Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	404,920	A→G	V242A (GTA→GCA)	proC ←	pyrroline‑5‑carboxylate reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	404,920	0	A	G	100.0%	83.1 / NA	25	V242A (GTA→GCA)	proC	pyrroline‑5‑carboxylate reductase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (13/12);  total (13/12)

GCTGAGTTTTTCTGATTTTTCCATACACTTCGTCATCGCTTCGATCACTGCAGCACGGAAGCCTTTCTCTTCCAGTACGCGTACCGCTTCAATGGTGGTGCCTCCCGGTGAGCAGACCATATCTTTCAGTGCCCCCGGATGTTCTCCCGTTTC  >  NC_000913/404844‑404996                                                                             |                                                                             gCTGAGTTTTTCTGATTTTTCCATACACTTCGTCATCGCTTCGATCACTGCAGCACGGAAGCCTTTCTCTTCCAGTgcgc                                                                           <  2:82654/80‑1 (MQ=255)    gAGTTTTTCTGATTTTTCCATACACTTCGTCATCGCTTCGATCACTGCAGCACGGAAGCCTTTCTCTTCCAGTGCGCGTAc                                                                       >  1:82692/1‑81 (MQ=255)          ttCTGATTTTTCCATACACTTCGTCATCGCTTCGATCACTGCAGCACGGAAGCCTTTCTCTTCCAGTGCGCGTACCGCtt                                                                  <  1:82655/80‑1 (MQ=255)              gATTTTTCCATACACTTCGTCATCGCTTCGATCACTGCAGCACGGAAGCCTTTCTCTTCCAGTGCGCGTACCGCTTCAAtg                                                             >  2:82702/1‑81 (MQ=255)               aTTTTTCCATACACTTCGTCATCGCTTCGATCACTGCAGCACGGAAGCCTTTCTCTTCCAGTGCGCGTACCGCTTCAAt                                                              <  1:82656/79‑1 (MQ=255)                  tttCCATACACTTCGTCATCGCTTCGATCACTGCAGCACGGAAGCCTTTCTCTTCCAGTGCGCGTACCGCTTCAAtggtgg                                                         >  2:82685/1‑81 (MQ=255)                   ttCCATACACTTCGTCATCGCTTCGATCACTGCAGCACGGAAGCCTTTCTCTTCCAGTGCGCGTACCGCTTCAATggtggt                                                        >  2:82704/1‑81 (MQ=255)                    tCCATACACTTCGTCATCGCTTCGATCACTGCAGCACGGAAGCCTTTCTCTTCCAGTGCGCGTACCGCTTCAATggtggt                                                        <  1:82657/80‑1 (MQ=255)                     ccATACACTTCGTCATCGCTTCGATCACTGCAGCACGGAAGCCTTTCTCTTCCAGTGCGCGTACCGCTTCAATGGTGGTGc                                                      >  2:82703/1‑81 (MQ=255)                       aTACACTTCGTCATCGCTTCGATCACTGCAGCACGGAAGCCTTTCTCTTCCAGTGCGCGTACCGCTTCAATGGTGGTGCCt                                                    >  1:82686/1‑81 (MQ=255)                         acacTTCGTCATCGCTTCGATCACTGCAGCACGGAAGCCTTTCTCTTCCAGTGCGCGTACCGCTTCAATGGTGGTGCCTcc                                                  >  1:82695/1‑81 (MQ=255)                              tCGTCATCGCTTCGATCACTGCAGCACGGAAGCCTTTCTCTTCCAGTGCGCGTACCGCTTCAATGGTGGTGCCTCCCGGt                                              >  1:82698/1‑80 (MQ=255)                                 tCATCGCTTCGATCACTGCAGCACGGAAGCCTTTCTCTTCCAGTGCGCGTACCGCTTCAATGGTGGTGCCTCCCGGTGAGc                                          <  1:82658/81‑1 (MQ=255)                                      gCTTCGATCACTGCAGCACGGAAGCCTTTCTCTTCCAGTGCGCGTACCGCTTCAATGGTGGTGCCTCCCGGTGAGCAGAcc                                     <  1:82659/81‑1 (MQ=255)                                       cTTCGATCACTGCAGCACGGAAGCCTTTCTCTTCCAGTGCGCGTACCGCTTCAATGGTGGTGCCTCCCGGTGAGCAGACCa                                    >  1:82688/1‑81 (MQ=255)                                           gATCACTGCAGCACGGAAGCCTTTCTCTTCCAGTGCGCGTACCGCTTCAATGGTGGTGCCTCCCGGTGAGCAGACCatat                                 <  1:82661/80‑1 (MQ=255)                                           gATCACTGCAGCACGGAAGCCTTTCTCTTCCAGTGCGCGTACCGCTTCAATGGTGGTGCCTCCCGGTGAGCAGACCATATc                                <  1:82660/81‑1 (MQ=255)                                             tCACTGCAGCACGGAAGCCTTTCTCTTCCAGTGCGCGTACCGCTTCAATGGTGGTGCCTCCCGGTGAGCAGACCATATCtt                              <  2:82662/81‑1 (MQ=255)                                               aCTGCAGCACGGAAGCCTTTCTCTTCCAGTGCGCGTACCGCTTCAATGGTGGTGCCTCCCGGTGAGCAGACCATATCttt                             <  2:82663/80‑1 (MQ=255)                                                  gcagcaCGGAAGCCTTTCTCTTCCAGTGCGCGTACCGCTTCAATGGTGGTGCCTCCCGGTGAGCAGACCATATCTTTCAGt                         >  1:82700/1‑81 (MQ=255)                                                    agcaCGGAAGCCTTTCTCTTCCAGTGCGCGTACCGCTTCAATGGTGGTGCCTCCCGGTGAGCAGACCATATCTTTCAGTGc                       >  2:82701/1‑81 (MQ=255)                                                         ggAAGCCTTTCTCTTCCAGTGCGAGTACCGCATAAATGGTGGTGCATCCCGGTGAGCAGACCATATCTTTCAGTGCCCCCg                  <  1:897904/81‑1 (MQ=255)                                                             gCCTTTCTCTTCCAGTGCGCGTACCGCTTCAATGGTGGTGCCTCCCGGTGAGCAGACCATATCTTTCAGTGCCCCCGGATg              >  1:82697/1‑81 (MQ=255)                                                                      ttCCAGTGCGCGTACCGCTTCAATGGTGGTGCCTCCCGGTGAGCAGACCATATCTTTCAGTGCCCCCGGATGTTCTccc       <  2:82664/79‑1 (MQ=255)                                                                        ccAGTGCGCGTACCGCTTCAATGGT‑GTGCCTCCCGGTGAGCAGACCATATCTTTCAGTGCCCCCGGATGTTCTCCCGTTTc  >  1:82706/1‑81 (MQ=255)                                                                         cAGTGCGCGTACCGCTTCAATGGTGGTGCCTCCCGGTGAGCAGACCATATCTTTCAGTGCCCCCGGATGTTCTCCCGTTTc  <  2:82665/81‑1 (MQ=255)                                                                             |                                                                             GCTGAGTTTTTCTGATTTTTCCATACACTTCGTCATCGCTTCGATCACTGCAGCACGGAAGCCTTTCTCTTCCAGTACGCGTACCGCTTCAATGGTGGTGCCTCCCGGTGAGCAGACCATATCTTTCAGTGCCCCCGGATGTTCTCCCGTTTC  >  NC_000913/404844‑404996

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑