Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,272,220	A→G	I551T (ATT→ACT)	uvrA ←	excision nuclease subunit A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,272,220	0	A	G	100.0%	178.9 / NA	51	I551T (ATT→ACT)	uvrA	excision nuclease subunit A
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (24/27);  total (24/27)

GCGGACCTTCTGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAGCGGCGCGAATTGCGTCTTCGTCGTGCTCCACCACAATCACGGTATTACCGAGATCGCGCAGATGGATAAGCGTACCCAACAGGCG  >  NC_000913/4272143‑4272296                                                                              |                                                                             gCGGACCTTCTGCGACCACTTCACCGCCGTGGACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAGCGGCGCGAGTTg                                                                           <  2:806748/81‑1 (MQ=255) gCGGACCTTCTGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAGCGGCGCGAGTTg                                                                           <  1:806750/81‑1 (MQ=255)   ggACCTTCTGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAGCGGCGCGAGTTGCg                                                                         <  2:806750/81‑1 (MQ=255)      ccTTCTGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAGCGGCGCGAGTTGCGTCt                                                                      <  1:806752/81‑1 (MQ=255)      ccTTCTGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAGCGGCGCGAGTTGCGTCt                                                                      <  2:806753/81‑1 (MQ=255)      ccTTCTGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAGCGGCGCGAGTTGCGTCt                                                                      <  2:806752/81‑1 (MQ=255)       cTTCTGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAGCGGCGCGAGTTGCGTCtt                                                                     <  1:806755/81‑1 (MQ=255)       cTTCTGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAGCGGCGCGAGTTGCGTCtt                                                                     >  1:806799/1‑81 (MQ=255)        ttCTGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAGCGGCGCGAGTTGCGTCTtc                                                                    >  2:806804/1‑81 (MQ=255)          cTGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAGCGGCGCGAGTTGCGTCTtc                                                                    <  1:806756/79‑1 (MQ=255)          cTGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAGCGGCGCGAGTTGCGTCTtc                                                                    <  1:806757/79‑1 (MQ=255)             cGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAGCGGCGCGAGTTGCGTCTtcgtcg                                                                <  1:806759/80‑1 (MQ=255)             cGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAGCGGCGCGAGTTGCGTCTtcgtc                                                                 <  1:806758/79‑1 (MQ=255)             cGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAGCGGCGCGAGTTGCGTCTTcgtcgt                                                               <  1:806760/81‑1 (MQ=255)              gACCACTTCACCGCCGTGAACTCCTGCGCCCGGGCCAATGCCGAGCACATGGTTAGCGGCGCGAGCTGCGCCCTCGCCGTg                                                              >  2:806814/1‑81 (MQ=255)              gACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAGCGGCGCGAGTTGCGTCTTCGTCGTg                                                              >  2:806812/1‑81 (MQ=255)                 cACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAGCGGCGCGAGTTGCGTCTTCGTCGTGCTc                                                           <  2:806760/81‑1 (MQ=255)                 cACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAGCGGCGCGAGTTGCGTCTTCGTCGTGCTc                                                           >  1:806821/1‑81 (MQ=255)                     tCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAGCGGCGCGAGTTGCGTCTTCGTCGTGCTccac                                                        >  2:806827/1‑80 (MQ=255)                      cACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAGCGGCGCGAGTTGCGTCTTCGTCGTGCTccacca                                                      >  1:806824/1‑81 (MQ=255)                      cACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAGCGGCGCGAGTTGCGTCTTCGTCGTGCTccacc                                                       <  2:806762/80‑1 (MQ=255)                      cACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAGCGGCGCGAGTTGCGTCTTCGTCGTGCTccacc                                                       <  1:806762/80‑1 (MQ=255)                       aCCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAGCGGCGCGAGTTGCGTCTTCGTCGTGCTccacca                                                      <  1:806764/80‑1 (MQ=255)                       aCCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAGCGGCGCGAGTTGCGTCTTCGTCGTGCTCcaccac                                                     <  2:806764/81‑1 (MQ=255)                          gccgTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAGCGGCGCGAGTTGCGTCTTCGTCGTGCTCCACCACAAt                                                  <  2:806765/81‑1 (MQ=255)                            cgTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAGCGGCGCGAGTTGCGTCTTCGTCGTGCTCCACCACAATCa                                                >  1:806803/1‑81 (MQ=255)                             gTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAGCGGCGCGAGTTGCGTCTTCGTCGTGCTCCACCACAATCAc                                               >  1:806822/1‑81 (MQ=255)                               gAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAGCGGCGCGAGTTGCGTCTTCGTCGTGCTCCACCACAATCACgg                                             >  1:806817/1‑81 (MQ=255)                                aaCACCTGCGCCCGGGCCAATGTCGATCACATGGTCAGCGGCGCGAGTTGCGTCTTCGTCGTGCTCCACCACAATCACgg                                             >  1:806808/1‑80 (MQ=255)                                 acacCTGCGCCCGGGCCAATGTCGATCACATGGTCAGCGGCGCGAGTTGCGTCTTCGTCGTGCTCCACCACAATCACGGTa                                           <  2:806766/81‑1 (MQ=255)                                  cacCTGCGCCCGGGCCAATGTCGATCACATGATCAGCGGCGCGAGTTGCGTCTTCGTCGTGCTCCACCACAATCACGGTAt                                          <  2:806767/81‑1 (MQ=255)                                   acCTGCGCCCGGGCCAATGTCGATCACATGGTCAGCGGCGCGAGTTGCGTCTTCGTCGTGCTCCACCACAATCACGGTAt                                          >  1:806811/1‑80 (MQ=255)                                    ccTGCGCCCGGGCCAATGTCGATCACATGGTCAGCGGCGCGAGTTGCGTCTTCGTCGTGCTCCACCACAATCACGGTATTa                                        >  2:806815/1‑81 (MQ=255)                                     cTGCGCCCGGGCCAATGTCGATCACATGGTCAGCGGCGCGAGTTGCGTCTTCGTCGTGCTCCACCACAATCACGGTATTAc                                       >  1:806839/1‑81 (MQ=255)                                          cccGGGCCAATGTCGATCACATGGTCAGCGGCGCGAGTTGCGTCTTCGTCGTGCTCCACCACAATCACGGTATTACCgaga                                  <  2:806768/81‑1 (MQ=255)                                           ccGGGCCAATGTCGATCACATGGTCAGCGGCGCGAGTTGCGTCTTCGTCGTGCTCCACCACAATCACGGTATTACCGAGAt                                 <  1:806770/81‑1 (MQ=255)                                              ggCCAATGTCGATCACATGGTCAGCGGCGCGAGTTGCGTCTTCGTCGTGCTCCACCACAATCACGGTATTACCGAGATcgc                              >  2:806809/1‑81 (MQ=255)                                               gCCAATGTCGATCACATGGTCAGCGGCGCGAGTTGCGTCTTCGTCGTGCTCCACCACAATCACGGTATTACCGAGATcgcg                             >  2:806822/1‑81 (MQ=255)                                               gCCAATGTCGATCACATGGTCAGCGGCGCGAGTTGCGTCTTCGTCGTGCTCCACCACAATCACGGTATTACCGAGATcgcg                             >  2:806829/1‑81 (MQ=255)                                                 cAATGTCGATCACATGGTCAGCGGCGCGAGTTGCGTCTTCGTCGTGCTCCACCACAATCACGGTATTACCGAGATcgcg                             >  1:806814/1‑79 (MQ=255)                                                  aaTGTCGATCACATGGTCAGCGGCGCGAGTTGCGTCTTCGTCGTGCTCCACCACAATCACGGTATTACCGAGATcgcg                             >  2:806835/1‑78 (MQ=255)                                                   aTGTCGATCACATGGTCAGCGGCGCGAGTTGCGTCTTCGTCGTGCTCCACCACAATCACGGTATTACCGAGATcgcg                             >  1:806861/1‑77 (MQ=255)                                                         aTCACATGGTCAGCGGCGCGAGTTGCGTCTTCGTCGTGCTCCACCACAATCACGGTATTACCGAGATCGCGCAGATGGAt                    >  1:806820/1‑80 (MQ=255)                                                            acaTGGTCAGCGGCGCGAGTTGCGTCTTCGTCGTGCTCCACCACAATCACGGTATTACCGAGATCGCGCAGATGGATAAg                 <  2:806770/80‑1 (MQ=255)                                                            acaTGGTCAGCGGCGCGAGTTGCGTCTTCGTCGTGCTCCACCACAATCACGGTATTACCGAGATCGCGCAGATGGATAAGc                >  1:806826/1‑81 (MQ=255)                                                             caTGGTCAGCGGCGCGAGTTGCGTCTTCGTCGTGCTCCACCACAATCACGGTATTACCGAGATCGCGCAGATGGATAAGCg               <  1:806772/81‑1 (MQ=255)                                                              aTGGTCAGCGGCGCGAGTTGCGTCTTCGTCGTGCTCCACCACAATCACGGTATTACCGAGATCGCGCAGATGGATAAGCg               >  1:806819/1‑80 (MQ=255)                                                                 gTCAGCGGCGCGAGTTGCGTCTTCGTCGTGCTCCACCACAATCACGGTATTACCGAGATCGCGCAGATGGATAAGCGTAcc           <  1:806773/81‑1 (MQ=255)                                                                  tCAGCGGCGCGAGTTGCGTCTTCGTCGTGCTCCACCACAATCACGGTATTACCGAGATCGCGCAGATGGATAAGCGTAccc          <  2:806773/81‑1 (MQ=255)                                                                    aGCGGCGCGAGTTGCGTCTTCGTCGTGCTCCACCACAATCACGGTATTACCGAGATCGCGCAGATGGATAAGCGTACCCa         <  2:806774/80‑1 (MQ=255)                                                                          gcgAGTTGCGTCTTCGTCGTGCTCCACCACAATCACGGTATTACCGAGATCGCGCAGATGGATAAGCGTACCCAACAGgcg  >  1:806818/1‑81 (MQ=255)                                                                              |                                                                             GCGGACCTTCTGCGACCACTTCACCGCCGTGAACACCTGCGCCCGGGCCAATGTCGATCACATGGTCAGCGGCGCGAATTGCGTCTTCGTCGTGCTCCACCACAATCACGGTATTACCGAGATCGCGCAGATGGATAAGCGTACCCAACAGGCG  >  NC_000913/4272143‑4272296

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑