Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,390,171	T→C	N64D (AAC→GAC)	psd ←	phosphatidylserine decarboxylase proenzyme

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,390,171	0	T	C	100.0%	90.3 / NA	26	N64D (AAC→GAC)	psd	phosphatidylserine decarboxylase proenzyme
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (9/17);  total (9/17)

AGGCATGACCAGTACATTCGGATCGGTATCGATTGGGCGTACTTCGTCACGCAGCGGACGGACAAAGAATTCGTTAAAGGTGCGGTAGCTGGCGGTGTCCGGCTTTTGCGCCTCTTTCATGTCGACCTTGTAGTATTTAACGAACAGATCGA  >  NC_000913/4390097‑4390248                                                                           |                                                                              aggcaTGACCAGTACATTCGGATCGGTATCGATTGGGCGTACTTCGTCACGCAGCGGACGGACAAAGAATTCGTCAAAGGt                                                                         >  1:835719/1‑81 (MQ=255)  ggcaTGACCAGTACATTCGGATCGGTATCGATTGGGCGTACTTCGTCACGCAGCGGACGGACAAAGAATTCGTCAAAGGTg                                                                        >  2:835711/1‑81 (MQ=255)   gcaTGACCAGTACATTCGGATCGGTATCGATTGGGCGTACTTCGTCACGCAGCGGACGGACAAAGAATTCGTCAAAGGTGc                                                                       <  2:835677/81‑1 (MQ=255)   gcaTGACCAGTACATTCGGATCGGTATCGATTGGGCGTACTTCGTCACGCAGCGGACGGACAAAGAATTCGTCAAAGGTGc                                                                       <  1:835677/81‑1 (MQ=255)         ccAGTACATTCGGATCGGTATCGATTGGGCGTACTTCGTCACGCAGCGGACGGACAAAGAATTCGTCAAAGGTGCGGTAGc                                                                 <  1:835679/81‑1 (MQ=255)               cATTCGGATCGGTATCGATTGGGCGTACTTCGTCACGCAGCGGACGGACAAAGAATTCGTCAAAGGTGCGGTAGCTGGCg                                                            <  2:835679/80‑1 (MQ=255)                 ttCGGATCGGTATCGATTGGGCGTACTTCGTCACGCAGCGGACGGACAAAGAATTCGTCAAAGGTGCGGTAGCTGGCGgtg                                                         <  1:835681/81‑1 (MQ=255)                         ggTATCGATTGGGCGTACTTCGTCACGCAGCGGACGGACAAAGAATTCGTCAAAGGTGCGGTAGCTGGCGGTGTCCGGCtt                                                 >  1:835728/1‑81 (MQ=255)                         ggTATCGATTGGGCGTACTTCGTCACGCAGCGGACGGACAAAGAATTCGTCAAAGGTGCGGTAGCTGGCGGTGTCCGGCt                                                  <  1:835682/80‑1 (MQ=255)                          gTATCGATTGGGCGTACTTCGTCACGCAGCGGACGGACAAAGAATTCGTCAAAGGTGCGGTAGCTGGCGGTGTCCGGCttt                                                <  1:835684/81‑1 (MQ=255)                          gTATCGATTGGGCGTACTTCGTCACGCAGCGGACGGACAAAGAATTCGTCAAAGGTGCGGTAGCTGGCGGTGTCCGGCttt                                                <  2:835682/81‑1 (MQ=255)                           tATCGATTGGGCGTACTTCGTCACGCAGCGGGCGGACAAAGAATTCGTCAAAGGTGCGGTAGCTGGCGGTGTCCGGCtttt                                               <  2:835684/81‑1 (MQ=255)                                aTTGGGCGTACTTCGTCACGCAGCGGACGGACAAAGAATTCGTCAAAGGTGCGGTAGCTGGCGGTGTCCGGCTTTTGCGcc                                          >  1:835727/1‑81 (MQ=255)                                 ttGGGCGTACTTCGTCACGCAGCGGACGGACAAAGAATTCGTCAAAGGTGCGGTAGCTGGCGGTGTCCGGCTTTTGCGCct                                         <  2:835685/81‑1 (MQ=255)                                     gCGTACTTCGTCACGCAGCGGACGGACAAAGAATTCGTCAAAGGTGCGGTAGCTGGCGGTGTCCGGCTTTTGCGCCTCttt                                     <  2:835686/81‑1 (MQ=255)                                             cGTCACGCAGCGGACGGACAAAGAATTCGTCAAAGGTGCGGTAGCTGGCGGTGTCCGGCTTTTGCGCCTCTTTCATGTc                               <  1:835689/79‑1 (MQ=255)                                             cGTCACGCAGCGGACGGACAAAGAATTCGTCAAAGGTGCGGTAGCTGGCGGTGTCCGGCTTTTGCGCCTCTTTCATGTc                               <  2:835689/79‑1 (MQ=255)                                             cGTCACGCAGCGGACGGACAAAGAATTCGTCAAAGGTGCGGTAGCTGGCGGTGTCCGGCTTTTGCGCCTCTTTCATGTc                               <  1:835688/79‑1 (MQ=255)                                                 aCGCAGCGGACGGACAAAGAATTCGTCAAAGGTGCGGTAGCTGGCGGTGTCCGGCTTTTGCGCCTCTTTCATGTCGACCtt                         <  1:835691/81‑1 (MQ=255)                                                  cGCAGCGGACGGACAAAGAATTCGTCAAAGGTGCGGTAGCTGGCGGTGTCCGGCTTTTGCGCCTCTTTCATGTCGACCTTg                        >  2:835732/1‑81 (MQ=255)                                                   gCAGCGGACGGACAAAGAATTCGTCAAAGGTGCGGTAGCTGGCGGTGTCCGGCTTTTGCGCCTCTTTCATGTCGACCTTg                        >  1:835745/1‑80 (MQ=255)                                                        ggacggacAAAGAATTCGTCAAAGGTGCGGTAGCTGGCGGTGTCCGGCTTTTGCGCCTCTTTCATGTCGACCTTGTAGTAt                  <  2:835691/81‑1 (MQ=255)                                                             gacAAAGAATTCGTCAAAGGTGCGGTAGCTGGCGGTGTCCGGCTTTTGCGCCTCTTTCATGTCGACCTTGTAGTATTTAAc             >  1:835751/1‑81 (MQ=255)                                                                   gAATTCGTCAAAGGTGCGGTAGCTGGCGGTGTCCGGCTTTTGCGCCTCTTTCATGTCGACCTTGTAGTATTTAACGaa          >  2:835739/1‑78 (MQ=255)                                                                    aaTTCGTCAAAGGTGCGGTAGCTGGCGGTGTCCGGCTTTTGCGCCTCTTTCATGTCGACCTTGTAGTATTTAACGAACAGa      <  2:835692/81‑1 (MQ=255)                                                                        cGTCAAAGGTGCGGTAGCTGGCGGTGTCCGGCTTTTGCGCCTCTTTCATGTCGACCTTGTAGTATTTAACGAACAGATCGa  >  1:835735/1‑81 (MQ=255)                                                                           |                                                                              AGGCATGACCAGTACATTCGGATCGGTATCGATTGGGCGTACTTCGTCACGCAGCGGACGGACAAAGAATTCGTTAAAGGTGCGGTAGCTGGCGGTGTCCGGCTTTTGCGCCTCTTTCATGTCGACCTTGTAGTATTTAACGAACAGATCGA  >  NC_000913/4390097‑4390248

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑