| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,343,621 | T→C | L85L (TTG→CTG) | melB → | melibiose:H(+)/Na(+)/Li(+) symporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,343,621 | 0 | T | C | 97.1% | 111.7 / ‑3.9 | 34 | L85L (TTG→CTG) | melB | melibiose:H(+)/Na(+)/Li(+) symporter |
| Reads supporting (aligned to +/- strand): ref base T (0/1); new base C (10/23); total (10/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.56e-01 | |||||||||||
GATCCGATTATGGGATGGATTGTAAATGCTACGCGATCGCGATGGGGTAAGTTCAAACCCTGGATCCTGATCGGTACGTTGGCAAACTCTGTAATCTTATTTCTCCTCTTTAGTGCGCATCTGTTTGAAGGTACTACTCAGATTGTCTTTGTTT > NC_000913/4343543‑4343696 | gATCCGATTATGGGATGGATTGTAAATGCTACGCGATCGCGATGGGGTAAGTTCAAACCCTGGATCCTGATCGGTACGCTg > 2:824867/1‑81 (MQ=255) tCCGATTATGGGATGGATTGTAAATGCTACGCGATCGCGATGGGGTAAGTTCAAACCCTGGATCCTGATCGGTACGCTGGc < 1:824829/81‑1 (MQ=255) cGATTATGGGATGGATTGTAAATGCTACGCGATCGCGATGGGGTAAGTTCAAACCCTGGATCCTGATCGGTACGCTGGCaa < 2:824829/81‑1 (MQ=255) tGGGATGGATTGTAAATGCTACGCGATCGCGATGGGGTAAGTTCAAACCCTGGATCCTGATCGGTACGCTGGCAAActct < 2:824830/80‑1 (MQ=255) gggATGGATTGTAAATGCTACGCGATCGCGATGGGGTAAGTTCAAACCCTGGATCCTGATCGGTACGCTGGCAAACTCTg > 1:824875/1‑80 (MQ=255) gatggatTGTAAATGCTACGCGATCGCGATGGGGTAAGTTCAAACCCTGGATCCTGATCGGTACGCTGGCAAACTCTGTaa < 1:824832/81‑1 (MQ=255) gatTGTAAATGCTACGCGATCGCGATGGGGTAAGTTCAAACCCTGGATCCTGATCGGTACGCTGGCAAACTCTGTAATCtt < 1:824833/81‑1 (MQ=255) tAAATGCTACGCGATCGCGATGGGGTAAGTTCAAACCCTGGATCCTGATCGGTACGCTGGCAAACTCTGTAATCTTATTtc < 1:824834/81‑1 (MQ=255) cgcgatcgcgatGGGGTAAGTTCAAACCCTGGATCCTGATCGGTACGCTGGCAAACTCTGTAATCTTATTTCTCCTCttt < 2:824834/80‑1 (MQ=255) cgcgatcgcgatGGGGTAAGTTCAAACCCTGGATCCTGATCGGTACGCTGGCAAACTCTGTAATCTTATTTCTCCTCttt < 1:824836/80‑1 (MQ=255) atcgcgatGGGGTAAGTTCAAACCCTGGATCCTGATCGGTACGCTGGCAAACTCTGTAATCTTATTTCTCCTCTTTAGTgc > 1:824903/1‑81 (MQ=255) tcgcgatGGGGTAAGTTCAAACCCTGGATCCTGATCGGTACGCTGGCAAACTCTGTAATCTTATTTCTCCTCTTTAGTgcg < 2:824836/81‑1 (MQ=255) gatGGGGTAAGTTCAAACCCTGGATCCTGATCGGTACGCTGGCAAACTCTGTAATCTTATTTCTCCTCTTTAGTGCGCATc > 2:824880/1‑81 (MQ=255) gatGGGGTAAGTTCAAACCCTGGATCCTGATCGGTACGCTGGCAAACTCTGTAATCTTATTTCTCCTCTTTAGTGCGCATc < 1:824838/81‑1 (MQ=255) tGGGGTAAGTTCAAACCCTGGATCCTGATCGGTACGCTGGCAAACTCTGTAATCTTATTTCTCCTCTTTAGTGCGCATCTg < 2:824838/81‑1 (MQ=255) gggTAAGTTCAAACCCTGGATCCTGATCGGTACGCTGGCAAACTCTGTAATCTTATTTCTCCTCTTTAGTGCGCATCTGtt < 1:824840/81‑1 (MQ=255) ggTAAGTTCAAACCCTTGATCCTGATCGGTACGCTGGCAAACTCTGTAATCTTATTTCTCCTCTTTAGTGCGCATCTGttt < 2:824840/81‑1 (MQ=255) ggTAAGTTCAAACCCTGGATCCTGATCGGTACGTTGGCAAACTCTGTAATCTTATTTCTCCTCTTTAGTGCGCATCTGttt < 2:824841/81‑1 (MQ=255) aGTTCAAACCCTGGATCCTGATCGGTACGCTGGCAAACTCTGTAATCTTATTTCTCCTCTTTAGTGCGCATCTGTTTGAAg < 1:824843/81‑1 (MQ=255) aGTTCAAACCCTGGATCCTGATCGGTACGCTGGCAAACTCTGTAATCTTATTTCTCCTCTTTAGTGCGCATCTGTTTGAAg < 2:824843/81‑1 (MQ=255) gTTCAAACCCTGGATCCTGATCGGTACGCTGGCAAACTCTGTAATCTTATTTCTCCTCTTTAGTGCGCATCTGTTTGAAgg > 2:824877/1‑81 (MQ=255) cAAACCCTGGATCCTGATCGGTACGCTGGCAAACTCTGTAATCTTATTTCTCCTCTTTAGTGCTCATCTGTTTGAAGGta < 2:824844/80‑1 (MQ=255) aCCCTGGATCCTGATCGGTACGCTGGCAAACTCTGTAATCTTATTTCTCCTCTTTAGTGCGCATCTGTTTGAAGGtactac < 1:824846/81‑1 (MQ=255) cccTGGATCCTGATCGGTACGCTGGCAAACTCTGTAATCTTATTTCTCCTCTTTAGTGCGCATCTGTTTGAAGGTactact < 2:824846/81‑1 (MQ=255) tGGATCCTGATCGGTACGCTGGCAAACTCTGTAATCTTATTTCTCCTCTTTAGTGCGCATCTGTTTGAAGGTactact > 1:824883/1‑78 (MQ=255) tGGATCCTGATCGGTACGCTGGCAAACTCTGTAATCTTATTTCTCCTCTTTAGTGCGCATCTGTTTGAAGGTactact > 2:824866/1‑78 (MQ=255) ggATCCTGATCGGTACGCTGGCAAACTCTGTAATCTTATTTCTCCTCTTTAGTGCGCATCTGTTTGAAGGTactact > 2:824887/1‑77 (MQ=255) gATCCTGATCGGTACGCTGGCAAACTCTGTAATCTTATTTCTCCTCTTTAGTGCGCATCTGTTTGAAGGTACTACTCAGAt < 2:824847/81‑1 (MQ=255) gATCCTGATCGGTACGCTGGCAAACTCTGTAATCTTATTTCTCCTCTTTAGTGCGCATCTGTTTGAAGGTACTACTCAGAt < 1:824849/81‑1 (MQ=255) cTGATCGGTACGCTGGCAAACTCTGTAATCTTATTTCTCCTCTTTAGTGCGCATCTGTTTGAAGGTACTACTCAGATTGTc < 1:824850/81‑1 (MQ=255) gATCGGTACGCTGGCAAACTCTGTAATCTTATTTCTCCTCTTTAGTGCGCATCTGTTTGAAGGTACTACTCAGATTGTCtt > 2:824888/1‑81 (MQ=255) aTCGGTACGCTGGCAAACTCTGTAATCTTATTTCTCCTCTTTAGTGCGCATCTGTTTGAAGGTACTACTCAGATTGTCt < 2:824850/79‑1 (MQ=255) ggTACGCTGGCAAACTCTGTAATCTTATTTCTCCTCTTTAGTGCGCATCTGTTTGAAGGTACTACTCAGATTGTCtttgtt > 1:824887/1‑81 (MQ=255) gTACGCTGGCAAACTCTGTAATCTTATTTCTCCTCTTTAGTGCGCATCTGTTTGAAGGTACTACTCAGATTGTCtttgttt < 2:824851/81‑1 (MQ=255) | GATCCGATTATGGGATGGATTGTAAATGCTACGCGATCGCGATGGGGTAAGTTCAAACCCTGGATCCTGATCGGTACGTTGGCAAACTCTGTAATCTTATTTCTCCTCTTTAGTGCGCATCTGTTTGAAGGTACTACTCAGATTGTCTTTGTTT > NC_000913/4343543‑4343696 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |