Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,502,703	G→A	I243I (ATC→ATT)	insG ←	KpLE2 phage‑like element; IS4 putative transposase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,502,703	0	G	A	100.0%	110.4 / NA	32	I243I (ATC→ATT)	insG	KpLE2 phage‑like element; IS4 putative transposase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (15/17);  total (15/17)

CAGTCCCGGCCACTTTTTTCGTGCCTGCGGGCTGGTTTTCAGCTTCACCAGATGATCGCCTTTACCCAGTTTTCTGATCTCTTCATATTGCGCTCCCTTTCTGAGAGGTATCATCCAGTGGCGGTGTTCTCCCGCCAGGCTCCAGGCATT  >  NC_000913/4502628‑4502777                                                                            |                                                                           cAGTCCCGGCCACTTTTTTCGTGCCTGCGGGCTGGTTTTCAGCTTCACCAGATGATCGCCTTTACCCAGTTTTCTAAtctc                                                                       <  2:862458/81‑1 (MQ=255)  aGTCCCGGCCACTTTTTTCGTGCCTGCGGGCTGGTTTTCAGCTTCACCAGATGATCGCCTTTACCCAGTTTTCTAATctct                                                                      >  2:862494/1‑81 (MQ=255)    tCCCGGCCACTTTTTTCGTGCCTGCGGGCTGGTTTTCAGCTTCACCAGATGATCGCCTTTACCCAGTTTTCTAATCTCTTc                                                                    <  1:862460/81‑1 (MQ=255)     cccGGCCACTTTTTTCGTGCCTGCGGGCTGGTTTTCAGCTTCACCAGATGATCGCCTTTACCCAGTTTTCTAATCTCTTCa                                                                   >  1:862512/1‑81 (MQ=255)      ccGGCCACTTTTTTCGTGCCTGCGGGCTGGTTTTCAGCTTCACCAGATGATCGCCTTTACCCAGTTTTCTAATCTCTTCa                                                                   >  2:862516/1‑80 (MQ=255)       cGGCCACTTTTTTCGTGCCTGCGGGCTGGTTTTCAGCTTCACCAGATGATCGCCTTTACCCAGTTTTCTAATCTCTTCata                                                                 <  2:862460/81‑1 (MQ=255)        ggCCACTTTTTTCGTGCCTGCGGGCTGGTTTTCAGCTTCACCAGATGATCGCCTTTACCCAGTTTTCTAATCTCTTCatct                                                                <  1:862463/81‑3 (MQ=255)        ggCCACTTTTTTCGTGCCTGCGGGCTGGTTTTCAGCTTCACCAGATGATCGCCTTTACCCAGTTTTCTAATCTCTTCatat                                                                >  2:862502/1‑81 (MQ=255)        ggCCACTTTTTTCGTGCCTGCGGGCTGGTTTTCAGCTTCACCAGATGATCGCCTTTACCCAGTTTTCTAATCTCTTCatat                                                                <  1:862462/81‑1 (MQ=255)            aCTTTTTTCGTGCCTGCGGGCTGGTTTTCAGCTTCACCAGATGATCGCCTTTACCCAGTTTTCTAATCTCTTCATATTgcg                                                            >  1:862508/1‑81 (MQ=255)                  ttCGTGCCTGCGGGCTGGTTTTCAGCTTCACCAGATGATCGCCTTTACCCAGTTTTCTAATCTCTTCATATTGCGCTccc                                                       <  1:862464/80‑1 (MQ=255)                   tCGTGCCTGCGGGCTGGTTTTCAGCTTCACCAGATGATCGCCTTTACCCAGTTTTCTAATCTCTTCATATTGCGCTCCCt                                                      <  2:862464/80‑1 (MQ=255)                     gTGCCTGCGGGCTGGTTTTCAGCTTCACCAGATGATCGCCTTTACCCAGTTTTCTAATCTCTTCATATTGCGCTCCCTTTc                                                   >  1:862505/1‑81 (MQ=255)                        ccTGCGGGCTGGTTTTCAGCTTCACCAGATGATCGCCTTTACCCAGTTTTCTAATCTCTTCATATTGCGCTCCCTTTCTg                                                 <  2:862465/80‑1 (MQ=255)                          tGCGGGCTGGTTTTCAGCTTCACCAGATGATCGCCTTTACCCAGTTTTCTAATCTCTTCATATTGCGCTCCCTTTCTga                                                >  2:862518/1‑79 (MQ=255)                          tGCGGGCTGGTTTTCAGCTTCACCAGATGATCGCCTTTACCCAGTTTTCTAATCTCTTCATATTGCGCTCCCTTTCTga                                                >  1:862510/1‑79 (MQ=255)                          tGCGGGCTGGTTTTCAGCTTCACCAGATGATCGCCTTTACCCAGTTTTCCAATCTCTTCATATTGCGCTCCCTTTCTga                                                >  2:862528/1‑79 (MQ=255)                              ggCTGGTTTTCAGCTTCACCAGATGATCGCCTTTACCCAGTTTTCTAATCTCTTCATATTGCGCTCCCTTTCTGAGAGGTa                                          >  1:862526/1‑81 (MQ=255)                                   gTTTTCAGCTTCACCAGATGATCGCCTTTACCCAGTTTTCTAATCTCTTCATATTGCGCTCCCTTTCTGAGAGGTatcatc                                     >  1:862524/1‑81 (MQ=255)                                   gTTTTCAGCTTCACCAGATGATCGCCTTTACCCAGTTTTCTAATCTCTTCATATTGCGCTCCCTTTCTGAGAGGTatcat                                      <  1:862467/80‑1 (MQ=255)                                      ttCAGCTTCACCAGATGATCGCCTTTACCCAGTTTTCTAATCTCTTCATATTGCGCTCCCTTTCTGAGAGGTATCATCCAg                                  >  2:862515/1‑81 (MQ=255)                                        cAGCTTCACCAGATGATCGCCTTTACCCAGTTTTCTAATCTCTTCATATTGCGCTCCCTTTCTGAGAGGTATCATCCAGt                                 <  1:862468/80‑1 (MQ=255)                                          gCTTCACCAGATGATCGCCTTTACCCAGTTTTCTAATCTCTTCATATTGCGCTCCCTTTCTGAGAGGTATCATCCAGTGGc                              <  2:862468/81‑1 (MQ=255)                                           cTTCACCAGATGATCGCCTTTACCCAGTTTTCTAATCTCTTCATATTGCGCTCCCTTTCTGAGAGGTATCATCCAGTGGCg                             <  2:862469/81‑1 (MQ=255)                                                    atgatCGCCTTTACCCAGTTTTCTAATCTCTTCATATTGCGCTCCCTTTCTGAGAGGTATCATCCAGTGGCGGTGTTCTcc                    >  1:862531/1‑81 (MQ=255)                                                     tgatCGCCTTTACCCAGTTTTCTAATCTCTTCATATTGCGCTCCCTTTCTGAGAGGTATCATCCAGTGGCGGTGTTCTccc                   <  1:862471/81‑1 (MQ=255)                                                          gCCTTTACCCAGTTTTCTAATCTCTTCATATTGCGCTCCCTTTCTGAGAGGTATCATCCAGTGGCGGTGTTCTCCCGCCa               <  1:862472/80‑1 (MQ=255)                                                              ttACCCAGTTTTCTAATCTCTTCATATTGCGCTCCCTTTCTGAGAGGTATCATCCAGTGGCGGTGTTCTCCCGCCAGGCTc          >  1:862518/1‑81 (MQ=255)                                                                 cccAGTTTTCTAATCTCTTCATATTGCGCTCCCTTTCTGAGAGGTATCATCCAGTGGCGGTGTTCTCCCGCCAGGCTCCa        <  2:862473/80‑1 (MQ=255)                                                                 cccAGTTTTCTAATCTCTTCATATTGCGCTCCCTTTCTGAGAGGTATCATCCAGTGGCGGTGTTCTCCCGCCAGGCTCCAg       <  1:862473/81‑1 (MQ=255)                                                                   cAGTTTTCTAATCTCTTCATATTGCGCTCCCTTTCTGAGAGGTATCATCCAGTGGCGGTGTTCTCCCGCCAGGCTCCAGGc     <  1:862475/81‑1 (MQ=255)                                                                      ttttCTAATCTCTTCATATTGCGCTCCCTTTCTGAGAGGTATCATCCAGTGGCGGTGTTCTCCCGCCAGGCTCCAGGCAtt  >  2:862522/1‑81 (MQ=255)                                                                            |                                                                           CAGTCCCGGCCACTTTTTTCGTGCCTGCGGGCTGGTTTTCAGCTTCACCAGATGATCGCCTTTACCCAGTTTTCTGATCTCTTCATATTGCGCTCCCTTTCTGAGAGGTATCATCCAGTGGCGGTGTTCTCCCGCCAGGCTCCAGGCATT  >  NC_000913/4502628‑4502777

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑