Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,347,470	C→T	G425G (GGG→GGA)	dcuB ←	anaerobic C4‑dicarboxylate transporter DcuB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,347,470	0	C	T	100.0%	95.3 / NA	28	G425G (GGG→GGA)	dcuB	anaerobic C4‑dicarboxylate transporter DcuB
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (17/11);  total (17/11)

CGCAAAGTGCATTTATAAGAACCCGTACATCGCGGCGAAGATCCAGCCGAAGACGCACGATACGCTCACACCAATCAACCCCGGCAGAATAAAGCTGTGGTTGATGACGAAGCGACCGATGTGGGTGGTGCCGGAACGGTCAAACTGAATC  >  NC_000913/4347392‑4347542                                                                               |                                                                         cGCAAAGTGCATTTATAAGAACCCGTACATCGCGGCGAAGATCCAGCCGAAGACGCACGATACGCTCACACCAATCAAtcc                                                                        >  2:825799/1‑81 (MQ=255)  gCAAAGTGCATTTATAAGAACCCGTACATCGCGGCGAAGATCCAGCCGAAGACGCACGATACGCTCACACCAATCAAtccc                                                                       <  1:825753/81‑1 (MQ=255)    aaaGTGCATTTATAAGAACCCGTACATCGCGGCGAAGATCCAGCCGAAGACGCACGATACGCTCACACCAATCAATCCCgg                                                                     >  2:825810/1‑81 (MQ=255)               ataAGAACCCGTACATCGCGGCGAAGATCCAGCCGAAGACGCACGATACGCTCACACCAATCAATCCCGGCAGAATAAAGc                                                          >  2:825811/1‑81 (MQ=255)                taAGAACCCGTACATCGCGGCGAAGATCCAGCCGAAGACGCACGATACGCTCACACCAATCAATCCCGGCAGAATAAAGCt                                                         <  2:825753/81‑1 (MQ=255)                       ccGTACATCGCGGCGAAGATCCAGCCGAAGACGCACGATACGCTCACACCAATCAATCCCGGCAGAATAAAGCTGTGGtt                                                   >  1:825809/1‑80 (MQ=255)                       ccGTACATCGCGGCGAAGATCCAGCCGAAGACGCACGATACGCTCACACCAATCAATCCCGGCAGAATAAAGCTGTGGTtg                                                  <  1:825755/81‑1 (MQ=255)                          tACATCGCGGCGAAGATCCAGCCGAAGACGCACGATACGCTCACACCAATCAATCCCGGCAGAATAAAGCTGTGGTtgat                                                >  2:825819/1‑80 (MQ=255)                            cATCGCGGCGAAGATCCAGCCGAAGACGCACGATACGCTCACACCAATCAATCCCGGCAGAATAAAGCTGTGGTTGATGAc                                             <  2:825755/81‑1 (MQ=255)                              tCGCGGCGAAGATCCAGCCGAAGACGCACGATACGCTCACACCAATCAATCCCGGCAGAATAAAGCTGTGGTTGATGACGa                                           <  2:825756/81‑1 (MQ=255)                              tCGCGGCGAAGATCCAGCCGAAGACGCACGATACGCTCACACCAATCAATCCCGGCAGAATAAAGCTGTGGTTGATGACGa                                           <  2:825757/81‑1 (MQ=255)                                gcggcgAAGATCCAGCCGAAGACGCACGATACGCTCACACCAATCAATCCCGGCAGAATAAAGCTGTGGTTGATGACGAAg                                         >  1:825807/1‑81 (MQ=255)                                   gcgAAGATCCAGCCGAAGACGCACGATACGCTCACACCAATCAATCCCGGCAGAATAAAGCTGTGGTTGATGACGAAGCGa                                      >  1:825803/1‑81 (MQ=255)                                   gcgAAGATCCAGCCGAAGACGCACGATACGCTCACACCAATCAATCCCGGCAGAATAAAGCTGTGGTTGATGACGAAGCGa                                      <  1:825759/81‑1 (MQ=255)                                    cgAAGATCCAGCCGAAGACGCACGATACGCTCACACCAATCAATCCCGGCAGAATAAAGCTGTGGTTGATGACGAAGCGAc                                     >  1:825808/1‑81 (MQ=255)                                    cgAAGATCCAGCCGAAGACGCACGATACGCTCACACCAATCAATCCCGGCAGAATAAAGCTGTGGTTGATGACGAAGCGAc                                     >  1:825802/1‑81 (MQ=255)                                       aGATCCAGCCGAAGACGCACGATACGCTCACACCAATCAATCCCGGCAGAATAAAGCTGTGGTTGATGACGAAGCGACCGa                                  <  2:825759/81‑1 (MQ=255)                                        gATCCAGCCGAAGACGCACGATACGCTCACACCAATCAATCCCGGCAGAATAAAGCTGTGGTTGATGACGAAGCGACCGAt                                 >  2:825805/1‑81 (MQ=255)                                         aTCCAGCCGAAGACGCACGATACGCTCACACCAATCAATCCCGGCAGAATAAAGCTGTGGTTGATGACGAAGCGACCGAtg                                >  2:825813/1‑81 (MQ=255)                                           ccAGCCGAAGACGCACGATACGCTCACACCAATCAATCCCGGCAGAATAAAGCTGTGGTTGATGACGAAGCGACCGAtgtg                              >  1:825805/1‑81 (MQ=255)                                            cAGCCGAAGACGCACGATACGCTCACACCAATCAATCCCGGCAGAATAAAGCTGTGGTTGATGACGAAGCGACCGAtgtg                              <  2:825760/80‑1 (MQ=255)                                                cGAAGACGCACGATACGCTCACACCAATCAATCCCGGCAGAATAAAGCTGTGGTTGATGACGAAGCGACCGATgtgggtgg                         <  1:825762/81‑1 (MQ=255)                                                    gACGCACGATACGCTCACACCAATCAATCCCGGCAGAATAAAGCTGTGGTTGATGACGAAGCGACCGATGTGGGTGGTGcc                     <  1:825763/81‑1 (MQ=255)                                                      cGCACGATACGCTCACACCAATCAATCCCGGCAGAATAAAGCTGTGGTTGATGACGAAGCGACCGATGTGGGTGGTGCCgg                   <  2:825763/81‑1 (MQ=255)                                                      cGCACGATACGCTCACACCAATCAATCCCGGCAGAATAAAGCTGTGGTTGATGACGAAGCGACCGATGTGGGTGGTGCCg                    >  2:825815/1‑80 (MQ=255)                                                       gCACGATACGCTCACACCAATCAATCCCGGCAGAATAAAGCTGTGGTTGATGACGAAGCGACCGATGTGGGTGGTGCCgg                   >  2:825824/1‑80 (MQ=255)                                                        cACGATACGCTCACACCAATCAATCCCGGCAGAATAAAGCTGTGGTTGATGACGAAGCGACCGATGTGGGTGGTGCCGGaa                 >  1:825822/1‑81 (MQ=255)                                                            aTACGCTCACACCAATCAATCCCGGCAGAATAAAGCTGTGGTTGATGACGAAGCGACCGATGTGGGTGGTGCCGGAACGGt             >  1:825765/1‑81 (MQ=255)                                                                      acCAATCAATCCCGGCAGAATAAAGCTGTGGTTGATGACGAAGCGACCGATGTGGGTGGTGCCGGAACGGTCAAACTGAAt   >  1:825824/1‑81 (MQ=255)                                                                       ccAATCAATCCCGGCAGAATAAAGCTGTGGTTGATGACGAAGCGACCGATGTGGGTGGTGCCGGAACGGTCAAACTGAATc  >  1:825823/1‑81 (MQ=255)                                                                               |                                                                         CGCAAAGTGCATTTATAAGAACCCGTACATCGCGGCGAAGATCCAGCCGAAGACGCACGATACGCTCACACCAATCAACCCCGGCAGAATAAAGCTGTGGTTGATGACGAAGCGACCGATGTGGGTGGTGCCGGAACGGTCAAACTGAATC  >  NC_000913/4347392‑4347542

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑