Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	387,413	T→A	L148Q (CTG→CAG)	tauC →	taurine ABC transporter membrane subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	387,413	0	T	A	100.0%	74.9 / NA	22	L148Q (CTG→CAG)	tauC	taurine ABC transporter membrane subunit
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (14/8);  total (14/8)

TCGTCCGGTGCCGCCGCTGGCTTATTTGCCGCTGATGGTGATCTGGTTTGGTATTGGTGAAACCTCGAAGATCTTACTGATCTATTTAGCGATTTTTGCACCGGTGGCGATGTCGGCGCTGGCGGGGGTGAAAAGCGTGCAGCAGGT  >  NC_000913/387336‑387482                                                                              |                                                                      tCGTCCGGTGCCGCCGCTGGCTTATTTGCCGCTGATGGTGATCTGGTTTGGTATTGGTGAAACCTCGAAGATCTTACAGAt                                                                    <  1:79448/81‑1 (MQ=255)   ttCCGGTGCCGCCGCTGTCTTATTTGCCGCTGATGGTGATCTGGTTTGGTATTGGTGAAACCTCGACGATCTTACAGATc                                                                   >  2:79485/2‑80 (MQ=255)     ccGGTGCCGCCGCTGGCTTATTTGCCGCTGATGGTGATCTGGTTTGGTATTGGTGAAACCTCGAAGATCTTACAGATCTAt                                                                >  1:79478/1‑81 (MQ=255)       ggTGCCGCCGCTGGCTTATTTGCCGCTGATGGTGATCTGGTTTGGTATTGGTGAAACCTCGAAGATCTTACAGATCTAtt                                                               >  2:79481/1‑80 (MQ=255)        gTGCCGCCGCTGGCTTATTTGCCGCTGATGGTGATCTGGTTTGGTATTGGTGAAACCTCGAAGATCTTACAGATCTAtt                                                               >  2:79465/1‑79 (MQ=255)             gccgcTGGCTTATTTGCCGCTGATGGTGATCTGGTTTGGTATTGGTGAAACCTCGAAGATCTTACAGATCTATTTAGCGAt                                                        >  2:79480/1‑81 (MQ=255)             gccgcTGGCTTATTTGCCGCTGATGGTGATCTGGTTTGGTATTGGTGAAACCTCGAAGATCTTACAGATCTATTTAGCGAt                                                        <  2:79449/81‑1 (MQ=255)                 cTGGCTTATTTGCCGCTGATGGTGATCTGGTTTGGTATTGGTGAAACCTCGAAGATCTTACAGATCTATTTAGCGAttttt                                                    <  1:79450/81‑1 (MQ=255)                   ggCTTATTTGCCGCTGATGGTGATCTGGTTTGGTATTGGTGAAACCTCGAAGATCTTACAGATCTATTTAGCGATTTTTg                                                   >  2:79486/1‑80 (MQ=255)                            gCCGCTGATGGTGATCTGGTTTGGTATTGGTGAAACCTCGAAGATCTTACAGATCTATTTAGCGATTTTTGCACCGGTgg                                          <  1:79451/80‑1 (MQ=255)                                cTGATGGTGATCTGGTTTGGTATTGGTGAAACCTCGAAGATCTTACAGATCTATTTAGCGATTTTTGCACCGGTGGCGATg                                     >  1:79496/1‑81 (MQ=255)                                cTGATGGTGATCTGGTTTGGTATTGGTGAAACCTCGAAGATCTTACAGATCTATTTAGCGATTTTTGCACCGGTGGCGATg                                     <  2:79452/81‑1 (MQ=255)                                   aTGGTGATCTGGTTTGGTATTGGTGAAACCTCGAAGATCTTACAGATCTATTTAGCGATTTTTGCACCGGTGGCGATGTCg                                  >  2:79494/1‑81 (MQ=255)                                   aTGGTGATCTGGTTTGGTATTGGTGAAACCTCGAAGATCTTACAGATCTATTTAGCGATTTTTGCACCGGTGGCGATGTCg                                  >  1:79484/1‑81 (MQ=255)                                         aTCTGGTTTGGTATTGGTGAAACCTCGAAGATCTTACAGATCTATTTAGCGATTTTTGCACCGGTGGCGATGTCGGCGCTg                            >  1:79530/1‑81 (MQ=255)                                            tGGTTTGGTATTGGTGAAACCTCGAAGATCTTACAGATCTATTTAGCGATTTTTGCACCGGTGGCGATGTCGGCGCTGGCg                         >  1:79487/1‑81 (MQ=255)                                              gTTTGGTATTGGTGAAACCTCGAAGATCTTACAGATCTATTTAGCGATTTTTGCACCGGTGGCGATGTCGGCGCTg                            <  1:79453/76‑1 (MQ=255)                                                       tGGTGAAACCTCGAAGATCTTACAGATCTATTTAGCGATTTTTGCACCGGTGGCGATGTCGGCGCTGGCGGGGGTGAAAAg              >  2:79489/1‑81 (MQ=255)                                                               ccTCGAAGATCTTACAGATCTATTTAGCGATTTTTGCACCGGTGGCGATGTCGGCGCTGGCGGGGGTGAAAAGCGTgcagc      <  2:79454/81‑1 (MQ=255)                                                               ccTCGAAGATCTTACAGATCTATTTAGCGATTTTTGCACCGGTGGCGATGTCGGCGCTGGCGGGGGTGAAAAGCGTgcagc      <  2:79455/81‑1 (MQ=255)                                                                cTCGAAGATCTTACAGATCTATTTAGCGATTTTTGCACCGGTGGCGATGTCGGCGCTGGCGGGGGTGAAAAGCGTgcag       >  2:79491/1‑79 (MQ=255)                                                                   gAAGATCTTACAGATCTATTTAGCGATTTTTGCACCGGTGGCGATGTCGGCGCTGGCGGGGGTGAAAAGCGTGCAGCAGGt  >  2:79495/1‑81 (MQ=255)                                                                              |                                                                      TCGTCCGGTGCCGCCGCTGGCTTATTTGCCGCTGATGGTGATCTGGTTTGGTATTGGTGAAACCTCGAAGATCTTACTGATCTATTTAGCGATTTTTGCACCGGTGGCGATGTCGGCGCTGGCGGGGGTGAAAAGCGTGCAGCAGGT  >  NC_000913/387336‑387482

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑