Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	396,964	A→G	N109S (AAC→AGC)	sbmA →	peptide antibiotic/peptide nucleic acid transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	396,964	0	A	G	100.0%	117.9 / NA	34	N109S (AAC→AGC)	sbmA	peptide antibiotic/peptide nucleic acid transporter
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (21/13);  total (21/13)

GCAATACTGGTCAATACTCGGTACTGCACTGATCATCTTCGTCACCTGGTTTTTGGTGGAAGTCGGGGTCGCCGTCAACGCCTGGTATGCGCCGTTCTATGATCTGATTCAAACCGCGCTAAGTTCGCCGCATAAAGTCACCATCGAACAATTT  >  NC_000913/396887‑397040                                                                              |                                                                             gCAATACTGGTCAATACTCGGTACTGCACTGATCATCTTCGTCACCTGGTTTTTGGTGGAAGTCGGGGTCGCCGTCAgcgc                                                                           <  1:81191/81‑1 (MQ=255)   aaTACTGGTCAATACTCGGTACTGCACTGATCATCTTCGTCACCTGGTTTTTGGTGGAAGTCGGGGTCGCCGTCAGCGCCt                                                                         >  2:81215/1‑81 (MQ=255)   aaTACTGGTCAATACTCGGTACTGCACTGATCATCTTCGTCACCTGGTTTTTGGTGGAAGTCGGGGTCGCCGTCAGCGCCt                                                                         <  2:81192/81‑1 (MQ=255)    aTACTGGTCAATACTCGGTACTGCACTGATCATCTTCGTCACCTGGTTTTTGGTGGAAGTCGGGGTCGCCGTCAGCGCCTg                                                                        <  2:81193/81‑1 (MQ=255)         ggTCAATACTCGGTACTGCACTGATCATCTTCGTCACCTGGTTTTTGGTGGAAGTCGGGGTCGCCGTCAGCGCCTGGTATg                                                                   >  2:81216/1‑81 (MQ=255)          gTCAATACTCGGTACTGCACTGATCATCTTCGTCACCTGGTTTTTGGTGGAAGTCGGGGTCGCCGTCAGCGCCTGGTAt                                                                    <  2:81194/79‑1 (MQ=255)             aaTACTCGGTACTGCACTGATCATCTTCGTCACCTGGTTTTTGGTGGAAGTCGGGGTCGCCGTCAGCGCCTGGTATgcgc                                                                <  2:81195/80‑1 (MQ=255)             aaTACTCGGTACTGCACTGATCATCTTCGTCACCTGGTTTTTGGTGGAAGTCGGGGTCGCCGTCAGCGCCTGGTATGCGcc                                                               >  2:81221/1‑81 (MQ=255)               tACTCGGTACTGCACTGATCATCTTCGTCACCTGGTTTTTGGTGGAAGTCGGGGTCGCCGTCAGCGCCTGGTATGCGCCGt                                                             >  2:81227/1‑81 (MQ=255)                    ggTACTGCACTGATCATCTTCGTCACCTGGTTTTTGGTGGAAGTCGGGGTCGCCGTCAGCGCCTGGTATGCGCCGTTCt                                                          <  2:81196/79‑1 (MQ=255)                          gCACTGATCATCTTCGTCACCTGGTTTTTGGTGGAAGTCGGGGTCGCCGTCAGCGCCTGGTATGCGCCGTTCTATGATCt                                                   >  2:81228/1‑80 (MQ=255)                           cACTGATCATCTTCGTCACCTGGTTTTTGGTGGAAGTCGGGGTCGCCGTCAGCGCCTGGTATGCGCCGTTCTATGATCt                                                   <  1:81197/79‑1 (MQ=255)                             cTGATCATCTTCGTCACCTGGTTTTTGGTGGAAGTCGGGGTCGCCGTCAGCGCCTGGTATGCGCCGTTCTATGATCTGAtt                                               <  2:81198/81‑1 (MQ=255)                                  catcTTCGTCACCTGGTTTTTGGTGGAAGTCGGGGTCGCCGTCAGCGCCTGGTATGCGCCGTTCTATGATCTGATTCAAAc                                          >  2:81223/1‑81 (MQ=255)                                  catcTTCGTCACCTGGTTTTTGGTGGAAGTCGGGGTCGCCGTCAGCGCCCGGTATGCGCCGTTCTATGATCTGATTCAAAc                                          >  2:81229/1‑81 (MQ=255)                                   atcTTCGTCACCTGGTTTTTGGTGGAAGTCGGGGTCGCCGTCAGCGCCTGGTATGCGCCGTTCTATGATCTGATTCAAAcc                                         >  2:81234/1‑81 (MQ=255)                                   atcTTCGTCACCTGGTTTTTGGTGGAAGTCGGGGTCGCCGTCAGCGCCTGGTATGCGCCGTTCTATGATCTGATTCAAAc                                          <  1:81199/80‑1 (MQ=255)                                           cACCTGGTTTTTGGTGGAAGTCGGGGTCGCCGTCAGCGCCTGGTATGCGCCGTTCTATGATCTGATTCAAACCGCGCTAAg                                 >  2:81222/1‑81 (MQ=255)                                            aCCTGGTTTTTGGTGGAAGTCGGGGTCGCCGTCAGCGCCTGGTATGCGCCGTTCTATGATCTGATTCAAACCGCGCTAAGt                                <  2:81200/81‑1 (MQ=255)                                            aCCTGGTTTTTGGTGGAAGTCGGGGTCGCCGTCAGCGCCTGGTATGCGCCGTTCTATGATCTGATTCAAACCGCGCTAAGt                                >  1:81236/1‑81 (MQ=255)                                              cTGGTTTTTGGTGGAAGTCGGAGTCGCCGTCAGCGCCTGGTATGCGCCGTTCTATGATCTGATTCAAACCGCGCTAAGTTc                              <  2:81201/81‑1 (MQ=255)                                                      tggtggAAGTCGGGGTCGCCGTCAGCGCCTGGTATGCGCCGTTCTATGATCTGATTCAAACCGCGCTAAGTTCGCCGCAt                       >  1:81242/1‑80 (MQ=255)                                                       ggtggAAGTCGGGGTCGCCGTCAGCGCCTGGTATGCGCCGTTCTATGATCTGATTCAAACCGCGCTAAGTTCGCCGCATa                      >  1:81226/1‑80 (MQ=255)                                                              gTCGGGGTCGCCGTCAGCGCCTGGTATGCGCCGTTCTATGATCTGATTCAAACCGCGCTAAGTTCGCCGCATAAAGTCAc               >  1:81230/1‑80 (MQ=255)                                                                 ggggTCGCCGTCAGCGCCTGGTATGCGCCGTTCTATGATCTGATTCAAACCGCGCTAAGTTCGCCGCATAAAGTCACCATc           <  1:81202/81‑1 (MQ=255)                                                                 ggggTCGCCGTCAGCGCCTGGTATGCGCCGTTCTATGATCTGATTCAAACCGCGCTAAGTTCGCCGCATAAAGTCACCATc           >  2:81237/1‑81 (MQ=255)                                                                 ggggTCGCCGTCAGCGCCTGGTATGCGCCGTTCTATGATCTGATTCAAACCGCGCTAAGTTCGCCGCATAAAGTCACCATc           >  2:81240/1‑81 (MQ=255)                                                                      cGCCGTCAGCGCCTGGTATGCGCCGTTCTATGATCTGATTCAAACCGCGCTAAGTTCGCCGCATAAAGTCACCATCGAAc       >  2:81241/1‑80 (MQ=255)                                                                       gCCGTCAGCGCCTGGTATGCGCCGTTCTATGATCTGATTCAAACCGCGCTAAGTTCGCCGCATAAAGTCACCATCGAACaa     >  1:81238/1‑81 (MQ=255)                                                                       gCCGTCAGCGCCTGGTATGCGCCGTTCTATGATCTGATTCAAACCGCGCTAAGTTCGCCGCATAAAGTCACCATCGAACaa     >  2:81243/1‑81 (MQ=255)                                                                       gCCGTCAGCGCCTGGTATGCGCCGTTCTATGATCTGATTCAAACCGCGCTAAGTTCGCCGCATAAAGTCACCATCGAACa      <  2:81203/80‑1 (MQ=255)                                                                       gCCGTCAGCGCCTGGTATGCGCCGTTCTATGATCTGATTCAAACCGCGCTAAGTTCGCCGCATAAAGTCACCATCGAACa      <  1:81204/80‑1 (MQ=255)                                                                          gTCAGCGCCTGGTATGCGCCGTTCTATGATCTGATTCAAACCGCGCTAAGTTCGCCGCATAAAGTCACCATCGAACAAttt  >  2:81244/1‑81 (MQ=255)                                                                           tCAGCGCCTGGTATGCGCCGTTCTATGATCTGATTCAAACCGCGCTAAGTTCGCCGCATAAAGTCACCATCGAACAAttt  >  1:81231/1‑80 (MQ=255)                                                                           tCAGCGCCTGGTATGCGCCGTTCTATGATCTGATTCAAACCGCGCTAAGTTCGCCGCATAAAGTCACCATCGAACAAttt  >  2:81248/1‑80 (MQ=255)                                                                              |                                                                             GCAATACTGGTCAATACTCGGTACTGCACTGATCATCTTCGTCACCTGGTTTTTGGTGGAAGTCGGGGTCGCCGTCAACGCCTGGTATGCGCCGTTCTATGATCTGATTCAAACCGCGCTAAGTTCGCCGCATAAAGTCACCATCGAACAATTT  >  NC_000913/396887‑397040

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑