Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,272,422	C→T	A484T (GCA→ACA)	uvrA ←	excision nuclease subunit A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,272,422	0	C	T	100.0%	117.4 / NA	33	A484T (GCA→ACA)	uvrA	excision nuclease subunit A
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (12/21);  total (12/21)

ATAACGCCAACCAGGCCCGCACCAATCTGGCTCGCCAGACGGATACGCTGTGCTTCACCGCCAGAAAGCGTTTCTGCCGAGCGGGAAAGCGTCAGGTAATTCAGGCCGACGTTAACGAGGAATTTCAGACGATCGCCGATCTCTTTAAGG  >  NC_000913/4272346‑4272495                                                                             |                                                                          aTAACGCCAACCAGGCCCGCACCAATCTGGCTCGCCAGACGGATACGCTGTGCTTCACCGCCAGAAAGCGTTTCTGTCGAg                                                                       <  2:806805/81‑1 (MQ=255)  tAACGCCAACCAGGCCCGCACCAATCTGGCTCGCCAGACGGATACGCTGTGCTTCACCGGCAGAAAGCGTTTCTGTCGAGc                                                                      <  2:806807/81‑1 (MQ=255)  tAACGCCAACCAGGCCCGCACCAATCTGGCTCGCCAGACGGATACGCTGTGCTTCACCGCCAGAAAGCGTTTCTGTCGAGc                                                                      <  1:806807/81‑1 (MQ=255)   aaCGCCAACCAGGCCCGCACCAATCTGGCTCGCCAGACGGATACGCTGTGCTTCACCGCCAGAAAGCGTTTCTGTCGAGCg                                                                     >  2:806863/1‑81 (MQ=255)    aCGCCAACCAGGCCCGCACCAATCTGGCTCGCCAGACGGATACGCTGTGCTTCACCGCCAGAAAGCGTTTCTGTCGAGCgg                                                                    >  2:806861/1‑81 (MQ=255)    aCGCCAACCAGGCCCGCACCAATCTGGCTCGCCAGACGGATACGCTGTGCTTCACCGCCAGAAAGCGTTTCTGTCGAGCgg                                                                    <  1:806809/81‑1 (MQ=255)    aCGCCAACCAGGCCCGCACCAATCTGGCTCGCCAGACGGATACGCTGTGCTTCACCGCCAGAAAGCGTTTCTGTCGAGCg                                                                     <  1:806810/80‑1 (MQ=255)       ccAACCAGGCCCGCACCAATCTGGCTCGCCAGACGGATACGCTGTGCTTCACCGCCAGAAAGCGTTTCTGTCGAGCGGGaa                                                                 <  2:806810/81‑1 (MQ=255)         aaCCAGGCCCGCACCAATCTGGCTCGCCAGACGGATACGCTGTGCTTCACCGCCAGAAAGCGTTTCTGTCGAGCGGGAAAg                                                               <  1:806812/81‑1 (MQ=255)          aCCAGGCCCGCACCAATCTGGCTCGCCAGACGGATACGCTGTGCTTCACCGCCAGAAAGCGTTTCTGTCGAGCGGGAAAGc                                                              <  1:806813/81‑1 (MQ=255)                cccGCACCAATCTGGCTCGCCAGACGGATACGCTGTGCTTCACCGCCAGAAAGCGTTTCTGTCGAGCGGGAAAGCGTCAgg                                                        <  2:806813/81‑1 (MQ=255)                     aCCAATCTGGCTCGCCAGACGGATACGCTGTGCTTCACCGCCAGAAAGCGTTTCTGTCGAGCGGGAAAGCGTCAGGTAAtt                                                   >  2:806857/1‑81 (MQ=255)                     aCCAATCTGGCTCGCCAGACGGATACGCTGTGCTTCACCGCCAGAAAGCGTTTCTGTCGAGCGGGAAAGCGTCAGGTAAtt                                                   >  1:806876/1‑81 (MQ=255)                              gCTCGCCAGACGGATACGCTGTGCTTCACCGCCAGAAAGCGTTTCTGTCGAGCGGGAAAGCGTCAGGTAATTCAGGcc                                             <  2:806816/78‑1 (MQ=255)                              gCTCGCCAGACGGATACGCTGTGCTTCACCGCCAGAAAGCGTTTCTGTCGAGCGGGAAAGCGTCAGGTAATTCAGGCCGAc                                          <  1:806816/81‑1 (MQ=255)                              acTCGCCAGACGGATACGCTGTGCTTCACCGCCAGAAAGCGTTTCTGTCGAGCGGGAAAGCGTCAGGTAATTCAGGCCGAc                                          <  1:806815/80‑1 (MQ=255)                               cTCGCCAGACGGATACGCTGTGCTTCACCGCCAGAAAGCGTTTCTGTCGAGCGGGAAAGCGTCAGGTAATTCAGGCCGACg                                         <  2:806817/81‑1 (MQ=255)                                 cGCCAGACGGATACGCTGTGCTTCACCGCCAGAAAGCGTTTCTGTCGAGCGGGAAAGCGTCAGGTAATTCAGGCCGACGt                                        >  2:806867/1‑80 (MQ=255)                                  gCCAGACGGATACGCTGTGCTTCACCGCCAGAAAGCGTTTCTGTCGAGCGGGAAAGCGTCAGGTAATTCAGGCCGACGtt                                       <  2:806818/80‑1 (MQ=255)                                  gCCAGACGGATACGCTGTGCTTCACCGCCAGAAAGCGTTTCTGTCGAGCGGGAAAGCGTCAGGTAATTCAGGCCGACGtt                                       >  2:806869/1‑80 (MQ=255)                                    cAGACGGATACGCTGTGCTTCACCGCCAGAAAGCGTTTCTGTCGAGCGGGAAAGCGTCAGGTAATTCAGGCCGACGTTAAc                                    <  2:806819/81‑1 (MQ=255)                                     aGACGGATACGCTGTGCTTCACCGCCAGAAAGCGTTTCTGTCGAGCGGGAAAGCGTCAGGTAATTCAGGCCGACGTTAAc                                    >  1:806872/1‑80 (MQ=255)                                       aCGGATACGCTGTGCTTCACCGCCAGAAAGCGTTTCTGTCGAGCGGGAAAGCGTCAGGTAATTCAGGCCGACGTTAACGAg                                 >  2:806866/1‑81 (MQ=255)                                        cGGATACGCTGTGCTTCACCGCCAGAAAGCGTTTCTGTCGAGCGGGAAAGCGTCAGGTAATTCAGGCCGACGTTAACGAgg                                <  2:806820/81‑1 (MQ=255)                                                cTGTGCTTCACCGCCAGAAAGCGTTTCTGTCGAGCGGGAAAGCGTCAGGTAATTCAGGCCGACGTTAACGAGGAATTTCAg                        <  2:806821/81‑1 (MQ=255)                                                   tgCTTCACCGCCAGAAAGCGTTTCTGTCGAGCGGGAAAGCGTCAGGTAATTCAGGCCGACGTTAACGAGGAATTTCAGa                       <  1:806823/79‑1 (MQ=255)                                                       tCACCGCCAGAAAGCGTTTCTGTCGAGCGGGAAAGCGTCAGGTAATTCAGGCCGACGTTAACGAGGAATTTCAGACGATCg                 >  2:806862/1‑81 (MQ=255)                                                         aCCGCCAGAAAGCGTTTCTGTCGAGCGGGAAAGCGTCAGGTAATTCAGGCCGACGTTAACGAGGAATTTCAGACGATCGcc               >  2:806873/1‑81 (MQ=255)                                                         aCCGCCAGAAAGCGTTTCTGTCGAGCGGGAAAGCGTCAGGTAATTCAGGCCGACGTTAACGAGGAATTTCAGACGATCGc                <  2:806823/80‑1 (MQ=255)                                                               aGAAAGCGTTTCTGTCGAGCGGGAAAGCGTCAGGTAATTCAGGCCGACGTTAACGAGGAATTTCAGACGATCGCCGAtctc         >  1:806866/1‑81 (MQ=255)                                                                 aaaGCGTTTCTGTCGAGCGGGAAAGCGTCAGGTAATTCAGGCCGACGTTAACGAGGAATTTCAGACGATCGCCGATCTCtt       <  2:806824/81‑1 (MQ=255)                                                                      gTTTCTGTCGAGCGGGAAAGCGTCAGGTAATTCAGGCCGACGTTAACGAGGAATTTCAGACGATCGCCGATCTCTTTAAgg  <  2:806825/81‑1 (MQ=255)                                                                      gTTTCTGTCGAGCGGGAAAGCGTCAGGTAATTCAGGCCGACGTTAACGAGGAATTTCAGACGATCGCCGATCTCTTTAAg   >  2:806884/1‑80 (MQ=255)                                                                             |                                                                          ATAACGCCAACCAGGCCCGCACCAATCTGGCTCGCCAGACGGATACGCTGTGCTTCACCGCCAGAAAGCGTTTCTGCCGAGCGGGAAAGCGTCAGGTAATTCAGGCCGACGTTAACGAGGAATTTCAGACGATCGCCGATCTCTTTAAGG  >  NC_000913/4272346‑4272495

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑