Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,007,952	A→G	K66E (AAA→GAA)	rhtC →	L‑threonine exporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,007,952	0	A	G	100.0%	145.2 / NA	41	K66E (AAA→GAA)	rhtC	L‑threonine exporter
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (24/17);  total (24/17)

GTGCTGGGCATTACCTGCGGCGTAATGGTTTGGGCTGGGATTGCGCTGCTTGGCCTGCATTTGATTATCGAAAAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTGGCCTGTATCTCTGCTGGATGGGTTACCAGATGCTACGT  >  NC_000913/4007880‑4008029                                                                         |                                                                              gTGCTGGGCATTACCTGCGGCGTAATGGTTTGGGCTGGGATTGCGCTGCTTGGCCTGCATTTGATTATCGAAGAAATGGcc                                                                       >  1:736653/1‑81 (MQ=255)  tGCTGGGCATTACCTGCGGCGTAATGGTTTGGGCTGGGATTGCGCTGCTTGGCCTGCATTTGATTATCGAAGAAATGGCCt                                                                      <  1:736594/81‑1 (MQ=255)  tGCTGGGCATTACCTGCGGCGTAATGGTTTGGGCTGGGATTGCGCTGCTTGGCCTGCATTTGATTATCGAAGAAATGGCCt                                                                      <  2:736594/81‑1 (MQ=255)   gCTGGGCATTACCTGCGGCGTAATGGTTTGGGCTGGGATTGCGCTGCTTGGCCTGCATTTGATTATCGAAGAAATGGCCTg                                                                     >  1:736640/1‑81 (MQ=255)   gCTGGGCATTACCTGCGGCGTAATGGTTTGGGCTGGGATTGCGCTGCTTGGCCTGCATTTGATTATCGAAGAAATGGCCTg                                                                     >  1:736652/1‑81 (MQ=255)    cTGGGCATTACCTGCGGCGTAATGGTTTGGGCTGGGATTGCGCTGCTTGGCCTGCATTTGATTATCGAAGAAATGGCCTgg                                                                    >  1:736650/1‑81 (MQ=255)       ggCATTACCTGCGGCGTAATGGTTTGGGCTGGGATTGCGCTGCTTGGCCTGCATTTGATTATCGAAGAAATGGCCTGGCt                                                                  <  2:736595/80‑1 (MQ=255)          aTTACCTGCGGCGTAATGGTTTGGGCTGGGATTGCGCTGCTTGGCCTGCATTTGATTATCGAAGAAATGGCCTGGCTGCa                                                               >  2:736661/1‑80 (MQ=255)          aTTACCTGCGGCGTAATGGTTTGGGCTGGGATTGCGCTGCTTGGCCTGCATTTGATTATCGAAGAAATGGCCTGGCTGCa                                                               >  1:736647/1‑80 (MQ=255)           ttACCTGCGGCGTAATGGTTTGGGCTGGGATTGCGCTGCTTGGCCTGCATTTGATTATCGAAGAAATGGCCTGGCTGCATa                                                             <  2:736596/81‑1 (MQ=255)             aCCTGCGGCGTAATGGTTTGGGCTGGGATTGCGCTGCTTGGCCTGCATTTGATTATCGAAGAAATGGCCTGGCTGCATAc                                                            <  1:736598/80‑1 (MQ=255)             aCCTGCGGCGTAATGGTTTGGGCTGGGATTGCGCTGCTTGGCCTGCATTTGATTATCGAAGAAATGGCCTGGCTGCATAc                                                            <  2:736598/80‑1 (MQ=255)             aCCTGCGGCGTAATGGTTTGGGCTGGGATTGCGCTGCTTGGCCTGCATTTGATTATCGAAGAAATGGCCTGGCTGCATACg                                                           >  1:736638/1‑81 (MQ=255)                 gcggcgTAATGGTTTGGGCTGGGATTGCGCTGCTTGGCCTGCATTTGATTATCGAAGAAATGGCCTGGCTGCATACGCTGa                                                       >  1:736639/1‑81 (MQ=255)                   ggcgTAATGGTTTGGGCTGGGATTGCGCTGCTTGGCCTGCATTTGATTATCGAAGAAATGGCCTGGCTGCATACGCTGAtt                                                     <  1:736600/81‑1 (MQ=255)                    gcgTAATTGTTTGGGCTGGGATTGCGCTGCTTGGCCTGCATTTGATTATCGAAGAAATGGCCTGGCTGCATACGCTGATTa                                                    <  2:736600/81‑1 (MQ=255)                    gcgTAATGGTTTGGGCTGGGATTGCGCTGCTTGGCCTGCATTTGATTATCGAAGAAATGGCCTGGCTGCATACGCTGATTa                                                    >  1:736658/1‑81 (MQ=255)                      gTAATGGTTTGGGCTGGGATTGCGCTGCTTGGCCTGCATTTGATTATCGAAGAAATGGCCTGGCTGCATACGCTGATTAtg                                                  >  1:736649/1‑81 (MQ=255)                        aaTGGTTTGGGCTGGGATTGCGCTGCTTGGCCTGCATTTGATTATCGAAGAAATGGCCTGGCTGCATACGCTGATTAtggt                                                >  2:736660/1‑81 (MQ=255)                        aaTGGTTTGGGCTGGGATTGCGCTGCTTGGCCTGCATTTGATTATCGAAGAAATGGCCTGGCTGCATACGCTGATTAtggt                                                >  1:736659/1‑81 (MQ=255)                             tttGGGCTGGGATTGCGCTGCTTGGCCTGCATTTGATTATCGAAGAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCg                                           >  2:736645/1‑81 (MQ=255)                               tGGGCTGGGATTGCGCTGCTTGGCCTGCATTTGATTATCGAAGAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCgg                                          <  1:736603/80‑1 (MQ=255)                               tGGGCTGGGATTGCGCTGCTTGGCCTGCATTTGATTATCGAAGAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCgg                                          <  2:736601/80‑1 (MQ=255)                                 ggCTGGGATTGCGCTGCTTGGCCTGCATTTGATTATCGAAGAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTg                                        <  1:736604/80‑1 (MQ=255)                                  gCTGGGATTGCGCTGCTTGGCCTGCATTTGATTATCGAAGAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTGGc                                      >  2:736647/1‑81 (MQ=255)                                     gggATTGCGCTGCTTGGCCTGCATTTGATTATCGAAGAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTGGCCTg                                   >  2:736662/1‑81 (MQ=255)                                     gggATTGCGCTGCTTGGCCTGCATTTGATTATCGAAGAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTGGCCTg                                   >  2:736664/1‑81 (MQ=255)                                      ggATTGCGCTGCTTGGCCTGCATTTGATTATCGAAGAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTGGCCt                                    <  2:736604/79‑1 (MQ=255)                                       gATTGCGCTGCTTGGCCTGCATTTGATTATCGAAGAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTGGCCTGTa                                 >  2:736668/1‑81 (MQ=255)                                       gATTGCGCTGCTTGGCCTGCATTTGATTATCAAAGAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTGGCCTGTa                                 <  2:736605/81‑1 (MQ=255)                                         ttGCGCTGCTTGGCCTGCATTTGATTATCGAAGAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTGGCCTGTAtc                               <  2:736606/81‑1 (MQ=255)                                            cgcTGCTTGGCCTGCATTTGATTATCGAAGAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTGGCCTGTATctct                            >  2:736672/1‑81 (MQ=255)                                             gctgctTGGCCTGCATTTGATTATCGAAGAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTGGCCTGTATCTctg                           >  1:736672/1‑81 (MQ=255)                                                gctTGGCCTGCATTTGATTATCGAAGAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTGGCCTGTATCTctgctg                        >  2:736670/1‑81 (MQ=255)                                                 ctTGGCCTGCATTTGATTATCGAAGAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTGGCCTGTATCTctgctg                        <  2:736607/80‑1 (MQ=255)                                                 ctTGGCCTGCATTTGATTATCGAAGAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTGGCCTGTATCTctgctg                        <  1:736609/80‑1 (MQ=255)                                                  ttGGCCTGCATTTGATTATCGAAGAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTGGCCTGTATCTCTGCTGGa                      >  2:736663/1‑81 (MQ=255)                                                         gCATTTGATTATCGAAGAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTGGCCTGTATCTCTGCTGGATGGGTTa               >  1:736655/1‑81 (MQ=255)                                                           aTTTGATTATCGAAGAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTGGCCTGTATCTCTGCTGGATGGGTTAc              >  2:736681/1‑80 (MQ=255)                                                            tttGATTATCGAAGAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTGGCCTGTATCTCTGCTGGATGGGTTAc              >  2:736656/1‑79 (MQ=255)                                                                    tCGAAGAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTGGCCTGTATCTCTGCTGGATGGGTTACCAGATGCTa     <  2:736609/80‑1 (MQ=255)                                                                      gaagaaATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTGGCCTGTATCTCTGCTGGATGGGTTACCAGATGCTACgt  >  1:736668/1‑81 (MQ=255)                                                                      gaagaaATGGCCTAGCTGCATACGCTGATTATGGTGGGCGGTGGCCTGTATCTCTGCTGGATGGGTTACCAGATGCTACg   <  2:736610/80‑1 (MQ=255)                                                                         |                                                                              GTGCTGGGCATTACCTGCGGCGTAATGGTTTGGGCTGGGATTGCGCTGCTTGGCCTGCATTTGATTATCGAAAAAATGGCCTGGCTGCATACGCTGATTATGGTGGGCGGTGGCCTGTATCTCTGCTGGATGGGTTACCAGATGCTACGT  >  NC_000913/4007880‑4008029

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑