Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,504,741	A→G	L228L (CTA→CTG)	yjhB →	putative sialic acid transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,504,741	0	A	G	100.0%	60.1 / NA	18	L228L (CTA→CTG)	yjhB	putative sialic acid transporter
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (7/11);  total (7/11)

AAATCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGT  >  NC_000913/4504664‑4504809                                                                              |                                                                     aaaTCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTGTCTGttt                                                                   >  2:862918/1‑81 (MQ=255)  aaTCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTGTCTGTTTg                                                                  <  1:862884/81‑1 (MQ=255)   aTCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTGTCTGTTTgg                                                                 <  1:862885/81‑1 (MQ=255)      aaCATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTGTCTGTTTGGTg                                                               <  1:862886/80‑1 (MQ=255)       aCATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTGTCTGTTTGGTGc                                                              >  1:862913/1‑80 (MQ=255)       aCATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTGTCTGTTTGGTGc                                                              >  1:862918/1‑80 (MQ=255)             ttGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTGTCTGTTTGGTGCAAACTgg                                                       >  1:862916/1‑81 (MQ=255)                tctgtctTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTGTCTGTTTGGTGCAAACTGGcc                                                     <  2:862886/80‑1 (MQ=255)                           aaaaCCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTGTCTGTTTGGTGCAAACTGGCCGATAAACGGac                                          >  1:862902/1‑80 (MQ=255)                               ccACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTGTCCGTTTGGTGCAAACTGGCCGATAAACGGACTActt                                     <  1:862888/81‑1 (MQ=255)                                        tCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTGTCTGTTTGGTGCAAACTGGCCGATAAACGGACTActtccttc                                <  2:862890/77‑1 (MQ=255)                                        tCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTGTCTGTTTGGTGCAAACTGGCCGATAAACGGACTACttccttcc                               <  2:862888/78‑1 (MQ=255)                                        tCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTGTCTGTTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTa                             <  1:862890/80‑1 (MQ=255)                                        tCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTGTCTGTTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTAc                            >  1:862917/1‑81 (MQ=255)                                         cAATCTCTATGATCGTTTTCCTCGTCTGTTTTTGTCTGTTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTAcc                           >  1:862921/1‑81 (MQ=255)                                           aTCTCTATGATCGTTTTCCTCGTCTGTTTTTGTCTGTTTGGTGCAAACTGGCCGATAAACAGACTACTTCCTTCCTACCTg                         <  2:862891/81‑1 (MQ=255)                                                             cTCGTCTGTTTTTGTCTGTTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTACCTGGCAGATAATGGAGTTAAt       <  1:862893/81‑1 (MQ=255)                                                                     tttttGTCTGTTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGt  <  1:862894/78‑1 (MQ=255)                                                                              |                                                                     AAATCAACATTTTTGTCTGTCTTCAGAAAACCACATCTTTCAATCTCTATGATCGTTTTCCTCGTCTGTTTTTGTCTATTTGGTGCAAACTGGCCGATAAACGGACTACTTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGT  >  NC_000913/4504664‑4504809

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑