Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,371,109	G→A	V29I (GTT→ATT)	groL →	chaperonin GroEL

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,371,109	0	G	A	100.0%	110.0 / NA	31	V29I (GTT→ATT)	groL	chaperonin GroEL
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (12/19);  total (12/19)

AAGACGTAAAATTCGGTAACGACGCTCGTGTGAAAATGCTGCGCGGCGTAAACGTACTGGCAGATGCAGTGAAAGTTACCCTCGGTCCAAAAGGCCGTAACGTAGTTCTGGATAAATCTTTCGGTGCACCGACCATCACCAAAGATGGTGTT  >  NC_000913/4371035‑4371186                                                                           |                                                                              aaGACGTAAAATTCGGTAACGACGCTCGTGTGAAAATGCTGCGCGGCGTAAACGTACTGGCAGATGCAGTGAAAATTAc                                                                           <  2:831055/79‑1 (MQ=255) aaGACGTAAAATTCGGTAACGACGCTCGTGTGAAAATGCTGCGCGGCGTAAACGTACTGGCAGATGCAGTGAAAATTAc                                                                           <  1:831058/79‑1 (MQ=255) aaGACGTAAAATTCGGTAACGACGCTCGTATGAAAATGCTGCGCGGCGTAAACGTACTGGCAGATGCAGTGAAAATTAc                                                                           <  2:831056/79‑1 (MQ=255)  aGACGTAAAATTCGGTAACGACGCTCGTGTGAAAATGCTGCGCGGCGTAAACGTACTGGCAGATGCAGTGAAAATTACCCt                                                                        >  2:831126/1‑81 (MQ=255)     cGTAAAATTCGGTAACGACGCTCGTGTGAAAATGCTGCGCGGCGTAAACGTACTGGCAGATGCAGTGAAAATTACCCTCgg                                                                     >  1:831121/1‑81 (MQ=255)      gTAAAATTCGGTAACGACGCTCGTGTGAAAATGCTGCGCGGCGTAAACGTACTGGCAGATGCAGTGAAAATTACCCTCGGt                                                                    <  2:831058/81‑1 (MQ=255)      gTAAAATTCGGTAACGACGCTCGTGTGAAAATGCTGCGCGGCGTAAACGTACTGGCAGATGCAGTGAAAATTACCCTCGGt                                                                    >  1:831110/1‑81 (MQ=255)         aaaTTCGGTAACGACGCTCGTGTGAAAATGCTGCGCGGCGTAAACGTACTGGCAGATGCAGTGAAAATTACCCTCGGTcc                                                                  <  2:831059/80‑1 (MQ=255)                    cgacgCTCGTGTGAAAATGCTGCGCGGCGTAAACGTACTGGCAGATGCAGTGAAAATTACCCTCGGTCCAAAAGGCCGTaa                                                      <  1:831061/81‑1 (MQ=255)                    cgacgCTCGTGTGAAAATGCTGCGCGGCGTAAACGTACTGGCAGATGCAGTGAAAATTACCCTCGGTCCAAAAGGCCGTaa                                                      <  1:831062/81‑1 (MQ=255)                     gacgCTCGTGTGAAAATGCTGCGCGGCGTAAACGTACTGGCAGATGCAGTGAAAATTACCCTCGGTCCAAAAGGCCGTAAc                                                     <  1:831063/81‑1 (MQ=255)                        gCTCGTGTGAAAATGCTGCGCGGCGTAAACGTACTGGCAGATGCAGTGAAAATTACCCTCGGTCCAAAAGGCCGTAACg                                                    >  1:831124/1‑79 (MQ=255)                           cGTGTGAAAATGCTGCGCGGCGTAAACGTACTGGCAGATGCAGTGAAAATTACCCTCGGTCCAAAAGGCCGTAACGTAGtt                                               >  1:831108/1‑81 (MQ=255)                            gtgtGAAAATGCTGCGCGGCGTAAACGTACTGGCAGATGCAGTGAAAATTACCCTCGGTCCAAAAGGCCGTAACGTAGtt                                               <  1:831064/80‑1 (MQ=255)                              gtgAAAATGCTGCGCGGCGTAAACGTACTGGCAGATGCAGTGAAAATTACCCTCGGTCCAAAAGGCCGTAACGTAGTTCTg                                            >  2:831124/1‑81 (MQ=255)                                  aaaTGCTGCGCGGCGTAAACGTACTGGCAGATGCAGTGAAAATTACCCTCGGTCCAAAAGGCCGTAACGTAGTTCTGGATa                                        <  2:831064/81‑1 (MQ=255)                                     tgctgcGCGGCGTAAACGTACTGGCAGATGCAGTGAAAATTACCCTCGGTCCAAAAGGCCGTAACGTAGTTCTGGATAAAt                                     <  1:831066/81‑1 (MQ=255)                                       ctgcGCGGCGTAAACGTACTGGCAGATGCAGTGAAAATTACCCTCGGTCCAAAAGGCCGTAACGTAGTTCTGGATAAATc                                    <  1:831067/80‑1 (MQ=255)                                          cgcgGCGTAAACGTACTGGCAGGTGCAGTGAAAATTACCCTCGGTCCAAAAGGCCGTAACGTAGTTCTGGATAAGTCTTTc                                <  1:831068/81‑1 (MQ=255)                                           gcggcgTAAACGTACTGGCAGATGCAGTGAAAATTACCCTCGGTCCAAAAGGCCGTAACGTAGTTCTGGATAAATCTTTCg                               >  2:831100/1‑81 (MQ=255)                                                gTAAACGTACTGGCAGATGCAGTGAAAATTACCCTCGGTCCAAAAGGCCGTAACGTAGTTCTGGATAAATCTTTCGGTGCa                          <  1:831069/81‑1 (MQ=255)                                                gTAAACGTACTGGCAGATGCAGTGAAAATTACCCTCGGTCCAAAAGGCCGTAACGTAGTTCTGGATAAATCTTTCGGTGCa                          >  2:831113/1‑81 (MQ=255)                                                   aaCGTACTTGCAGATGCAGTGAAAATTACCCTCGGTCCAAAAGGCCGTAACGTAGTTCTGGATAAATCTTTCGGTGCAcc                        <  1:831070/80‑1 (MQ=255)                                                    aCGTACTGGCAGATGCAGTGAAAATTACCCTCGGTCCAAAAGGCCGTAACGTAGTTCTGGATAAATCTTTCGGTGCACCGa                      <  1:831071/81‑1 (MQ=255)                                                    aCGTACTGGCAGATGCAGTGAAAATTACCCTCGGTCCAAAAGGCCGTAACGTAGTTCTGGATAAATCTTTCGGTGCACCGa                      >  2:831139/1‑81 (MQ=255)                                                      gTACTGGCAGATGCAGTGAAAATTACCCTCGGTCCAAAAGGCCGTAACGTAGTTCTGGATAAATCTTTCGGTGCACCGAcc                    <  1:831072/81‑1 (MQ=255)                                                        aCTGGCAGATGCAGTGAAAATTACCCTCGGTCCAAAAGGCCGTAACGTAGTTCTGGATAAATCTTTCGGTGCACCGACCAt                  >  2:831116/1‑81 (MQ=255)                                                         cTGGCAGATGCAGTGAAAATTACCCTCGGTCCAAAAGGCCGTAACGTAGTTCTGGATAAATCTTTCGGTGCACCGACCATc                 <  1:831073/81‑1 (MQ=255)                                                          tGGCAGATGCAGTGAAAATTACCCTCGGTCCAAAAGGCCGTAACGTAGTTCTGGATAAATCTTTCGGTGCACCGACCATCa                >  1:831130/1‑81 (MQ=255)                                                                   cAGTGAAAATTACCCTCGGTCCAAAAGGCCGTAACGTAGTTCTGGATAAATCTTTCGGTGCACCGACCATCACCAAAGAt        <  2:831073/80‑1 (MQ=255)                                                                     gTGAAAATTACCCTCGGTCCAAAAGGCCGTAACGTAGTTCTGGATAAATTTTGCGGTGCACCGACCATCACCAAAGATgtt     >  2:831115/1‑79 (MQ=255)                                                                        aaaaTTACCCTCGGTCCAAAAGGCCGTAACGTAGTTCTGGATAAATCTTTCGGTGCACCGACCATCACCAAAGATGGTGtt  >  2:831134/1‑81 (MQ=255)                                                                           |                                                                              AAGACGTAAAATTCGGTAACGACGCTCGTGTGAAAATGCTGCGCGGCGTAAACGTACTGGCAGATGCAGTGAAAGTTACCCTCGGTCCAAAAGGCCGTAACGTAGTTCTGGATAAATCTTTCGGTGCACCGACCATCACCAAAGATGGTGTT  >  NC_000913/4371035‑4371186

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑