Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,883,138	C→G	V198L (GTG→CTG)	dnaA ←	chromosomal replication initiator protein DnaA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,883,138	0	C	G	100.0%	151.8 / NA	43	V198L (GTG→CTG)	dnaA	chromosomal replication initiator protein DnaA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (17/26);  total (17/26)

TAAACTCTTCGATCGCGTTGTTTTGCAGGGCTTTAACCATGTCCTGAACAAAGCGCTCGGAGTGCATATAAACCACTTTGGCATTCGGCTTGCGCGCCATAATGCCGTTACCCACCGCATGCAGCAGGTGAGTTTTACCCAGACCCGTGCCG  >  NC_000913/3883063‑3883214                                                                            |                                                                             tAAACTCTTCGATCGCGTTGTTTTGCAGGGCTTTAACCATGTCCTGAACAAAGCGCTCGGAGTGCATATAAACCAGTTTgg                                                                         >  1:702353/1‑81 (MQ=255)  aaaCTCTTCGATCGCGTTGTTTTGCAGGGCTTTAACCATGTCCTGAACAAAGCGCTCGGAGTGCATATAAACCAGTTTgg                                                                         <  1:702300/80‑1 (MQ=255)  aaaCTCTTCGATCGCGTTGTTTTGCAGGGCTTTAACCATGTCCTGAACAAAGCGCTCGGAGTGCATATAAACCAGTTTgg                                                                         <  1:702299/80‑1 (MQ=255)          cGATCGCGTTGTTTTGCAGGGCTTTAACCATGTCCTGAACAAAGCGCTCGGAGTGCATATAAACCAGTTTGGCATTCg                                                                   <  1:702301/78‑1 (MQ=255)          cGATCGCGTTGTTTTGCAGGGCTTTAACCATGTCCTGAACAAAGCGCTCGGAGTGCATATAAACCAGTTTGGCATTCGGc                                                                 <  2:702301/80‑1 (MQ=255)           gATCGCGTTGTTTTGCAGGGCTTTAACCATGTCCTGAACAAAGCGCTCGGAGTGCATATAAACCAGTTTGGCATTCGGCt                                                                <  1:702303/80‑1 (MQ=255)              cgcgTTGTTTTGCAGGGCTTTAACCATGTCCTGAACAAAGCGCTCGGAGTGCATATAAACCAGTTTGGCATTCGGCTTgcg                                                            >  1:702367/1‑81 (MQ=255)               gcgTTGTTTTGCAGGGCTTTAACCATGTCCTGAACAAAGCGCTCGGAGTGCATATAAACCAGTTTGGCATTCGGCTTgcgc                                                           >  2:702354/1‑81 (MQ=255)               gcgTTGTTTTGCAGGGCTTTAACCATGTCCTGAACAAAGCGCTCGGAGTGCATATAAACCAGTTTGGCATTCGGCTTgcgc                                                           <  1:702304/81‑1 (MQ=255)                 gttgttTTGCAGGGCTTTAACCATGTCCTGAACAAAGCGCTCGGAGTGCATATAAACCAGTTTGGCATTCGGCTTgcgcgc                                                         <  2:702304/81‑1 (MQ=255)                  ttgttTTGCAGGGCTTTAACCATGTCCTGAACAAAGCGCTCGGAGTGCATATAAACCAGTTTGGCATTCGGCTTgcgcgc                                                         >  2:702357/1‑80 (MQ=255)                     ttttGCAGGGCTTTAACCATGTCCTGAACAAAGCGCTCGGAGTGCATATAAACCAGTTTGGCATTCGGCTTGCGCGCCAt                                                      <  2:702305/80‑1 (MQ=255)                          cAGGGCTTTAACCATGTCCTGAACAAAGCGCTCGGAGTGCATATAAACCAGTTTGGCATTCGGCTTGCGCGCCATAATGcc                                                <  1:702307/81‑1 (MQ=255)                          cAGGGCTTTAACCATGTCCTGAACAAAGCGCTCGGAGTGCATATAAACCAGTTTGGCATTCGGCTTGCGCGCCATAATGc                                                 <  2:702307/80‑1 (MQ=255)                            gggCTTTAACCATGTCCTGAACAAAGCGCTCGGAGTGCATATAAACCAGTTTGGCATTCGGCTTGCGCGCCATAATGCCGt                                              <  2:702308/81‑1 (MQ=255)                              gCTTTAACCATGTCCTGAACAAAGCGCTCGGAGTGCATATAAACCAGTTTGGCATTCGGCTTGCGCGCCATAATGCCGTTa                                            >  1:702359/1‑81 (MQ=255)                               cTTTAACCATGTCCTGAACAAAGCGCTCGGAGTGCATATAAACCAGTTTGGCATTCGGCTTGCGCGCCATAATGCCGTTAc                                           <  2:702309/81‑1 (MQ=255)                                 ttAACCATGTCCTGAACAAAGCGCTCGGAGTGCATATAAACCAGTTTGGCATTCGGCTTGCGCGCCATAATGCCGTTAcc                                          >  2:702367/1‑80 (MQ=255)                                  tAACCATGTCCTGAACAAAGCGCTCGGAGTGCATATAAACCAGTTTGGCATTCGGCTTGCGCGCCATAATGCCGTTAccc                                         <  2:702310/80‑1 (MQ=255)                                  tAACCATGTCCTGAACAAAGCGCTCGGAGTGCATATAAACCAGTTTGGCATTCGGCTTGCGCGCCATAATGCCGTTAccc                                         <  1:702312/80‑1 (MQ=255)                                   aaCCATGTCCTGAACAAAGCGCTCGGAGTGCATATAAACCAGTTTGGCATTCGGCTTGCGCGCCATAATGCCGTTACCCa                                        <  1:702313/80‑1 (MQ=255)                                    aCCATGTCCTGAACAAAGCGCTCGGAGTGCATATAAACCAGTTTGGCATTCGGCTTGCGCGCCATAATGCCGTTACCCAcc                                      <  1:702314/81‑1 (MQ=255)                                     ccATGTCCTGAACAAAGCGCTCGGAGTGCATATAAACCAGTTTGGCATTCGGCTTGCGCGCCATAATGCCGTTACCCACCg                                     >  2:702370/1‑81 (MQ=255)                                      cATGTCCTGAACAAAGCGCTCGGAGTGCATATAAACCAGTTTGGCATTCGGCTTGCGCGCCATAATGCCGTTACCCACCg                                     >  2:702355/1‑80 (MQ=255)                                        tGTCCTGAACAAAGCGCTCGGAGTGCATATAGACCAGTTTGGCATTCGGCTTGCGCGCCATAATGCCGTTACCCACCGCAt                                  <  2:702314/81‑1 (MQ=255)                                        tGTCCTGAACAAAGCGCTCGGAGTGCATATAAACCAGTTTGGCATTCGGCTTGCGCGCCATAATGCCGTTACCCACCGCAt                                  >  1:702365/1‑81 (MQ=255)                                        tGTCCTGAACAAAGCGCTCGGAGTGCATATAAACCAGTTTGGCATTCGGCTTGCGCGCCATAATGCCGTTACCCACCGCAt                                  <  2:702315/81‑1 (MQ=255)                                        tGTCCTGAACAAAGCGCTCGGAGTGCATATAAACCAGGTTGGCAGGCGGCTTGCGCGCCAGAATGCCGTGACCCACCGCag                                  <  2:894449/81‑2 (MQ=255)                                              gAACAAAGCGCTCGGAGTGCATATAAACCAGTTTGGCATTCGGCTTGCGCGCCATAATGCCGTTACCCACCGCATgcag                              >  2:702360/1‑79 (MQ=255)                                               aaCAAAGCGCTCGGAGTGCATATAAACCAGTTTGGCATTCGGCTTGCGCGCCATAATGCCGTTACCCACCGCATgcagc                             <  1:702317/79‑1 (MQ=255)                                                       gcTCGGAGTGCATATAAACCAGTTTGGCATTCGGCTTGCGCGCCATAATGCCGTTACCCACCGCATGCAGCAGGTGAGttt                   >  2:702386/1‑81 (MQ=255)                                                        cTCGGAGTGCATATAAACCAGTTTGGCATTCGGCTTGCGCGCCATAATGCCGTTACCCACCGCATGCAGCAGGTGAGttt                   >  1:702370/1‑80 (MQ=255)                                                        cTCGGAGTGCATATAAACCAGTTTGGCATTCAGCTTGCGCGCCATAATGCCGTTACCCACCGCATGCAGCAGGTGAGtttt                  <  1:702318/81‑1 (MQ=255)                                                          cGGAGTGCATATAAACCAGTTTGGCATTCGGCTTGCGCGCCATAATGCCGTTACCCACCGCATGCAGCAGGTGAGTTTTAc                >  1:702363/1‑81 (MQ=255)                                                            gAGTGCATATAAACCAGTTTGGCATTCGGCTTGCGCGCCATAATGCCGTTACCCACCGCATGCAGCAGGTGAGTTTTAcc               >  1:702364/1‑80 (MQ=255)                                                               tGCATATAAACCAGTTTGGCATTCGGCTTGCGCGCCATAATGCCGTTACCCACCGCATGCAGCAGGTGAGTTTTACCCAGa           <  1:702319/81‑1 (MQ=255)                                                                  atatAAACCAGTTTGGCATTCGGCTTGCGCGCCATAATGCCGTTACCCACCGCATGCAGCAGGTGAGTTTTACCCAGAccc        <  1:702320/81‑1 (MQ=255)                                                                  atatAAACCAGTTTGGCATTCGGCTTGCGCGCCATAATGCCGTTACCCACCGCATGCAGCAGGTGAGTTTTACCCAGAccc        >  2:702378/1‑81 (MQ=255)                                                                     taAACCAGTTTGGCATTCGGCTTGCGCGCCATAATGCCGTTACCCACCGCATGCAGCAGGTGAGTTTTACCCAGACCCGTg     >  2:702356/1‑81 (MQ=255)                                                                     taAACCAGTTTGGCATTCGGCTTGCGCGCCATAATGCCGTTACCCACCGCATGCAGCAGGTGAGTTTTACCCAGACCCGTg     >  2:702387/1‑81 (MQ=255)                                                                       aaCCAGTTTGGCATTCGGCTTGCGCGCCATAATGCCGTTACCCACCGCATGCAGCAGGTGAGTTTTACCCAGACCCGTgcc   <  1:702321/81‑1 (MQ=255)                                                                        aCCAGTTTGGCATTCGGCTTGCGCGCCATAATGCCGTTACCCACCGCATGCAGCAGGTGAGTTTTACCCAGACCCGTgccg  <  1:702322/81‑1 (MQ=255)                                                                        aCCAGTTTGGCATTCGGCTTGCGCGCCATAATGCCGTTACCCACCGCATGCAGCAGGTGAGTTTTACCCAGACCCGTgccg  <  1:702323/81‑1 (MQ=255)                                                                            |                                                                             TAAACTCTTCGATCGCGTTGTTTTGCAGGGCTTTAACCATGTCCTGAACAAAGCGCTCGGAGTGCATATAAACCACTTTGGCATTCGGCTTGCGCGCCATAATGCCGTTACCCACCGCATGCAGCAGGTGAGTTTTACCCAGACCCGTGCCG  >  NC_000913/3883063‑3883214

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑