Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,425,249	C→T	R44R (CGC→CGT)	rpsF →	30S ribosomal subunit protein S6

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,425,249	0	C	T	100.0%	134.5 / NA	37	R44R (CGC→CGT)	rpsF	30S ribosomal subunit protein S6
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (23/14);  total (23/14)

GGCATGATCGAGCGCTACACTGCTGCCATCACTGGTGCAGAAGGCAAGATCCACCGTCTGGAAGACTGGGGCCGCCGTCAGCTGGCTTACCCGATCAACAAACTGCACAAAGCACACTACGTTCTGATGAATGTTGAAGCTCCGCAGGAAGT  >  NC_000913/4425175‑4425326                                                                           |                                                                              ggCATGATCGAGCGCTACACTGCTGCCATCACTGGTGCAGAAGGCAAGATCCACCGTCTGGAAGACTGGGGCCGTCGTCAg                                                                         >  2:844500/1‑81 (MQ=255) ggCATGATCGAGCGCTACACTGCTGCCATCACTGGTGCAGAAGGCAAGATCCACCGTCTGGAAGACTGGGGCCGTCGTCAg                                                                         >  1:844529/1‑81 (MQ=255)  gCATGATCGAGCGCTACACTGCTGCCATCACTGGTGCAGAAGGCAAGATCCACCGTCTGGAAGACTGGGGCCGTCGTCAGc                                                                        >  2:844503/1‑81 (MQ=255)       aTCGAGCGCTACACTGCTGCCATCACTGGTGCAGAAGGCAAGATCCACCGTCTGGAAGACTGGGGCCGTCGTCAGCTGGCt                                                                   >  1:844519/1‑81 (MQ=255)       aTCGAGCGCTACACTGCTGCCATCACTGGTGCAGAAGGCAAGATCCACCGTCTGGAAGACTGGGGCCGTCGTCAGCTGGCt                                                                   <  2:844468/81‑1 (MQ=255)            gcgcTACACTGCTGCCATCACTGGTGCAGAAGGCAAGATCCACCGTCTGGAAGACTGGGGCCGTCGTCAGCTGGCTTAccc                                                              >  2:844511/1‑81 (MQ=255)            gcgcTACACTGCTGCCATCACTGGTGCAGAAGGCAAGATCCACCGTCTGGAAGACTGGGGCCGTCGTCAGCTGGCTTAccc                                                              <  2:844470/81‑1 (MQ=255)            gcgcTACACTGCTGCCATCACTGGTGCAGAAGGCAAGATCCACCGTCTGGAAGACTGGGGCCGTCGTCAGCTGGCTTAccc                                                              <  2:844469/81‑1 (MQ=255)                 acacTGCTGCCATCACTGGTGCAGAAGGCAAGATCCACCGTCTGGAAGACTGGGGCCGTCGTCAGCTGGCTTACCCGATc                                                          >  2:844534/1‑80 (MQ=255)                 acacTGCTGCCATCACTGGTGCAGAAGGCAAGATCCACCGTCTGGAAGACTGGGGCCGTCGTCAGCTGGCTTACCCGATc                                                          >  1:844511/1‑80 (MQ=255)                  cacTGCTGCCATCACTGGTGCAGAAGGCAAGATCCACCGTCTGGAAGACTGGGGCCGTCGTCAGCTGGCTTACCCGATcaa                                                        >  1:844534/1‑81 (MQ=255)                  cacTGCTGCCATCACTGGTGCAGAAGGCAAGATCCACCGTCTGGAAGACTGGGGCCGTCGTCAGCTGGCTTACCCGATc                                                          <  1:844472/79‑1 (MQ=255)                    ctgctgCCATCACTGGTGCAGAAGGCAAGATCCACCGTCTGGAAGACTGGGGCCGTCGTCAGCTGGCTTACCCGATcaac                                                       >  1:844510/1‑80 (MQ=255)                      gctgcCATCACTGGTGCAGAAGGCAAGATCCACCGTCTGGAAGACTGGGGCCGTCGTCAGCTGGCTTACCCGATCAACaaa                                                    >  1:844525/1‑81 (MQ=255)                       ctgcCATCACTGGTGCAGAAGGCAAGATCCACCGTCTGGAAGACTGGGGCCGTCGTCAGCTGGCTTACCCGATcaacaa                                                     <  1:844473/79‑1 (MQ=255)                        tgcCATCACTGGTGCAGAAGGCAAGATCCACCGTCTGGAAGACTGGGGCCGTCGTCAGCTGGCTTACCCGATCAACAAACt                                                  >  1:844523/1‑81 (MQ=255)                               aCTGGTGCAGAAGGCAAGATCCACCGTCTGGAAGACTGGGGCCGTCGTCAGCTGGCTTACCCGATCAACAAACTGcaca                                             >  1:844518/1‑79 (MQ=255)                               aCTGGTGCAGAAGGCAAGATCCACCGTCTGGAAGACTGGGGCCGTCGTCAGCTGGCTTACCCGATCAACAAACTGcaca                                             >  2:844512/1‑79 (MQ=255)                                       aGAAGGCAAGATCCACCGTCTGGAAGACTGGGGCCGTCGTCAGCTGGCTTACCCGATCAACAAACTGCACAAAGCACACTa                                   <  1:844474/81‑1 (MQ=255)                                        gAAGGCAAGATCCACCGTCTGGAAGACTGGGGCCGTCGTCAGCTGGCTTACCCGATCAACAAACTGCACAAAGCACACTAc                                  >  2:844516/1‑81 (MQ=255)                                        gAAGGCAAGATCCACCGTCTGGAAGACTGGGGCCGTCGTCAGCTGGCTTACCCGATCAACAAACTGCACAAAGCACACTAc                                  <  2:844474/81‑1 (MQ=255)                                            gCAAGATCCACCGTCTGGAAGACTGGGGCCGTCGTCAGCTGGCTTACCCGATCAACAAACTGCACAAAGCACACTACGTTc                              >  1:844533/1‑81 (MQ=255)                                            gCAAGATCCACCGTCTGGAAGACTGGGGCCGTCGTCAGCTGGCTTACCCGATCAACAAACTGCACAAAGCACACTACGTTc                              <  1:844476/81‑1 (MQ=255)                                              aaGATCCACCGTCTGGAAGACTGGGGCCGTCGTCAGCTGGCTTACCCGATCAACAAACTGCACAAAGCACACTACGTTCt                             >  1:844527/1‑80 (MQ=255)                                              aaGATCCACCGTCTGGAAGACTGGGGCCGTCGTCAGCTGGCTTACCCGATCAACAAACTGCACAAAGCACACTACGTTCt                             >  1:844526/1‑80 (MQ=255)                                                 aTCCACCGTCTGGAAGACTGGGGCCGTCGTCAGCTGGCTTACCCGATCAACAAACTGCACAAAGCACACTACGTTCtgat                          >  1:844521/1‑80 (MQ=255)                                                  tCCACCGTCTGGAAGACTGGGGCCGTCGTCAGCTGGCTTACCCGATCAACAAACTGCACAAAGCACACTACGTTCtgatga                        <  2:844476/81‑1 (MQ=255)                                                    cACCGTCTGGAAGACTGGGGCCGTCGTCAGCTGGCTTACCCGATCAACAAACTGCACAAAGCACACTACGTTCTGATGAAt                      >  1:844537/1‑81 (MQ=255)                                                         tCTGGAAGACTGGGGCCGTCGTCAGCTGGCTTACCCGATCAACAAACTGCACAAAGCACACTACGTTCTGATGAATGTTg                  >  2:844537/1‑80 (MQ=255)                                                            ggAAGACTGGGGCCGTCGTCAGCTGGCTTACCCGATCAACAAACTGCACAAAGCACACTACGTTCTGATGAATGTTGAAGc              >  2:844535/1‑81 (MQ=255)                                                             gAAGACTGGGGCCGTCGTCAGCTGGCTTACCCGATCAACAAACTGCACAAAGCACACTACGTTCTGATGAATGTTGaa                <  2:844477/78‑1 (MQ=255)                                                              aaGACTGGGGCCGTCGTCAGCTGGCTTACCCGATCAACAAACTGCACAAAGCACACTACGTTCTGATGAATGTTGAAGCTc            <  2:844478/81‑1 (MQ=255)                                                                 aCTGGGGCCGTCGTCAGCTGGCTTACCCGATCAACAAACTGCACAAAGCACACTACGTTCTGATGAATGTTGAAGCTCCg          >  1:844540/1‑80 (MQ=255)                                                                 aCTGGGGCCGTCGTCAGCTGGCTTACCCGATCAACAAACTGCACAAAGCACACTACGTTCTGATGAATGTTGAAGCTCCg          >  2:844531/1‑80 (MQ=255)                                                                     gggCCGTCGTCAGCTGGCTTACCCGATCAACAAACTGCACAAAGCACACTACGTTCTGATGAATGTTGAAGCTCCGCAgg      <  2:844480/80‑1 (MQ=255)                                                                     gggCCGTCGTCAGCTGGCTTACCCGATCAACAAACTGCACAAAGCACACTACGTTCTGATGAATGTTGAAGCTCCGCAgg      <  1:844482/80‑1 (MQ=255)                                                                     gggCCGTCGTCAGCTGGCTTACCCGATCAACAAACTGCACAAAGCACACTACGTTCTGATGAATGTTGAAGCTCCGCAg       <  2:844479/79‑1 (MQ=255)                                                                        ccgtcgTCAGCTGGCTTACCCGATCAACAAACTGCACAAAGCACACTACGTTCTGATGAATGTTGAAGCTCCGCAGGAAGt  <  2:844483/81‑1 (MQ=255)                                                                        ccgtcgTCAGCTGGCTTACCCGATCAACAAACTGCACAAAGCACACTACGTTCTGATGAATGTTGAAGCTCCGCAGGAAGt  <  1:844483/81‑1 (MQ=255)                                                                           |                                                                              GGCATGATCGAGCGCTACACTGCTGCCATCACTGGTGCAGAAGGCAAGATCCACCGTCTGGAAGACTGGGGCCGCCGTCAGCTGGCTTACCCGATCAACAAACTGCACAAAGCACACTACGTTCTGATGAATGTTGAAGCTCCGCAGGAAGT  >  NC_000913/4425175‑4425326

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑