Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,869,009	G→A	H101Y (CAT→TAT)	yidR ←	DUF3748 domain‑containing galacturonate catabolism protein YidR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,869,009	0	G	A	100.0%	177.4 / NA	49	H101Y (CAT→TAT)	yidR	DUF3748 domain‑containing galacturonate catabolism protein YidR
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (26/23);  total (26/23)

TAATATCCATCGCATCAAGGTTACTCATCTTGCCGCCTTCAACAATCACTCCGCGCCGATGATGGAAGTCGTAATGCCATGTTTCATCAGGATTTTCCGGGCCGTGGATGAAAACATATTTCTCTGACTTCGGATGAACGGTCACCACGCCGA  >  NC_000913/3868934‑3869086                                                                            |                                                                              tAATATCCATCGCATCAAGGTTACTCATCTTGCCGCCTTCAACAATCACTCCGCGCCGATGATGGAAGTCGTAATACCATg                                                                          >  1:698353/1‑81 (MQ=255)      tCCATCGCATCAAGGTTACTCATCTTGCCGCCTTCAACAATCACTCCGCGCCGATGATGGAAGTCGTAATACCATGTTtc                                                                      <  2:698290/80‑1 (MQ=255)       ccATCGCATCAAGGTTACTCATCTTGCCGCCTTCAACAATCACTCCGCGCCGATGATGGAAGTCGTAATACCATGTTtcat                                                                    >  1:698349/1‑81 (MQ=255)        cATCGCATCAAGGTTACTCATCTTGCCGCCTTCAACAATCACTCCGCGCCGATGATGGAAGTCGTAATACCATGTTtcat                                                                    <  2:698291/80‑1 (MQ=255)         aTCGCATCAAGGTTACTCATCTTGCCGCCTTCAACAATCACTCCGCGCCGATGATGGAAGTCGTAATACCATGTTtcat                                                                    >  1:698344/1‑79 (MQ=255)          tCGCATCAAGGTTACTCATCTTGCCGCCTTCAACAATCACTCCGCGCCGATGATGGAAGTCGTAATACCATGTTTCATCAg                                                                 >  2:698339/1‑81 (MQ=255)          tCGCATCAAGGTTACTCATCTTGCCGCCTTCAACAATCACTCCGCGCCGATGATGGAAGTCGTAATACCATGTTTCATCAg                                                                 <  2:698292/81‑1 (MQ=255)             cATCAAGGTTACTCATCTTGCCGCCTTCAACAATCGCTCCGCGCCGATGATGGAAGTCGTAATACCATGTTTCATCAGGAt                                                              <  2:698293/81‑1 (MQ=255)                cAAGGTTACTCATCTTGCCGCCTTCAACAATCACTCCGCGCCGATGATGGAAGTCGTAATACCATGTTTCATCAGGAtttt                                                           >  1:698376/1‑81 (MQ=255)                 aaGGTTACTCATCTTGCCGCCTTCAACAATCACTCCGCGCCGATGATGGAAGTCGTAATACCATGTTTCATCAGGAtttt                                                           <  1:698295/80‑1 (MQ=255)                  aGGTTACTCATCTTGCCGCCTTCAACAATCACTCCGCGCCGATGATGGAAGTCGTAATACCATGTTTCATCAGGATTTTcc                                                         <  2:698295/81‑1 (MQ=255)                   ggTTACTCATCTTGCCGCCTTCAACAATCACTCCGCGCCGATGATGGAAGTCGTAATACCATGTTTCATCAGGATTTTCCg                                                        <  2:698296/81‑1 (MQ=255)                    gTTACTCATCTTGCCGCCTTCAACAATCACTCCGCGCCGATGATGGAAGTCGTAATACCATGTTTCATCAGGATTTTCCgg                                                       >  2:698349/1‑81 (MQ=255)                       aCTCATCTTGCCGCCTTCAACAATCACTCCGCGCCGATGATGGAAGTCGTAATACCATGTTTCATCAGGATTTTCCGGGcc                                                    >  1:698372/1‑81 (MQ=255)                        cTCATCTTGCCGCCTTCAACAATCACTCCGCGCCGATGATGGAAGTCGTAATACCATGTTTCATCAGGATTTTCCGGGcc                                                    <  1:698298/80‑1 (MQ=255)                           aTCTTGCCGCCTTCAACAATCACTCCGCGCCGATGATGGAAGTCGTAATACCATGTTTCATCAGGATTTTCCGGGCCGTgg                                                <  1:698299/81‑1 (MQ=255)                             cTTGCCGCCTTCAACAATCACTCCGCGCCGATGATGGAAGTCGTAATACCATGTTTCATCAGGATTTTCCGGGCCGTGGAt                                              >  2:698360/1‑81 (MQ=255)                               tGCCGCCTTCAACAATCACTCCGCGCCGATGATGGAAGTCGTAATACCATGTTTCATCAGGATTTTCCGGGCCGTGGATg                                             >  1:698347/1‑80 (MQ=255)                                gccgccTTCAACAATCGCTCCGCGCCGATGATGGAAGTCGTAATACCATGTTTCATCAGGATTTTCCTGGCCGTGGATg                                             <  2:698300/79‑1 (MQ=255)                                gccgccTTCAACAATCACTCCGCGCCGATGATGGAAGTCGTAATACCATGTTTCATCAGGATTTTCCGGGCCGTGGAt                                              <  2:698301/78‑1 (MQ=255)                                gccgccTTCAACAATCACTCCGCGCCGATGATGGAAGTCGTAATACCATGTTTCATCAGGATTTTCCGGGCCGTGGATg                                             <  1:698300/79‑1 (MQ=255)                                 ccgccTTCAACAATCACTCCGCGCCGATGATGGAAGTCGTAATACCATGTTTCATCAGGATTTTCCGGGCCGTGGATGaa                                           <  2:698302/80‑1 (MQ=255)                                  cgccTTCAACAATCACTCCGCGCCGATGATGGAAGTCGTAATACCATGTTTCATCAGGATTTTCCGGGCCGTGGATGaaaa                                         >  2:698364/1‑81 (MQ=255)                                   gccTTCAACAATCACTCCGCGCCGATGATGGAAGTCGTAATACCATGTTTCATCAGGATTTTCCGGGCCGTGGATGaaaa                                         >  2:698354/1‑80 (MQ=255)                                   gccTTCAACAATCACTCCGCGCCGATGATGGAAGTCGTAATACCATGTTTCATCAGGATTTTCCGGGCCGTGGATGaaaa                                         >  1:698360/1‑80 (MQ=255)                                       tCAACAATCACTCCGCGCCGATGATGGAAGTCGTAATACCATGTTTCATCAGGATTTTCCGGGCCGTGGATGAAAACatat                                    >  1:698373/1‑81 (MQ=255)                                        caacaaTCACTCCGCGCCGATGATGGAAGTCGTAATACCATGTTTCATCAGGATTTTCCGGGCCGTGGATGAAAACata                                     <  2:698303/79‑1 (MQ=255)                                        caacaaTCACTCCGCGCCGATGATGGAAGTCGTAATACCATGTTTCATCAGGATTTTCCGGGCCGTGGATGAAAACATAtt                                   >  2:698355/1‑81 (MQ=255)                                            aaTCACTCCGCGCCGATGATGGAAGTCGTAATACCATGTTTCATCAGGATTTTCCGGGCCGTGGATGAAAACATATTtctc                               >  2:698353/1‑81 (MQ=255)                                             aTCACTCCGCGCCGATGATGGAAGTCGTAATACCATGTTTCATCAGGATTTTCCGGGCCGTGGATGAAAACATATTTctct                              <  1:698305/81‑1 (MQ=255)                                               cACTCCGCGCCGATGATGGAAGTCGTAATACCATGTTTCATCAGGATTTTCCGGGCCGTGGATGAAAACATATTTCTCTGa                            >  1:698366/1‑81 (MQ=255)                                                aCTCCGCGCCGATGATGGAAGTCGTAATACCATGTTTCATCAGGATTTTCCGGGCCGTGGATGAAAACATATTTCTCTGAc                           <  2:698305/81‑1 (MQ=255)                                                 cTCCGCGCCGATGATGGAAGTCGTAATACCATGTTTCATCAGGATTTTCCGGGCCGTGGATGAAAACATATTTCTCTGACt                          >  1:698343/1‑81 (MQ=255)                                                      cgcCGATGATGGAAGTCGTAATACCATGTTTCATCAGGATTTTCCGGGCCGTGGATGAAAACATATTTCTCTGACTTCGGa                     <  1:698307/81‑1 (MQ=255)                                                      cgcCGATGATGGAAGTCGTAATACCATGTTTCATCAGGATTTTCCGGGCCGTGGATGAAAACATATTTCTCTGACTTCGGa                     <  1:698308/81‑1 (MQ=255)                                                       gcCGATGATGGAAGTCGTAATACCATGTTTCATCAGGATTTTCCGGGCCGTGGATGAAAACATATTTCTCTGACTTCGGAt                    >  2:698363/1‑81 (MQ=255)                                                       gcCGATGATGGAAGTCGTAATACCATGTTTCATCAGGATTTTCCGGGCCGTGGATGAAAACATATTTCTCTGACTTCGGAt                    >  2:698386/1‑81 (MQ=255)                                                        ccGATGATGGAAGTCATAATACCATGTTTCATCAGGATTTTCCGGGCCGTGGATGAAAACATATTTCTCTGACTTCGGATg                   <  2:698308/81‑1 (MQ=255)                                                          gatgatGGAAGTCGTAATACCATGTTTCATCAGGATTTTCCGGGCCGTGGATGAAAACATATTTCTCTGACTTCGGATGaa                 >  1:698370/1‑81 (MQ=255)                                                             gatgGAAGTCGTAATACCATGTTTCACCAGGATTTTCCGGGCCGTGGATGAAAACATATTTCTCTGACTTCGGATGAACgg              >  1:698380/1‑81 (MQ=255)                                                               tgGAAGTCGTAATACCATGTTTCATCAGGATTTTCCGGGCCGTGGATGAAAACATATTTCTCTGACTTCGGATGAACGGt             >  2:698377/1‑80 (MQ=255)                                                                ggAAGTCGTAATACCATGTTTCATCAGGATTTTCCGGGCCGTGGATGAAAACATATTTCTCTGACTTCGGATGAACGGTca           >  2:698356/1‑81 (MQ=255)                                                                ggAAGTCGTAATACCATGTTTCATCAGGATTTTCCGGGCCGTGGATGAAAACATATTTCTCTGACTTCGGATGAACGGTca           >  1:698358/1‑81 (MQ=255)                                                                   aGTCGTAATACCATGTTTCATCAGGATTTTCCGGGCCGTGGATGAAAACATATTTCTCTGACTTCGGATGAACGGTcacca        <  1:698310/81‑1 (MQ=255)                                                                    gTCGTAATACCATGTTTCATCAGGATTTTCCGGGCCGTGGATGAAAACATATTTCTCTGACTTCGGATGAACGGTcaccac       <  1:698311/81‑1 (MQ=255)                                                                     tCGTAATACCATGTTTCATCAGGATTTTCCGGGCCGTGGATGAAAACATATTTCTCTGACTTCGGATGAACGGTCACCACg      <  1:698312/81‑1 (MQ=255)                                                                       gTAATACCATGTTTCATCAGGATTTTCCGGGCCGTGGATGAAAACATATTTCTCTGACTTCGGATGAACGGTCACCACGc     <  1:698313/80‑1 (MQ=255)                                                                        tAATACCATGTTTCATCAGGATTTTCCGGGCCGTGGATGAAAACATATTTCTCTGACTTCGGATGAACGGTCACCACGcc    >  1:698352/1‑80 (MQ=255)                                                                         aaTACCATGTTTCATCAGGATTTTCCGGGCCGTGGATGAAAACATATTTCTCTGACTTCGGATGAACGGTCACCACGCCGa  >  1:698375/1‑81 (MQ=255)                                                                            |                                                                              TAATATCCATCGCATCAAGGTTACTCATCTTGCCGCCTTCAACAATCACTCCGCGCCGATGATGGAAGTCGTAATGCCATGTTTCATCAGGATTTTCCGGGCCGTGGATGAAAACATATTTCTCTGACTTCGGATGAACGGTCACCACGCCGA  >  NC_000913/3868934‑3869086

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑