Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,876,270	A→G	I228T (ATT→ACT)	yidA ←	sugar phosphatase YidA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,876,270	0	A	G	100.0%	89.4 / NA	26	I228T (ATT→ACT)	yidA	sugar phosphatase YidA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (10/16);  total (10/16)

TTTGGTGACAAAGTTCGCCACTTCTTTCACTGAAGGAATAGCGTTATCCATCGCCACACCGACGCCTGCATATTCAATCATTGCGATATCGTTTTCCTGATCGCCAATCGCCATGATTTCTTCCGGTTTAATACCTAACACGTCGGCCAGT  >  NC_000913/3876194‑3876344                                                                             |                                                                           tttGGTGACAAAGTTCGCCACTTCTTTCACTGAAGGAATAGCGTTATCCATCGCCACACCGACGCCTGCATATTCAGTCAt                                                                        <  1:700300/81‑1 (MQ=255)     gTGACAAAGTTCGCCACTTCTTTCACTGAAGGAATAGCGTTATCCATCGCCACACCGACGCCTGCATATTCAGTCATTGc                                                                     <  1:700303/80‑1 (MQ=255)     gTGACAAAGTTCGCCACTTCTTTCACTGAAGGAATAGCGTTATCCATCGCCACACCGACGCCTGCATATTCAGTCATTGc                                                                     <  2:700300/80‑1 (MQ=255)     gTGACAAAGTTCGCCACTTCTTTCACTGAAGGAATAGCGTTATCCATCGCCACACCGACGCCTGCATATTCAGTCATTGCg                                                                    <  2:700301/81‑1 (MQ=255)           aaGTTCGCCACTTCTTTCACTGAAGGAATAGCGTTATCCATCGCCACACCGACGCCTGCATATTCAGTCATTGCGATATCg                                                              >  1:700345/1‑81 (MQ=255)            aGTTCGCCACTTCTTTCACTGAAGGAATAGCGTTATCCATCGCCACACCGACGCCTGCATATTCAGTCATTGCGATATCGt                                                             >  1:700365/1‑81 (MQ=255)                 gCCACTTCTTTCACTGAAGGAATAGCGTTATCCATCGCCACACCGACGCCTGCATATTCAGTCATTGCGATATCGTTTTcc                                                        >  1:700348/1‑81 (MQ=255)                   cACTTCTTTCACTGAAGGAATAGCGTTATCCATCGCCACACCGACGCCTGCATATTCAGTCATTGCGATATCGTTTTCCt                                                       >  1:700356/1‑80 (MQ=255)                        cttTCACTGAAGGAATAGCGTTATCCATCGCCACACCGACGCCTGCATATTCAGTCATTGCGATATCGTTTTCCTGatc                                                   <  1:700304/79‑1 (MQ=255)                             aCTGAAGGAATAGCGTTATCCATCGCCACACCGACGCCTGCATATTCAGTCATTGCGATATCGTTTTCCTGatcgccaatc                                            <  1:700305/81‑1 (MQ=255)                             aCTGAAGGAATAGCGTTATCCATCGCCACACCGACGCCTGCATATTCAGTCATTGCGATATCGTTTTCCTGatcgccaatc                                            >  2:700356/1‑81 (MQ=255)                                  aGGAATAGCGTTATCCATCGCCACACCGACGCCTGCATATTCAGTCATTGCGATATCGTTTTCCTGATCGCCAATCGCCAt                                       <  1:700306/81‑1 (MQ=255)                                         gCGTTATCCATCGCCACACCGACGCCTGCATATTCAGTCATTGCGATATCGTTTTCCTGATCGCCAATCGCCATGATttct                                <  1:700307/81‑1 (MQ=255)                                           gTTATCCATCGCCACACCGACGCCTGCATATTCAGTCATTGCGATATCGTTTTCCTGATCGCCAATCGCCATGATttcttc                              >  1:700364/1‑81 (MQ=255)                                            ttATCCATCGCCACACCGACGCCTGCATATTCAGTCATTGCGATATCGTTTTCCTGATCGCCAATCGCCATGATTTCTTcc                             <  1:700308/81‑1 (MQ=255)                                             tATTCATCGCCACACCGATGTCTGCATATTCAGTCATTGCGATATCGTTTTCCTGATCGCCAATCGTCATGATTTCTTCCg                            >  2:700351/1‑81 (MQ=255)                                                ccATCGCCACACCGACGCCTGCATATTCAGTCATTGCGATATCGTTTTCCTGATCGCCAATCGCCATGATTTCTTCCGGtt                         >  2:700358/1‑81 (MQ=255)                                                 cATCGCCACACCGACGCCTGCATATTCAGTCATTGCGATATCGTTTTCCTGATCGCCAATCGCCATGATTTCTTCCGGttt                        <  2:700308/81‑1 (MQ=255)                                                 cATCGCCACACCGACGCCTGCATATTCAGTCATTGCGATATCGTTTTCCTGATCGCCAATCGCCATGATTTCTTCCGGtt                         <  2:700309/80‑1 (MQ=255)                                                     gCCACACCGACGCCTGCATATTCAGTCATTGCGATATCGTTTTCCTGATCGCCAATCGCCATGATTTCTTCCGGTTTAATa                    >  2:700361/1‑81 (MQ=255)                                                        acacCGACGCCTGCATATTCAGTCATTGCGATATCGTTTTCCTGATCGCCAATCGCCATGATTTCTTCCGGTTTAATACCt                 <  2:700310/81‑1 (MQ=255)                                                                gCCTGCATATTCAGTCATTGCGATATCGTTTTCCTGATCGCCAATCGCCATGATTTCTTCCGGTTTAATACCTAACACGTc         <  1:700312/81‑1 (MQ=255)                                                                gCCTGCATATTCAGTCATTGCGATATCGTTTTCCTGATCGCCAATCGCCATGATTTCTTCCGGTTTAATACCTAACACGTc         <  2:700312/81‑1 (MQ=255)                                                                     cATATTCAGTCATTGCGATATCGTTTTCCTGATCGCCAATCGCCATGATTTCTTCCGGTTTAATACCTAACACGTCGGcc     <  1:700314/80‑1 (MQ=255)                                                                      atatTCAGTCATTGCGATATCGTTTTCCTGATCGCCAATCGCCATGATTTCTTCCGGTTTAATACCTAACACGTCGGCCAg   <  1:700315/81‑1 (MQ=255)                                                                        atTCAGTCATTGCGATATCGTTTTCCTGATCGCCAATCGCCATGATTTCTTCCGGTTTAATACCTAACACGTCGGCCAGt  >  2:700373/1‑80 (MQ=255)                                                                             |                                                                           TTTGGTGACAAAGTTCGCCACTTCTTTCACTGAAGGAATAGCGTTATCCATCGCCACACCGACGCCTGCATATTCAATCATTGCGATATCGTTTTCCTGATCGCCAATCGCCATGATTTCTTCCGGTTTAATACCTAACACGTCGGCCAGT  >  NC_000913/3876194‑3876344

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑