAGTCAAAGTg                                         >  1:688737/1‑81 (MQ=255)                                          ccGGGTCAGGGAAAGTCAGCGGGTCCCACTCAAAATCACACCACTGGAAGGCTTTCATCCAGAAACAGTCAAAGTGGaaa                                     <  1:688681/80‑1 (MQ=255)                                           cGGGTCAGGGAAAGTCAGCGGGTCCCACTCAAAATCACACCACTGGAAGGCTTTCATCCAGAAACAGTCAAAGTGGaaaa                                    >  1:688758/1‑80 (MQ=255)                                                cAGGGAAAGTCAGCGGGTCCCACTCAAAATCACACCACTGGAAGGCTTTCATCCAGAAACAGTCAAAGTGGAAAACATg                                >  2:688734/1‑79 (MQ=255)                                                  gggAAAGTCAGCGGGTCCCACTCAAAATCACACCACTGGAAGGCTTTCATCCAGAAACAGTCAAAGTGGAAAACATGCAGc                            >  2:688741/1‑81 (MQ=255)                                                   ggAAAGTCAGCGGGTCCCACTCAAAATCACACCACTGGAAGGCTTTCATCCAGAAACAGTCAAAGTGGAAAACATGCAGCg                           <  2:688681/81‑1 (MQ=255)                                                       aGTCAGCGGGTCCCACTCAAAATCACACCACTGGAAGGCTTTCATCCAGAAACAGTCAAAGTTGAAAACATGCAGCGGCAg                       <  2:688682/81‑1 (MQ=255)                                                          cAGCGGGTCCCACTCAAAATCACACCACTGGAAGGCTTTCATCCAGAAACAGTCAAAGTGGAAAACATGCAGCGGCAGAtt                    >  2:688733/1‑81 (MQ=255)                                                            gCGGGTCCCACTCAAAATCACACCACTGGAAGGCTTTCATCCAGAAACAGTCAAAGTGGAAAACATGCAGCGGCAGATTGc                  <  2:688683/81‑1 (MQ=255)                                                            gCGGGTCCCACTCAAAATCACACCACTGGAAGGCTTTCATCCAGAAACAGTCAAAGTGGAAAACATGCAGCGGCAGATTGc                  <  1:688685/81‑1 (MQ=255)                                                             cGGGTCCCACTCAAAATCACACCACTGGAAGGCTTTCATCCAGAAACAGTCAAAGTGGAAAACATGCAGCGGCAGATTGCg                 >  2:688747/1‑81 (MQ=255)                                                              gggTCCCACTCAAAATCACACCACTGGAAGGCTTTCATCCAGAAACAGTCAAAGTGGAAAACATGCAGCGGCAGATTGCGt                <  2:688685/81‑1 (MQ=255)                                                               ggTCCCACTCAAAATCACACCACTGGAAGGCTTTCATCCAGAAACAGTCAAAGTGGAAAACATGCAGCGGCAGATTGCGtt               <  2:688686/81‑1 (MQ=255)                                                                gTCCCACTCAAAATCACACCACTGGAAGGCTTTCATCCAGAAACAGTCAAAGTGGAAAACATGCAGCGGCAGATTGCGTTc              >  1:688738/1‑81 (MQ=255)                                                                 tCCCACTCAAAATCACACCACTGGAAGGCTTTCATCCAGAAACAGTCAAAGTGGAAAACATGCAGCGGCAGATTGCGTTcc             <  2:688688/81‑1 (MQ=255)                                                                 tCCCACTCAAAATCACACCACTGGAAGGCTTTCATCCAGAAACAGTCAAAGTGGAAAACATGCAGCGGCAGATTGCGTTcc             <  2:688687/81‑1 (MQ=255)                                                                         aaaaTCACACCACTGGAAGGCTTTCATCCAGAAACAGTCAAAGTGGAAATCATGCAGCGGCAGATTGCGTTCCGCCATAc      >  2:688753/1‑80 (MQ=255)                                                                         aaaaTCACACCACTGGAAGGCTTTCATCCAGAAACAGTCAAAGTGGAAAACATGCAGCGGCAGATTGCGTTCCGCCATAc      >  2:688744/1‑80 (MQ=255)                                                                            aTCACACCACTGGAAGGCTTTCATCCAGAAACAGTCAAAGTGGAAAACATGCAGCGGCAGATTGCGTTCCGCCATACCATc  >  1:688739/1‑81 (MQ=255)                                                                               |                                                                              GATTTTCAGTCCTTTCGCTTTCAGGCGGCGGATCATCCCTTCCGGGTCAGGGAAAGTCAGCGGGTCCCACTCAAAATCGCACCACTGGAAGGCTTTCATCCAGAAACAGTCAAAGTGGAAAACATGCAGCGGCAGATTGCGTTCCGCCATACCATC  >  NC_000913/3833512‑3833667

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑