Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,419,570	T→C	E19G (GAG→GGG)	ulaG ←	L‑ascorbate‑6‑phosphate lactonase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,419,570	0	T	C	100.0%	69.5 / NA	21	E19G (GAG→GGG)	ulaG	L‑ascorbate‑6‑phosphate lactonase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (4/17);  total (4/17)

CACCACATCGCAAATGTGCCAGGAGCGACCTGTTCTTGTTCAATTTCTTCATTCAACCAGCTACCCCACTCCGGGAAAGTGCTCAGGATCCAGGATTCACGGGTGATACTTTTCACTTTACTCATCGCCATTTACCTTCAT  >  NC_000913/4419501‑4419641                                                                      |                                                                        caccacATCGCAAATGTGCCAGGAGCGACCTGTTCTTGTTCAATTTCTTCATTCAACCAGCTACCCCACCCCGGGAAAGTg                                                              <  2:843025/81‑1 (MQ=255)          gCAAATGTGCCAGGAGCGACCTGTTCTTGTTCAATTTCTTCATTCAACCAGCTACCCCACCCCGGGAAAGTGCTCAGGATc                                                     <  2:843026/81‑1 (MQ=255)            aaaTGTGCCAGGAGCGACCTGTTCTTGTTCAATTTCTTCATTCAACCAGCTACCCCACCCCGGGAAAGTGCTCAGGATCCa                                                   <  2:843027/81‑1 (MQ=255)               tgtgCCAGGAGCGACCTGTTCTTGTTCAATTTCTTCATTCAACCAGCTACCCCACCCCGGGAAAGTGCTCAGGATCCAGGa                                                >  2:843070/1‑81 (MQ=255)               tgtgCCAGGAGCGACCTGTTCTTGTTCAATTTCTTCATTCAACCAGCTACCCCACCCCGGGAAAGTGCTCAGGATCCAGGa                                                <  2:843028/81‑1 (MQ=255)                   ccAGGAGCGACCTGTTCTTGTTCAATTTCTTCATTCAACCAGCTACCCCACCCCGGGAAAGTGCTCAGGATCCAGGATTc                                             <  2:843029/80‑1 (MQ=255)                     aGGAGCGACCTGTTCTTGTTCAATTTCTTCATTCAACCAGCTACCCCACCCCGGGAAAGTGCTCAGGATCCAGGATTCACg                                          >  2:843069/1‑81 (MQ=255)                        aGCGACCTGTTCTTGTTCAATTTCTTCATTCAACCAGCTACCCCACCCCGGGAAAGTGCTCAGGATCCAGGATTCACGGGt                                       <  1:843031/81‑1 (MQ=255)                           gACCTGTTCTTGTTCAATTTCTTCATTCAACCAGCTACCCCACCCCGGGAAAGTGCTCAGGATCCAGGATTCACGGGTGAt                                    <  1:843032/81‑1 (MQ=255)                            aCCTGTTCTTGTTCAATTTCTTCATTCAACCAGCTACCCCACCCCGGGAAAGTGCTCAGGATCCAGGATTCACGGGTGATa                                   >  2:843066/1‑81 (MQ=255)                              cTGTTCTTGTTCAATTTCTTCATTCAACCAGCTACCCCACCCCGGGAAAGTGCTCAGGATCCAGGATTCACGGGTGATACt                                 <  2:843032/81‑1 (MQ=255)                                 ttCTTGTTCAATTTCTTCATTCAACCAGCTACCCCACCCCGGGAAAGTGCTCAGGATCCAGGATTCACGGGTGATACtt                                <  1:843034/79‑1 (MQ=255)                                      gTTCAATTTCTTCATTCAACCAGCTACCCCACCCCGGGAAAGTGCTCAGGATCCAGGATTCACGGGTGATACTTTTCAc                           <  2:843034/79‑1 (MQ=255)                                       ttCAATTTCTTCATTCAACCAGCTACCCCACCCCGGGAAAGTGCTCAGGATCCAGGATTCACGGGTGATACTTTTCACttt                        <  1:843036/81‑1 (MQ=255)                                              tcttcATTCAACCAGCTACCCCACCCCGGGAAAGTGCTCAGGATCCAGGATTCACGGGTGATACTTTTCACTTTACTCAt                  <  1:843037/80‑1 (MQ=255)                                                  cattcaACCAGCTACCCCACCCCGGGAAAGTGCTCAGGATCCAGGATTCACGGGTGATACTTTTCACTTTACTCATCGc               <  2:843037/79‑1 (MQ=255)                                                  cattcaACCAGCTACCCCACCCCGGGAAAGTGCTCAGGATCCAGGATTCACGGGTGATACTTTTCACTTTACTCATCGCCa             >  2:843076/1‑81 (MQ=255)                                                       aaCCAGCTACCCCACCCCGGGAAAGTGCTCAGGATCCAGGATTCACGGGTGATACTTTTCACTTTACTCATCGCCATTTAc        <  1:843040/81‑1 (MQ=255)                                                       aaCCAGCTACCCCACCCCGGGAAAGTGCTCAGGATCCAGGATTCACGGGTGATACTTTTCACTTTACTCATCGCCATTTAc        <  2:843038/81‑1 (MQ=255)                                                             cTACCCCACCCCGGGAAAGTGCTCAGGATCCAGGATTCACGGGTGATACTTTTCACTTTACTCATCGCCATTTACCTTCAt  <  1:843042/81‑1 (MQ=255)                                                             cTACCCCACCCCGGGAAAGTGCTCAGGATCCAGGATTCACGGGTGATACTTTTCACTTTACTCATCGCCATTTACCTTCAt  <  1:843041/81‑1 (MQ=255)                                                                      |                                                                        CACCACATCGCAAATGTGCCAGGAGCGACCTGTTCTTGTTCAATTTCTTCATTCAACCAGCTACCCCACTCCGGGAAAGTGCTCAGGATCCAGGATTCACGGGTGATACTTTTCACTTTACTCATCGCCATTTACCTTCAT  >  NC_000913/4419501‑4419641

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑