Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	405,356	C→T	A97T (GCT→ACT)	proC ←	pyrroline‑5‑carboxylate reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	405,356	0	C	T	100.0%	85.7 / NA	25	A97T (GCT→ACT)	proC	pyrroline‑5‑carboxylate reductase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (17/8);  total (17/8)

TGTTCGGCATGGCGCGGATAATTTTCCGGTCATGGCCCAGCGCGCGGGCAAGCTGGTCGAGCGTGACACCTGCAGCAATAGAAACGACCAGAGAGTCTTTATTCAGGCTGGAGGTGATTTCGCTAAGCACTTTAATCATGATGCCAGGT  >  NC_000913/405281‑405429                                                                            |                                                                          tgtTCGGCATGGCGCGGATAATTTTCCGGTCATGGCCCAGCGCGCGGGCAAGCTGGTCGAGCGTGACACCTGCAGTaa                                                                         >  2:82750/1‑78 (MQ=255) tgtTCGGCATGGCGCGGATAATTTTCCGGTCATGGCCCAGCGCGCGGGCAAGCTGGTCGAGCGTGACACCTGCAGTAATAg                                                                      <  1:82726/81‑1 (MQ=255)  gtTCGGCATGGCGCGGATAATTTTCCGGTCATGGCCCAGCGCGCGGGCAAGCTGGTCGAGCGTGACACCTGCAGTAATAg                                                                      >  2:82774/1‑80 (MQ=255)         aTGGCGCGGATAATTTTCCGGTCATGGCCCAGCGCGCGGGCAAGCTGGTCGAGCGTGACACCTGCAGTAATAGAAACGAcc                                                              <  2:82727/81‑1 (MQ=255)               cgGATAATTTTCCGGTCATGGCCCAGCGCGCGGGCAAGCTGGTCGAGCGTGACACCTGCAGTAATAGAAACGACCagagag                                                        >  2:82771/1‑81 (MQ=255)                ggATAATTTTCCGGTCATGGCCCAGCGCGCGGGCAAGCTGGTCGAGCGTGACACCTGCAGTAATAGAAACGACCAGAGAGt                                                       >  2:82761/1‑81 (MQ=255)                     aTTTTCCGGTCATGGCCCAGCGCGCGGGCAAGCTGGTCGAGCGTGACACCTGCAGTAATAGAAACGACCAGAGAGTCttt                                                   <  1:82728/80‑1 (MQ=255)                       tttCCGGTCATGGCCCAGCGCGCGGGCAAGCTGGTCGAGCGTGACACCTGCAGTAATAGAAACGACCAGAGAGTCTTTAtt                                                >  1:82762/1‑81 (MQ=255)                           cGGTCATGGCCCAGCGCGCGGGCAAGCTGGTCGAGCGTGACACCTGCAGTAATAGAAACGACCAGAGAGTCTTTATTCAgg                                            >  2:82784/1‑81 (MQ=255)                           cGGTCATGGCCCAGCGCGCGGGCAAGCTGGTCGAGCGTGACACCTGCAGTAATAGAAACGACCAGAGAGTCTTTATTCAg                                             <  1:82729/80‑1 (MQ=255)                               cATGGCCCAGCGCGCGGGCAAGCTGGTCGAGCGTGACACCTGCAGTAATAGAAACGACCAGAGAGTCTTTATTCAGGCTg                                         >  2:82778/1‑80 (MQ=255)                                   gCCCAGCGCGCGGGCAAGCTGGTCGAGCGTGACACCTGCAGTAATAGAAACGACCAGAGAGTCTTTATTCAGGCTGGAGGt                                    <  2:82730/81‑1 (MQ=255)                                         cgcgcgGGCAAGCTGGTCGAGCGTGACACCTGCAGTAATAGAAACGACCAGAGAGTCTTTATTCAGGCTGGAGGTGATTTc                              >  2:82776/1‑81 (MQ=255)                                         cgcgcgGGCAAGCTGGTCGAGCGTGACACCTGCAGTAATAGAAACGACCAGAGAGTCTTTATTCAGGCTGGAGGTGATTTc                              >  2:82777/1‑81 (MQ=255)                                            gcgGGCAAGCTGGTCGAGCGTGACACCTGCAGTAATAGAAACGACCAGAGAGTCTTTATTCAGGCTGGAGGTGATTTCGc                            >  1:82764/1‑80 (MQ=255)                                                  aaGCTGGTCGAGCGTGACACCTGCAGTAATAGAAACGACCAGAGAGTCTTTATTCAGGCTGGAGGTGATTTCGCTAAGCAc                     >  2:82756/1‑81 (MQ=255)                                                  aaGCTGGTCGAGCGTGACACCTGCAGTAATAGAAACGACCAGAGAGTCTTTATTCAGGCTGGAGGTGATTTCGCTAAGCAc                     >  2:82775/1‑81 (MQ=255)                                                   aGCTGGTCGAGCGTGACACCTGCAGTAATAGAAACGACCAGAGAGTCTTTATTCAGGCTGGAGGTGATTTCGCTAAGCACt                    >  2:82779/1‑81 (MQ=255)                                                         tCGAGCGTGACACCTGCAGTAATAGAAACGACCAGAGAGTCTTTATTCAGGCTGGAGGTGATTTCGCTAAGCACTTTAATc              <  1:82731/81‑1 (MQ=255)                                                          cGAGCGTGACACCTGCAGTAATAGAAACGACCAGAGAGTCTTTATTCAGGCTGGAGGTGATTTCGCTAAGCACTTTAATCa             >  1:82769/1‑81 (MQ=255)                                                              cGTGACACCTGCAGTAATAGAAACGACCAGAGAGTCTTTATTCAGGCTGGAGGTGATTTCGCTAAGCACTTTAATCatgat         <  2:82732/81‑1 (MQ=255)                                                                tGACACCTGCAGTAATAGAAACGACCAGAGAGTCTTTATTCAGGCTGGAGGTGATTTCGCTAAGCACTTTAATCATGATGc       >  2:82773/1‑81 (MQ=255)                                                                   cacCTGCAGTAATGGAAACGACCAGAGAGTCTTTATTCAGGCTGGAGGTGATTTCGCTAAGCACTTTAATCATGATGCCAg    >  2:82767/1‑81 (MQ=255)                                                                    acCTGCAGTAATAGAAACGACCAGAGAGTCTTTATTCAGGCTGGAGGTGATTTCGCTAAGCACTTTAATCATGATGCCAgg   >  2:82770/1‑81 (MQ=255)                                                                     ccTGCAGTAATAGAAACGACCAGAGAGTCTTTATTCAGGCTGGAGGTGATTTCGCTAAGCACTTTAATCATGATGCCAGGt  <  1:82733/81‑1 (MQ=255)                                                                            |                                                                          TGTTCGGCATGGCGCGGATAATTTTCCGGTCATGGCCCAGCGCGCGGGCAAGCTGGTCGAGCGTGACACCTGCAGCAATAGAAACGACCAGAGAGTCTTTATTCAGGCTGGAGGTGATTTCGCTAAGCACTTTAATCATGATGCCAGGT  >  NC_000913/405281‑405429

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑