Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,221,347	T→C	I381V (ATT→GTT)	arpA ←	regulator of acetyl CoA synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,221,347	0	T	C	100.0%	48.0 / NA	15	I381V (ATT→GTT)	arpA	regulator of acetyl CoA synthetase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (3/12);  total (3/12)

ACAATGAATTGAAAATAGTCTCAACGATATCCGCATTACCATAATTTATAGCCAAATATAAACCTGGCAGGTTAATACTATTATAGGCAGTCAACATTTCTGTTCTATGTAGTTCTGGCATTTTCTGGAG  >  NC_000913/4221272‑4221401                                                                            |                                                       aCAATGAATTGAAAATAGTCTCAACGATATCCGCATTACCATAATTTATAGCCAAATATAAACCTGGCAGGTTAacac                                                      <  2:794074/78‑1 (MQ=255) aCAATGAATTGAAAATAGTCTCAACGATATCCGCATTACCATAATTTATAGCCAAATATAAACCTGGCAGGTTAACACta                                                    <  1:794076/80‑1 (MQ=255)       aaTTGAAAATAGTCTCAACGATATCCGCATTACCATAATTTATAGCCAAATATAAACCTGGCAGGTTAACACTATTATAg                                              <  1:794077/80‑1 (MQ=255)           gAAAATAGTCTCAACGATATCCGCATTACCATAATTTATAGCCAAATATAAACCTGGCAGGTTAACACTATTATAGGCAg                                          <  2:794077/80‑1 (MQ=255)            aaaaTAGTCTCAACGATATCCGCATTACCATAATTTATAGCCAAATATAAACCTGGCAGGTTAACACTATTATAGGCAGTc                                        <  1:794079/81‑1 (MQ=255)                      cAACGATATCCGCATTACCATAATTTATAGCCAAATATAAACCTGGCAGGTTAACACTATTATAGGCAGTCAACATTTCTg                              >  2:794118/1‑81 (MQ=255)                       aaCGATATCCGCATTACCATAATTTATAGCCAAATATAAACCTGGCAGGTTAACACTATTATAGGCAGTCAACATTTCt                               <  2:794079/79‑1 (MQ=255)                       aaCGATATCCGCATTACCATAATTTATAGCCAAATATAAACCTGGCAGGTTAACACTATTATAGGCAGTCAACATTTCTg                              <  2:794080/80‑1 (MQ=255)                         cGATATCCGCATTACCATAATTTATAGCCAAATATAAACCTGGCAGGTTAACACTATTATAGGCAGTCAACATTTCTGTTc                           <  2:794081/81‑1 (MQ=255)                          gATATCCGCATTACCATAATTTATAGCCAAATATAAACCTGGCAGGTTAACACTATTATAGGCAGTCAACATTTCTGTTCt                          <  2:794082/81‑1 (MQ=255)                                    ttACCATAATTTATAGCCAAATATAAACCTGGCAGGTTAACACTATTATAGGCAGTCAACATTTCTGTTCTATGTAGTTCt                <  2:794083/81‑1 (MQ=255)                                            aTTTATAGCCAAATATAAACCTGGCAGGTTAACACTATTATAGGCAGTCAACATTTCTGTTCTATGTAGTTCTGGCAttt         <  1:794086/80‑1 (MQ=255)                                            aTTTATAGCCAAATATAAACCTGGCAGGTTAACACTATTATAGGCAGTCAACATTTCTGTTCTATGTAGTTCTGGCAttt         <  2:794084/80‑1 (MQ=255)                                             tttATAGCCAAATATAAACCTGGCAGGTTAACACTATTATAGGCAGTCAACATTTCTGTTCTATGTAGTTCTGGCATTTTc       <  2:794086/81‑1 (MQ=255)                                              ttATAGCCAAATATAAACCTGGCAGGTTAACACTATTATAGGCAGTCAACATTTCTGTTCTATGTAGTTCTGGCATTTTCt      >  2:794116/1‑81 (MQ=255)                                                  aGCCAAATATAAACCTGGCAGGTTAACACTATTATAGGCAGTCAACATTTCTGTTCTATGTAGTTCTGGCATTTTCTGGAg  >  2:794110/1‑81 (MQ=255)                                                                            |                                                       ACAATGAATTGAAAATAGTCTCAACGATATCCGCATTACCATAATTTATAGCCAAATATAAACCTGGCAGGTTAATACTATTATAGGCAGTCAACATTTCTGTTCTATGTAGTTCTGGCATTTTCTGGAG  >  NC_000913/4221272‑4221401

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑