Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,477,066	G→A	V544I (GTT→ATT)	yjgL →	protein YjgL

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,477,066	0	G	A	100.0%	58.1 / NA	17	V544I (GTT→ATT)	yjgL	protein YjgL
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (13/4);  total (13/4)

ATAATTTAAAATATGATTTAAATGATAATGATAAGGAATCTAATAAAGAAATTTTGAAGAATCAACCAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAG  >  NC_000913/4476992‑4477138                                                                           |                                                                         aTAATTTAAAATATGATTTAAATGATAATGATAAGGAATCTAATAAAGAAATTTTGAAGAATCAACCAGATAATATTATCa                                                                    <  1:856700/81‑1 (MQ=255)  tAATTTAAAATATGATTTAAATGATAATGATAAGGAATCTAATAAAGAAATTTCGAAGAATCAACCAGATAATATTATCa                                                                    >  2:856726/1‑80 (MQ=255)        aaaaTATGATTTAAATGATAATGATAAGGAATCTAATAAAGAAATTTTGAAGAATCAACCAGATAATATTATCAAAGaaa                                                              >  1:856714/1‑80 (MQ=255)        aaaaTATGATTTAAATGATAATGATAAGGAATCTAATAAAGAAATTGTGAAGAATCAACCAGATAATATTATCAAAGaaa                                                              <  2:856700/80‑1 (MQ=255)          aaTATGATTTAAATGATAATGATAAGGAATCTAATAAAGAAATTTTGAAGAATCAACCAGATAATATTATCAAAGAAAAAc                                                           >  1:856712/1‑81 (MQ=255)                       tgataatgataaGGAATCTAATAAAGAAATTTTGAAGAATCAACCAGATAATATTATCAAAGAAAAACTGAATAATGAATa                                              >  1:856736/1‑81 (MQ=255)                               ataaGGAATCTAATAAAGAAATTTTGAAGAATCAACCAGATAATATTATCAAAGAAAAACTGAATAATGAATACAAACt                                        >  1:856717/1‑79 (MQ=255)                                 aaGGAATCTAATAAAGAAATTTTGAAGAATCAACCAGATAATATTATCAAAGAAAAACTGAATAATGAATACAAACt                                        >  1:856730/1‑77 (MQ=255)                                  aGGAATCTAATAAAGAAATTTTGAAGAATCAACCAGATAATATTATCAAAGAAAAACTGAATAATGAATACAAACTTAGa                                    <  1:856702/80‑1 (MQ=255)                                    gAATCTAATAAAGAAATTTTGAAGAATCAACCAGATAATATTATCAAAGAAAAACTGAATAATGAATACAAACTTAGAtt                                  >  1:856720/1‑80 (MQ=255)                                     aaTCTAATAAAGAAATTTTGAAGAATCAACCAGATAATATTATCAAAGAAAAACTGAATAATGAATACAAACTTAGAtt                                  >  1:856719/1‑79 (MQ=255)                                     aaTCTAATAAAGAAATTTTGAAGAATCAACCAGATAATATTATCAAAGAAAAACTGAATAATGAATACAAACTTAGAtt                                  >  1:856715/1‑79 (MQ=255)                                                gAAATTTTGAAGAATCAACCAGATAATATTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCa                      >  2:856720/1‑80 (MQ=255)                                                         aagaaTCAACCAGATAATATTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTg            >  2:856721/1‑81 (MQ=255)                                                           gaaTCAACCAGATAATATTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGc           >  2:856732/1‑80 (MQ=255)                                                                aaCCAGATAATATTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATc       >  1:856738/1‑79 (MQ=255)                                                                   cAGATAATATTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGagag  <  2:856702/81‑1 (MQ=255)                                                                           |                                                                         ATAATTTAAAATATGATTTAAATGATAATGATAAGGAATCTAATAAAGAAATTTTGAAGAATCAACCAGATAATGTTATCAAAGAAAAACTGAATAATGAATACAAACTTAGATTTAGAATGATGCAAACTATCTTGCAATCGAGAG  >  NC_000913/4476992‑4477138

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑