Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	399,509	T→C	intergenic (‑176/‑84)	yaiY ← / → yaiZ	inner membrane protein/DUF2754 domain‑containing protein YaiZ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	399,509	0	T	C	100.0%	95.1 / NA	28	intergenic (‑176/‑84)	yaiY/yaiZ	inner membrane protein/DUF2754 domain‑containing protein YaiZ
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (14/14);  total (14/14)

GTAAATTGATGTTGGGAATATTCCCGAAGCGTGAATCTTCGTTGTGAGTCACAAAATGGCAACCTTAAGTTAAATTTATTAACAATTACGAAAATGTTGTCTACACCACAGACGGTTGTTGCAGAATATGCAAGGATGTTGTTTTTCGTTAACG  >  NC_000913/399431‑399584                                                                               |                                                                            gTAAATTGATGTTGGGAATATTCCCGAAGCGTGAATCTTCGTTGTGAGTCACAAAATGGCAACATTAAGTTAAATttacta                                                                           <  1:81722/81‑1 (MQ=255)    aaTTGATGTTGGGAATATTCCCGAAGCGTGAATCTTCGTTGTGAGTCACAAAATGGCAACCTTAAGTTAAATTTACTAAc                                                                         <  1:81724/80‑1 (MQ=255)    aaTTGATGTTGGGAATATTCCCGAAGCGTGAATCTTCGTTGTGAGTCACAAAATGGCAACCTTAAGTTAAATTTACTAACa                                                                        <  2:81723/81‑1 (MQ=255)        gATGTTGGGAATATTCCCGAAGCGTGAATCTTCGTTGTGAGTCACAAAATGGCAACCTTAAGTTAAATTTACTAACAAtt                                                                     <  1:81725/80‑1 (MQ=255)         aTGTTGGGAATATTCCCGAAGCGTGAATCTTCGTTGTGAGTCACAAAATGGCAACCTTAAGTTAAATTTACTAACAATTa                                                                    <  2:81726/80‑1 (MQ=255)          tGTTGGGAATATTCCCGAAGCGTGAATCTTCGTTGTGAGTCACAAAATGGCAACCTTAAGTTAAATTTACTAACAATTACg                                                                  <  1:81727/81‑1 (MQ=255)               ggAATATTCCCGAAGCGTGAATCTTCGTTGTGAGTCACAAAATGGCAACCTTAAGTTAAATTTACTAACAATTACGaaaa                                                              <  2:81728/80‑1 (MQ=255)                     ttCCCGAAGCGTGAATCTTCGTTGTGAGTCACAAAATGGCAACCTTAAGTTAAATTTACTAACAATTACGAAAAtgttgt                                                        <  1:81730/80‑1 (MQ=255)                     ttCCCGAAGCGTGAATCTTCGTTGTGAGTCACAAAATGGCAACCTTAAGTTAAATTTACTAACAATTACGAAAATGTTGTc                                                       <  2:81729/81‑1 (MQ=255)                       cccGAAGCGTGAATCTTCGTTGTGAGTCACAAAATGGCAACCTTAAGTTAAATTTACTAACAATTACGAAAATGTTGTCTa                                                     >  2:81750/1‑81 (MQ=255)                          gAAGCGTGAATCTTCGTTGTGAGTCACAAAATGGCAACCTTAAGTTAAATTTACTAACAATTACGAAAATGTTGTCTacac                                                  >  2:81751/1‑81 (MQ=255)                                 gAATCTTCGTTGTGAGTCACAAAATGGCAACCTTAAGTTAAATTTACTAACAATTACGAAAATGTTGTCTACACCACAGAc                                           >  1:81761/1‑81 (MQ=255)                                        cGTTGTGAGTCACAAAATGGCAACCTTAAGTTAAATTTACTAACAATTACGAAAATGTTGTCTACACCACAGACGgttgtt                                    >  1:81752/1‑81 (MQ=255)                                             tgAGTCACAAAATGGCAACCTTAAGTTAAATTTACTAACAATTACGAAAATGTTGTCTACACCACAGACGGTTGTTGCAGa                               <  2:81731/81‑1 (MQ=255)                                                gTCACAAAATGGCAACCTTAAGTTAAATTTACTAACAATTACGAAAATGTTGTCTACACCACAGACGGTTGTTGCAGAata                            >  2:81759/1‑81 (MQ=255)                                                gTCACAAAATGGCAACCTTAAGTTAAATTTACTAACAATTACGAAAATGTTGTCTACACCACAGACGGTTGTTGCAGAata                            >  1:81756/1‑81 (MQ=255)                                                    caAAATGGCAACCTTAAGTTAAATTTACTAACAATTACGAAAAGGTGGTCTACACCACAGACGGTTGTTGCAGAATATGCa                        <  2:81732/81‑1 (MQ=255)                                                      aaaTGGCAACCTTAAGTTAAATTTACTAACAATTACGAAAATGTTGTCTACACCACAGACGGTTGTTGCAGAATATGCAAg                      >  2:81749/1‑81 (MQ=255)                                                       aaTGGCAACCTTAAGTTAAATTTACTAACAATTACGAAAATGTTGTCTACACCACAGACGGTTGTTGCAGAATATGCAAg                      >  1:81763/1‑80 (MQ=255)                                                             aaCCTTAAGTTAAATTTACTAACAATTACGAAAATGTTGTCTACACCACAGACGGTTGTTGCAGAATATGCAAGGAtgttg               >  1:81770/1‑81 (MQ=255)                                                               ccTTAAGTTAAATTTACTAACAATTACGAAAATGTTGTCTACACCACAGACGGTTGTTGCAGAATATGCAAGGATgttgtt             >  1:81781/1‑81 (MQ=255)                                                                  tAAGTTAAATTTACTAACAATTACGAAAATGTTGTCTACACCACAGACGGTTGTTGCAGAATATGCAAGGATGTTGttttt          >  1:81779/1‑81 (MQ=255)                                                                  tAAGTTAAATTTACTAACAATTACGAAAATGTTGTCTACACCACAGACGGCTGTTGCAGAATATGCAAGGATGTTGttttt          >  1:81760/1‑81 (MQ=255)                                                                   aaGTTAAATTTACTAACAATTACGAAAATGTTGTCTACACCACAGACGGTTGTTGCAGAATATGCAAGGATGTTGttttt          <  2:81733/80‑1 (MQ=255)                                                                     gTTAAATTTACTAACAATTACGAAAATGTTGTCTACACCACAGACGGTTGTTGCAGAATATGCAAGGATGTTGTTTTTCGt       >  1:81771/1‑81 (MQ=255)                                                                      ttAAATTTACTAACAATTACGAAAATGTTGTCTACACCACAGACGGTTGTTGCAGAATATGCAAGGATGTTGTTTTTCGtt      <  2:81734/81‑1 (MQ=255)                                                                        aaaTTTACTAACAATTACGAAAATGTTGTCTACACCACAGACGGTTGTTGCAGAATATGCAAGGATGTTGTTTTTCGTTaa    >  2:81783/1‑81 (MQ=255)                                                                        aaaTTTACTAACAATTACGAAAATGTTGTCTACACCACAGACGGTTGTTGCAGAATATGCAAGGATGTTGTTTTTCGTTa     <  2:81735/80‑1 (MQ=255)                                                                          aTTTACTAACAATTACGAAAATGTTGTCTACACCACAGACGGTTGTTGCAGAATATGCAAGGATGTTGTTTTTCGTTAACg  <  1:81736/81‑1 (MQ=255)                                                                               |                                                                            GTAAATTGATGTTGGGAATATTCCCGAAGCGTGAATCTTCGTTGTGAGTCACAAAATGGCAACCTTAAGTTAAATTTATTAACAATTACGAAAATGTTGTCTACACCACAGACGGTTGTTGCAGAATATGCAAGGATGTTGTTTTTCGTTAACG  >  NC_000913/399431‑399584

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑