Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,900,470	C→T	V36V (GTG→GTA)	yieL ←	putative hydrolase YieL

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,900,470	0	C	T	100.0%	89.1 / NA	26	V36V (GTG→GTA)	yieL	putative hydrolase YieL
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (19/7);  total (19/7)

TTTTTGCCCCAGGGGCAAAGTAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCT  >  NC_000913/3900400‑3900538                                                                       |                                                                     tttttGCCCCAGGGGCAAAGTAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTTACCGGGATAg                                                            >  2:707061/1‑81 (MQ=255)      gCCCCAGGGGCAAAGTAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTTACCGGGATAGTGGGt                                                       >  1:707053/1‑81 (MQ=255)       ccccAGGGGCAAAGTAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTTACCGGGATAGTGGGTg                                                      >  2:707026/1‑81 (MQ=255)           aGGGGCAAAGTAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTTACCGGGATAGTGGGTGCCgg                                                  >  2:707049/1‑81 (MQ=255)           aGGGGCAAAGTAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTTACCGGGATAGTGGGTGCCgg                                                  >  2:707048/1‑81 (MQ=255)              ggCAAAGTAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTTACCGGGATAGTGGGTGCCGggct                                               >  2:707045/1‑81 (MQ=255)                 aaaGTAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTTACCGGGATAGTGGGTGCCGggctggc                                            >  2:707051/1‑81 (MQ=255)                 aaaGTAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTTACCGGGATAGTGGGTGCCGggctggc                                            >  1:707051/1‑81 (MQ=255)                 aaaGTAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTTACCGGGATAGTGGGTGCCGggctggc                                            >  2:707038/1‑81 (MQ=255)                  aaGTAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTTACCGGGATAGTGGGTGCCGggctggct                                           >  2:707040/1‑81 (MQ=255)                         gAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTTACCGGGATAGTGGGTGCCGGGCTGGCTGGCatat                                    <  1:707005/81‑1 (MQ=255)                           aaGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTTACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATcc                                  >  1:707017/1‑81 (MQ=255)                           aaGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTTACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATcc                                  <  2:707005/81‑1 (MQ=255)                             ggTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTTACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCgc                                <  2:707006/81‑1 (MQ=255)                               tCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTTACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCgctg                              >  2:707035/1‑81 (MQ=255)                                 aCGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTTACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCgctgc                             <  1:707008/80‑1 (MQ=255)                                  cGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTTACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTa                           >  1:707057/1‑81 (MQ=255)                                    cTGTTATCGGCATTGACCTGAGTCACATACTGTTTTACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATg                         <  2:707008/81‑1 (MQ=255)                                           cGGCATTGACCTGAGTCACATACTGTTTTACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACt                  >  2:707039/1‑81 (MQ=255)                                            ggCATTTACCTGAGTCACATACTGTTTTACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACt                  >  1:707054/1‑80 (MQ=255)                                                   aCCTGAGTCACATACTGTTTTACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCata           >  2:707046/1‑78 (MQ=255)                                                   aCCTGAGTCACATACTGTTTTACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGa           <  1:707010/80‑1 (MQ=255)                                                     cTGAGTCACATACTGTTTTACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGaaa         >  2:707044/1‑80 (MQ=255)                                                        aGTCACATACTGTTTTACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATAcc     >  2:707058/1‑81 (MQ=255)                                                         gTCACATACTGTTTTACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCg    >  1:707038/1‑81 (MQ=255)                                                            acaTACTGTTTTACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCt  <  1:707011/80‑1 (MQ=255)                                                                       |                                                                     TTTTTGCCCCAGGGGCAAAGTAGCGAAAGGTCACGCTGTTATCGGCATTGACCTGAGTCACATACTGTTTCACCGGGATAGTGGGTGCCGGGCTGGCTGGCATATCCGCTGCTATGGCCCACTGAGCAGAAATACCGCT  >  NC_000913/3900400‑3900538

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑