Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,147,894	C→A	A142S (GCA→TCA)	yijO ←	putative DNA‑binding transcriptional regulator YijO

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,147,894	0	C	A	100.0%	156.1 / NA	43	A142S (GCA→TCA)	yijO	putative DNA‑binding transcriptional regulator YijO
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (20/23);  total (20/23)

GCTGCGTGAGGCGGTCTGGATTTGGCTGCCAAGCAAATCGCGGCAGTGGCTAAGCAGGCTGGCGACGATAAGCCTTGCCGTTTGCTGCTCCTGCGGCTGCATTTGCATTTCATTGAGCGTTTGTAGCAGAAAAGAACCTATGCGTGGGCCGCG  >  NC_000913/4147817‑4147969                                                                              |                                                                            gCTGCGTGAGGCGGTCTGGATTTGGCTGCCAAGCAAATCGCGGCAGTGGCTAAGCAGGCTGGCGACGATAAGCCTTGACg                                                                           <  2:774398/80‑1 (MQ=255) gCTGCGTGAGGCGGTCTGGATTTGGCTGCCAAGCAAATCGCGGCAGTGGCTAAGCAGGCTGGCGACGATAAGCCTTGACGt                                                                          <  1:774398/81‑1 (MQ=255)  cTGCGTGAGGCGGTCTGGATTTGGCTGCCAAGCGAATCGCGGCAGTGGCTAAGCAGGCTGGCGACGATAAGCCTTGACGtt                                                                         <  1:774402/81‑1 (MQ=255)  cTGCGTGAGGCGGTCTGGATTTGGCTGCCAAGCAAATCGCGGCAGTGGCTAAGCAGGCTGGCGACGATAAGCCTTGACGtt                                                                         <  1:774401/81‑1 (MQ=255)  cTGCGTGAGGCGGTCTGGATTTGGCTGCCAAGCAAATCGCGGCAGTGGCTAAGCAGGCTGGCGACGATAAGCCTTGACGtt                                                                         >  2:774459/1‑81 (MQ=255)  cTGCGTGAGGCGGTCTGGATTTGGCTGCCAAGCAAATCGCGGCAGTGGCTAAGCAGGCTGGCGACGATAAGCCTTGACGtt                                                                         <  2:774399/81‑1 (MQ=255)    gCGTGAGGCGGTCTGGATTTGGCTGCCAAGCAAATCGCGGCAGTGGCTAAGCAGGCTGGCGACGATAAGCCTTGACGTTtg                                                                       >  1:774445/1‑81 (MQ=255)     cGTGAGGCGGTCTGGATTTGGCTGCCAAGCAAATCGCGGCAGTGGCTAAGCAGGCTGGCGACGATAAGCCTTGACGttt                                                                        <  1:774403/79‑1 (MQ=255)      gTGAGGCGGTCTGGATTTGGCTGCCAAGCAAATCGCGGCAGTGGCTAAGCAGGCTGGCGACGATAAGCCTTGACGTTtgct                                                                     >  2:774461/1‑81 (MQ=255)      gTGAGGCGGTCTGGATTTGGCTGCCAAGCAAATCGCGGCAGTGGCTAAGCAGGCTGGCGACGATAAGCCTTGACGTTtgct                                                                     >  1:774457/1‑81 (MQ=255)        gAGGCGGTCTGGATTTGGCTGCCAAGCAAATCGCGGCAGTGGCTAAGCAGGCTGGCGACGATAAGCCTTGACGTTtgctgc                                                                   >  2:774453/1‑81 (MQ=255)              gTCTGGATTTGGCTGCCAAGCAAATCGCGGCAGTGGCTAAGCAGGCTGGCGACGATAAGCCTTGACGTTTGCTGCTCCTGc                                                             <  1:774404/81‑1 (MQ=255)                   gATTTGGCTGCCAAGCAAATCGCGGCAGTGGCTAAGCAGGCTGGCGACGATAAGCCTTGACGTTTGCTGCTCCTGCGGCtg                                                        >  2:774450/1‑81 (MQ=255)                   gATTTGGCTGCCAAGCAAATCGCGGCAGTGGCTAAGCAGGCTGGCGACGATAAGCCTTGACGTTTGCTGCTCCTGCGGCtg                                                        <  2:774404/81‑1 (MQ=255)                               aaGCAAATCGCGGCAGTGGCTAAGCAGGCTGGCGACGATAAGCCTTGACGTTTGCTGCTTCTGCGGCTGCATTTGCATTTc                                            <  2:774405/81‑1 (MQ=255)                               aaGCAAATCGCGGCAGTGGCTAAGCAGGCTGGCGACGATAAGCCTTGACGTTTGCTGCTCCTGCGGCTgcatttgcattt                                             >  1:774466/1‑80 (MQ=255)                                  cAAATCGCGGCAGTGGCTAAGCAGGCTGGCGACGATAAGCCTTGACGTTTGCTGCTCCTGCGGCTGCATTTGCATTTCAtt                                         <  2:774406/81‑1 (MQ=255)                                   aaaTCGCGGCAGTGGCTAAGCAGGCTGGCGACGATAAGCCTTGACGTTTGCTGCTCCTGCGGCTGCATTTGCATTTCAtt                                         >  1:774459/1‑80 (MQ=255)                                    aaTCGCGGCAGTGGCTAAGCAGGCTGGCGACGATAAGCCTTGACGTTTGCTGCTCCTGCGGCTGCATTTGCATTTCATTGa                                       <  2:774407/81‑1 (MQ=255)                                        gcgGCAGTGGCTAAGCAGGCTGGCGACGATAAGCCTTGACGTTTGCTGCTCCTGCGGCTGCATTTGCATTTCATTGAGCGt                                   <  2:774408/81‑1 (MQ=255)                                        gcgGCAGTGGCTAAGCAGGCTGGCGACGATAAGCCTTGACGTTTGCTGCTCCTGCGGCTGCATTTGCATTTCATTGAGCGt                                   >  1:774465/1‑81 (MQ=255)                                         cgGCAGTGGCTAAGCAGGCTGGCGACGATAAGCCTTGACGTTTGCTGCTCCTGCGGCTGCATTTGCATTTCATTGAGCGtt                                  >  2:774467/1‑81 (MQ=255)                                         cgGCAGTGGCTAAGCAGGCTGGCGACGATAAGCCTTGACGTTTGCTGCTCCTGCGGCTGCATTTGCATTTCATTGAGCGtt                                  >  2:774476/1‑81 (MQ=255)                                         cgGCAGTGGCTAAGCAGGCTGGCGACGATAAGCCTTGACGTTTGCTGCTCCTGCGGCTGCATTTGCATTTCATTGAGCGtt                                  <  2:774409/81‑1 (MQ=255)                                         cgGCAGTGGCTAAGCAGGCTGGCGACGATAAGCCTTGACGTTTGCTGCTCCTGCGGCTGCATTTGCATTTCATTGAGCGtt                                  <  2:774410/81‑1 (MQ=255)                                          ggCAGTGGCTAAGCAGGCTGGCGACGATAAGCCTTGACGTTTGCTGCTCCTGCGGCTGCATTTGCATTTCATTGAGCGttt                                 >  1:774463/1‑81 (MQ=255)                                            cAGTGGCTAAGCAGGCTGGCGACGATAAGCCTTGACGTTTGCTGCTCCTGCGGCTGCATTTGCATTTCATTGAGCGTTTGt                               <  2:774411/81‑1 (MQ=255)                                               tGGCTAAGCAGGCTGGCGACGATAAGCCTTGACGTTTGCTGCTCCTGCGGCTGCATTTGCATTTCATTGAGCGTTTGTAGc                            <  1:774413/81‑1 (MQ=255)                                                ggCTAAGCAGGCTGGCGACGATAAGCCTTGACGTTTGCTGCTCCTGCGGCTGCATTTGCATTTCATTGAGCGTTTGTAg                             >  2:774466/1‑79 (MQ=255)                                                     aGCAGGCTGGCGACGATAAGCCTTGACGTTTGCTGCTCCTGCGGCTGCATTTGCATTTCATTGAGCGTTTGTAGCAGaaa                       <  2:774414/80‑1 (MQ=255)                                                     aGCAGGCTGGCGACGATAAGCCTTGACGTTTGCTGCTCCTGCGGCTGCATTTGCATTTCATTGAGCGTTTGTAGCAGaa                        <  1:774414/79‑1 (MQ=255)                                                        aGGCTGGCGACGATAAGCCTTGACGTTTGCTGCTCCTGCGGCTGCATTTGCATTTCATTGAGCGTTTGTAGCAGAAAAGaa                   >  1:774482/1‑81 (MQ=255)                                                         ggCTGGCGACGATAAGCCTTGACGTTTGCTGCTCCTGCGGCTGCATTTGCATTTCATTGAGCGTTTGTAGCAGAAAAGAAc                  <  2:774415/81‑1 (MQ=255)                                                           cTGGCGACGATAAGCCTTGACGTTTGCTGCTCCTGCGGCTGCATTTGCATTTCATTGAGCGTTTGTAGCAGAAAAGAAcc                 >  2:774468/1‑80 (MQ=255)                                                           cTGGCGACGATAAGCCTTGACGTTTGCTGCTCCTGCGGCTGCATTTGCATTTCATTGAGCGTTTGTAGCAGAAAAGAACCt                <  1:774417/81‑1 (MQ=255)                                                               cgacgaTAAGCCTTGACGTTTGCTGCTCCTGCGGCTGCATTTGCATTTCATTGAGCGTTTGTAGCAGAAAAGAACCTATGc            <  1:774418/81‑1 (MQ=255)                                                                gacgaTAAGCCTTGACGTTTGCTGCTCCTGCGGCTGCATTTGCATTTCATTGAGCGTTTGTAGCAGAAAAGAACCTATGCg           >  2:774484/1‑81 (MQ=255)                                                                   gaTAAGCCTTGACGTTTGCTGCTCCTGCGGCTGCATTTGCATTTCATTGAGCGTTTGTAGCAGAAAAGAACCTATGCGTgg        <  1:774419/81‑1 (MQ=255)                                                                   gaTAAGCCTTGACGTTTGCTGCTCCTGCGGCTGCATTTGCATTTCATTGAGCGTTTGTAGCAGAAAAGAACCTATGCGTgg        >  2:774471/1‑81 (MQ=255)                                                                    aTAAGCCTTGACGTTTGCTGCTCCTGCGGCTGCATTTGCATTTCATTGAGCGTTTGTAGCAGAAAAGAACCTATGCGTggg       >  2:774460/1‑81 (MQ=255)                                                                     tAAGCCTTGACGTTTGCTGCTCCTGCGGCTGCATTTGCATTTCATTGAGCGTTTGTAGCAGAAAAGAACCTATGCGTGGGc      <  2:774419/81‑1 (MQ=255)                                                                       aGCCTTGACGTTTGCTGCTCCCGCGGCTGCATTTGCATTTCATTGAGCGTTTGTAGCAGAAAAGAACCTATGCGTGGGCcg    >  1:774453/1‑81 (MQ=255)                                                                         ccTTGACGTTTGCTGCTCCTGCGGCTGCATTTGCATTTCATTGAGCGTTTGTAGCAGAAAAGAACCTATGCGTGGGCcgcg  >  2:774469/1‑81 (MQ=255)                                                                              |                                                                            GCTGCGTGAGGCGGTCTGGATTTGGCTGCCAAGCAAATCGCGGCAGTGGCTAAGCAGGCTGGCGACGATAAGCCTTGCCGTTTGCTGCTCCTGCGGCTGCATTTGCATTTCATTGAGCGTTTGTAGCAGAAAAGAACCTATGCGTGGGCCGCG  >  NC_000913/4147817‑4147969

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑