Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,866,522	G→C	P126A (CCC→GCC)	ibpB ←	small heat shock protein IbpB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,866,522	0	G	C	100.0%	126.5 / NA	36	P126A (CCC→GCC)	ibpB	small heat shock protein IbpB
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (20/16);  total (20/16)

GCGGGGCAAAGAGAATAGCTAGTTAGCTATTTAACGCGGGACGTTCGCTGATAGCGATACGCTGCGCTGCGATGGGTTCAGGCTCATTACGAATTAAATCAATATGCAGTAAACCGTTTACGAAGGTTGCGCCAGAGACTTCCATATTTT  >  NC_000913/3866447‑3866596                                                                            |                                                                           gCGGGGCAAAGAGAATAGCTAGTTAGCTATTTAACGCGGGACGTTCGCTGATAGCGATACGCTGCGCTGCGATGGCTTCa                                                                        <  2:697621/80‑1 (MQ=255) gCGGGGCAAAGAGAATAGCTAGTTAGCTATTTAACGCGGGACGTTCGCTGATAGCGATACGCTGCGCTGCGATGGCTTCAg                                                                       <  1:697621/81‑1 (MQ=255)   ggggCAAAGAGAATAGCTAGTTAGCTATTTAACGCGGGACGTTCGCTGATAGCGATACGCTGCGCTGCGATGGCTTCAGGc                                                                     >  2:697671/1‑81 (MQ=255)    gggCAAAGAGAATAGCTAGTTAGCTATTTAACGCGGGACGTTCGCTGATAGCGATACGCTGCGCTGCGATGGCTTCAGGCt                                                                    <  2:697622/81‑1 (MQ=255)    gggCAAAGAGAATAGCTAGTTAGCTATTTAACGCGGGACGTTCGCTGATAGCGATACGCTGCGCTGCGATGGCTTCAGGCt                                                                    >  1:697688/1‑81 (MQ=255)       cAAAGAGAATAGCTAGTTAGCTATTTAACGCGGGACGTTCGCTGATAGCGATACGCTGCGCTGCGATGGCTTCAGGCTCAt                                                                 >  1:697678/1‑81 (MQ=255)        aaaGAGAATAGCTAGTTAGCTATTTAACGCGGGACGTTCGCTGATAGCGATACGCTGCGCTGCGATGGCTTCAGGCTCAtt                                                                <  1:697624/81‑1 (MQ=255)         aaGAGAATAGCTAGTTAGCTATTTAACGCGGGACGTTCGCTGATAGCGATACGCTGCGCTGCGATGGCTTCAGGCTCATTa                                                               >  1:697685/1‑81 (MQ=255)          agagAATAGCTAGTTAGCTATTTAACGCGGGACGTTCGCTGATAGCGATACGCTGCGCTGCGATGGCTTCAGGCTCATTAc                                                              >  1:697666/1‑81 (MQ=255)            agaCTAGCTAGTTAGCTATTTAACGCGAGACGTTCGCTGATAGCGATACGCTGCGCTGCGATGGCTTCAGGCTCATTACGa                                                            <  2:697624/81‑1 (MQ=255)             gaATAGCTAGTTAGCTATTTAACGCGGGACGTTCGCTGATAGCGATACGCTGCGCTGCGATGGCTTCAGGCTCATTACGaa                                                           >  1:697668/1‑81 (MQ=255)              aaTAGCTAGTTAGCTATTTAACGCGGGACGTTCGCTGATAGCGATACGCTGCGCTGCGATGGCTTCAGGCTCATTACGAAt                                                          <  2:697625/81‑1 (MQ=255)               aTAGCTAGTTAGCTATTTAACGCGGGACGTTCGCTGATAGCGATACGCTGCGCTGCGATGGCTTCAGGCTCATTACGAAtt                                                         <  2:697626/81‑1 (MQ=255)                  gCTAGTTAGCTATTTAACGCGGGACGTTCGCTGATAGCGATACGCTGCGCTGCGATGGCTTCAGGCTCATTACGAATTaaa                                                      >  1:697697/1‑81 (MQ=255)                     aGTTAGCTATTTAACGCGGGACGTTCGCTGATAGCGATACGCTGCGCTGCGATGGCTTCAGGCTCATTACGAATTAAATc                                                    >  2:697682/1‑80 (MQ=255)                     aGTTAGCTATTTAACGCGGGACGTTCGCTGATAGCGATACGCTGCGCTGCGATGGCTTCAGGCTCATTACGAATTAAATc                                                    >  2:697686/1‑80 (MQ=255)                           cTATTTAACGCGGGACGTTCGCTGATAGCGATACGCTGCGCTGCGATGGCTTCAGGCTCATTACGAATTAAATCAATATGc                                             <  1:697628/81‑1 (MQ=255)                           cTATTTAACGCGGGACGTTCGCTGATAGCGATACGCTGCGCTGCGATGGCTTCAGGCTCATTACGAATTAAATCAATATGc                                             <  2:697628/81‑1 (MQ=255)                              tttAACGCGGGACGTTCGCTGATAGCGATACGCTGCGCTGCGATGGCTTCAGGCTCATTACGAATTAAATCAATATGCAGt                                          <  2:697629/81‑1 (MQ=255)                                tAACGCGGGACGTTCGCTGATAGCGATACGCTGCGCTGCGATGGCTTCAGGCTCATTACGAATTAAATCAATATGCAGTa                                         >  1:697684/1‑80 (MQ=255)                                  aCGCGGGACGTTCGCTGATAGCGATACGCTGCGCTGCGATGGCTTCAGGCTCATTACGAATTAAATCAATATGCAGTAAAc                                      <  1:697631/81‑1 (MQ=255)                                   cgcgGGACGTTCGCTGATAGCGATACGCTGCGCTGCGATGGCTTCAGGCTCATTACGAATTAAATCAATATGCAGTAAAcc                                     >  1:697677/1‑81 (MQ=255)                                   cgcgGGACGTTCGCTGATAGCGATACGCTGCGCTGCGATGGCTTCAGGCTCATTACGAATTAAATCAATATGCAGTAAAcc                                     >  2:697680/1‑81 (MQ=255)                                    gcgGGACGTTCGCTGATAGCGATACGCTGCGCTGCGATGGCTTCAGGCTCATTACGAATTAAATCAATATGCAGTAAACCg                                    >  2:697672/1‑81 (MQ=255)                                      gggACGTTCGCTGATAGCGATACGCTGCGCTGCGATGGCTTCAGGCTCATTACGAATTAAATCAATATGCAGTAAACCGtt                                  <  2:697631/81‑1 (MQ=255)                                       ggACGTTCGCTGATAGCGATACGCTGCGCTGCGATGGCTTCAGGCTCATTACGAATTAAATCAATATGCAGTAAACCGttt                                 <  1:697633/81‑1 (MQ=255)                                       ggACGTTCGCTGATAGCGATACGCTGCGCTGCGATGGCTTCAGGCTCATTACGAATTAAATCAATATGCAGTAAACCGttt                                 <  1:697634/81‑1 (MQ=255)                                         aCGTTCGCTGATAGCGATACGCTGCGCTGCGATGGCTTCAGGCTCATTACGAATTAAATCAATATGCAGTAAACCGTTTAc                               >  2:697678/1‑81 (MQ=255)                                          cGTTCGCTGATAGCGATACGCTGCGCTGCGATGGCTTCAGGCTCATTACGAATTAAATCAATATGCAGTAAACCGTTTACg                              <  2:697634/81‑1 (MQ=255)                                            ttCGCTGATAGCGATACGCTGCGCTGCGATGGCTTCAGGCTCATTACGAATTAAATCAATATGCAGTAAACCGTTTACg                              >  1:697669/1‑79 (MQ=255)                                              cGCTGATAGCGATACGCTGCGCTGCGATGGCTTCAGGCTCATTACGAATTAAATCAATATGCAGTAAACCGTTTACGAAgg                          >  1:697693/1‑81 (MQ=255)                                                cTGATAGCGATACGCTGCGCTGCGATGGCTTCAGGCTCATTACGAATTAAATCAATATGCAGTAAACCGTTTACGAAGGtt                        <  1:697636/81‑1 (MQ=255)                                                  gATAGCGATACGCTGCGCTGCGATGGCTTCAGGCTCATTACGAATTAAATCAATATGCAGTAAACCGTTTACGAAGGTTgc                      >  2:697679/1‑81 (MQ=255)                                                             gctgcgctgcGATGGCTTCAGGCTCATTACGAATTAAATCAATATGCAGTAAACCGTTTACGAAGGTTGCGCCAGAGACtt           >  2:697700/1‑81 (MQ=255)                                                                  gctgcgATGGCTTCAGGCTCATTACGAATTAAATCAATATGCAGTAAACCGTTTACGAAGGTTGCGCCAGAGACTTCCata      >  1:697686/1‑81 (MQ=255)                                                                       gATGGCTTCAGGCTCATTACGAATTAAATCAATATGCAGTAAACCGTTTACGAAGGTTGCGCCAGAGACTTCCATAtttt  >  2:697689/1‑80 (MQ=255)                                                                            |                                                                           GCGGGGCAAAGAGAATAGCTAGTTAGCTATTTAACGCGGGACGTTCGCTGATAGCGATACGCTGCGCTGCGATGGGTTCAGGCTCATTACGAATTAAATCAATATGCAGTAAACCGTTTACGAAGGTTGCGCCAGAGACTTCCATATTTT  >  NC_000913/3866447‑3866596

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑