Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,093,381	G→A	R20C (CGT→TGT)	rhaM ←	L‑rhamnose mutarotase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,093,381	0	G	A	100.0%	175.8 / NA	48	R20C (CGT→TGT)	rhaM	L‑rhamnose mutarotase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (23/25);  total (23/25)

GAGATAGATGGCGTAGTTATGCGCACCGTGAGATTTCAGCACTGCTTCCAGTTCTGGCCAGATGGGATTATGCCGACGCTGATACTCTTCGTGGGCGTCGGGGTTTACCTGCATGACAAAGGCTTTGCGGATCATGTTTGCTCCTTACAGCGCC  >  NC_000913/4093304‑4093457                                                                              |                                                                             gagaTAGATGGCGTAGTTATGCGCACCGTGAGATTTCAGCACTGCTTCCAGTTCTGGCCAGATGGGATTATGCCGACACt                                                                            >  2:759508/1‑80 (MQ=255) gagaTAGATGGCGTAGTTATGCGCACCGTGAGATTTCAGCACTGCTTCCAGTTCTGGCCAGATGGGATTATGCCGACACt                                                                            <  1:759444/80‑1 (MQ=255)   gatagatGGCGTAGTTATGCGCACCGTGAGATTTCAGCACTGCTTCCAGTTCTGGCCAGATGGGATTATGCCGACACTGa                                                                          >  2:759506/1‑80 (MQ=255)       gatGGCGTAGTTATGCGCACCGTGAGATTTCAGCACTGCTTCCAGTTCTGGCCAGATGGGATTATGCCGACACTGATActc                                                                     <  2:759444/81‑1 (MQ=255)        atGGCGTAGTTATGCGCACCGTGAGATTTCAGCACTGCTTCCAGTTCTGGCCAGATGGGATTATGCCGACACTGATActct                                                                    >  2:759530/1‑81 (MQ=255)         tGGCGTAGTTATGCGCACCGTGAGATTTCAGCACTGCTTCCAGTTCTGGCCAGATGGGATTATGCCGACACTGATACTCtt                                                                   <  1:759446/81‑1 (MQ=255)         tGGCGTAGTTATGCGCACCGTGAGATTTCAGCACTGCTTCCAGTTCTGGCCAGATGGGATTATGCCGACACTGATACTCtt                                                                   >  2:759505/1‑81 (MQ=255)           gCGTAGTTATGCGCACCGTGAGATTTCAGCACTGCTTCCAGTTCTGGCCAGATGGGATTATGCCGACACTGATACTCTTc                                                                  <  1:759447/80‑1 (MQ=255)           gCGTAGTTATGCGCACCGTGAGATTTCAGCACTGCTTCCAGTTCTGGCCAGATGGGATTATGCCGACACTGATACTCTTc                                                                  <  1:759449/80‑1 (MQ=255)           gCGTAGTTATGCGCACCGTGAGATTTCAGCACTGCTTCCAGTTCTGGCCAGATGGGATTATGCCGACACTGATACTCTTc                                                                  <  2:759447/80‑1 (MQ=255)           gCGTAGTTATGCGCACCGTGAGATTTCAGCACTGCTTCCAGTTCTGGCCAGATGGGATTATGCCGACACTGATACTCTTCg                                                                 >  2:759514/1‑81 (MQ=255)             gTAGTTATGCGCACCGTGAGATTTCAGCACTGCTTCCAGTTCTGGCCAGATGGGATTATGCCGACACTGATACTCTTCGTg                                                               >  2:759525/1‑81 (MQ=255)                gTTATGCGCACCGTGAGATTTCAGCACTGCTTCCAGTTCTGGCCAGATGGGATTATGCCGACACTGATACTCTTCGTggg                                                             <  2:759450/80‑1 (MQ=255)                gTTATGCGCACCGTGAGATTTCAGCACTGCTTCCAGTTCTGGCCAGATGGGATTATGCCGACACTGATACTCTTCGTGGGc                                                            <  2:759449/81‑1 (MQ=255)                 ttATGCGCACCGTGAGATTTCAGCACTGCTTCCAGTTCTGGCCAGATGGGATTATGCCGACACTGATACTCTTCGTGGGCg                                                           >  1:759503/1‑81 (MQ=255)                    tGCGCACCGTGAGATTTCAGCACTGCTTCCAGTTCTGGCCAGATGGGATTATGCCGACACTGATACTCTTCGTGGGCGTCg                                                        >  2:759511/1‑81 (MQ=255)                       gcACCGTGAGATTTCAGCACTGCTTCCAGTTCTGGCCAGATGGGATTATGCCGACACTGATACTCTTCGTGGGCGTCgggg                                                     <  2:759451/81‑1 (MQ=255)                       gcACCGTGAGATTTCAGCACTGCTTCCAGTTCTGGCCAGATGGGATTATGCCGACACTGATACTCTTCGTGGGCGTCgggg                                                     <  1:759453/81‑1 (MQ=255)                         aCCGTGAGATTTCAGCACTGCTTCCAGTTCTGGCCAGATGGGATTATGCCGACACTGATACTCTTCGTGGGCGTCGGGGtt                                                   >  1:759517/1‑81 (MQ=255)                           cGTGAGATTTCAGCACTGCTTCCAGTTCTGGCCAGATGGGATTATGCCGACACTGATACTCTTCGTGGGCGTCGGGGTTTa                                                 <  2:759453/81‑1 (MQ=255)                             tGAGATTTCAGCACTGCTTCCAGTTCTGGCCAGATGGGATTATGCCGACACTGATACTCTTCGTGGGCGTCGGGGTTTAcc                                               <  1:759455/81‑1 (MQ=255)                              gagaTTTCAGCACTGCTTCCAGTTCTGGCCAGATGGGATTATGCCGACACTGATACTCTTCGTGGGCGTCGGGGTTTACCt                                              >  1:759523/1‑81 (MQ=255)                               agaTTTCAGCACTGCTTCCAGTTCTGGCCAGATGGGATTATGCCGACACTGATACTCTTCGTGGGCGTCGGGGTTTACCTg                                             >  2:759518/1‑81 (MQ=255)                               agaTTTCAGCACTGCTTCCAGTTCTGGCCAGATGGGATTATGCCGACACTGATACTCTTCGTGGGCGTCGGGGTTTACCTg                                             >  1:759511/1‑81 (MQ=255)                               agaTTTCAGCACTGCTTCCAGTTCTGGCCAGATGGGATTATGCCGACACTGATACTCTTCGTGGGCGTCGGGGTTTACCTg                                             >  1:759505/1‑81 (MQ=255)                                gaTTTCAGCACTGCTTCCAGTTCTGGCCAGATGGGATTATGCCGACACTGATACTCTTCGTGGGCGTCGGGGTTTACCTg                                             >  1:759536/1‑80 (MQ=255)                                 aTTTCAGCACTGCTTCCAGTTCTGGCCAGATGGGATTATGCCGACACTGATACTCTTCGTGGGCGTCGGGGTTTACCTGCa                                           >  2:759521/1‑81 (MQ=255)                                  tttCAGCACTGCTTCCAGTTCTGGCCAGATGGGATTATGCCGACACTGATACTCTTCGTGGGCGTCGGGGTTTACCTGCAt                                          <  2:759455/81‑1 (MQ=255)                                    tCAGCACTGCTTCCAGTTCTGGCCAGATGGGATTATGCCGACACTGATACTCTTCGTGGGCGTCGGGGTTTACCTGCATg                                         <  2:759456/80‑1 (MQ=255)                                    tCAGCACTGCTTCCAGTTCTGGCCAGATGGGATTATGCCGACACTGATACTCTTCGTGGGCGTCGGGGTTTACCTGCATGa                                        >  1:759533/1‑81 (MQ=255)                                        cACTGCTTCCAGTTCTGGCCAGATGGGATTATGCCGACACTGATACTCTTCGTGGGCGTCGGGGTTTACCTGCATGACaaa                                    <  1:759458/81‑1 (MQ=255)                                             cTTCCAGTTCTGGCCAGATGGGATTATGCCGACACTGATACTCTTCGTGGGCGTCGGGGTTTACCTGCATGACAAAGGCtt                               >  2:759512/1‑81 (MQ=255)                                              ttCCAGTTCTGGCCAGATGGGATTATGCCGACACTGATACTCTTCGTGGGCGTCGGGGTTTACCTGCATGACAAAGGCttt                              >  2:759515/1‑81 (MQ=255)                                                  aGTTCTGGCCAGATGGGATTATGCCGACACTGATACTCTTCGTGGGCGTCGGGGTTTACCTGCATGACAAAGGCTTTGCgg                          <  2:759458/81‑1 (MQ=255)                                                  aGTTCTGGCCAGATGGGATTATGCCGACACTGATACTCTTCGTGGGCGTCGGGGTTTACCTGCATGACAAAGGCTTTGCg                           >  2:759523/1‑80 (MQ=255)                                                   gTTCTGGCCAGATGGGATTATGCCGACACTGATACTCTTCGTGGGCGTCGGGGTTTACCTGCATGACAAAGGCTTTGCGGa                         >  2:759509/1‑81 (MQ=255)                                                       tGGCCAGATGGGATTATGCCGACACTGATACTCTTCGTGGGCGTCGGGGTTTACCTGCATGACAAAGGCTTTGCGGATCAt                     >  2:759531/1‑81 (MQ=255)                                                         gCCAGATGGGATTATGCCGACACTGATACTCTTCGTGGGCGTCGGGGTTTACCTGCATGACAAAGGCTTTGCGGATCAt                     <  2:759459/79‑1 (MQ=255)                                                          ccAGATGGGATTATGCCGACACTGATACTCTTCGTGGGCGTCGGGGTTTACCTGCATGACAAAGGCTTTGCGGATCATGtt                  <  2:759460/81‑1 (MQ=255)                                                           cAGATGGGATTATGCCGACACTGATACTCTTCGTGGGCGTCGGGGTTTACCTGCATGACAAAGGCTTTGCGGATCATGttt                 <  2:759461/81‑1 (MQ=255)                                                            aGATGGGATTATGCCGACACTGATACTCTTCGTGGGCGTCGGGGTTTACCTGCATGACAAAGGCTTTGCGGATCATGTTTg                <  2:759462/81‑1 (MQ=255)                                                               tGGGATTATGCCGACACTGATACTCTTCGTGGGCGTCGGGGTTTACCTGCATGACAAAGGCTTTGCGGATCATGTTTGCTc             <  2:759463/81‑1 (MQ=255)                                                                 ggATTATGCCGACACTGATACTCTTCGTGGGCGTCGGGGTTTACCTGCATGACAAAGGCTTTGCGGATCATGTTTGCTcc            <  1:759465/80‑1 (MQ=255)                                                                 ggATTATGCCGACACTGATACTCTTCGTGGGCGTCGGGGTTTACCTGCATGACAAAGGCTTTGCGGATCATGTTTGCTCCt           >  1:759527/1‑81 (MQ=255)                                                                  gATTATGCCGACACTGATACTCTTCGTGGGCGTCGGGGTTTACCTGCATGACAAAGGCTTTGCGGATCATGTTTGCTCCtt          <  2:759465/81‑1 (MQ=255)                                                                    ttATGCCGACACTGATACTCTTCGTGGGCGTCGGGGTTTACCTGCATGACAAAGGCTTTGCGGATCATGTTTGCTCCTTAc        <  2:759466/81‑1 (MQ=255)                                                                     tATGCCGACACTGATACTCTTCGTGGGCGTCGGGGTTTACCTGCATGACAAAGGCTTTGCGGATCATGTTTGCTCCTTa         >  2:759528/1‑79 (MQ=255)                                                                          cGACACTGATACTCTTCGTGGGCGTCGGGGTTTACCTGCATGACAAAGGCTTTGCGGATCATGTTTGCTCCTTAcagcgcc  <  2:759467/81‑1 (MQ=255)                                                                              |                                                                             GAGATAGATGGCGTAGTTATGCGCACCGTGAGATTTCAGCACTGCTTCCAGTTCTGGCCAGATGGGATTATGCCGACGCTGATACTCTTCGTGGGCGTCGGGGTTTACCTGCATGACAAAGGCTTTGCGGATCATGTTTGCTCCTTACAGCGCC  >  NC_000913/4093304‑4093457

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑