Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,429,686	T→C	intergenic (+131/‑178)	fklB → / → cycA	FKBP‑type peptidyl‑prolyl cis‑trans isomerase FklB/serine/alanine/glycine/cycloserine:H(+)symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,429,686	0	T	C	100.0%	96.8 / NA	28	intergenic (+131/‑178)	fklB/cycA	FKBP‑type peptidyl‑prolyl cis‑trans isomerase FklB/serine/alanine/glycine/cycloserine:H(+)symporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (12/16);  total (12/16)

ACCCTTCAGTTTGCCAAACGGCGCTATTTTGTGTTGCAAAGACCCCGTAAGCGTGTATTTTTGTGAGCTGTTTCGCGTTATCACCGTGATATGACACTCACTTTAAACATAAAATTAACATTAGATCTAAATCTTAGTATTCATCCCGCGTATTGT  >  NC_000913/4429608‑4429763                                                                               |                                                                              aCCCTTCAGTTTGCCAAACGGCGCTATTTTGTGTTGCAAAGACCCCGTAAGCGTGTATTTTTGTGAGCTGTTTCGCGTCAt                                                                             >  1:845492/1‑81 (MQ=255) aCCCTTCAGTTTGCCAAACGGCGCTATTTTGAGTTGCAAAGACCCCGTAAGCGTGTATTTTTGTGAGCTGTTTCGCGTCAt                                                                             <  2:845463/81‑1 (MQ=255)       cAGTTTGCCAAACGGCGCTATTTTGTGTTGCAAAGACCCCGTAAGCGTGTATTTTTGTGAGCTGTTTCGCGTCATCACCGt                                                                       >  1:845503/1‑81 (MQ=255)        aGTTTGCCAAACGGCGCTATTTTGTGTTGCAAAGACCCCGTAAGCGTGTATTTTTGTGAGCTGTTTCGCGTCATCACCGTg                                                                      <  2:845464/81‑1 (MQ=255)          tttGCCAAACGGCGCTATTTTGTGTTGCAAAGTCCCCGTAAGCGTGTATTTTTGTGAGCTGTTTCGCGTCATCACCGTGat                                                                    <  2:845465/81‑1 (MQ=255)           ttGCCAAACGGCGCTATTTTGTGTTGCAAAGACCCCGTAAGCGTGTATTTTTGTGAGCTGTTTCGCGTCATCACCGTGata                                                                   >  1:845506/1‑81 (MQ=255)               cAAACGGCGCTATTTTGTGTTGCAAAGACCCCGTAAGCGTGTATTTTTGTGAGCTGTTTCGCGTCATCACCGTGATATGa                                                                >  1:845498/1‑80 (MQ=255)                aaaCGGCGCTATTTTGTGTTGCAAAGACCCCGTAAGCGTGTATTTTTGTGAGCTGTTTCGCGTCATCACCGTGATATGaca                                                              >  1:845507/1‑81 (MQ=255)                    ggCGCTATTTTGTGTTGCAAAGACCCCGTAAGCGTGTATTTTTGTGAGCTGTTTCGCGTCATCACCGTGATATGAcactca                                                          <  2:845466/81‑1 (MQ=255)                      cgcTATTTTGTGTTGCAAAGACCCCGTAAGCGTGTATTTTTGTGAGCTGTTTCGCGTCATCACCGTGATATGAcactcact                                                        <  2:845468/81‑1 (MQ=255)                      cgcTATTTTGTGTTGCAAAGACCCCGTAAGCATGTATTTTTGTGAGCTGTTTCGCGTCATCACCGTGATATGAcactcact                                                        <  2:845467/81‑1 (MQ=255)                       gcTATTTTGTGTTGCAAAGACCCCGTAAGCGTGTATTTTTGTGAGCTGTTTCGCGTCATCACCGTGATATGACACTCACtt                                                       <  1:845470/81‑1 (MQ=255)                           ttttGTGTTGCAAAGACCCCGTAAGCGTGTATTTTTGTGAGCTGTTTCGCGTCATCACCGTGATATGACACTCACTTTa                                                     <  1:845471/79‑1 (MQ=255)                             ttGTGTTGCAAAGACCCCGTAAGCGTGTATTTTTGTGAGCTGTTTCGCGTCATCACCGTGATATGACACTCACTTTAAACa                                                 >  1:845516/1‑81 (MQ=255)                              tgtgTTGCAAAGACCCCGTAAGCGTGTATTTTTGTGAGCTGTTTCGCGTCATCACCGTGATATGACACTCACTTTAAACa                                                 >  2:845511/1‑80 (MQ=255)                                   tGCAAAGACCCCGTAAGCGTGTATTTTTGTGAGCTGTTTCGCGTCATCACCGTGATATGACACTCACTTTAAACATAAAAt                                           >  1:845499/1‑81 (MQ=255)                                         gACCCCGTAAGCGTGTATTTTTGTGAGCTGTTTCGCGTCATCACCGTGATATGACACTCACTTTAAACATAAAATTAACAt                                     >  2:845503/1‑81 (MQ=255)                                           ccccGTAAGCGTGTATTTTTGTGAGCTGTTTCGCGTCATCACCGTGATATGACACTCACTTTAAACATAAAATTAACATTa                                   <  2:845471/81‑1 (MQ=255)                                               gTAAGCGTGTATTTTTGTGAGCTGTTTCGCGTCATCACCGTGATATGACACTCACTTTAAACATAAAATTAACATTAGATc                               >  1:845515/1‑81 (MQ=255)                                                           ttttGTGAGCTGTTTCGCGTCATCACCGTGATATGACACTCACTTTAAACATAAAATTAACATTAGATCTAAATCTTAGTa                   >  2:845509/1‑81 (MQ=255)                                                              tgtgAGCTGTTTCGCGTCATCACCGTGATATGACACTCACTTTAAACATAAAATTAACATTAGATCTAAATCTTAGTAtt                 <  1:845473/80‑1 (MQ=255)                                                                tgAGCTGTTTCGCGTCATCACCGTGATATGACACTCACTTTAAACATAAAATTAACATTAGATCTAAATCTTAGTATTCAt              <  1:845474/81‑1 (MQ=255)                                                                    cTGTTTCGCGTCATCACCGTGATATGACACTCACTTTAAACATAAAATTAACATTAGATCTAAATCTTAGTATTCATCCcg          <  2:845474/81‑1 (MQ=255)                                                                       tttCGCGTCATCACCGTGATATGACACTCACTTTAAACATAAAATTAACATTAGATCTAAATCTTAGTATTCATCCCGCGt       <  1:845477/81‑1 (MQ=255)                                                                       tttCGCGTCATCACCGTGATATGACACTCACTTTAAACATAAAATTAACATTAGATCTAAATCTTAGTATTCATCCCGCGt       <  2:845475/81‑1 (MQ=255)                                                                       tttCGCGTCATCACCGTGATATGACACTCACTTTAAACATAAAATTAACATTAGATCTAAATCTTAGTATTCATCCCGCGt       <  2:845477/81‑1 (MQ=255)                                                                           gcgTCACCACCGTGATATGACACTCACTTTAAACATAAAATTAACATTAGATCTAAATCTTAGTATTCATCCCGCGTAtt    <  2:845478/80‑1 (MQ=255)                                                                            cgTCATCACCGTGATATGACACTCACTTTAAACATAAAATTAACATTAGATCTAAATCTTAGTATTCATCCCGCGTATTGt  >  2:845513/1‑81 (MQ=255)                                                                               |                                                                              ACCCTTCAGTTTGCCAAACGGCGCTATTTTGTGTTGCAAAGACCCCGTAAGCGTGTATTTTTGTGAGCTGTTTCGCGTTATCACCGTGATATGACACTCACTTTAAACATAAAATTAACATTAGATCTAAATCTTAGTATTCATCCCGCGTATTGT  >  NC_000913/4429608‑4429763

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑