Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,108,387	G→C	G279A (GGT→GCT)	pfkA →	6‑phosphofructokinase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,108,387	0	G	C	100.0%	148.2 / NA	42	G279A (GGT→GCT)	pfkA	6‑phosphofructokinase I
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (24/18);  total (24/18)

TTCTCCGGTGCCTTACGACCGTATTCTGGCTTCCCGTATGGGCGCTTACGCTATCGATCTGCTGCTGGCAGGTTACGGCGGTCGTTGTGTAGGTATCCAGAACGAACAGCTGGTTCACCACGACATCATCGACGCTATCGAAAACATGA  >  NC_000913/4108316‑4108464                                                                        |                                                                              ttCTCCGGTGCCTTACGACCGTATTCTGGCTTCCCGTATGGGCGCTTACGCTATCGATCTGCTGCTGGCAGCTTAcggcgg                                                                      >  1:763653/1‑81 (MQ=255) ttCTCCGGTGCCTTACGACCGTATTCTGGCTTCCCGTATGGGCGCTTACGCTATCGATCTGCTGCTGGCAGCTTAcggcgg                                                                      >  2:763627/1‑81 (MQ=255)   ctcCGGTGCCTTACGACCGTATTCTGGCTTCCCGTATGGGCGCTTACGCTATCGATCTGCTGCTGGCAGCTTACGGCGGTc                                                                    >  2:763636/1‑81 (MQ=255)     ccGGTGCCTTACGACCGTATTCTGGCTTCCCGTATGGGCGCTTACGCTATCGATCTGCTGCTGGCAGCTTACGGCGGTCGt                                                                  >  2:763623/1‑81 (MQ=255)      cGGTGCCTTACGACCGTATTCTGGCTTCCCGTATGGGCGCTTACGCTATCGATCTGCTGCTGGCAGCTTACGGCGGTCGtt                                                                 <  1:763566/81‑1 (MQ=255)       ggTGCCTTACGACCGTATTCTGGCTTCCCGTATGGGCGCTTACGCTATCGATCTGCTGCTGGCAGCTTACGGCGGTCGTtg                                                                <  1:763567/81‑1 (MQ=255)       ggTGCCTTACGACCGTATTCTGGCTTCCCGTATGGGCGCTTACGCTATCGATCTGCTGCTGGCAGCTTACGGCGGTCGTtg                                                                >  2:763647/1‑81 (MQ=255)        gTGCCTTACGACCGTATTCTGGCTTCCCGTATGGGCGCTTACGCTATCGATCTGCTGCTGGCAGCTTACGGCGGTCGTtgt                                                               >  2:763632/1‑81 (MQ=255)        gTGCCTTACGACCGTATTCTGGCTTCCCGTATGGGCGCTTACGCTATCGATCTGCTGCTGGCAGCTTACGGCGGTCGTtgt                                                               <  1:763568/81‑1 (MQ=255)        gTGCCTTACGACCGTATTCTGGCTTCCCGTATGGGCGCTTACGCTATCGATCTGCTGCTGGCAGCTTACGGCGGTCGTtgt                                                               >  2:763617/1‑81 (MQ=255)           ccTTACGACCGTATTCTGGCTTCCCGTATGGGCGCTTACGCTATCGATCTGCTGCTGGCAGCTTACGGCGGTCGTtgtg                                                              >  1:763639/1‑79 (MQ=255)               aCGACCGTATTCTGGCTTCCCGTATGGGCGCTTACGCTATCGATCTGCTGCTGGCAGCTTACGGCGGTCGTTGTGTAGGt                                                         <  2:763568/80‑1 (MQ=255)                cGACCGTATTCTGGCTTCCCGTATGGGCGCTTACGCTATCGATCTGCTGCTGGCAGCTTACGGCGGTCGTTGTGTAGGTAt                                                       >  1:763622/1‑81 (MQ=255)                    cGTATTCTGGCTTCCCGTATGGGCGCTTACGCTATCGATCTGCTGCTGGCAGCTTACGGCGGTCGTTGTGTAGGTATCCAg                                                   <  1:763570/81‑1 (MQ=255)                     gTATTCTGGCTTCCCGTATGGGCGCTTACGCTATCGATCTGCTGCTGGCAGCTTACGGCGGTCGTTGTGTAGGTATCCAga                                                  <  2:763570/81‑1 (MQ=255)                     gTATTCTGGCTTCCCGTATGGGCGCTTACGCTATCGATCTGCTGCTGGCAGCTTACGGCGGTCGTTGTGTAGGTATCCAg                                                   >  1:763627/1‑80 (MQ=255)                     gTATTCTGGCTTCCCGTATGGGCGCTTACGCTATCGATCTGCTGCTGGCAGCTTACGGCGGTCGTTGTGTAGGTATCCAg                                                   >  1:763646/1‑80 (MQ=255)                            ggCTTCCCGTATGGGCGCTTACGCTATCGATCTGCTGCTGGCAGCTTACGGCGGTCGTTGTGTAGGTATCCAgaacgaa                                             >  1:763634/1‑79 (MQ=255)                             gCTTCCCGTATGGGCGCTTACGCTATCGATCTGCTGCTGGCAGCTTACGGCGGTCGTTGTGTAGGTATCCAGAACGAACAg                                          <  2:763571/81‑1 (MQ=255)                              cTTCCCGTATGGGCGCTTACGCTATCGATCTGCTGCTGGCAGCTTACGGCGGTCGTTGTGTAGGTATCCAGAACGAACAGc                                         <  2:763572/81‑1 (MQ=255)                                 cccGTATGGGCGCTTACGCTATCGATCTGCTGCTGGCAGCTTACGGCGGTCGTTGTGTAGGTATCCAGAACGAACAGCt                                        <  1:763574/79‑1 (MQ=255)                                  ccGTATGGGCGCTTACGCTATCGATCTGCTGCTGGCAGCTTACGGCGGTCGTTGTGTAGGTATCCAGAACGAACAGCTGGt                                     >  2:763624/1‑81 (MQ=255)                                   cGTATGGGCGCTTACGCTATCGATCTGCTGCTGGCAGCTTACGGCGGTCGTTGTGTAGGTATCCAGAACGAACAGCTGGtt                                    <  2:763574/81‑1 (MQ=255)                                    gTATGGGCGCTTACGCTATCGATCTGCTGCTGGCAGCTTACGGCGGTCGTTGTGTAGGTATCCAGAACGAACAGCTGGtt                                    >  1:763647/1‑80 (MQ=255)                                         ggCGCTTACGCTATCGATCTGCTGCTGGCAGCTTACGGCGGTCGTTGTGTAGGTATCCAGAACGAACAGCTGGTTcaccac                              <  2:763575/81‑1 (MQ=255)                                            gcTTACGCTATCGATCTGCTGCTGGCAGCTTACGGCAGTCGTTGTGTAGGTATCCAGAACGAACAGCTGGTTCACCACGAc                           <  1:763577/81‑1 (MQ=255)                                             cTTACGCTATCGATCTGCTGCTGGCAGCTTACGGCGGTCGTTGTGTAGGTATCCAGAACGAACAGCTGGTTCACCACGAca                          >  1:763652/1‑81 (MQ=255)                                             cTTACGCTATCGATCTGCTGCTGGCAGCTTACGGCGGTCGTTGTGTAGGTATCCAGAACGAACAGCTGGTTCACCACGAca                          <  1:763578/81‑1 (MQ=255)                                              ttACGCTATCGATCTGCTGCTGGCAGCTTACGGCGGTCGTTGTGTAGGTATCCAGAACGAACAGCTGGTTCACCACGAcat                         >  2:763635/1‑81 (MQ=255)                                                aCGCTATCGATCTGCTGCTGGCAGCTTACGGCGGTCGTTGTGTAGGTATCCAGAACGAACAGCTGGTTCACCACGAcat                         <  1:763579/79‑1 (MQ=255)                                                 cGCTATCGATCTGCTGCTGGCAGCTTACGGCGGTCGTTGTGTAGGTATCCAGAACGAACAGCTGGTTCACCACGAcatcat                      <  1:763580/81‑1 (MQ=255)                                                  gCTATCGATCTGCTGCTGGCAGCTTACGGCGGTCGTTGTGTAGGTATCCAGAACGAACAGCTGGTTCACCACGACatcatc                     >  1:763632/1‑81 (MQ=255)                                                    tATCGATCTGCTGCTGGCAGCTTACGGCGGTCGTTGTGTAGGTATCCAGAACGAACAGCTGGTTCACCACGACATCATCGa                   >  2:763637/1‑81 (MQ=255)                                                     aTCGATCTGCTGCTGGCAGCTTACGGCGGTCGTTGTGTAGGTATCCAGAACGAACAGCTGGTTCACCACGACATCATCGAc                  >  2:763625/1‑81 (MQ=255)                                                     aTCGATCTGCTGCTGGCAGCTTACGGCGGTCGTTGTGTAGGTATCCAGAACGAACAGCTGGTTCACCACGACATCATCGAc                  >  2:763660/1‑81 (MQ=255)                                                             gctgctgGCAGCTTACGGCGGTCGTTGTGTAGGTATCCAGAACGAACAGCTGGTTCACCACGACATCATCGACGCTATCg           >  1:763635/1‑80 (MQ=255)                                                              ctgctgGCAGCTTACGGCGGTCGTTGTGTAGGTATCCAGAACGAACAGCTGGTTCACCACGACATCATCGACGCTATCGaa         <  2:763580/81‑1 (MQ=255)                                                              ctgctgGCAGCTTACGGCGGTCGTTGTGTAGGTATCCAGAACGAACAGCTGGTTCACCACGACATCATCGACGCTATCGa          >  1:763642/1‑80 (MQ=255)                                                                gctgGCAGCTTACGGCGGTCGTTGTGTAGGTATCCAGAACGAACAGCTGGTTCACCACGACATCATCGACGCTATCGaaaa       >  1:763651/1‑81 (MQ=255)                                                                gctgGCAGCTTACGGCGGTCGTTGTGTAGGTATCCAGAACGAACAGCTGGTTCACCACGACATCATCGACGCTATCGaaaa       >  1:763644/1‑81 (MQ=255)                                                                    gCAGCTTACGGCGGTCGTTGTGTAGGTATCCAGAACGAACAGCTGGTTCACCACGACATCATCGACGCTATCGAAAACa     <  2:763581/79‑1 (MQ=255)                                                                     cAGCTTACGGCGGTCGTTGTGTAGGTATCCAGAACGAACAGCTGGTTCACCACGACATCATCGACGCTATCGAAAACATGa  <  1:763583/81‑1 (MQ=255)                                                                        |                                                                              TTCTCCGGTGCCTTACGACCGTATTCTGGCTTCCCGTATGGGCGCTTACGCTATCGATCTGCTGCTGGCAGGTTACGGCGGTCGTTGTGTAGGTATCCAGAACGAACAGCTGGTTCACCACGACATCATCGACGCTATCGAAAACATGA  >  NC_000913/4108316‑4108464

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑