Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,222,138	G→A	T117I (ACT→ATT)	arpA ←	regulator of acetyl CoA synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,222,138	0	G	A	100.0%	87.6 / NA	25	T117I (ACT→ATT)	arpA	regulator of acetyl CoA synthetase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (10/15);  total (10/15)

CATTGACCATTAAGTTTAATTCTTGGGAATTCTTTTTCTTTTCTATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAGTGTCTGAGCGTTGAGGTAAAATTTCCATTAGGTATTTTTCAAATATTGGGGCAATGGTTTGAGTAAGTGTCGT  >  NC_000913/4222060‑4222211                                                                               |                                                                          cATTGACCATTAAGTTTAGTTCTTGGGAATTCTTTTTCTTTTCTATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAatg                                                                         <  1:794214/81‑1 (MQ=255)  aTTGACCATTAAGTTTAATTCTTGGGAATTCTTTTTCTTTTCTATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAatgt                                                                        <  1:794215/81‑1 (MQ=255)    tGACCATTAAGTTTAATTCTTGGGAATTCTTTTTCTTTTCTATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAATGTCt                                                                      >  2:794249/1‑81 (MQ=255)          ttAAGTTTAATTCTTGGGAATTCTTTTTCTTTTCTATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAATGTCTGAGCGt                                                                <  1:794216/81‑1 (MQ=255)            aaGTTTAATTCTTGGGAATTCTTTTTCTTTTCTATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAATGTCTGAGCGtt                                                               >  1:794257/1‑80 (MQ=255)            aaGTTTAATTCTTGGGAATTCTTTTTCTTTTCTATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAATGTCTGAGCGtt                                                               >  2:794252/1‑80 (MQ=255)            aaGTTTAATTCTTGGGAATTCTTTTTCTTTTCTATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAATGTCTGAGCGtt                                                               <  1:794217/80‑1 (MQ=255)             aGTTTAATTCTTGGGAATTCTTTTTCTTTTCTATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAATGTCTGAGCGTTg                                                              <  2:794217/80‑1 (MQ=255)                      cTTGGGAATTCTTTTTCTTTTCTATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAATGTCTGAGCGTTGAGGTAAAAt                                                     <  1:794220/80‑1 (MQ=255)                      cTTGGGAATTCTTTTTCTTTTCTATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAATGTCTGAGCGTTGAGGTAAAAt                                                     <  2:794218/80‑1 (MQ=255)                      cTTGGGAATTCTTTTTCTTTTCTATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAACGTCTGAGCGTTGAGGTAAAAtt                                                    >  1:794248/1‑81 (MQ=255)                       ttGGGAATTCTTTTTCTTTTCTATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAATGTCTGAGCGTTGAGGTAAAAttt                                                   >  2:794245/1‑81 (MQ=255)                          ggAATTCTTTATCTTTTCTATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAATGTCTGAGCGTTGAGGTAAAATTTcc                                                 <  1:794221/80‑1 (MQ=255)                           gAATTCTTTTTCTTTTCTATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAATGTCTGAGCGTTGAGGTAAAATTTCCAt                                               <  2:794221/81‑1 (MQ=255)                             aTTCTTTTTCTTTTCTATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAATGTCTGAGCGTTGAGGTAAAATTTCCATTa                                             <  1:794223/81‑1 (MQ=255)                                         ttctATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAATGTCTGAGCGTTGAGGTAAAATTTCCATTAGGTATTTTTc                                   <  1:794224/79‑1 (MQ=255)                                             aTCTGATTTTAGGTTTAATTCTTGTTTATCAAGAATGTCTGAGCGTTGAGGTAAAATTTCCATTAGGTATTTTTCAAatat                             >  1:794281/1‑81 (MQ=255)                                              tCTGATTTTAGGTTTAATTCTTGTTTATCAAGAATGTCTGAGCGTTGAGGTAAAATTTCCATTAGGTATTTTTCAAata                              <  1:794225/79‑1 (MQ=255)                                              tCTGATTTTAGGTTTAATTCTTGTTTATCAAGAATGTCTGAGCGTTGAGGTAAAATTTCCATTAGGTATTTTTCAAat                               <  1:794226/78‑1 (MQ=255)                                                     ttAGGTTTAATTCTTGTTTATCAAGAATGTCTGAGCGTTGAGGTAAAATTTCCATTAGGTATTTTTCAAATATTGGGGCaa                     >  2:794239/1‑81 (MQ=255)                                                               ttCTTGTTTATCAAGAATGTCTGAGCGTTGAGGTAAAATTTCCATTAGGTATTTTTCAAATATTGGGGCAATGGTTTGAg            >  2:794255/1‑80 (MQ=255)                                                                tCTTGTTTATCAAGAATGTCTGAGCGTTGAGGTAAAATTTCCATTAGGTATTTTTCAAATATTGGGGCAATGGTTTGAGt           >  2:794250/1‑80 (MQ=255)                                                                    gTTTATCAAGAATGTCTGAGCGTTGAGGTAAAATTTCCATTAGGTATTTTTCAAATATTGGGGCAATGGTTTGAGTAAg        <  1:794227/79‑1 (MQ=255)                                                                     tttATCAAGAATGTCTGAGCGTTGAGGTAAAATTTCCATTAGGTATTTTTCAAATATTGGGGCAATGGTTTGAGTAAgtgt     >  2:794260/1‑81 (MQ=255)                                                                       tATCAAGAATGTCTGAGCGTTGAGGTAAAATTTCCATTAGGTATTTTTCAAATATTGGGGCAATGGTTTGAGTAAGTGTc    <  1:794228/80‑1 (MQ=255)                                                                        aTCAAGAATGTCTGAGCGTTGAGGTAAAATTTCCATTAGGTATTTTTCAAATATTGGGGCAATGGTTTGAGTAAGTGTCg   <  2:794228/80‑1 (MQ=255)                                                                         tCAAGAATGTCTGAGCGTTGAGGTAAAATTACCATTAGGTATTTTTCAAATATTGGGGCAATGGTTTGAGTAAGTGTCgt  <  2:794229/80‑1 (MQ=255)                                                                               |                                                                          CATTGACCATTAAGTTTAATTCTTGGGAATTCTTTTTCTTTTCTATCTGATTTTAGGTTTAATTCTTGTTTATCAAGAGTGTCTGAGCGTTGAGGTAAAATTTCCATTAGGTATTTTTCAAATATTGGGGCAATGGTTTGAGTAAGTGTCGT  >  NC_000913/4222060‑4222211

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑