Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,453,747	G→A	V55V (GTG→GTA)	yjfF →	galactofuranose ABC transporter putative membrane subunit YjtF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,453,747	0	G	A	100.0%	146.4 / NA	40	V55V (GTG→GTA)	yjfF	galactofuranose ABC transporter putative membrane subunit YjtF
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (24/16);  total (24/16)

TGCTTCCACAAGAGTGATCTGCAATATCCTGACCGATAATGCCTTTCTTGGGATCATTGCCGTTGGCATGACCTTTGTGATCCTCTCAGGTGGGATCGATCTCTCCGTCGGTTCGGTGATCGCCTTTACTGGCGTGTTTCTGGCAA  >  NC_000913/4453669‑4453814                                                                               |                                                                    tGCTTCCACAAGAGTGATCTGCAATATCCTGACCGATAATGCCTTTCTTGGGATCATTGCCGTTGGCATGACCTTTGTAAt                                                                   >  1:851271/1‑81 (MQ=255)  gCTTCCACAAGAGTGATCTGCAATATCCTGACCGATAATGCCTTTCTTGGGATCATTGCCGTTGGCATGACCTTTGTAAt                                                                   <  2:851217/80‑1 (MQ=255)    ttCCACAAGAGTGATCTGCAATATCCTGACCGATAATGCCTTTCTTGGGATCATTGCCGTTGGCATGACCTTTGTAATCct                                                                >  1:851260/1‑81 (MQ=255)      ccACAAGAGTGATCTGCAATATCCTGACCGATAATGCCTTTCTTGGGATCATTGCCGTTGGCATGACCTTTGTAATCctct                                                              >  2:851275/1‑81 (MQ=255)      ccACAAGAGTGATCTGCAATATCCTGACCGATAATGCCTTTCTTGGGATCATTGCCGTTGGCATGACCTTTGTAATCctct                                                              >  2:851289/1‑81 (MQ=255)         caAGAGTGATCTGCAATATCCTGACCGATAATGCCTTTCTTGGGATCATTGCCGTTGGCATGACCTTTGTAATCCTCTCAg                                                           >  2:851279/1‑81 (MQ=255)         caAGAGTGATCTGCAATATCCTGACCGATAATGCCTTTCTTGGGATCATTGCCGTTGGCATGACCTTTGTAATCCTCTCAg                                                           >  1:851275/1‑81 (MQ=255)            gagTGATCTGCAATATCCTGACCGATAATGCCTTTCTTGGGATCATTGCCGTTGGCATGACCTTTGTAATCCTCTCAGGTg                                                        >  2:851283/1‑81 (MQ=255)            gagTGATCTGCAATATCCTGACCGATAATGCCTTTCTTGGGATCATTGCCGTTGGCATGACCTTTGTAATCCTCTCAGGTg                                                        >  2:851293/1‑81 (MQ=255)                gATCTGCAATATCCTGACCGATAATGCCTTTCTTGGGATCATTGCCGTTGGCATGACCTTTGTAATCCTCTCAGGTGGgat                                                    >  2:851277/1‑81 (MQ=255)                gATCTGCAATATCCTGACCGATAATGCCTTTCTTGGGATCATTGCCGTTGGCATGACCTTTGTAATCCTCTCAGGTGGgat                                                    >  2:851267/1‑81 (MQ=255)                 aTCTGCAATATCCTGACCGATAATGCCTTTCTTGGGATCATTGCCGTTGGCATGACCTTTGTAATCCTCTCAGGTGGgat                                                    <  1:851219/80‑1 (MQ=255)                  tCTGCAATATCCTGACCGATAATGCCTTTCTTGGGATCATTGCCGTTGGCATGACCTTTGTAATCCTCTCAGGTGGgatc                                                   >  1:851266/1‑80 (MQ=255)                     gCAATATCCTGACCGATAATGCCTTTCTTGGGATCATTGCCGTTGGCATGACCTTTGTAATCCTCTCAGGTGGgatcgatc                                               >  1:851220/1‑81 (MQ=255)                      cAATATCCTGACCGATAATGCCTTTCTTGGGATCATTGCCGTTGGCATGACCTTTGTAATCCTCTCAGGTGGGATCGAtct                                              <  1:851221/81‑1 (MQ=255)                      cAATATCCTGACCGATAATGCCTTTCTTGGGATCATTGCCGTTGGCATGACCTTTGTAATCCTCTCAGGTGGGATCGAtct                                              >  2:851263/1‑81 (MQ=255)                       aaTATCCTGACCGATAATGCCTTTCTTGGGATCATTGCCGTTGGCATGACCTTTGTAATCCTCTCAGGTGGGATCGAtctc                                             <  1:851222/81‑1 (MQ=255)                             cTGACCGATAATGCCTTTCTTGGGATCATTGCCGTTGGCATGACCTTTGTAATCCTCTCAGGTGGGATCGATCTCTCCGt                                        <  1:851223/80‑1 (MQ=255)                               gACCGATAATGCCTTTCTTGGGATCATTGCCGTTGGCATGACCTTTGTAATCCTCTCAGGTGGGATCGATCTCTCCGtcgg                                     >  1:851270/1‑81 (MQ=255)                               gACCGATAATGCCTTTCTTGGGATCATTGCCGTTGGCATGACCTTTGTAATCCTCTCAGGTGGGATCGATCTCTCCGtcgg                                     >  1:851289/1‑81 (MQ=255)                                aCCGATAATGCCTTTCTTGGGATCATTGCCGTTGGCATGACCTTTGTAATCCTCTCAGGTGGGATCGATCTCTCCGtcgg                                     <  2:851223/80‑1 (MQ=255)                                 ccGATAATGCCTTTCTTGGGATCATTGCCGTTGGCATGACCTTTGTAATCCTCTCAGGTGGGATCGATCTCTCCGtcggtt                                   <  1:851226/81‑1 (MQ=255)                                 ccGATAATGCCTTTCTTGGGATCATTGCCGTTGGCATGACCTTTGTAATCCTCTCAGGTGGGATCGATCTCTCCGtcggtt                                   <  2:851224/81‑1 (MQ=255)                                  cGATAATGCCTTTCTTGGGATCATTGCCGTTGGCATGACCTTTGTAATCCTCTCAGGTGGGATCGATCTCTCCGtcggttc                                  <  1:851227/81‑1 (MQ=255)                                  cGATAATGCCTTTCTTGGGATCATTGCCGTTGGCATGACCTTTGTAATCCTCTCAGGTGGGATCGATCTCTCCGtcggttc                                  >  2:851272/1‑81 (MQ=255)                                   gATAATGCCTTTCTTGGGATCATTGCCGTTGGCATGACCTTTGTAATCCTCTCAGGTGGGATCGATCTCTCCGtcggttcg                                 <  2:851227/81‑1 (MQ=255)                                      aaTGCCTTTCTTGGGATCATTGCCGTTGGCATGACCTTTGTAATCCTCTCAGGTGGGATCGATCTCTCCGtcggttcggt                               >  1:851261/1‑80 (MQ=255)                                              tCTTGGGATCATTGCCGTTGGCATGACCTTTGTAATCCTCTCAGGTGGGATCGATCTCTCCGTCGGTTCGGTGATCGCCtt                      >  1:851309/1‑81 (MQ=255)                                                   ggATCATTGCCGTTGGCATGACCTTTGTAATCCTCTCAGGTGGGATCGATCTCTCCGTCGGTTCGGTGATCGCCTTTACTg                 <  1:851229/81‑1 (MQ=255)                                                   ggATCATTGCCGTTGGCATGACCTTTGTAATCCTCTCAGGTGGGATCGATCTCTCCGTCGGTTCGGTGATCGCCTTTACTg                 <  1:851230/81‑1 (MQ=255)                                                    gATCATTGCCGTTGGCATGACCTTTGTAATCCTCTCAGGTGGGATCGATCTCTCCGTCGGTTCGGTGATCGCCTTTACTgg                >  1:851269/1‑81 (MQ=255)                                                     aTCATTGCCGTTGGCATGACCTTTGTAATCCTCTCAGGTGGGATCGATCTCTCCGTCGGTTCGGTGATCGCCTTTACTGGc               >  2:851287/1‑81 (MQ=255)                                                         ttGCCGTTGGCATGACCTTTGTAATCCTCTCAGGTGGGATCGATCTCTCCGTCGGTTCGGTGATCGCCTTTACTGGCgtgt           >  2:851302/1‑81 (MQ=255)                                                           gCCGTTGGCATGACCTTTGTAATCCTCTCAGGTGGGATCGATCTCTCCGTCGGTTCGGTGATCGCCTTTACTGGCGTGtt          <  2:851230/80‑1 (MQ=255)                                                           gCCGTTGGCATGACCTTTGTAATCCTCTCAGGTGGGATCGATCTCTCCGTCGGTTCGGTGATCGCCTTTACTGGCGTGtt          <  1:851232/80‑1 (MQ=255)                                                            ccGTTGGCATGACCTTTGTAATCCTCTCAGGTGGGATCGATCTCTCCGTCGGTTCGGTGATCGCCTTTACTGGCGTGTTTc        >  1:851306/1‑81 (MQ=255)                                                            ccGTTGGCATGACCTTTGTAATCCTCTCAGGTGGGATCGATCTCTCCGTCGGTTCGGTGATCGCCTTTACTGGCGTGTTTc        <  1:851233/81‑1 (MQ=255)                                                               ttGGCATGACCTTTGTAATCCTCTCAGGTGGGATCGATCTCTCCGTCGGTTCGGTGATCGCCTTTACTGGCGTGTTTCTgg     <  1:851234/81‑1 (MQ=255)                                                                tGGCATGACCTTTGTAATCCTCTCAGGTGGGATCGATCTCTCCGTCGGTTCGGTGATCGCCTTTACTGGCGTGTTTCTgg     >  2:851290/1‑80 (MQ=255)                                                                   cATGACCTTTGTAATCCTCTCAGGTGGGATCGATCTCTCCGTCGGTTCGGTGATCGCCTTTACTGGCGTGTTTCTGGCaa  >  2:851286/1‑80 (MQ=255)                                                                               |                                                                    TGCTTCCACAAGAGTGATCTGCAATATCCTGACCGATAATGCCTTTCTTGGGATCATTGCCGTTGGCATGACCTTTGTGATCCTCTCAGGTGGGATCGATCTCTCCGTCGGTTCGGTGATCGCCTTTACTGGCGTGTTTCTGGCAA  >  NC_000913/4453669‑4453814

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑