Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,297,871	T→C	Q499R (CAG→CGG)	fdhF ←	formate dehydrogenase H

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,297,871	0	T	C	100.0%	137.0 / NA	39	Q499R (CAG→CGG)	fdhF	formate dehydrogenase H
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (20/19);  total (20/19)

AAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCGGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGGTGGCGATTTCACTGATGATTTGCCAGTCCGTTTTCAGATCC  >  NC_000913/4297793‑4297948                                                                               |                                                                              aaGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCGGGCACAGATGACGCAACTCATCCCAGATCTCCCgg                                                                             >  1:813204/1‑81 (MQ=255)        gTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCGGGCACAGATGACGCAACTCATCCCAGATCTCCCGGGtgttgt                                                                      <  1:813161/81‑1 (MQ=255)          tCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCGGGCACAGATGACGCAACTCATCCCAGATCTCCCGGGtgttgttg                                                                    >  2:813224/1‑81 (MQ=255)            gCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCGGGCACAGATGACGCAACTCATCCCAGATCTCCCGGGtgttgttg                                                                    >  1:813208/1‑79 (MQ=255)            gCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCGGGCACAGATGACGCAACTCATCCCAGATCTCCCGGGTGTTGTTGTa                                                                  <  1:813162/81‑1 (MQ=255)              ccATTTTCTCGTAAGTCGCACCGTAGAAATCCGGGCACAGATGACGCAACTCATCCCAGATCTCCCGGGTGTTGTTGTAGt                                                                >  1:813213/1‑81 (MQ=255)               cATTTTCTCGTAAGTCGCACCGTAGAAATCCGGGCACAGATGACGCAACTCATCCCAGATCTCCCGGGTGTTGTTGTAGTg                                                               >  1:813209/1‑81 (MQ=255)                aTTTTCTCGTAAGTCGCACCGTAGAAATCCGGGCACAGATGACGCAACTCATCCCAGATCTCCCGGGTGTTGTTGTAGTg                                                               >  1:813218/1‑80 (MQ=255)                aTTTTCTCGTAAGTCGCACCGTAGAAATCCGGGCACAGATGACGCAACTCATCCCAGATCTCCCGGGTGTTGTTGTAGTGc                                                              <  1:813163/81‑1 (MQ=255)                    tctcGTAAGTCGCACCGTAGAAATCCGGGCACAGATGACGCAACTCATCCCAGATCTCCCGGGTGTTGTTGTAGTGCATCg                                                          >  2:813199/1‑81 (MQ=255)                       cGTAAGTCGCACCGTAGAAATCCGGGCACAGATGACGCAACTCATCCCAGATCTCCCGGGTGTTGTTGTAGTGCATCGGa                                                        >  2:813210/1‑80 (MQ=255)                          aaGTCGCACCGTAGAAATCCGGGCACAGATGACGCAACTCATCCCAGATCTCCCGGGTGTTGTTGTAGTGCATCGGATAAc                                                    >  1:813214/1‑81 (MQ=255)                                   cGTAGAAATCCGGGCACAGATGACGCAACTCATCCCAGATCTCCCGGGTGTTGTTGTAGTGCATCGGATAACCCATACggg                                           >  1:813215/1‑81 (MQ=255)                                    gTAGAAATCCGGGCACAGATGACGCAACTCATCCCAGATCTCCCGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGGt                                          >  1:813207/1‑81 (MQ=255)                                    gTAGAAATCCGGGCACAGATGACGCAACTCATCCCAGATCTCCCGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGGt                                          <  2:813163/81‑1 (MQ=255)                                     tAGAAATCCGGGCACAGATGACGCAACTCATCCCAGATCTCCCGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGGTg                                         <  2:813164/81‑1 (MQ=255)                                     tAGAAATCCGGGCACAGATGACGCAACTCATCCCAGATCTCCCGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGGTg                                         <  2:813165/81‑1 (MQ=255)                                       gAAATCCGGGCACAGATGACGCAACTCATCCCAGATCTCCCGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGGTGGc                                       <  2:813166/81‑1 (MQ=255)                                       gAAATCCGGGCACAGATGACGCAACTCATCCCAGATCTCCCGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGGTGGc                                       <  2:813167/81‑1 (MQ=255)                                        aaaTCCGGGCACAGATGACGCAACTCATCCCAGATCTCCCGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGGTGGc                                       >  1:813228/1‑80 (MQ=255)                                        aaaTCCGGGCACAGATGACGCAACTCATCCCAGATCTCCCGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGGTGGCg                                      <  1:813169/81‑1 (MQ=255)                                        aaaTCCGGGCACAGATGACGCAACTCATCCCAGATCTCCCGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGGTGGCg                                      <  2:813169/81‑1 (MQ=255)                                          aTCCGGGCACAGATGACGCAACTCATCCCAGATCTCCCGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGGTGGCGAt                                    <  2:813170/81‑1 (MQ=255)                                           tCCGGGCACAGATGACGCAACTCATCCCAGATCTCCCGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGGTGGCGAtt                                   <  2:813171/81‑1 (MQ=255)                                             cGGGCACAGATGACGCAACTCATCCCAGATCTCCCGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGGTGGCGATTTc                                 <  2:813172/81‑1 (MQ=255)                                              gggCACAGATGACGCAACTCATCCCAGATCTCCCGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGGTGGCGATTTCa                                >  1:813226/1‑81 (MQ=255)                                               ggCACAGATGACGCAACTCATCCCAGATCTCCCGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGGTGGCGATTTCAc                               >  2:813216/1‑81 (MQ=255)                                                 cacaGATGACGCAACTCATCCCAGATCTCCCGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGGTGGCGATTTCACtg                             <  2:813173/81‑1 (MQ=255)                                                   caGATGACGCAACTCATCCCAGATCTCCCGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGGTGGCGATTTCACtgat                           >  2:813228/1‑81 (MQ=255)                                                    aGATGACGCAACTCATCCCAGATCTCCCGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGGTGGCGATTTCACtgatg                          <  2:813174/81‑1 (MQ=255)                                                    aGATGACGCAACTCATCCCAGATCTCCCGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGGTGGCGATTTCACtgat                           >  1:813212/1‑80 (MQ=255)                                                           gctaCTCATCCCAGATCTCCCGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGGTGGCGATTTCACTGATGATTTGcc                   <  2:813175/78‑1 (MQ=255)                                                               cTCATCCCAGATCTCCCGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGGTGGCGATTTCACTGATGATTTGCCAGTc               <  1:813177/81‑1 (MQ=255)                                                                  aTCCCAGATCTCCCGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGGTGGCGATTTCACTGATGATTTGCCAGTCCGt            >  1:813223/1‑81 (MQ=255)                                                                   tCCCAGATCTCCCGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGGTGGCGATTTCACTGATGATTTGCCAGTCCGtt           <  1:813178/81‑1 (MQ=255)                                                                     ccAGATCTCCCGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGGTGGCGATTTCACTGATGATTTGCCAGTCCGtttt         >  1:813230/1‑81 (MQ=255)                                                                       aGATCTCCCGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGGTGGCGATTTCACTGATGATTTGCCAGTCCGtttt         >  1:813236/1‑79 (MQ=255)                                                                       aGATCTCCCGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGGTGGCGATTTCACTGATGATTTGCCAGTCCGTTTTCa       <  1:813179/81‑1 (MQ=255)                                                                            tcCCGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGGTGGCGATTTCACTGATGATTTGCCAGTCCGTTTTCAGATcc  >  2:813223/1‑81 (MQ=255)                                                                               |                                                                              AAGCCCAGTTCGCCCATTTTCTCGTAAGTCGCACCGTAGAAATCCGGGCACAGATGACGCAACTCATCCCAGATCTCCTGGGTGTTGTTGTAGTGCATCGGATAACCCATACGGGTGGCGATTTCACTGATGATTTGCCAGTCCGTTTTCAGATCC  >  NC_000913/4297793‑4297948

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑