Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,288,159	T→C	R132R (CGT→CGC)	nrfA →	cytochrome c552 nitrite reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,288,159	0	T	C	100.0%	154.7 / NA	45	R132R (CGT→CGC)	nrfA	cytochrome c552 nitrite reductase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (25/20);  total (25/20)

ACCGGTGCGCCGAAAAACGCTGAAGATGGTCCGCTACCGATGGCATGCTGGAGTTGTAAAAGCCCGGATGTGGCGCGTCTGATCCAGAAAGACGGCGAAGATGGCTACTTCCACGGTAAATGGGCGCGCGGCGGTCCGGAAATCGTCAAC  >  NC_000913/4288082‑4288231                                                                              |                                                                         aCCGGTGCGCCGAAAAACGCTGAAGATGGTCCGCTACCGATGGCATGCTGGAGTTGTAAAAGCCCGGATGTGGCGCGCCt                                                                        >  2:810662/1‑80 (MQ=255)   cGGTGCGCCGAAAAACGCTGAAGATGGTCCGCTACCGATGGCATGCTGGAGTTGTAAAAGCCCGGATGTGGCGCGCCTGAt                                                                     >  2:810652/1‑81 (MQ=255)    ggTGCGCCGAAAAACGCTGAAGATGGTCCGCTACCGATGGCATGCTGGAGTTGTAAAAGCCCGGATGTGGCGCGCCTGATc                                                                    >  2:810668/1‑81 (MQ=255)        cgcCGAAAAACGCTGAAGATGGTCCGCTACCGATGGCATGCTGGAGTTGTAAAAGCCCGGATGTGGCGCGCCTGATCCAg                                                                 >  1:810645/1‑80 (MQ=255)         gcCGAAAAACGCTGAAGATGGTCCGCTACCGATGGCATGCTGGAGTTGTAAAAGCCCGGATGTGGCGCGCCTGATCCAGaa                                                               <  2:810602/81‑1 (MQ=255)            gAAAAACGCTGAAGATGGTCCGCTACCGATGGCATGCTGGAGTTGTAAAAGCCCGGATGTGGCGCGCCTGATCCAGAAAGa                                                            <  1:810604/81‑1 (MQ=255)               aaaCGCTGAAGATGGTCCGCTACCGATGGCATGCTGGAGTTGTAAAAGCCCGGATGTGGCGCGCCTGATCCAGAAAGACgg                                                         >  2:810647/1‑81 (MQ=255)               aaaCGCTGAAGATGGTCCGCTACCGATGGCATGCTGGAGTTGTAAAAGCCCGGATGTGGCGCGCCTGATCCAGAAAGACgg                                                         >  2:810651/1‑81 (MQ=255)                 aCGCTGAAGATGGTCCGCTACCGATGGCATGCTGGAGTTGTAAAAGCCCGGATGTGGCGCGCCTGATCCAGAAAGACGGc                                                        >  1:810656/1‑80 (MQ=255)                 aCGCTGAAGATGGTCCGCTACCGATGGCATGCTGGAGTTGTAAAAGCCCGGATGTGGCGCGCCTGATCCAGAAAGACGGCg                                                       <  2:810604/81‑1 (MQ=255)                  cGCTGAAGATGGTCCGCTACCGATGGCATGCTGGAGTTGTAAAAGCCCGGATGTGGCGCGCCTGATCCAGAAAGACGGCGa                                                      <  1:810606/81‑1 (MQ=255)                  cGCTGAAGATGGTCCGCTACCGATGGCATGCTGGAGTTGTAAAAGCCCGGAGGTGGCGCGCCTGATCCAGAAAGACGGCg                                                       >  1:810650/1‑80 (MQ=255)                   gCTGAAGATGGTCCGCTACCGATGGCATGCTAGAGTTGTAAAAGCCCGGATGTGGCGCGCCTGATCCAGAAAGACGGCGaa                                                     <  2:810606/81‑1 (MQ=255)                       aaGATGGTCCGCTACCGATGGCATGCTGGAGTTGTAAAAGCCCGGATGTGGCGCGCCTGATCCAGAAAGACGGCGAAGa                                                   <  1:810608/79‑1 (MQ=255)                             gTCCGCTACCGATGGCATGCTGGAGTTGTAAAAGCCCGGATGTGGCGCGCCTGATCCAGAAAGACGGCGAAGATGGCTACt                                           <  2:810608/81‑1 (MQ=255)                                  cTACCGATGGCATGCTGGAGTTGTAAAAGCCCGGATGTGGCGCGCCTGATCCAGAAAGACGGCGAAGATGGCTACTTCCAc                                      >  2:810664/1‑81 (MQ=255)                                  cTACCGATGGCATGCTGGAGTTGTAAAAGCCCGGATGTGGCGCGCCTGATCCAGAAAGACGGCGAAGATGGCTACTTCCAc                                      <  1:810610/81‑1 (MQ=255)                                   tACCGATGGCATGCTGGAGTTGTAAAAGCCCGGATGTGGCGCGCCTGATCCAGAAAGACGGCGAAGATGGCTACTTCCACg                                     >  2:810653/1‑81 (MQ=255)                                     ccGATGGCATGCTGGAGTTGTAAAAGCCCGGATGTGGCGCGCCTGATCCAGAAAGACGGCGAAGATGGCTACTTCCACgg                                    >  1:810667/1‑80 (MQ=255)                                     ccGATGGCATGCTGGAGTTGTAAAAGCCCGGATGTGGCGCGCCTGATCCAGAAAGACGGCGAAGATGGCTACTTCCACGGt                                   <  2:810610/81‑1 (MQ=255)                                      cGATGGCATGCTGGAGTTGTAAAAGCCCGGATGTGGCGCGCCTGATCCAGAAAGACGGCGAAGATGGCTACTTCCACGGt                                   >  2:810679/1‑80 (MQ=255)                                       gATGGCATGCTGGAGTTGTAAAAGCCCGGATGTGGCGCGCCTGATCCAGAAAGACGGCGAAGATGGCTACTTCCACGGTa                                  >  1:810659/1‑80 (MQ=255)                                       gATGGCATGCTGGAGTTGTAAAAGCCCGGATGTGGCGCGCCTGATCCAGAAAGACGGCGAAGATGGCTACTTCCACGGTa                                  >  2:810660/1‑80 (MQ=255)                                         tGGCATGCTGGAGTTGTAAAAGCCCGGATGTGGCGCGCCTGATCCAGAAAGACGGCGAAGATGGCTACTTCCACGGTAAAt                               <  1:810612/81‑1 (MQ=255)                                          ggCATGCTGGAGTTGTAAAAGCCCGGATGTGGCGCGCCTGATCCAGAAAGACGGCGAAGATGGCTACTTCCACGGTAAATg                              >  2:810669/1‑81 (MQ=255)                                             aTGCTGGAGTTGTAAAAGCCCGGATGTGGCGCGCCTGATCCAGAAAGACGGCGAAGATGGCTACTTCCACGGTAAATGGgc                           >  2:810667/1‑81 (MQ=255)                                              tGCTGGAGTTGTAAAAGCCCGGATGTGGCGCGCCTGATCCAGAAAGACGGCGAAGATGGCTACTTCCACGGTAAATGGgcg                          <  2:810612/81‑1 (MQ=255)                                                cTGGAGTTGTAAAAGCCCGGATGTGGCGCGCCTGATCCAGAAAGACGGCGAAGATGGCTACTTCCACGGTAAATGGgcgc                         <  2:810614/80‑1 (MQ=255)                                                cTGGAGTTGTAAAAGCCCGGATGTGGCGCGCCTGATCCAGAAAGACGGCGAAGATGGCTACTTCCACGGTAAATGGgcgc                         <  2:810613/80‑1 (MQ=255)                                                  ggAGTTGTAAAAGCCCGGATGTGGCGCGCCTGATCCAGAAAGACGGCGAAGATGGCTACTTCCACGGTAAATGGGcgcgcg                      >  2:810656/1‑81 (MQ=255)                                                  ggAGTTGTAAAAGCCCGGATGTGGCGCGCCTGATCCAGAAAGACGGCGAAGATGGCTACTTCCACGGTAAATGGGcgcgcg                      >  1:810675/1‑81 (MQ=255)                                                      ttGTAAAAGCCCGGATGTGGCGCGCCTGATCCAGAAAGACGGCGAAGATGGCTACTTCCACGGTAAATGGGCGCGcggcgg                  <  1:810616/81‑1 (MQ=255)                                                      ttGTAAAAGCCCGGATGTGGCGCGCCTGATCCAGAAAGACGGCGAAGATGGCTACTTCCACGGTAAATGGGCGCGcggcgg                  >  1:810662/1‑81 (MQ=255)                                                         tAAAAGCCCGGATGTGGCGCGCCTGATCCAGAAAGACGGCGAAGATGGCTACTTCCACGGTAAATGGGCGCGCGGCGGTc                <  1:810617/80‑1 (MQ=255)                                                           aaaGCCCGGATGTGGCGCGCCTGATCCAGAAAGACGGCGAAGATGGCTACTTCCACGGTAAATGGGCGCGCGGCGGTCCgg             <  2:810617/81‑1 (MQ=255)                                                              gCCCGGATGTGGCGCGCCTGATCCAGAAAGACGGCGAAGATGGCTACTTCCACGGTAAATGGGCGCGCGGCGGTCCGGaaa          >  2:810659/1‑81 (MQ=255)                                                                ccGGATGTGGCGCGCCTGATCCAGAAAGACGGCGAAGATGGCTACTTCCACGGTAAATGGGCGCGCGGCGGTCCGGAAATc        <  2:810618/81‑1 (MQ=255)                                                                  ggATGTGGCGCGCCTGATCCAGAAAGACGGCGAAGATGGCTACTTCCACGGTAAATGGGCGCGCGGCGGTCCGGAAATCGt      <  2:810619/81‑1 (MQ=255)                                                                   gATGTGGCGCGCCTGATCCAGAAAGACGGCGAAGATGGCTACTTCCACGGTAAATGGGCGCGCGGCGGTCCGGAAATCGt      >  2:810681/1‑80 (MQ=255)                                                                   gATGTGGCGCGCCTGATCCAGAAAGACGGCGAAGATGGCTACTTCCACGGTAAATGGGCGCGCGGCGGTCCGGAAATCGt      >  2:810678/1‑80 (MQ=255)                                                                   gATGTGGCGCGCCTGATCCAGAAAGACGGCGAAGATGGCTACTTCCACGGTAAATGGGCGCGCGGCGGTCCGGAAATCGTc     <  2:810620/81‑1 (MQ=255)                                                                      gtgGCGCGCCTGATCCAGAAAGACGGCGAAGATGGCTACTTCCACGGTAAATGGGCGCGCGGCGGTCCGGAAATCGTcaa   >  1:810664/1‑80 (MQ=255)                                                                      gtgGCGCGCCTGATCCAGAAAGACGGCGAAGATGGCTACTTCCACGGTAAATGGGCGCGCGGCGGTCCGGAAATCGTcaa   >  1:810678/1‑80 (MQ=255)                                                                      gtgGCGCGCCTGATCCAGAAAGACGGCGAAGATGGCTACTTCCACGGTAAATGGGCGCGCGGCGGTCCGGAAATCGTcaa   >  1:810686/1‑80 (MQ=255)                                                                       tgGCGCGCCTGATCCAGAAAGACGGCGAAGATGGCTACTTCCACGGTAAATGGGCGCGCGGCGGTCCGGAAATCGTcaac  <  2:810621/80‑1 (MQ=255)                                                                              |                                                                         ACCGGTGCGCCGAAAAACGCTGAAGATGGTCCGCTACCGATGGCATGCTGGAGTTGTAAAAGCCCGGATGTGGCGCGTCTGATCCAGAAAGACGGCGAAGATGGCTACTTCCACGGTAAATGGGCGCGCGGCGGTCCGGAAATCGTCAAC  >  NC_000913/4288082‑4288231

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑