Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,863,817	A→T	intergenic (‑214/‑82)	glvC ← / → yidP	putative PTS enzyme II component GlvC/putative DNA‑binding transcriptional regulator YidP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,863,817	0	A	T	100.0%	96.5 / NA	27	intergenic (‑214/‑82)	glvC/yidP	putative PTS enzyme II component GlvC/putative DNA‑binding transcriptional regulator YidP
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (14/13);  total (14/13)

TGGGCGGTAGAGATCTACGTTTTCGCGAAAAAGTAGACCTCTGTGAGCACAGGTACACAGCATCGAACACATCTTTAAAAAAAAGATGTTTTTTCAATCGATTAAGCAGAACTTGTGTGCGCATTACCCGGGCTTGCAGGCAAAAAAGAGATC  >  NC_000913/3863741‑3863893                                                                             |                                                                             tGGGCGGTAGAGATCTACGTTTTCGCGAAAAAGTAGACCTCTGTGAGCACAGGTACACAGCATCGAACACATCTTTtaaa                                                                           >  2:696949/1‑80 (MQ=255)          gagaTCTACGTTTTCGCGAAAAAGTAGACCTCTGTGAGCACAGGTACACAGCATCGAACACATCTTTTAAAAAAAGATGt                                                                  <  2:696892/80‑1 (MQ=255)           agaTCTACATTTTCGCGAAAAAGTAGACCTCTGTGAGCACAGGTACACAGCATCGAACACATCTTTTAAAAAAAGATGttt                                                                <  1:696894/81‑1 (MQ=255)            gaTCTACGTTTTCGCGAAAAAGTAGACCTCTATGAGCACAGGTACACAGCATCGAACACATCTTTTAAAAAAAGATGtttt                                                               <  2:696894/81‑1 (MQ=255)                  cGTTTTCGCGAAAAAGTAGACCTCTGTGAGCACAGGTACACAGCATCGAACACATCTTTTAAAAAAAGATGttttt                                                              <  1:696896/76‑1 (MQ=255)                  cGTTTTCGCGAAAAAGTAGACCTCTGTGAGCACAGGTACACAGCATCGAACACATCTTTTAAAAAAAGATGTTTTTTCaa                                                          <  2:696897/80‑1 (MQ=255)                  cGTTTTCGCGAAAAAGTAGACCTCTGTGAGCACAGGTACACAGCATCGAACACATCTTTTAAAAAAAGATGTTTTTTCa                                                           <  2:696896/79‑1 (MQ=255)                    ttttCGCGAAAAAGTAGACCTCTGTGAGCACAGGTACACAGCATCGAACACATCTTTTAAAAAAAGATGTTTTTTCAATc                                                        >  1:696943/1‑80 (MQ=255)                     tttCGCGAAAAAGTAGACCTCTGTGAGCACAGGTACACAGCATCGAACACATCTTTTAAAAAAAGATGTTTTTTCAATCGa                                                      >  1:696944/1‑81 (MQ=255)                     tttCGCGAAAAAGTAGACCTCTGTGAGCACAGGTACACAGCATCGAACACATCTTTTAAAAAAAGATGTTTTTTCAATCGa                                                      >  2:696948/1‑81 (MQ=255)                      ttCGCGAAAAAGTAGACCTCTGTGAGCACAGGTACACAGCATCGAACACATCTTTTAAAAAAAGATGTTTTTTCAATCGa                                                      <  1:696899/80‑1 (MQ=255)                         gcgAAAAAGTAGACCTCTGTGAGCACAGGTACACAGCATCGAACACATCTTTTAAAAAAAGATGTTTTTTCAATCGATTa                                                   <  1:696900/80‑1 (MQ=255)                           gAAAAAGTAGACCTCTGTGAGCACAGGTACACAGCATCGAACACATCTTTTAAAAAAAGATGTTTTTTCAATCGATTAAg                                                 >  2:696946/1‑80 (MQ=255)                            aaaaaGTAGACCTCTGTGAGCACAGGTACACAGCATCGAACACATCTTTTAAAAAAAGATGTTTTTTCAATCGATTAAg                                                 >  1:696951/1‑79 (MQ=255)                                aGTAGACCTCTGTGAGCACAGGTACACAGCATCGAACACATCTTTTAAAAAAAGATGTTTTTTCAATCGATTAAGCAGAAc                                           >  1:696945/1‑81 (MQ=255)                                aGTAGACCTCTGTGAGCACAGGTACACAGCATCGAACACATCTTTTAAAAAAAGATGTTTTTTCAATCGATTAAGCAGAAc                                           >  2:696941/1‑81 (MQ=255)                                 gTAGACCTCTGTGAGCACAGGTACACAGCATCGAACACATCTTTTAAAAAAAGATGTTTTTTCAATCGATTAAGCAGAACt                                          <  2:696900/81‑1 (MQ=255)                                  tAGACCTCTGTGAGCACAGGTACACAGCATCGAACACATCTTTTAAAAAAAGATGTTTTTTCAATCGATTAAGCAGAACtt                                         <  2:696901/81‑1 (MQ=255)                                   aGACCTCTGTGAGCACAGGTACACAGCATCGAACACATCTTTTAAAAAAAGATGTTTTTTCAATCGATTAAGCAGAACTtg                                        >  1:696948/1‑81 (MQ=255)                                       ctctGTGAGCACAGGTACACAGCATCGAACACATCTTTTAAAAAAAGATGTTTTTTCAATCGATTAAGCAGAACTtgtgtg                                    >  1:696952/1‑81 (MQ=255)                                       ctctGTGAGCACAGGTACACAGCATCGAACACATCTTTTAAAAAAAGATGTTTTTTCAATCGATTAAGCAGAACTtgtgtg                                    >  2:696945/1‑81 (MQ=255)                                            tgAGCACAGGTACACAGCATCGAACACATCTTTTAAAAAAAGATGTTTTTTCAATCGATTAAGCAGAACTTGTGTGCGCAt                               <  2:696902/81‑1 (MQ=255)                                                 acaGGTACACAGCATCGAACACATCTTTTAAAAAAAGATGTTTTTTCAATCGATTAAGCAGAACTTGTGTGCGCATTAccc                          >  1:696970/1‑81 (MQ=255)                                                 acaGGTACACAGCATCGAACACATCTTTTAAAAAAAGATGTTTTTTCAATCGATTAAGCAGAACTTGTGTGCGCATTAccc                          <  1:696904/81‑1 (MQ=255)                                                         acaGCATCGAACACATCTTTTAAAAAAAGATGTTTTTTCAATCGATTAAGCAGAACTTGTGTGCGCATTACCCGGGCTTg                   >  1:696962/1‑80 (MQ=255)                                                               tCGAACACATCTTTTAAAAAAAGATGTTTTTTCAATCGATTAAGCAGAACTTGTGTGCGCATTACCCGGGCTTGCAGGCaa            <  2:696904/81‑1 (MQ=255)                                                                       aTCTTTTAAAAAAAGATGTTTTTTCAATCGATTAAGCAGAACTTGTGTGCGCATTACCCGGGCTTGCAGGCaaaaaa        >  1:696971/1‑77 (MQ=255)                                                                         cTTTTAAAAAAAGATGTTTTTTCAATCGATTAAGCAGAACTTGTGTGCGCATTACCCGGGCTTGCAGGCAAAAAAGAGATc  >  2:696952/1‑81 (MQ=255)                                                                             |                                                                             TGGGCGGTAGAGATCTACGTTTTCGCGAAAAAGTAGACCTCTGTGAGCACAGGTACACAGCATCGAACACATCTTTAAAAAAAAGATGTTTTTTCAATCGATTAAGCAGAACTTGTGTGCGCATTACCCGGGCTTGCAGGCAAAAAAGAGATC  >  NC_000913/3863741‑3863893

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑