Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,339,401	T→C	N374S (AAC→AGC)	adiA ←	arginine decarboxylase, degradative

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,339,401	0	T	C	100.0%	134.6 / NA	39	N374S (AAC→AGC)	adiA	arginine decarboxylase, degradative
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (20/19);  total (20/19)

CGTACATGAATATAAGAAGCCTGTGACAGCGCATTCAGCAGTTTGTGGGTGGAGTGGGTGGCGAAAACGGTAGGACCGTTGTGATCGCCAGGTTCGCCGCGCATGGCATAGTGATCGGCATAGATCGGGTTGAAACGTGCATAGCCGTACC  >  NC_000913/4339323‑4339473                                                                               |                                                                         cGTACATGAATATAAGAAGCCTGTGACAGCGCATTCAGCAGTTTGTGGGTGGAGTGGGTGGCGAAAACGGTAGGACCGCtg                                                                        >  1:823919/1‑81 (MQ=255)  gTACATGAATATAAGAAGCCTGTGACAGCGCATTCAGCAGTTTGTGGGTGGAGTGGGTGGCGAAAACGGTAGGACCGCtgt                                                                       >  2:823904/1‑81 (MQ=255)   tACATGAATATAAGAAGCCTGTGACAGCGCATTCAGCAGTTTGTGGGTGGAGTGGGTGGCGAAAACGGTAGGACCGCtgtg                                                                      <  2:823849/81‑1 (MQ=255)     cATGAATATAAGAAGCCTGTGACAGCGCATTCAGCAGTTTGTGGGTGGAGTGGGTGGCGAAAACGGTAGGACCGCTGTGAt                                                                    <  1:823851/81‑1 (MQ=255)         aaTATAAGAAGCCTGTGACAGCGCATTCAGCAGTTTGTGGGTGGGGTGGGTGGCGAAAACGGTAGGACCGCTGTGATCGcc                                                                <  2:823852/81‑1 (MQ=255)         aaTATAAGAAGCCTGTGACAGCGCATTCAGCAGTTTGTGGGTGGAGTGGGTGGCGAAAACGGTAGGACCGCTGTGATCGcc                                                                <  1:823852/81‑1 (MQ=255)         aaTATAAGAAGCCTGTGACAGCGCATTCAGCAGTTTGTGGGTGGAGTGGGTGGCGAAAACGGTAGGACCGCTGTGATCGc                                                                 >  2:823893/1‑80 (MQ=255)          atatAAGAAGCCTGTGACAGCGCATTCAGCAGTTTGTGGGTGGAGTGGGTGGCGAAAACGGTAGGACCGCTGTGATCGCCa                                                               <  1:823854/81‑1 (MQ=255)             taAGAAGCCTGTGACAGCGCATTCAGCAGTTTGTGGGTGGAGTGGGTGGCGAAAACGGTAGGACCGCTGTGATCGCCAgg                                                             <  2:823854/80‑1 (MQ=255)             taAGAAGCCTGTGACAGCGCATTCAGCAGTTTGTGGGTGGAGTGGGTGGCGAAAACGGTAGGACCGCTGTGATCGCCAGGt                                                            <  1:823856/81‑1 (MQ=255)             taAGAAGCCTGTGACAGCGCATTCAGCAGTTTGTGGGTGGAGTGGGTGGCGAAAACGGTAGGACCGCTGTGATCGCCAGGt                                                            >  1:823904/1‑81 (MQ=255)               agaagCCTGTGACAGCGCATTCAGCAGTTTGTGGGTGGAGTGGGTGGCGAAAACGGTAGGACCGCTGTGATCGCCAGGTTc                                                          <  1:823857/81‑1 (MQ=255)                 aagCCTGTGACAGCGCATTCAGCAGTTTGTGGGTGGAGTGGGTGGCGAAAACGGTAGGACCGCTGTGATCGCCAGGTTcgc                                                        >  2:823926/1‑81 (MQ=255)                  agCCTGTGACAGCGCATTCAGCAGTTTGTGGGTGGAGTGGGTGGCGAAAACGGTAGGACCGCTGTGATCGCCAGGTTcgcc                                                       <  2:823857/81‑1 (MQ=255)                        tgACAGCGCATTCAGCAGTTTGTGGGTGGAGTGGGTGGCGAAAACGGTAGGACCGCTGTGATCGCCAGGTTCGCCgcgc                                                   <  1:823859/79‑1 (MQ=255)                        tgACAGCGCATTCAGCAGTTTGTGGGTGGAGTGGGTGGCGAAAACGGTAGGACCGCTGTGATCGCCAGGTTCGCCGCGCa                                                  <  2:823859/80‑1 (MQ=255)                         gACAGCGCATTCAGCAGTTTGTGGGTGGAGTGGGTGGCGAAAACGGTAGGACCGCTGTGATCGCCAGGTTCGCCGCGCATg                                                >  2:823914/1‑81 (MQ=255)                            aGCGCATTCAGCAGTTTGTGGGTGGAGTGGGTGGCGAAAACGGTAGGACCGCTGTGATCGCCAGGTTCGCCGCGCATGGCa                                             >  2:823917/1‑81 (MQ=255)                                 aTTCAGCAGTTTGTGGGTGGAGTGGGTGGCGAAAACGGTAGGACCGCTGTGATCGCCAGGTTCGCCGCGCATGGCATAGTg                                        <  1:823861/81‑1 (MQ=255)                                    cagcagTTTGTGGGTGGAGTGGGTGGCGAAAACGGTAGGACCGCTGTGATCGCCAGGTTCGCCGCGCATGGCATAGTGATc                                     >  2:823909/1‑81 (MQ=255)                                      gcagTTTGTGGGTGGAGTGGGTGGCGAAAACGGTAGGACCGCTGTGATCGCCAGGTTCGCCGCGCATGGCATAGTGATCgg                                   >  2:823920/1‑81 (MQ=255)                                        agTTTGTGGGTGGAGTGGGTGGCGAAAACGGTAGGACCGCTGTGATCGCCAGGTTCGCCGCGCATGGCATAGTGATCGGCa                                 >  2:823911/1‑81 (MQ=255)                                          tttGTGGGTGGAGTGGGTGGCGAAAACGGTAGGACCGCTGTGATCGCCAGGTTCGCCGCGCATGGCATAGTGATCGGCa                                 >  2:823923/1‑79 (MQ=255)                                             gtgggtggAGTGGGTGGCGAAAACGGTAGGACCGCTGTGATCGCCAGGTTCGCCGCGCATGGCATAGTGATCGGCATAGa                             <  1:823862/80‑1 (MQ=255)                                                 gtggAGTGGGTGGCGAAAACGGTAGGACCGCTGTGATCGCCAGGTTCGCCGCGCATGGCATAGTGATCGGCATAGATCggg                        >  2:823919/1‑81 (MQ=255)                                                 gtggAGTGGGTGGCGAAAACGGTAGGACCGCTGTGATCGCCAGGTTCGCCGCGCATGGCATAGTGATCGGCATAGATCggg                        >  2:823912/1‑81 (MQ=255)                                                   ggAGTGGGTGGCGAAAACGGTAGGACCGCTGTGATCGCCAGGTTCGCCGCGCATGGCATAGTGATCGGCATAGATCGGGtt                      >  2:823907/1‑81 (MQ=255)                                                   ggAGTGGGTGGCGAAAACGGTAGGACCGCTGTGATCGCCAGGTTCGCCGCGCATGGCATAGTGATCGGCATAGATCGGGtt                      >  2:823913/1‑81 (MQ=255)                                                    gAGTGGGTGGCGAAAACGGTAGGACCGCTGTGATCGCCAGGTTCGCCGCGCATGGCATAGTGATCGGCATAGATCGGGTTg                     <  1:823863/81‑1 (MQ=255)                                                      gtgggtggCGAAAGCGGTAGGACCGCTGTGATCGCCAGGTTCGCCGCGCATGGCATAGTGATCGGCATAGATCGGGTTGaa                   <  1:823864/81‑1 (MQ=255)                                                         ggtggCGAAAACGGTAGGACCGCTGTGATCGCCAGGTTCGCCGCGCATGGCATAGTGATCGGCATAGATCGGGTTGAAACg                >  2:823908/1‑81 (MQ=255)                                                         ggtggCGAAAACGGTAGGACCGCTGTGATCGCCAGGTTCGCCGCGCATGGCATAGTGATCGGCATAGATCGGGTTGAAACg                >  1:823926/1‑81 (MQ=255)                                                                aaaaCGGTAGGACCGCTGTGATCGCCAGGTTCGCCGCGCATGGCATAGTGATCGGCATAGATCGGGTTGAAACGTGCATAg         <  2:823864/81‑1 (MQ=255)                                                                 aaaCGGTAGGACCGCTGTGATCGCCAGGTTCGCCGCGCATGGCATAGTGATCGGCATAGATCGGGTTGAAACGTGCATAGc        <  2:823866/81‑1 (MQ=255)                                                                 aaaCGGTAGGACCGCTGTGATCGCAAGGTTCGCCGCGCATGGCATAGTGATCGGCATAGATCGGGTTGAAACGTGCATAGc        <  2:823865/81‑1 (MQ=255)                                                                  aaCGGTAGGACCGCTGTGATCGCCAGGTTCGCCGCGCATGGCATAGTGATCGGCATAGATCGGGTTGAAACGTGCATAGcc       >  1:823925/1‑81 (MQ=255)                                                                      gTAGGACCGCTGTGATCGCCAGGTTCGCCGCGCATGGCATAGTGATCGGCATAGATCGGGTTGAAACGTGCATAGCCGTAc   >  2:823927/1‑81 (MQ=255)                                                                       tAGGACCGCTGTGATCGCCAGGTTCGCCGCGCATGGCATAGTGATCGGCATAGATCGGGTTGAAACGTGCATAGCCGTAcc  <  1:823868/81‑1 (MQ=255)                                                                        aGGACCGCTGTGATCGCCAGGTTCGCCGCGCATGGCATAGTGATCGGCATAGATCGGGTTGAAACGTGCATAGCCGTAc   >  2:823916/1‑79 (MQ=255)                                                                               |                                                                         CGTACATGAATATAAGAAGCCTGTGACAGCGCATTCAGCAGTTTGTGGGTGGAGTGGGTGGCGAAAACGGTAGGACCGTTGTGATCGCCAGGTTCGCCGCGCATGGCATAGTGATCGGCATAGATCGGGTTGAAACGTGCATAGCCGTACC  >  NC_000913/4339323‑4339473

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑