Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,507,390	T→C	pseudogene (194/255 nt)	insO →	KpLE2 phage‑like element; IS911B regulator fragment

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,507,390	0	T	C	100.0%	76.8 / NA	23	pseudogene (194/255 nt)	insO	KpLE2 phage‑like element; IS911B regulator fragment
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (9/14);  total (9/14)

CAAGATGGGTCAAACAACTGCGTGATGAGCGTCAGGGCAAAACACCAAAAGCCTCTCCGATAACACCAGAACAAATCGAAATACGTAAGCTGAGGAAAAAGCTACAACGGATTGAAATGGAGAATGAAATATTAAAAAGGCTACTGTAGAT  >  NC_000913/4507315‑4507465                                                                            |                                                                            cAAGATGGGTCAAACAACTGCGTGATGAGCGTCAGGGCAAAACACCAAAAGCCTCTCCGATAACACCAGAACAAACCGaaa                                                                        >  1:863325/1‑81 (MQ=255)  aaGATGGGTCAAACAACTGCGTGATGAGCGTCAGGGCAAAACACCAAAAGCCTCTCCGATAACACCAGAACAAACCGAAAt                                                                       <  1:863293/81‑1 (MQ=255)       gggTCAAACAACTGCGTGATGAGCGTCAGGGCAAAACACCAAAAGCCTCTCCGATAACACCAGAACAAACCGAAATACGt                                                                   >  1:863328/1‑80 (MQ=255)            aaaCAACTGCGTGATGAGCGTCAGGGCAAAACACCAAAAGCCTCTCCGATAACACCAGAACAAACCGAAATACGTAAGCt                                                              <  1:863294/80‑1 (MQ=255)                     cGTGATGAGCGTCAGGGCAAAACACCAAAAGCCTCTCCGATAACACCAGAACAAACCGAAATACGTAAGCTGAGGAAAAAg                                                    <  1:863295/81‑1 (MQ=255)                     cGTGATGAGCGTCAGGGCAAAACACCAAAAGCCTCTCCGATAACACCAGAACAAACCGAAATACGTAAGCTGAGGAAAAAg                                                    <  2:863295/81‑1 (MQ=255)                      gTGATGAGCGTCAGGGCAAAACACCAAAAGCCTCTCCGATAACACCAGAACAAACCGAAATACGTAAGCTGAGGAAAAAGc                                                   >  2:863335/1‑81 (MQ=255)                        gatgaGCGTCAGGGCAAAACACCAAAAGCCTCTCCGATAACACCAGAACAAACCGAAATACGTAAGCTGAGGAAAAAGCTa                                                 >  1:863333/1‑81 (MQ=255)                                  aGGGCAAAACACCAAAAGCCTCTCCGATAACACCAGAACAAACCGAAATACGTAAGCTGAGGAAAAAGCTACAACGGATTg                                       <  2:863296/81‑1 (MQ=255)                                   gggCAAAACACCAAAAGCCTCTCCGATAACACCAGAACAAACCGAAATACGTAAGCTGAGGAAAAAGCTACAACGGATTGa                                      <  2:863297/81‑1 (MQ=255)                                       aaaaCACCAAAAGCCTCTCCGATAACACCAGAACAAACCGAAATACGTAAGCTGAGGAAAAAGCTACAACGGATTGAAATg                                  >  2:863334/1‑81 (MQ=255)                                        aaaCACCAAAAGCCTCTCCGATAACACCAGAACAAACCGAAATACGTAAGCTGAGGAAAAAGCTACAACGGATTGAAATg                                  >  1:863330/1‑80 (MQ=255)                                          acacCAAAAGCCTCTCCGATAACACCAGAACAAACCGAAATACGTAAGCTGAGGAAAAAGCTACAACGGATTGAAATGgag                               <  2:863298/81‑1 (MQ=255)                                                 aaGCCTCTCCGATAACACCAGAACAAACCGAAATACGTAAGCTGAGGAAAAAGCTACAACGGATTGAAATGGAGAATGaaa                        <  2:863299/81‑1 (MQ=255)                                                  aGCCTCTCCGATAACACCAGAACAAACCGAAATACGTAAGCTGAGGAAAAAGCTACAACGGATTGAAATGGAGAATGaaa                        >  1:863329/1‑80 (MQ=255)                                                     ctctCCGATAACACCAGAACAAACCGAAATACGTAAGCTGAGGAAAAAGCTACAACGGATTGAAATGGAGAATGAAATAtt                    <  2:863300/81‑1 (MQ=255)                                                       ctcCGATAACACCAGAACAAACCGAAATACGTAAGCTGAGGAAAAAGCTACAACGGATTGAAATGGAGAATGAAATATTa                   >  2:863325/1‑80 (MQ=255)                                                       ctcCGATAACACCAGAACAAACCGAAATACGTAAGCTGAGGAAAAAGCTACAACGGATTGAAATGGAGAATGAAATATTa                   >  2:863347/1‑80 (MQ=255)                                                          cGATAACACCAGAACAAACCGAAATACGTAAGCTGAGGAAAAAGCTACAACGGATTGAAATGGAGAATGAAATATTaaaaa               <  2:863301/81‑1 (MQ=255)                                                           gATAACACCAGAACAAACCGAAATACGTAAGCTGAGGAAAAAGCTACAACGGATTGAAATGGAGAATGAAATATTAAAAAg              <  1:863303/81‑1 (MQ=255)                                                                   cAGAACAAACCGAAATACGTAAGCTGAGGAAAAAGCTACAACGGATTGAAATGGAGAATGAAATATTAAAAAGGCTACTGt      <  2:863303/81‑1 (MQ=255)                                                                       aCAAACCGAAATACGTAAGCTGAGGAAAAAGCTACAACGGATTGAAATGGAGAATGAAATATTAAAAAGGCTACTGTAGAt  <  2:863304/81‑1 (MQ=255)                                                                       aCAAACCGAAATACGTAAGCTGAGGAAAAAGCTACAACGGATTGAAATGGAGAATGAAATATTAAAAAGGCTACTGTAGAt  <  2:863305/81‑1 (MQ=255)                                                                            |                                                                            CAAGATGGGTCAAACAACTGCGTGATGAGCGTCAGGGCAAAACACCAAAAGCCTCTCCGATAACACCAGAACAAATCGAAATACGTAAGCTGAGGAAAAAGCTACAACGGATTGAAATGGAGAATGAAATATTAAAAAGGCTACTGTAGAT  >  NC_000913/4507315‑4507465

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑