Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,156,840	A→G	intergenic (+51/‑10)	argB → / → argH	acetylglutamate kinase/argininosuccinate lyase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,156,840	0	A	G	100.0%	134.0 / NA	39	intergenic (+51/‑10)	argB/argH	acetylglutamate kinase/argininosuccinate lyase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (22/17);  total (22/17)

CCGATGGGTACGCGGATTTTAGCTTAAGTTTTGTTGGCCGGAGGCGCAGCTTTCCGGCATTGAATTTCAAAATAAGGAAACAGAGTTATGGCACTTTGGGGCGGGCGTTTTACCCAGGCAGCAGATCAACGGTTCAAACAATTCAACGACTCAC  >  NC_000913/4156763‑4156916                                                                              |                                                                             ccGATGGGTACGCGGATTTTAGCTTAAGTTTTGTTGGCCGGAGGCGCAGCTTTCCGGCATTGAATTTCAAAATAAGGgaa                                                                            >  1:776836/1‑80 (MQ=255)  cGATGGGTACGCGGATTTTAGCTTAAGTTTTGTTGGCCGGAGGCGCAGCTTTCCGGCATTGAATTTCAAAATAAGGGAAcc                                                                          >  1:776820/1‑80 (MQ=255)   gATGGGTACGCGGATTTTAGCTTAAGTTTTGTTGGCCGGAGGCGCAGCTTTCCGGCATTGAATTTCAAAATAAGGgaa                                                                            >  2:776808/1‑78 (MQ=255)   gATGGGTACGCGGATTTTAGCTTAAGTTTTGTTGGCCGGAGGCGCAGCTTTCCGGCATTGAATTTCAAAATAAGGgaa                                                                            >  1:776838/1‑78 (MQ=255)   gATGGGTACGCGGATTTTAGCTTAAGTTTTGTTGGCCGGAGGCGCAGCTTTCCGGCATTGAATTTCAAAATAAGGgaa                                                                            >  1:776830/1‑78 (MQ=255)       ggTACGCGGATTTTAGCTTAAGTTTTGTTGGCCGGAGGCGCAGCTTTCCGGCATTGAATTTCAAAATAAGGGAACAGAGt                                                                      >  2:776843/1‑80 (MQ=255)       ggTACGCGGATTTTAGCTTAAGTTTTGTTGGCCGGAGGCGCAGCTTTCCGGCATTGAATTTCAAAATAAGGGAACAGAGt                                                                      >  1:776831/1‑80 (MQ=255)           cgcgGATTTTAGCTTAAGTTTTGTTGGCCGGAGGCGCAGCTTTCCGGCATTGAATTTCAAAATAAGGGAACAGAGTTATgg                                                                 >  1:776841/1‑81 (MQ=255)           cgcgGATTTTAGCTTAAGTTTTGTTGGCCGGAGGCGCAGCTTTCCGGCATTGAATTTCAAAATAAGGGAACAGAGTTATg                                                                  <  1:776772/80‑1 (MQ=255)              ggATTTTAGCTTAAGTTTTGTTGGCCGGAGGCGCAGCTTTCCGGCATTGAATTTCAAAATAAGGGAACAGAGTTATGGCAc                                                              >  2:776816/1‑81 (MQ=255)               gATTTTAGCTTAAGTTTTGTTGGCCGGAGGCGCAGCTTTCCGGCATTGAATTTCAAAATAAGGGAACAGAGTTATGGCACt                                                             >  1:776812/1‑81 (MQ=255)                aTTTTAGCTTAAGTTTTGTTGGCCGGAGGCGCAGCTTTCCGGCATTGAATTTCAAAATAAGGGAACAGAGTTATGGCACtt                                                            <  1:776773/81‑1 (MQ=255)                 ttttAGCTTAAGTTTTGTTGGCCGGAGGCGCAGCTTTCCGGCATTGAATTTCAAAATAAGGGAACAGAGTTATGGCACttt                                                           <  1:776774/81‑1 (MQ=255)                 ttttAGCTTAAGTTTTGTTGGCCGGAGGCGCAGCTTTCCGGCATTGAATTTCAAAATAAGGGAACAGAGTTATGGCACttt                                                           <  2:776774/81‑1 (MQ=255)                  tttAGCTTAAGTTTTGTTGGCCGGAGGCGCAGCTTTCCGGCATTGAATTTCAAAATAAGGGAACAGAGTTATGGCACTTTg                                                          >  2:776836/1‑81 (MQ=255)                      gCTTAAGTTTTGTTGGCCGGAGGCGCAGCTTTCCGGCATTGAATTTCAAAATAAGGGAACAGAGTTATGGCACTTTgggg                                                       <  2:776775/80‑1 (MQ=255)                           aGTTTTGTTGGCCGGAGGCGCAGCTTTCCGGCATTGAATTTCAAAATAAGGGAACAGAGTTATGGCACTTTGgggcgggc                                                  <  1:776777/80‑1 (MQ=255)                           aGTTTTGTTGGCCGGAGGCGCAGCTTTCCGGCATTGAATTTCAAAATAAGGGAACAGAGTTATGGCACTTTGGggcgggcg                                                 >  1:776814/1‑81 (MQ=255)                               ttgttgGCCGGAGGCGCAGCTTTCCGGCATTGAATTTCAAAATAAGGGAACAGAGTTATGGCACTTTGGGGCGGGCGttt                                              >  2:776817/1‑80 (MQ=255)                                 gttgGCCGGAGGCGCAGCTTTCCGGCATTGAATTTCAAAATAAGGGAACAGAGTTATGGCACTTTGGGGCGGGCGTTTTAc                                           >  2:776834/1‑81 (MQ=255)                                  ttgGCCGGAGGCGCAGCTTTCCGGCATTGAATTTCAAAATAAGGGAACAGAGTTATGGCACTTTGGGGCGGGCGTTTTAcc                                          >  2:776824/1‑81 (MQ=255)                                    ggCCGGAGGCGCAGCTTTCCGGCATTGAATTTCAAAATAAGGGAACAGAGTTATGGCACTTTGGGGCGGGCGTTTTAccc                                         <  2:776777/80‑1 (MQ=255)                                    ggCCGGAGGCGCAGCTTTCCGGCATTGAATTTCAAAATAAGGGAACAGAGTTATGGCACTTTGGGGCGGGCGTTTTAccc                                         <  2:776778/80‑1 (MQ=255)                                     gCCGGAGGCGCAGCTTTCCGGCATTGAATTTCAAAATAAGGGAACAGAGTTATGGCACTTTGGGGCGGGCGTTTTACCCa                                        <  2:776779/80‑1 (MQ=255)                                        ggAGGCGCAGCTTTCCGGCATTGAATTTCAAAATAAGGGAACAGAGTTATGGCACTTTGGGGCGGGCGTTTTACCCAgg                                      >  2:776823/1‑79 (MQ=255)                                        ggAGGCGCAGCTTTCCGGCATTGAATTTCAAAATAAGGGAACAGAGTTATGGCACTTTGGGGCGGGCGTTTTACCCAgg                                      >  2:776827/1‑79 (MQ=255)                                          aGGCGCAGCTTTCCGGCATTGAATTTCAAAATAAGGGAACAGAGTTATGGCACTTTGGGGCGGGCGTTTTACCCAGgcagc                                  <  1:776781/81‑1 (MQ=255)                                          aGGCGCAGCTTTCCGGCATTGAATTTCAAAATAAGGGAACAGAGTTATGGCACTTTGGGGCGGGCGTTTTACCCAGgcag                                   >  2:776831/1‑80 (MQ=255)                                              gcAGCTTTCCGGCATTGAATTTCAAAATAAGGGAACAGAGTTATGGCACTTTGGGGCGGGCGTTTTACCCAGgcag                                   >  1:776833/1‑76 (MQ=255)                                                aGCTTTCCGGCATTGAATTTCAAAATAAGGGAACAGAGTTATGGCACTTTGGGGCGGGCGTTTTACCCAGGCAGCAGATc                             >  1:776821/1‑80 (MQ=255)                                                    ttCCGGCATTGAATTTCAAAATAAGGGAACAGAGTTATGGCACTTTGGGGCGGGCGTTTTACCCAGGCAGCAGATCAACgg                        >  1:776822/1‑81 (MQ=255)                                                     tCCGGCATTGAATTTCAAAATAAGGGAACAGAGTTATGGCACTTTGGGGCGGGCGTTTTACCCAGGCAGCAGATCAACGGt                       >  1:776842/1‑81 (MQ=255)                                                     tCCGGCATTGAATTTCAAAATAAGGGAACAGAGTTATGGCACTTTGGGGCGGGCGTTTTACCCAGGCAGCAGATCAACGGt                       >  2:776842/1‑81 (MQ=255)                                                          cATTGAATTTCAAAATAAGGGAACAGAGTTATGGCACTTTGGGGCGGGCGTTTTACCCAGGCAGCAGATCAACGGTTCaaa                  <  1:776782/81‑1 (MQ=255)                                                           aTTGAATTTCAAAATAAGGGAACAGAGTTATGGCACTTTGGGGCGGGCGTTTTACCCAGGCAGCAGATCAACGGTTCaaa                  >  1:776851/1‑80 (MQ=255)                                                           aTTGAATTTCAAAATAAGGGAACAGAGTTATGACACTTTGGGGCGGGCGTTTTACCCAGGCAGCAGATCAACGGTTCAAAc                 <  1:776783/81‑1 (MQ=255)                                                             tGAATTTCAAAATAAGGGAACAGAGTTATGGCACTTTGGGGCGGGCGTTTTACCCAGGCAGCAGATCAACGGTTCAAACaa               >  1:776834/1‑81 (MQ=255)                                                               aaTTTCAAAATAAGGGAACAGAGTTATGGCACTTTGGGGCGGGCGTTTTACCCAGGCAGCAGATCAACGGTTCAAACAAtt             >  2:776846/1‑81 (MQ=255)                                                               aaTTTCAAAATAAGGGAACAGAGTTATGGCACTTTGGGGCGGGCGTTTTACCCAGGCAGCAGATCAACGGTTCAAACAAt              <  2:776783/80‑1 (MQ=255)                                                                aTTTCAAAATAAGGGAACAGAGTTATGGCACTTTGGGGCGGGCGTTTTACCCAGGCAGCAGATCAACGGTTCAAACAATTc            <  2:776784/81‑1 (MQ=255)                                                                   tCAAAATAAGGGAACAGAGTTATGGCACTTTGGTGCGGGCGTTTTACCCAGGCAGCAGATCAACGGTTCAAACAATTCaa          <  2:776785/80‑1 (MQ=255)                                                                      aaaTAAGGGAACAGAGTTATGGCACTTTGGGGCGGGCGTTTTACCCAGGCAGCAGATCAACGGTTCAAACAATTCAACGaa      <  1:776787/81‑2 (MQ=255)                                                                           aGGGAACAGAGTTATGGCACTTTGGGGCGGGCGTTTTACCCAGGCAGCAGATCAACGGTTCAAACAATTCAACGACTCAc  <  2:776787/80‑1 (MQ=255)                                                                              |                                                                             CCGATGGGTACGCGGATTTTAGCTTAAGTTTTGTTGGCCGGAGGCGCAGCTTTCCGGCATTGAATTTCAAAATAAGGAAACAGAGTTATGGCACTTTGGGGCGGGCGTTTTACCCAGGCAGCAGATCAACGGTTCAAACAATTCAACGACTCAC  >  NC_000913/4156763‑4156916

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑