Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,232,891	C→T	V115I (GTC→ATC)	lysC ←	aspartate kinase III

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,232,891	0	C	T	100.0%	199.2 / NA	56	V115I (GTC→ATC)	lysC	aspartate kinase III
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (31/25);  total (31/25)

AAACCACTGTGCCTGAACATCGCGTTCGCGCAGGATCTCAACAAACAGCAGGGTCGACATCAGCTCGCCGTGGCTGACCAGCTCATCTGTCAGCGCCGGAGACGTTGCCAGCGCCGCCGCTTCTGCCAGAACAGTAATGTTCTCCAGCAGACGT  >  NC_000913/4232814‑4232967                                                                              |                                                                             aaaCCACTGTGCCTGAACATCGCGTTCGCGCAGGATCTCAACAAACAGCAGGGTCGACATCAGCTCGCCGTGGCTGATCa                                                                            <  2:797046/80‑1 (MQ=255) aaaCCACTGTGCCTGAACATCGCGTTCGCGCAGGATCTCAACAAACAGCAGGGTCGACATCAGCTCGCCGTGGCTGATCAg                                                                           >  2:797138/1‑81 (MQ=255) aaaCCACTGTGCCTGAACATCGCGTTCGCGCAGGATCTCAACAAACAGCAGGGTCGACATCAGCTCGCCGTGGCTGATCAg                                                                           >  2:797115/1‑81 (MQ=255)  aaCCACTGTGCCTGAACATCGCGTTCGCGCAGGATCTCAACAAACAGCAGGGTCGACATCAGCTCGCCGTGGCTGATCAGc                                                                          >  1:797133/1‑81 (MQ=255)       cTGTGCCTGAACATCGCGTTCGCGCAGGATCTCAACAAACAGCAGGGTCGACATCAGCTCGCCGTGGCTGATCAGCTCATc                                                                     >  1:797120/1‑81 (MQ=255)        tgtgCCTGAACATCGCGTTCGCGCAGGATCTCAACAAACAGCAGGGTCGACATCAGCTCGCCGTGGCTGATCAGCTCATCt                                                                    >  1:797138/1‑81 (MQ=255)        tgtgCCTGAACATCGCGTTCGCGCAGGATCTCAACAAACAGCAGGGTCGACATCAGCTCGCCGTGGCTGATCAGCTCATCt                                                                    >  1:797136/1‑81 (MQ=255)          tgCCTGAACATCGCGTTCGCGCAGGATCTCAACAAACAGCAGGGTCGACATCAGCTCGCCGTGGCTGATCAGCTCATCt                                                                    <  1:797049/79‑1 (MQ=255)          tgCCTGAACATCGCGTTCGCGCAGGATCTCAACAAACAGCAGGGTCGACATCAGCTCGCCGTGGCTGATCAGCTCATCTGt                                                                  <  2:797047/81‑1 (MQ=255)           gCCTGAACATCGCGTTCGCGCAGGATCTCAACAAACAGCAGGGTCGACATCAGCTCGCCGTGGCTGATCAGCTCATCTGt                                                                  >  2:797118/1‑80 (MQ=255)            ccTGAACATCGCGTTCGCGCAGGATCTCAACAAACAGCAGGGTCGACATCAGCTCGCCTTGGCTGATCAGCTCATCTGt                                                                  >  1:797144/1‑79 (MQ=255)             cTGAACATCGCGTTCGCGCAGGATCTCAACAAACAGCAGGGTCGACATCAGCTCGCCGTGGCTGATCAGCTCATCTGTCAg                                                               <  1:797050/81‑1 (MQ=255)              tGAACATCGCGTTCGCGCAGGATCTCAACAAACAGCAGGGTCGACATCAGCTCGCCGTGGCTGATCAGCTCATCTGTCAgc                                                              >  2:797112/1‑81 (MQ=255)               gAACATCGCGTTCGCGCAGGATCTCAACAAACAGCAGGGTCGACATCAGCTCGCCGTGGCTGATCAGCTCATCTGTCAgcg                                                             >  1:797121/1‑81 (MQ=255)                 aCATCGCGTTCGCGCAGGATCTCAACAAACAGCAGGGTCGACATCAGCTCGCCGTGGCTGATCAGCTCATCTGTCAGCGcc                                                           <  2:797050/81‑1 (MQ=255)                       cgTTCGCGCAGGATCTCAACAAACAGCAGGGTCGACATCAGCTCGCCGTGGCTGATCAGCTCATCTGTCAGCGCCGGAGAc                                                     <  2:797051/81‑1 (MQ=255)                        gTTCGCGCAGGATCTCAACAAACAGCAGGGTCGACATCAGCTCGCCGTGGCTGATCAGCTCATCTGTCAGCGCCGGAGACg                                                    >  2:797134/1‑81 (MQ=255)                         ttCGCGCAGGATCTCAACAAACAGCAGGGTCGACATCAGCTCGCCGTGGCTGATCAGCTCATCTGTCAGCGCCGGAGACGt                                                   >  2:797108/1‑81 (MQ=255)                          tCGCGCAGGATCTCAACAAACAGCAGGGTCGACATCAGCTCGCCGTGGCTGATCAGCTCATCTGTCAGCGCCGGAGACGtt                                                  >  1:797126/1‑81 (MQ=255)                           cgcgCAGGATCTCAACAAACAGCAGGGTCGACATCAGCTCGCCGTGGCTGATCAGCTCATCTGTCAGCGCCGGAGACGtt                                                  <  1:797053/80‑1 (MQ=255)                            gcgcAGGATCTCAACAAACAGCAGGGTCGACATCAGCTCGCCGTGGCTGATCAGCTCATCTGTCAGCGCCGGAGACGTTg                                                 <  1:797054/80‑1 (MQ=255)                              gcAGGATCTCAACAAACAGCAGGGTCGACATCAGCTCGCCGTGGCTGATCAGCTCATCTGTCAGCGCCGGAGACGTTGc                                                >  1:797128/1‑79 (MQ=255)                              gcAGGATCTCAACAAACAGCAGGGTCGACATCAGCTCGCCGTGGCTGATCAGCTCATCTGTCAGCGCCGGAGACGTTGc                                                >  1:797158/1‑79 (MQ=255)                              gcAGGATCTCAACAAACAGCAGGGTCGACATCAGCTCGCCGTGGCTGATCAGCTCATCTGTCAGCGCCGGAGACGTTGCCa                                              <  1:797055/81‑1 (MQ=255)                                     ctcAACAAACAGCAGGGTCGACATCAGCTCGCCGTGGCTGATCAGCTCATCTGTCAGCGCCGGAGACGTTGCCAGcgccgc                                       >  1:797131/1‑81 (MQ=255)                                       caacaaACAGCAGGGTCGACATCAGCTCGCCGTGGCTGATCAGCTCATCTGTCAGCGCCGGAGACGTTGCCAGcgccgccg                                     >  2:797121/1‑81 (MQ=255)                                       caacaaACAGCAGGGTCGACATCAGCTCGCCGTGGCTGATCAGCTCATCTGTCAGCGCCGGAGACGTTGCCAGcgccgccg                                     >  2:797122/1‑81 (MQ=255)                                       caacaaACAGCAGGGTCGACATCAGCTCGCCGTGGCTGATCAGCTCATCTGTCAGCGCCGGAGACGTTGCCAGcgccgccg                                     >  1:797140/1‑81 (MQ=255)                                        aacaaacaGCAGGGTCGACATCAGCTCGCCGTGGCTGATCAGCTCATCTGTCAGCGCCGGAGACGTTGCCAGcgccgccg                                     <  1:797057/80‑1 (MQ=255)                                        aacaaacaGCAGGGTCGACATCAGCTCGCCGTGGCTGATCAGCTCATCTGTCAGCGCCGGAGACGTTGCCAGcgccgcc                                      <  1:797056/79‑1 (MQ=255)                                           aaacaGCAGGGTCGACATCAGCTCGCCGTGGCTGATCAGCTCATCTGTCAGCGCCGGAGACGTTGCCAGCGCCGCCGCTTc                                 <  1:797058/81‑1 (MQ=255)                                           aaacaGCAGGGTCGACATCAGCTCGCCGTGGCTGATCAGCTCATCTGTCAGCGCCGGAGACGTTGCCAGCGCCGCCGCTTc                                 >  2:797155/1‑81 (MQ=255)                                            aacaGCAGGGTCGACATCAGCTCGCCGTGGCTGATCAGCTCATCTGTCAGCGCCGGAGACGTTGCCAGCGCCGCCGCTTCt                                <  2:797058/81‑1 (MQ=255)                                            aacaGCAGGGTCGACATCAGCTCGCCGTGGCTGATCAGCTCATCTGTCAGCGCCGGAGACGTTGCCAGCGCCGCCGCTTCt                                <  1:797060/81‑1 (MQ=255)                                            aacaGCAGGGTCGACATCAGCTCGCCGTGGCTGATCAGCTCATCTGTCAGCGCCGGAGACGTTGCCAGCGCCGCCGCTTCt                                <  1:797061/81‑1 (MQ=255)                                               agcagGGTCGACATCAGCTCGCCGTGGCTGATCAGCTCATCTGTCAGCGCCGGAGACGTTGCCAGCGCCGCCGCTTCTGcc                             <  2:797061/81‑1 (MQ=255)                                                 cagGGTCGACATCAGCTCGCCGTGGCTGATCAGCTCATCTGTCAGCGCCGGAGACGTTGCCAGCGCCGCCGCTTCTGc                              >  2:797140/1‑78 (MQ=255)                                                   gggTCGACATCAGCTCGCCGTGGCTGATCAGCTCATCTGTCAGCGCCGGAGACGTTGCCAGCGCCGCCGCTTCTGCCAGaa                         >  1:797124/1‑81 (MQ=255)                                                    ggTCGACATCAGCTCGCCGTGGCTGATCAGCTCATCTGTCAGCGCCGGAGACGTTGCCAGCGCCGCCGCTTCTGCCAGAAc                        <  2:797062/81‑1 (MQ=255)                                                        gACATCAGCTCGCCGTGGCTGATCAGCTCATCTGTCAGCGCCGGAGACGTTGCCAGCGCCGCCGCTTCTGCCAGAACAGt                     >  2:797151/1‑80 (MQ=255)                                                        gACATCAGCTCGCCGTGGCTGATCAGCTCATCTGTCAGCGCCGGAGACGCTGCCAGCGCCGCCGCTTCTGCCAGAACAGt                     >  2:797147/1‑80 (MQ=255)                                                           aTCAGCTCGCCGTGGCTGATCAGCTCATCTGTCAGCGCCGGAGACGTTGCCAGCGCCGCCGCTTCTGCCAGAACAGTAATg                 <  1:797064/81‑1 (MQ=255)                                                           aTCAGCTCGCCGTGGCTGATCAGCTCATCTGTCAGCGCCGGAGACGTTGCCAGCGCCGCCGCTTCTGCCAGAACAGTAATg                 >  2:797152/1‑81 (MQ=255)                                                               gCTCGCCGTGGCTGATCAGCTCATCTGTCAGCGCCGGAGATGTTGCCAGCGCCGCCGCTTCTGCCAGAACAGTAATGTtct             <  2:797064/81‑1 (MQ=255)                                                               gCTCGCCGTGGCTGATCAGCTCATCTGTCAGCGCCGGAGACGTTGCCAGCGCCGCCGCTTCTGCCAGAACAGTAATGTtct             >  2:797149/1‑81 (MQ=255)                                                                cTCGCCGTGGCTGATCAGCTCATCTGTCAGCGCCGGAGACGTTGCCAGCGCCGCCGCTTCTGCCAGAACAGTAATGTtctc            >  2:797154/1‑81 (MQ=255)                                                                   gCCGTGGCTGATCAGCTCATCTGTCAGCGCCGGAGACGTTGCCAGCGCCGCCGCTTCTGCCAGAACAGTAATGTTCTCcag         <  2:797065/81‑1 (MQ=255)                                                                     cGTGGCTGATCAGCTCATCTGTCAGCGCCGGAGACGTTGCCAGCGCCGCCGCTTCTGCCAGAACAGTAATGTTCTCcag         >  2:797141/1‑79 (MQ=255)                                                                      gTGGCTGATCAGCTCATCTGTCAGCGCCGGAGACGTTGCCAGCGCCGCCGCTTCTGCCAGAACAGTAATGTTCTCcagcag      <  1:797067/81‑1 (MQ=255)                                                                       tGGCTGATCAGCTCATCTGTCAGCGCCGGAGACGTTGCCAGCGCCGCCGCTTCTGCCAGAACAGTAATGTTCTCCAGCAGa     >  2:797133/1‑81 (MQ=255)                                                                         gCTGATCAGCTCATCTGTCAGCGCCGGAGACGTTGCCAGCGCCGCCGCTTCTGCCAGAACAGTAATGTTCTCCAGCAGAc    <  2:797069/80‑1 (MQ=255)                                                                         gCTGATCAGCTCATCTGTCAGCGCCGGAGACGTTGCCAGCGCCGCCGCTTCTGCCAGAACAGTAATGTTCTCCAGCAGAc    <  1:797068/80‑1 (MQ=255)                                                                         gCTGATCAGCTCATCTGTCAGCGCCGGAGACGTTGCCAGCGCCGCCGCTTCTGCCAGAACAGTAATGTTCTCCAGCAGAc    <  1:797069/80‑1 (MQ=255)                                                                         gCTGATCAGCTCATCTGTCAGCGCCGGAGACGTTGCCAGCGCCGCCGCTTCTGCCAGAACAGTAATGTTCTCCAGCAGACg   <  2:797070/81‑1 (MQ=255)                                                                         gCTGATCAGCTCATCTGTCAGCGCCGGAGACGTTGCCAGCGCCGCCGCTTCTGCCAGAACAGTAATGTTCTCCAGCAGACg   >  1:797147/1‑81 (MQ=255)                                                                          cTGATCAGCTCATCTGTCAGCGCCGGAGACGTTGCCAGCGCCGCCGCTTCTGCCAGAACAGTAATGTTCTCCAGCAGACGt  >  2:797144/1‑81 (MQ=255)                                                                              |                                                                             AAACCACTGTGCCTGAACATCGCGTTCGCGCAGGATCTCAACAAACAGCAGGGTCGACATCAGCTCGCCGTGGCTGACCAGCTCATCTGTCAGCGCCGGAGACGTTGCCAGCGCCGCCGCTTCTGCCAGAACAGTAATGTTCTCCAGCAGACGT  >  NC_000913/4232814‑4232967

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑