Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,983,380	C→A	R141R (CGC→CGA)	aslB →	putative anaerobic sulfatase maturation enzyme AslB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,983,380	0	C	A	100.0%	143.5 / NA	41	R141R (CGC→CGA)	aslB	putative anaerobic sulfatase maturation enzyme AslB
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (22/19);  total (22/19)

TGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGTGACTAAAGGTGGCAGACCCACGCATAAGCTGGTGATGCGTGCCCTGACGCTCCTGCAAAAACATCATGTCGAC  >  NC_000913/3983303‑3983455                                                                              |                                                                            tGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGAGTg                                                                          <  1:729780/81‑1 (MQ=255)   cgcgTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGAGTGAc                                                                        <  2:729780/81‑1 (MQ=255)   cgcgTTTCTAGCGGAGCATCATTTTATTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGAGTGAc                                                                        >  2:729830/1‑81 (MQ=255)      gTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGAGTGACTaa                                                                     <  2:729781/81‑1 (MQ=255)      gTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGAGTGACTa                                                                      >  2:729844/1‑80 (MQ=255)       tttCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGAGTGACTa                                                                      >  1:729856/1‑79 (MQ=255)             gcgGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGAGTGACTAAAGGTgg                                                               <  2:729782/80‑1 (MQ=255)             gcgGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGAGTGACTAAAGGTGGc                                                              <  1:729784/81‑1 (MQ=255)               ggAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGAGTGACTAAAGGTgg                                                               >  1:729865/1‑78 (MQ=255)                     tcatTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGAGTGACTAAAGGTGGCAGACCCAc                                                      >  1:729844/1‑81 (MQ=255)                     tcatTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGAGTGACTAAAGGTGGCAGACCCAc                                                      <  1:729785/81‑1 (MQ=255)                      catTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGAGTGACTAAAGGTGGCAGACCCACg                                                     >  1:729824/1‑81 (MQ=255)                       atTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGAGTGACTAAAGGTGGCAGACCCACg                                                     >  1:729830/1‑80 (MQ=255)                       atTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGAGTGACTAAAGGTGGCAGACCCACg                                                     <  2:729785/80‑1 (MQ=255)                          ttCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGAGTGACTAAAGGTGGCAGACCCACGCAt                                                  <  1:729787/80‑1 (MQ=255)                          ttCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGAGTGACTAAAGGTGGCAGACCCACGCAt                                                  >  1:729853/1‑80 (MQ=255)                               gttgGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGAGTGACTAAAGGTGGCAGACCCACGCATAAGCt                                             <  1:729788/80‑1 (MQ=255)                               gttgGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGAGTGACTAAAGGTGGCAGACCCACGCATAAGCt                                             <  2:729788/80‑1 (MQ=255)                                   gTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGAGTGACTAAAGGTGGCAGACCCACGCATAAGCTGGTGa                                        >  2:729838/1‑81 (MQ=255)                                   gTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGAGTGACTAAAGGTGGCAGACCCACGCATAAGCTGGTGa                                        >  1:729850/1‑81 (MQ=255)                                    tttATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGAGTGACTAAAGGTGGCAGACCCACGCATAAGCTGGTGAt                                       >  1:729860/1‑81 (MQ=255)                                    tttATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGAGTGACTAAAGGTGGCAGACCCACGCATAAGCTGGTGAt                                       >  2:729854/1‑81 (MQ=255)                                       aTCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGAGTGACTAAAGGTGGCAGACCCACGCATAAGCTGGTGATGCg                                    >  1:729846/1‑81 (MQ=255)                                        tCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGAGTGACTAAAGGTGGCAGACCCACGCATAAGCTGGTGATGCGt                                   <  1:729790/81‑1 (MQ=255)                                             ggATGGCCCGCCTGAGATCCACAATCAATATCGAGTGACTAAAGGTGGCAGACCCACGCATAAGCTGGTGATGCGTGCCCt                              >  2:729857/1‑81 (MQ=255)                                              gATGGCCCGCCTGAGATCCACAATCAATATCGAGTGACTAAAGGTGGCAGACCCACGCATAAGCTGGTGATGCGTGccc                               <  1:729791/79‑1 (MQ=255)                                                tGGCCCGCCTGAGATCCACAATCAATATCGAGTGACTAAAGGTGGCAGACCCACGCATAAGCTGGTGATGCGTGCCCTGAc                           <  2:729791/81‑1 (MQ=255)                                                tGGCCCGCCTGAGATCCACAATCAATATCGAGTGACTAAAGGTGGCAGACCCACGCATAAGCTGGTGATGCGTGCCCTGAc                           <  2:729792/81‑1 (MQ=255)                                                  gCCCGCCTGAGATCCACAATCAATATCGAGTGACTAAAGGTGGCAGACCCACGCATAAGCTGGTGATGCGTGCCCTGACGc                         >  2:729847/1‑81 (MQ=255)                                                    ccGCCTGAGATCCACAATCAATATCGAGTGACTAAAGGTGGCAGACCCACGCATAAGCTGGTGATGCGTGCCCTGACGCTc                       <  2:729793/81‑1 (MQ=255)                                                     cGCCTGAGATCCACAATCAATATCGAGTGACTAAAGGTGGCAGACCCACGCATAAGCTGGTGATGCGTGCCCTGACGCTcc                      >  1:729866/1‑81 (MQ=255)                                                          gagaTCCACAATCAATATCGAGTGACTAAAGGTGGCAGACCCACGCATAAGCTGGTGATGCGTGCCCTGACGCTCCTGCa                  >  1:729868/1‑80 (MQ=255)                                                           agaTCCACAATCAATATCGAGTGACTAAAGGTGGCAGACCCACGCATAAGCTGGTGATGCGTGCCCTGACGCTCCTGCaa                 >  2:729846/1‑80 (MQ=255)                                                            gagccACAATCAATATCGAGTGACTAAAGGTGGCAGACCCACGCATAAGCTGGTGATGCGTGCCCTGACGCTCCTGCaaa                >  2:729863/4‑80 (MQ=255)                                                                  caatcaatATCGAGTGACTAAAGGTGGCAGACCCACGCATAAGCTGGTGATGCGTGCCCTGACGCTCCTGCAAAAAcatca         <  1:729795/81‑1 (MQ=255)                                                                   aatcaatATCGAGTGACTAAAGGTGTCAGACCCACGCATAAGCTGGTGATGCGTGCCCTGACGCTCCTGCAAAAAcatcat        <  1:729796/81‑1 (MQ=255)                                                                   aatcaatATCGAGTGACTAAAGGTGGCAGACCCACGCATAAGCTGGTGATGCGTGCCCTGACGCTCCTGCAAAAAcatcat        <  2:729796/81‑1 (MQ=255)                                                                      caatATCGAGTGACTAAAGGTGGCAGACCCACGCATAAGCTGGTGATGCGTGCCCTGACGCTCCTGCAAAAACATCATGTc     >  1:729862/1‑81 (MQ=255)                                                                       aatATCGAGTGACTAAAGGTGGCAGACCCACGCATAAGCTGGTGATGCGTGCCCTGACGCTCCTGCAAAAACATCATGTc     >  1:729852/1‑80 (MQ=255)                                                                       aatATCGAGTGACTAAAGGTGGCAGACCCACGCATAAGCTGGTGATGCGTGCCCTGACGCTCCTGCAAAAACATCATGTc     >  1:729854/1‑80 (MQ=255)                                                                         tatCGAGTGACTAAAGGTGGCAGACCCACGCATAAGCTGGTGATGCGTGCCCTGACGCTCCTGCAAAAACATCATGTCGAc  <  2:729797/81‑1 (MQ=255)                                                                              |                                                                            TGCGCGTTTCTCGCGGAGCATCATTTTCTTGTTGGTTTATCGCTGGATGGCCCGCCTGAGATCCACAATCAATATCGCGTGACTAAAGGTGGCAGACCCACGCATAAGCTGGTGATGCGTGCCCTGACGCTCCTGCAAAAACATCATGTCGAC  >  NC_000913/3983303‑3983455

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑