Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,142,492	A→T	intergenic (‑271/‑38)	ptsA ← / → frwC	putative PTS multiphosphoryl transfer protein PtsA/putative PTS enzyme IIC component FrwC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,142,492	0	A	T	100.0%	111.9 / NA	31	intergenic (‑271/‑38)	ptsA/frwC	putative PTS multiphosphoryl transfer protein PtsA/putative PTS enzyme IIC component FrwC
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (13/18);  total (13/18)

AAAGTCCAGTAAATGGCCTTTTTATCCACTGTTTGCTCCGCCTGCGATTGCCTATTGTTTCCTCCATCAAAAATATGGACATGGGCTTTCGCAAGCCCGGGAGCAGCTTATGAATGAGTTGGTGCAGATCCTGAAAAATACCCGTCAGC  >  NC_000913/4142421‑4142569                                                                        |                                                                              aaaGTCCAGTAAATGGCCTTTTTATCCACTGTTTGCTCCGCCTGCGATTGCCTATTGTTTCCTCCATCAAATATATGGACa                                                                      >  1:772979/1‑81 (MQ=255)  aaGTCCAGTAAATGGCCTTTTTATCCACTGTTTGCTCCGCCTGCGATTGCCTATTGTTTCCTCCATCAAATATATGGACAt                                                                     <  1:772944/81‑1 (MQ=255)  aaGTCCAGTAAATGGCCTTTTTATCCACTGTTTGCTCCGCCTGCGATTGCCTATTGTTTCCTCCATCAAATATATGGACAt                                                                     <  2:772944/81‑1 (MQ=255)        aGTAAATGGCCTTTTTATCCACTGTTTGCTCCGCCTGCGATTGCCTATTGTTTCCTCCATCAAATATATGGACATGGGCtt                                                               >  1:772972/1‑81 (MQ=255)          tAAATGGCCTTTTTATCCACTGTTTGCTCCGCCTGCGATTGCCTATTGTTTCCTCCATCAAATATATGGACATGGGCTTTc                                                             <  2:772945/81‑1 (MQ=255)              tGGCCTTTTTATCCACTGTTTGCTCCGCCTGCGATTGCCTATTGTTTCCTCCATCAAATATATGGACATGGGCTTTCGCaa                                                         <  1:772947/81‑1 (MQ=255)                 ccTTTTTATCCACTGTTTGCTCCGCCTGCGATTGCCTATTGTTTCCTCCATCAAATATATGGACATGGGCTTTCGCAAGcc                                                      >  2:772989/1‑81 (MQ=255)                  cTTTTTATCCACTGTTTGCTCCGCCTGCGATTGCCTATTGTTTCCTCCATCAAATATATGGACATGGGCTTTCGCAAGccc                                                     <  2:772947/81‑1 (MQ=255)                      ttATCCACTGTTTGCTCCGCCTGCGATTGCCTATTGTTTCCTCCATCAAATATATGGACATGGGCTTTCGCAAGCCCggg                                                  <  2:772948/80‑1 (MQ=255)                       tATCCACTGTTTGCTCCGCCTGCGATTGCCTATTGTTTCCTCCATCAAATATATGGACATGGGCTTTCGCAAGCCCGGGa                                                 <  1:772950/80‑1 (MQ=255)                          ccACTGTTTGCTCCGCCTGCGATTGCCTATTGTTTCCTCCATCAAATATATGGACATGGGCTTTCGCAAGCCCGGGagcag                                             >  2:772988/1‑81 (MQ=255)                           cACTGTTTGCTCCGCCTGCGATTGCCTATTGTTTCCTCCATCAAATATATGGACATGGGCTTTCGCAAGCCCGGGagcagc                                            >  1:772982/1‑81 (MQ=255)                                   gCTCCGCCTGCGATTGCCTATTGTTTCCTCCATCAAATATATGGACATGGGCTTTCGCAAGCCCGGGAGCAGCTTatgaat                                    >  2:772985/1‑81 (MQ=255)                                    cTCCGCCTGCGATTGCCTATTGTTTCCTCCATCAAATATATGGACATGGGCTTTCGCAAGCCCGGGAGCAGCTTatgaatg                                   <  2:772952/81‑1 (MQ=255)                                    cTCCGCCTGCGATTGCCTATTGTTTCCTCCATCAAATATATGGACATGGGCTTTCGCAAGCCCGGGAGCAGCTTatgaat                                    <  1:772952/80‑1 (MQ=255)                                    cTCCGCCTGCGATTGCCTATTGTTTCCTCCATCAAATATATGGACATGGGCTTTCGCAAGCCCGGGAGCAGCTTatgaat                                    <  1:772951/80‑1 (MQ=255)                                       cGCCTGCGATTGCCTATTGTTTCCTCCATCAAATATATGGACATGGGCTTTCGCAAGCCCGGGAGCAGCTTATGAATGAGt                                >  1:772993/1‑81 (MQ=255)                                        gCCTGCGATTGCCTATTGTTTCCTCCATCAAATATATGGACATGGGCTTTCGCAAGCCCGGGAGCAGCTTATGAATGAGtt                               <  1:772954/81‑1 (MQ=255)                                         ccTGCGATTGCCTATTGTTTCCTCCATCAAATATATGGACATGGGCTTTCGCAAGCCCGGGAGCAGCTTATGAATGAGTTg                              >  1:772994/1‑81 (MQ=255)                                          cTGCGATTGCCTATTGTTTCCTCCATCAAATATATGGACATGGGCTTTCGCAAGCCCGGGAGCAGCTTATGAATGAGTTgg                             >  1:772996/1‑81 (MQ=255)                                            gCGATTGCCTATTGTTTCCTCCATCAAATATATGGACATGGGCTTTCGCAAGCCCGGGAGCAGCTTATGAATGAGTTGGTg                           >  1:772987/1‑81 (MQ=255)                                             cGATTGCCTATTGTTTCCTCCATCAAATATATGGACATGGGCTTTCGCAAGCCCGGGAGCAGCTTATGAATGAGTTGGTg                           >  2:773003/1‑80 (MQ=255)                                                   ccTATTGTTTCCTCCATCAAATATATGGACATGGGCTTTCGCAAGCCCGGGAGCAGCTTATGAATGAGTTGGTGCAGATcc                    >  1:772991/1‑81 (MQ=255)                                                   ccTATTGTTTCCTCCATCAAATATATGGACATGGGCTTTCGCAAGCCCGGGAGCAGCTTATGAATGAGTTGGTGCAGATcc                    <  2:772954/81‑1 (MQ=255)                                                           ttCCTCCATCAAATATATGGACATGGGCTTTCGCAAGCCCGGGAGCAGCTTATGAATGAGTTGGTGCAGATCCTGaaaaa             >  2:772987/1‑80 (MQ=255)                                                              ctccATCAAATATATGGACATGGGCTTTCGCAAGCCCGGGAGCAGCTTATGAATGAGTTGGTGCAGATCCTGAAAAATAcc         <  2:772956/81‑1 (MQ=255)                                                              ctccATCAAATATATGGACATGGGCTTTCGCAAGCCCGGGAGCAGCTTATGAATGAGTTGGTGCAGATCCTGAAAAATAcc         <  1:772956/81‑1 (MQ=255)                                                              ctccATCAAATATATGGACATGGGCTTTCGCAAGCCCGGGAGCAGCTTATGAATGAGTTGGTGCAGATCCTGAAAAATAc          <  1:772958/80‑1 (MQ=255)                                                                 cATCAAATATATGGACATGGGCTTTCGCAAGCCCGGGAGCAGCTTATGAATGAGTTGGTGCAGATCCTGAAAAATACCCg       <  1:772959/80‑1 (MQ=255)                                                                 cATCAAATATATGGACATGGGCTTTCGCAAGCCCGGGAGCAGCTTATGAATGAGTTGGTGCAGATCCTGAAAAATACCCGt      <  2:772959/81‑1 (MQ=255)                                                                   tCAAATATATGGACATGGGCTTTCGCAAGCCCGGGAGCAGCTTGTGAATGAGTTGGTGCAGATCCTGAAAAATACCCGTCa    <  1:772961/81‑1 (MQ=255)                                                                     aaataTATGGACATGGGCTTTCGCAAGCCCGGGAGCAGCTTATGAATGAGTTGGTGCAGATCCTGAAAAATACCCGTCAg   >  2:773001/1‑80 (MQ=255)                                                                     aaataTATGGACATGGGCTTTCGCAAGCCCGGGAGCAGCTTATGAATGAGTTGGTGCAGATCCTGAAAAATACCCGTCAGc  <  1:772962/81‑1 (MQ=255)                                                                        |                                                                              AAAGTCCAGTAAATGGCCTTTTTATCCACTGTTTGCTCCGCCTGCGATTGCCTATTGTTTCCTCCATCAAAAATATGGACATGGGCTTTCGCAAGCCCGGGAGCAGCTTATGAATGAGTTGGTGCAGATCCTGAAAAATACCCGTCAGC  >  NC_000913/4142421‑4142569

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑