Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	389,984	A→G	intergenic (‑257/‑267)	hemB ← / → yaiT	porphobilinogen synthase/putative autotransporter YaiT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	389,984	0	A	G	100.0%	38.0 / NA	12	intergenic (‑257/‑267)	hemB/yaiT	porphobilinogen synthase/putative autotransporter YaiT
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (6/6);  total (6/6)

TGGTTATTATTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGATATTTATCTGGTTTGTGTTTGTGATATTTATTATGCGTATGCTTCAAAAACAAAATTATCTG  >  NC_000913/389911‑390046                                                                          |                                                               tGGTTATTATTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGGTATTTAt                                                         >  1:79993/1‑81 (MQ=255)       ttattTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGGTATTTATCTGgt                                                    <  1:79977/80‑1 (MQ=255)              ttGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGGTATTTATCTGgtttgtgttt                                            >  1:79992/1‑81 (MQ=255)                    tttGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGGTATTTATCTGGTTTGTGTTTGTGata                                      >  1:79994/1‑81 (MQ=255)                      tGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGGTATTTATCTGGTTTGTGTTTGTGAtttt                                    >  1:79998/1‑81 (MQ=255)                              ctgctATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGGTATTTATCTGGTTTGTGTTTGTGATATTTATTATGc                            <  1:79978/81‑1 (MQ=255)                                    taAATTGAATAATTAAAACTTTGTTGCATATTCATAGGGTATTTATCTGGTTTGTGTTTGTGATATTTATTATGCGTATg                       <  1:79979/80‑1 (MQ=255)                                    taAATTGAATAATTAAAACTTTGTTGCATATTCATAGGGTATTTATCTGGTTTGTGTTTGTGATATTTATTATGCGTATg                       <  1:79980/80‑1 (MQ=255)                                              aatTAAAACTTTGTTGCATATTCATAGGGTATTTATCTGGTTTGTGTTTGTGATATTTATTATGCGTATGCTTCAAAAAc             >  2:79991/1‑80 (MQ=255)                                               atTAAAACTTTGTTGCATATTCATAGGGTATTTATCTGGTTTGTGTTTGTGATATTTATTATGCGTATGCTTCAAAAACa            <  2:79981/80‑1 (MQ=255)                                                ttAAAACTTTGTTGCATATTCATAGGGTATTTATCTGGTTTGTGTTTGTGATATTTATTATGCGTATGCTTCAAAAACaaa          <  2:79982/81‑1 (MQ=255)                                                        ttgttgCATATTCATAGGGTATTTATCTGGTTTGTGTTTGTGATATTTATTATGCGTATGCTTCAAAAACAAAATTATCTg  >  1:79995/1‑81 (MQ=255)                                                                          |                                                               TGGTTATTATTTTTTGGAGTTTGGTTGCGCTGCTATAAATTGAATAATTAAAACTTTGTTGCATATTCATAGGATATTTATCTGGTTTGTGTTTGTGATATTTATTATGCGTATGCTTCAAAAACAAAATTATCTG  >  NC_000913/389911‑390046

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑