Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,090,946	G→A	A474V (GCC→GTC)	frvB ←	putative PTS enzyme IIBC component FrvB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,090,946	0	G	A	100.0%	184.4 / NA	51	A474V (GCC→GTC)	frvB	putative PTS enzyme IIBC component FrvB
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (26/25);  total (26/25)

GTTCATCGCCGCTGACGGCGCTGGCTTCGCACAACTGTTGCAGTAACTCAATGTTCATGGACAAGCTCCTGTTGCGCGGCTTTCGCTTTGCGGCGTAACCACAATCCTTTCAGGACGATAATGAATGAAATGTTCAGCGCGAGGCCAATCGCCAG  >  NC_000913/4090868‑4091022                                                                               |                                                                             gTTCATCGCCGCTGACGGCGCTGGCTTCGCACAACTGTTGCAGTAACTCAATGTTCATGGACAAGCTCCTGTTGCGCGACt                                                                            >  2:758885/1‑81 (MQ=255)   tCATCGCCGCTGACGGCGCTGGCTTCGCACAACTGTTGCAGTAACTCAATGTTCATGGACAAGCTCCTGTTGCGCGACttt                                                                          >  2:758888/1‑81 (MQ=255)   tCATCGCCGCTGACGGCGCTGGCTTCGCACAACTGTTGCAGTAACTCAATGTTCATGGACAAGCTCCTGTTGCGCGACttt                                                                          >  1:758892/1‑81 (MQ=255)    cATCGCCGCTGACGGCGCTGGCTTCGCACAACTGTTGCAGTAACTCAATGTTCATGGACAAGCTCCTGTTGCGCGACttt                                                                          <  1:758841/80‑1 (MQ=255)     aTCGCCGCTGACGGCGCTGGCTTCGCACAACTGTTGCAGTAACTCAATGTTCATGGACAAGCTCCTGTTGCGCGACTTTc                                                                         <  2:758841/80‑1 (MQ=255)      tCGCCGCTGACGGCGCTGGCTTCGCACAACTGTTGCAGTAACTCAATGTTCATGGACAAGCTCCTGTTGCGCGACTTTCGc                                                                       >  2:758889/1‑81 (MQ=255)        gccgcTGACGGCGCTGGCTTCGCACAACTGTTGCAGTAACTCAATGTTCATGGACAAGCTCCTGTTGCGCGACTTTCGCtt                                                                     >  2:758909/1‑81 (MQ=255)         ccgcTGACGGCGCTGGCTTCGCACAACTGTTGCAGTAACTCAATGTTCATGGACAAGCTCCTGTTGCGCGACTTTCGCttt                                                                    <  2:758842/81‑1 (MQ=255)           gcTGACGGCGCTGGCTTCGCACAACTGTTGCAGTAACTCAATGTTCATGGACAAGCTCCTGTTGCGCGACTTTCGCTTTgc                                                                  >  2:758907/1‑81 (MQ=255)           gcTGACGGCGCTGGCTTCGCACAACTGTTGCAGTAACTCAATGTTCATGGACAAGCTCCTGTTGCGCGACTTTCGCTTTgc                                                                  >  2:758895/1‑81 (MQ=255)               aCGGCGCTGGCTTCGCACAACTGTTTCAGTAACTCAATGTTCATGGACAAGCTCCTGTTGCGCGACTTTCGCTTTgcggc                                                               <  1:758844/80‑1 (MQ=255)               aCGGCGCTGGCTTCGCACAACTGTTGCAGTAACTCAATGTTCATGGACAAGCTCCTGTTGCGCGACTTTCGCTTTgcgg                                                                <  2:758844/79‑1 (MQ=255)                 ggCGCTGGCTTCGCACAACTGTTGCAGTAACTCAATGTTCATGGACAAGCTCCTGTTGCGCGACTTTCGCTTTGCGGCGt                                                             >  2:758883/1‑80 (MQ=255)                 ggCCCTGGCTTCGCACAACTGTTGCAGTAACTCAATGTTCATGGACAAGCTCCTGTTGCGCGACTTTCGCTTTGCGGCGt                                                             >  2:758881/1‑80 (MQ=255)                       ggCTTCGCACAACTGTTGCAGTAACTCAATGTTCATGGACAAGCTCCTGTTGCGCGACTTTCGCTTTGCGGCGTAACcac                                                       >  2:758887/1‑80 (MQ=255)                        gCTTCGCACAACTGTTGCAGTAACTCAATGTTCATGGACAAGCTCCTGTTGCGCGACTTTCGCTTTGCGGCGTAACcac                                                       <  1:758848/79‑1 (MQ=255)                        gCTTCGCACAACTGTTGCAGTAACTCAATGTTCATGGACAAGCTCCTGTTGCGCGACTTTCGCTTTGCGGCGTAACCACaa                                                     <  2:758845/81‑1 (MQ=255)                        gCTTCGCACAACTGTTGCAGTAACTCAATGTTCATGGACAAGCTCCTGTTGCGCGACTTTCGCTTTGCGGCGTAACCACaa                                                     <  2:758846/81‑1 (MQ=255)                           tCGCACAACTGTTGCAGTAACTCAATGTTCATGGACAAGCTCCTGTTGCGCGACTTTCGCTTTGCGGCGTAACCACAATcc                                                  >  2:758893/1‑81 (MQ=255)                            cGCACAACTGTTGCAGTAACTCAATGTTCATGGACAAGCTCCTGTTGCGCGACTTTCGCTTTGCGGCGTAACCACAATcc                                                  <  1:758849/80‑1 (MQ=255)                            cGCACAACTGTTGCAGTAACTCAATGTTCATGGACAAGCTCCTGTTGCGCGACTTTCGCTTTGCGGCGTAACCACAATc                                                   <  1:758851/79‑1 (MQ=255)                            cGCACAACTGTTGCAGTAACTCAATGTTCATGGACAAGCTCCTGTTGCGCGACTTTCGCTTTGCGGCGTAACCACAATc                                                   <  1:758850/79‑1 (MQ=255)                            cGCACAACTGTTGCAGTAACTCAATGTTCATGGACAAGCTCCTGTTGCGCGACTTTCGCTTTGCGGCGTAACCACAATCCt                                                 >  1:758904/1‑81 (MQ=255)                            cGCACAACTGTTGCAGTAACTCAATGTTCATGGACAAGCTCCTGTTGCGCGACTTTCGCTTTGCGGCGTAACCACAATCCt                                                 >  1:758898/1‑81 (MQ=255)                             gCACAACTGTTGCAGTAACTCAATGTTCATGGACAAGCTCCTGTTGCGCGACTTTCGCTTTGCGGCGTAACCACAATCCtt                                                >  1:758885/1‑81 (MQ=255)                             gCACAACTGTTGCAGTAACTCAATGTTCATGGACAAGCTCCTGTTGCGCGACTTTCGCTTTGCGGCGTAACCACAATCCtt                                                <  2:758851/81‑1 (MQ=255)                              cacaACTGTTGCAGTAACTCAATGTTCATGGACAAGCTCCTGTTGCGCGACTTTCGCTTTGCGGCGTAACCACAATCCttt                                               >  1:758913/1‑81 (MQ=255)                              cacaACTGTTGCAGTAACTCAATGTTCATGGACAAGCTCCTGTTGCGCGACTTTCGCTTTGCGGCGTAACCACAATCCttt                                               >  1:758911/1‑81 (MQ=255)                              cacaACTGTTGCAGTAACTCAATGTTCATGGACAAGCTCCTGTTGCGCGACTTTCGCTTTGCGGCGTAACCACAATCCttt                                               >  2:758894/1‑81 (MQ=255)                                 aaCTGTTGCAGTAACTCAATGTTCATGGACAAGCTCCTGTTGCGCGACTTTCGCTTTGCGGCGTAACCACAATCCTTTCAg                                            >  2:758886/1‑81 (MQ=255)                                  aCTGTTGCAGTAACTCAATGTTCATGGACAAGCTCCTGTTGCGCGACTTTCGCTTTGCGGCGTAACCACAATCCTTTCAgg                                           <  2:758852/81‑1 (MQ=255)                                  aCTGTTGCAGTAACTCAATGTTCATGGACAAGCTCCTGTTGCGCGACTTTCGCTTTGCGGCGTAACCACAATCCTTTCAgg                                           <  2:758853/81‑1 (MQ=255)                                   cTGTTGCAGTAACTCAATGTTCATGGACAAGCTCCTGTTGCGCGACTTTCGCTTTGCGGCGTAACCACAATCCTTTCAGGa                                          >  2:758899/1‑81 (MQ=255)                                     gTTGCAGTAACTCAATGTTCATGGACAAGCTCCTGTTGCGCGACTTTCGCTTTGCGGCGTAACCACAATCCTTTCAGGAc                                         >  2:758914/1‑80 (MQ=255)                                          aGTAACTCAATGTTCATGGACAAGCTCCTGTTGCGCGACTTTCGCTTTGCGGCGTAACCACAATCCTTTCAGGACGATaat                                   >  2:758892/1‑81 (MQ=255)                                                  aaTGTTCATGGACAAGCTCCTGTTGCGCGACTTTCGCTTTGCGGCGTAACCACAATCCTTTCAGGACGATAatgaatga                             <  1:758855/79‑1 (MQ=255)                                                  aaTGTTCATGGACAAGCTCCTGTTGCGCGACTTTCGCTTTGCGGCGTAACCACAATCCTTTCAGGACGATAAtgaatgaa                            <  1:758857/80‑1 (MQ=255)                                                  aaTGTTCATGGACAAGCTCCTGTTGCGCGACTTTCGCTTTGCGGCGTAACCACAATCCTTTCAGGACGATAATGAATGaaa                           <  2:758855/81‑1 (MQ=255)                                                         aTGGACAAGCTCCTGTTGCGCGACTTTCGCTTTGCGGCGTAACCACAATCCTTTCAGGACGATAATGAATGAAATGTTCa                     <  1:758858/80‑1 (MQ=255)                                                          tGGACAAGCTCCTGTTGCGCGACTTTCGCTTTGCGGCGTAACCACAATCCTTTCAGGACGATAATGAATGAAATGTTCAgc                   <  2:758858/81‑1 (MQ=255)                                                            gACAAGCTCCTGTTGCGCGACTTTCGCTTTGCGGCGTAACCACAATCCTTTCAGGACGATAATGAATGAAATGTTCAgcgc                 >  2:758902/1‑81 (MQ=255)                                                               aaGCTCCTGTTGCGCGACTTTCGCTTTGCGGCGTAACCACAATCCTTTCAGGACGATAATGAATGAAATGTTCAGCGCGAg              >  2:758933/1‑81 (MQ=255)                                                                 gCTCCTGTTGCGCGACTTTCGCTTTGCGGCGTAACCACAATCCTTTCAGGACGATAATGAATGAAATGTTCAGCGCGAGGc            <  1:758860/81‑1 (MQ=255)                                                                        ttGCGCGACTTTCGCTTTGCGGCGTAACCGCAATCCTTTCAGGACGATAATGAATGAAATGTTCAGCGCGAGGCCAATc       <  2:758862/79‑1 (MQ=255)                                                                        ttGCGCGACTTTCGCTTTGCGGCGTAACCACAATCCTTTCAGGACGATAATGAATGAAATGTTCAGCGCGAGGCCAATc       <  2:758860/79‑1 (MQ=255)                                                                        ttGCGCGACTTTCGCTTTGCGGCGTAACCACAATCCTTTCAGGACGATAATGAATGAAATGTTCAGCGCGAGGCCAATCg      <  2:758861/80‑1 (MQ=255)                                                                         tGCGCGACTTTCGCTTTGCGGCGTAACCACAATCCTTTCAGGACGATAATGAATGAAATGTTCAGCGCGAGGCCAATCGc     >  1:758905/1‑80 (MQ=255)                                                                          gcgcGACTTTCGCTTTGCGGCGTAACCACAATCCTTTCAGGACGATAATGAATGAAATGTTCAGCGCGAGGCCAATCGcc    <  2:758863/80‑1 (MQ=255)                                                                           cgcgACTTTCGCTTTGCGGCGTAACCACAATCCTTTCAGGACGATAATGAATGAAATGTTCAGCGCGAGGCCAATCGCCa   <  1:758865/80‑1 (MQ=255)                                                                           cgcgACTTTCGCTTTGCGGCGTAACCACAATCCTTTCAGGACGATAATGAATGAAATGTTCAGCGCGAGGCCAATCGCCAg  >  2:758924/1‑81 (MQ=255)                                                                            gcgACTTTCGCTTTGCGGCGTAACCACAATCCTTTCAGGACGATAATGAATGAAATGTTCAGCGCGAGGCCAATCGCCAg  >  1:758918/1‑80 (MQ=255)                                                                               |                                                                             GTTCATCGCCGCTGACGGCGCTGGCTTCGCACAACTGTTGCAGTAACTCAATGTTCATGGACAAGCTCCTGTTGCGCGGCTTTCGCTTTGCGGCGTAACCACAATCCTTTCAGGACGATAATGAATGAAATGTTCAGCGCGAGGCCAATCGCCAG  >  NC_000913/4090868‑4091022

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑