Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,513,865	A→G	G148G (GGT→GGC)	fecB ←	ferric citrate ABC transporter periplasmic binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,513,865	0	A	G	100.0%	166.8 / NA	48	G148G (GGT→GGC)	fecB	ferric citrate ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (17/31);  total (17/31)

CCTTTGGGAAGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGCCTGCATCTCTCGCTTTTTACCCACCATTTCGCCGATGATAGCCGCAGATTGCAAATTTTCAGCGTAGGTTTCGTTGCGGGACTTAAGCAG  >  NC_000913/4513788‑4513936                                                                              |                                                                        ccTTTGGGAAGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGCCTGCATCTCTCGCTTTTTGcc                                                                       <  1:864786/80‑1 (MQ=255) ccTTTGGGAAGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGCCTGCATCTCTCGCTTTTTGcc                                                                       <  1:864785/80‑1 (MQ=255) ccTTTGGGAAGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGCCTGCATCTCTCGCTTTTTGcc                                                                       >  1:864840/1‑80 (MQ=255)     tGGGAAGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGCCTGCATCTCTCGCTTTTTGCccacc                                                                   <  2:864786/80‑1 (MQ=255)     tGGGAAGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGCCTGCATCTCTCGCTTTTTGCccac                                                                    <  2:864787/79‑1 (MQ=255)       ggAAGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGCCTGCATCTCTCGCTTTTTGCCCACCAtt                                                                <  2:864788/81‑1 (MQ=255)       ggAAGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGCCTGCATCTCTCGCTTTTTGCCCACCAt                                                                 <  2:864789/80‑1 (MQ=255)           gctggctgGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGCCTGCATCTCTCGCTTTTTGCCCACCATTTCGc                                                            <  2:864790/81‑1 (MQ=255)            ctggctggCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGCCTGCATCTCTCGCTTTTTGCCCACCATTTCGcc                                                           >  1:864845/1‑81 (MQ=255)              ggctggcCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGCCTGCATCTCTCGCTTTTTGCCCACCATTTCGCCga                                                         >  2:864841/1‑81 (MQ=255)               gctggcCCACTGCGCCATCCTCTCCTTTTTTTGTTCCCAGCGGGCCTGCAACTCTCGCTTTTTGCCCACCATTTCGCCgat                                                        <  2:864791/81‑1 (MQ=255)                ctggcCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGCCTGCATCTCTCGCTTTTTGCCCACCATTTCGCCgatg                                                       <  1:864793/81‑1 (MQ=255)                      cACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGCCTGCATCTCTCGCTTTTTGCCCACCATTTCGCCGATGATAGcc                                                 <  1:864794/81‑1 (MQ=255)                      cACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGCCTGCATCTCTCGCTTTTTGCCCACCATTTCGCCGATGATAGcc                                                 <  1:864795/81‑1 (MQ=255)                       aCTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGCCTGCATCTCTCGCTTTTTGCCCACCATTTCGCCGATGATAGCCg                                                <  2:864795/81‑1 (MQ=255)                          gcgcCATCCTCTCTTTATGTTGTTCCAGACGTGCCTGCATCTCTCGCTTTTTGCCCACCATTTCGCCGATGATAGCCg                                                >  2:864862/1‑78 (MQ=255)                           cgcCATCCTCTCTTTATGTTGTTCCAGACGTGCCTGCATCTCTCGCTTTTTGCCCACCATTTCGCCGATGATAGCCg                                                >  2:864863/1‑77 (MQ=255)                           cgcCATCCTCTCTTTATGTTGTTCCAGACGTGCCTGCATCTCTCGCTTTTTGCCCACCATTTCGCCGATGATAGCCGCAg                                             <  2:864796/80‑1 (MQ=255)                              cATCCTCTCTTTATGTTGTTCCAGACGTGCCTGCATCTCTCGCTTTTTGCCCACCATTTCGCCGATGATAGCCGCAGATTg                                         <  1:864798/81‑1 (MQ=255)                               aTCCTCTCTTTATGTTGTTCCAGACGTGCCTGCATCTCTCGCTTTTTGCCCACCATTTCGCCGATGATAGCCGCAGATTGc                                        <  2:864798/81‑1 (MQ=255)                                tCCTCTCTTTATGTTGTTCCAGACGTGCCTGCATCTCTCGCTTTTTGCCCACCATTTCGCCGATGATAGCCGCAGATTGCa                                       <  2:864799/81‑1 (MQ=255)                                  ctctctTTATGTTGTTCCAGACGTGCCTGCATCTCTCGCTTTTTGCCCACCATTTCGCCGATGATAGCCGCAGATTGCaa                                      <  2:864800/80‑1 (MQ=255)                                       tttATGTTGTTCCAGACGTGCCTGCATCTCTCGCTTTTTGCCCACCATTTCGCCGATGATAGCCGCAGATTGCAAAtt                                   <  1:864802/78‑1 (MQ=255)                                        ttATGTTGTTCCAGACGTGCCTGCATCTCTCGCTTTTTGCCCACCATTTCGCCGATGATAGCCGCAGATTGCAAAtttt                                 >  2:864856/1‑79 (MQ=255)                                         tATGTTGTTCCAGACGTGCCTGCATCTCTCGCTTTTTGCCCACCATTTCGCCGATGATAGCCGCAGATTGCAAATTTTc                                <  1:864803/79‑1 (MQ=255)                                         tATGTTGTTCCAGACGTGCCTGCATCTCTCGCTTTTTGCCCACCATTTCGCCGATGATAGCCGCAGATTGCAAATTTTCAg                              >  2:864873/1‑81 (MQ=255)                                          aTGTTGTTCCAGACGTGCCTGCATCTCTCGCTTTTTGCCCACCATTTCGCCGATGATAGCCGCAGATTGCAAATTTTCAg                              <  2:864803/80‑1 (MQ=255)                                           tgttgtTCCAGACGTGCCTGCATCTCTCGCTTTTTGCCCACCATTTCGCCGATGATAGCCGCAGATTGCAAATTTTCAGc                             <  2:864804/80‑1 (MQ=255)                                               gttCCAGACGTGCCTGCATCTCTCGCTTTTTGCCCACCATTTCGCCGATGATAGCCGCAGATTGCAAATTTTCAGCGTAgg                        >  2:864847/1‑81 (MQ=255)                                                  ccAGACGTGCCTGCATCTCTCGCTTTTTGCCCACCATTTCGCCGATGATAGCCGCAGATTGCAAATTTTCAGCGTAGGttt                     >  1:864851/1‑81 (MQ=255)                                                  ccAGACGTGCCTGCATCTCTCGCTTTTTGCCCACCATTTCGCCGATGATAGCCGCAGATTGCAAATTTTCAGCGTAGGtt                      <  2:864805/80‑1 (MQ=255)                                                   cAGACGTGCCTGCATCTCTCGCTTTTTGCCCACCATTTCGCCGATGATAGCCGCAGATTGCAAATTTTCAGCGTAGGTTTc                    <  1:864807/81‑1 (MQ=255)                                                   cAGACGTGCCTGCATCTCTCGCTTTTTGCCCACCATTTCGCCGATGATAGCCGCAGATTGCAAATTTTCAGCGTAGGTTTc                    <  1:864808/81‑1 (MQ=255)                                                    aGACGTGCCTGCATCTCTCGCTTTTTGCCCACCATTTCGCCGATGATAGCCGCAGATTGCAAATTTTCAGCGTAGGTTTCg                   <  2:864808/81‑1 (MQ=255)                                                      aCGTGCCTGCATCTCTCGCTTTTTGCCCACCATTTCGCCGATGATAGCCGCAGATTGCAAATTTTCAGCGTAGGTTTCGtt                 <  1:864810/81‑1 (MQ=255)                                                          gCCTGCATCTCTCGCTTTTTGCCCACCATTTCGCCGATGATAGCCGCAGATTGCAAATTTTCAGCGTAGGTTTCGTTGCgg             >  1:864872/1‑81 (MQ=255)                                                           ccTGCATCTCTCGCTTTTTGCCCACCATTTCGCCGATGATAGCCGCAGATTGCAAATTTTCAGCGTAGGTTTCGTTGCggg            <  2:864810/81‑1 (MQ=255)                                                           ccTGCATCTCTCGCTTTTTGCCCACCATTTCGCCGATGATAGCCGCAGATTGCAAATTTTCAGCGTAGGTTTCGTTGCggg            <  1:864812/81‑1 (MQ=255)                                                            cTGCATCTCTCGCTTTTTGCCCACCATTTCGCCGATGATAGCCGCAGATTGCAAATTTTCAGCGTAGGTTTCGTTGCGGGa           >  2:864855/1‑81 (MQ=255)                                                              gCATCTCTCGCTTTTTGCCCACCATTTCGCCGATGATAGCCGCAGATTGCAAATTTTCAGCGTAGGTTTCGTTGCGGGACt         >  2:864848/1‑81 (MQ=255)                                                               cATCTCTCGCTTTTTGCCCACCATTTCGCCGATGATAGCCGCAGATTGCAAATTTTCAGCGTAGGTTTCGTTGCGGGACt         >  1:864852/1‑80 (MQ=255)                                                                aTCTCTCGCTTTTTGCCCACCATTTCGCCGATGATAGCCGCAGATTGCAAATTTTCAGCGTAGGTTTCGTTGCGGGACTTa       <  1:864813/81‑1 (MQ=255)                                                                  ctctcGCTTTTTGCCCACCATTTCGCCGATGATAGCCGCAGATTGCAAATTTTCAGCGTAGGTTTCGTTGCGGGACTTAag     >  2:864860/1‑81 (MQ=255)                                                                    ctcGCTTTTTGCCCACCATTTCGCCGATGATAGCCGCAGATTGCAAATTTTCAGCGTAGGTTTCGTTGCGGGACTTAag     >  2:864867/1‑79 (MQ=255)                                                                     tcGCTTTTTGCCCACCATTTCGCCGATGATAGCCGCAGATTGCAAATTTTCAGCGTAGGTTTCGTTGCGGGACTTaa      <  1:864814/77‑1 (MQ=255)                                                                     tcGCTTTTTGCCCACCATTTCGCCGATGATAGCCGCAGATTGCAAATTTTCAGCGTAGGTTTCGTTGCGGGACTTAagcag  >  2:864853/1‑81 (MQ=255)                                                                     tcGCTTTTTGCCCACCATTTCGCCGATGATAGCCGCAGATTGCAAATTTTCAGCGTAGGTTTCGTTGCGGGACTTAag     <  1:864815/78‑1 (MQ=255)                                                                       gCTTTTTGCCCACCATTTCGCCGATGATAGCCGCAGATTGCAAATTTTCAGCGTAGGTTTCGTTGCGGGACTTAagcag  >  1:864855/1‑79 (MQ=255)                                                                              |                                                                        CCTTTGGGAAGCTGGCTGGCCCACTGCGCCATCCTCTCTTTATGTTGTTCCAGACGTGCCTGCATCTCTCGCTTTTTACCCACCATTTCGCCGATGATAGCCGCAGATTGCAAATTTTCAGCGTAGGTTTCGTTGCGGGACTTAAGCAG  >  NC_000913/4513788‑4513936

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑