Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	437,112	G→A	intergenic (+5/+49)	pgpA → / ← yajO	phosphatidylglycerophosphatase A/1‑deoxyxylulose‑5‑phosphate synthase YajO

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	437,112	0	G	A	100.0%	152.9 / NA	42	intergenic (+5/+49)	pgpA/yajO	phosphatidylglycerophosphatase A/1‑deoxyxylulose‑5‑phosphate synthase YajO
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (18/24);  total (18/24)

CGCCGGGGTGATTTCCGCAGGCATCCTGTATTTTATCGGTCATCACTGGCCGCTGGGTATTCTGTCGTAGTTGTGCACCGATGCCTGATGTGACGCTTGTCACGTCTCATCAGGCCTGGACTCTTATTTAAATCCTACGACAGGATGCGGT  >  NC_000913/437038‑437188                                                                           |                                                                             cGCCGGGGTGATTTCCGCAGGCATCCTGTATTTTATCGGTCATCACTGGCCGCTGGGTATTCTGTCGTAGTTGTACACCGa                                                                        >  1:88830/1‑81 (MQ=255)  gCCGGGGTGATTTCCGCAGGCATCCTGTATTTTATCGGTCATCACTGGCCGCTGGGTATTCTGTCGTAGTTGTACACCGAt                                                                       <  2:88785/81‑1 (MQ=255)    cGGGGTGATTTCCGCAGGCATCCTGTATTTTATCGGTCATCACTGGCCGCTGGGTATTCTGTCGTAGTTGTACACCGATGc                                                                     >  1:88832/1‑81 (MQ=255)    cGGGGTGATTTCCGCAGGCATCCTGTATTTTATCGGTCATCACTGGCCGCTGGGTATTCTGTCGTAGTTGTACACCGATGc                                                                     >  1:88826/1‑81 (MQ=255)      gggTGATTTCCGCAGGCATCCTGTATTTTATCGGTCATCACTGGCCGCTGGGTATTCTGTCGTAGTTGTACACCGATGCCt                                                                   >  2:88825/1‑81 (MQ=255)       ggTGATTTCCGCAGGCATCCTGTATTTTATCGGTCATCACTGGCCGCTGGGTATTCTGTCGTAGTTGTACACCGATGCCTg                                                                  <  2:88786/81‑1 (MQ=255)         tGATTTCCGCAGGCATCCTGTATTTTATCGGTCATCACTGGCCGCTGGGTATTCTGTCGTAGTTGTACACCGATGCCTGAt                                                                <  1:88787/81‑1 (MQ=255)           aTTTCCGCAGGCATCCTGTATTTTATCGGTCATCACTGGCCGCTGGGTATTCTGTCGTAGTTGTACACCGATGCCTGAtg                                                               <  1:88788/80‑1 (MQ=255)              tCCGCAGGCATCCTGTATTTTATCGGTCATCACTGGCCGCTGGGTATTCTGTCGTAGTTGTACACCGATGCCTGATGTGa                                                            <  1:88789/80‑1 (MQ=255)               ccGCAGGCATCCTGTATTTTATCGGTCATCACTGGCCGCTGGGTATTCTGTCGTAGTTGTACACCGATGCCTGATGTGAc                                                           <  1:88790/80‑1 (MQ=255)                cGCAGGCATCCTGTATTTTATCGGTCATCACTGGCCGCTGGGTATTCTGTCGTAGTTGTACACCGATGCCTGATGTGACGc                                                         <  1:88791/81‑1 (MQ=255)                 gCAGGCATCCTGTATTTTATCGGTCATCACTGGCCGCTGGGTATTCTGTCGTAGTTGTACACCGATGCCTGATGTGACGCt                                                        >  2:88844/1‑81 (MQ=255)                 gCAGGCATCCTGTATTTTATCGGTCATCACTGGCCGCTGGGTATTCTGTCGTAGTTGTACACCGATGCCTGATGTGACGCt                                                        <  1:88792/81‑1 (MQ=255)                  cAGGCATCCTGTATTTTATCGGTCATCACTGGCCGCTGGGTATTCTGTCGTAGTTGTACACCGATGCCTGATGTGACGCtt                                                       >  2:88835/1‑81 (MQ=255)                  cAGGCATCCTGTATTTTATCGGTCATCACTGGCCGCTGGGTATTCTGTCGTAGTTGTACACCGATGCCTGATGTGACGCtt                                                       >  2:88838/1‑81 (MQ=255)                      cATCCTGTATTTTATCGGTCATCACTGGCCGCTGGGTATTCTGTCGTAGTTGTACACCGATGCCTGATGTGACGCTTGTCa                                                   >  2:88837/1‑81 (MQ=255)                          cTGTATTTTATCGGTCATCACTGGCCGCTGGGTATTCTGTCGTAGTTGTACACCGATGCCTGATGTGACGCTTGTCACGt                                                <  1:88793/80‑1 (MQ=255)                             tATTTTATCGGTCATCACTGGCCGCTGGGTATTCTGTCGTAGTTGTACACCGATGCCTGATGTGACGCTTGTCACGtctc                                             <  2:88794/80‑1 (MQ=255)                             tATTTTATCGGTCATCACTGGCCGCTGGGTATTCTGTCGTAGTTGTACACCGATGCCTGATGTGACGCTTGTCACGTCtca                                            >  1:88839/1‑81 (MQ=255)                                 ttATCGGTCATCACTGGCCGCTGGGTATTCTGTCGTAGTTGTACACCGATGCCTGATGTGACGCTTGTCACGTCTCATCAg                                        >  2:88840/1‑81 (MQ=255)                                   aTCGGTCATCACTGGCCGCTGGGTATTCTGTCGTAGTTGTACACCGATGCCTGATGTGACGCTTGTCACGTCTCATCAGGc                                      >  2:88842/1‑81 (MQ=255)                                    tCGGTCATCACTGGCCGCTGGGTATTCTGTCGTAGTTGTACACCGATGCCTGATGTGACGCTTGTCACGTCTCATCAGGc                                      <  1:88795/80‑1 (MQ=255)                                      ggTCATCACTGGCCGCTGGGTATTCTGTCGTAGTTGTACACCGATGCCTGATGTGACGCTTGTCACGTCTCATCAGGCCt                                    <  2:88796/80‑1 (MQ=255)                                       gTCATCACTGGCCGCTGGGTATTCTGTCGTAGTTGTACACCGATGCCTGATGTGACGCTTGTCACGTCTCATCAGGCCTgg                                  <  2:88797/81‑1 (MQ=255)                                       gTCATCACTGGCCGCTGGGTATTCTGTCGTAGTTGTACACCGATGCCTGATGTGACGCTTGTCACGTCTCATCAGGCCTgg                                  <  2:88798/81‑1 (MQ=255)                                        tcatcaCTGGCCGCTGGGTATTCTGTCGTAGTTGTACACCGATGCCTGATGTGACGCTTGTCACGTCTCATCAGGCCTGGa                                 >  1:88829/1‑81 (MQ=255)                                          atcaCTGGCCGCTGGGTATTCTGTCGTAGTTGTACACCGATGCCTGATGTGACGCTTGTCACGTCTCATCAGGCCTGGAct                               >  2:88833/1‑81 (MQ=255)                                          atcaCTGGCCGCTGGGTATTCTGTCGTAGTTGTACACCGATGCCTGATGTGACGCTTGTCACGTCTCATCAGGCCTGGAc                                <  1:88799/80‑1 (MQ=255)                                            caCTGGCCGCTGGGTATTCTGTCGTAGTTGTACACCGATGCCTGATGTGACGCTTGTCACGTCTCATCAGGCCTGGActct                             >  2:88851/1‑81 (MQ=255)                                                     cTGGGTATTCTGTCGTAGTTGTACACCGATGCCTGATGTGACGCTTGTCACGTCTCATCAGGCCTGGACTCTTATTTAAAt                    <  1:88801/81‑1 (MQ=255)                                                     cTGGGTATTCTGTCGTAGTTGTACACCGATGCCTGATGTGACGCTTGTCACGTCTCATCAGGCCTGGACTCTTATTTAAAt                    <  1:88800/81‑1 (MQ=255)                                                     cTGGGTATTCTGTCGTAGTTGTACACCGATGCCTGATGTGACGCTTGTAACGTCTCATCAGGCCTGGACTCTTATTTAAAt                    <  1:88802/81‑1 (MQ=255)                                                      tGGGTATTCTGTCGTAGTTGTACACCGATGCCTGATGTGACGCTTGTCACGTCTCATCAGGCCTGGACTCTTATTTAAATc                   >  1:88846/1‑81 (MQ=255)                                                       gggTATTCTGTCGTAGTTGTACACCGATGCCTGATGTGACGCTTGTCACGTCTCATCAGGCCTGGACTCTTATTTAAATcc                  >  2:88841/1‑81 (MQ=255)                                                        ggTATTCTGTCGTAGTTGTACACCGATGCCTGATGTGACGCTTGTCACGTCTCATCAGGCCTGGACTCTTATTTAAATCCt                 <  1:88803/81‑1 (MQ=255)                                                              cTGTCGTAGTTGTACACCGATGCCTGATGTGACGCTTGTCACGTCTCATCAGGCCTGGACTCTTATTTAAATCCTACGAc            <  2:88805/80‑1 (MQ=255)                                                              cTGTCGTAGTTGTACACCGATGCCTGATGTGACGCTTGTCACGTCTCATCAGGCCTGGACTCTTATTTAAATCCTACGAc            <  1:88804/80‑1 (MQ=255)                                                                   gTAGTTGTACACCGATGCCTGATGTGACGCTTGTCACGTCTCATCAGGCCTGGACTCTTATTTAAATCCTACGACAGGAt       <  1:88806/80‑1 (MQ=255)                                                                    tAGTTGTACACCGATGCCTGATGTGACGCTTGTCACGTCTCATCAGGCCTGGACTCTTATTTAAATCCTACGACAGGATGc     <  2:88807/81‑1 (MQ=255)                                                                    tAGTTGTACACCGATGCCTGATGTGACGCTTGTCACGTCTCATCAGGCCTGGACTCTTATTTAAATCCTACGACAGGATGc     <  2:88808/81‑1 (MQ=255)                                                                     aGTTGTACACCGATGCCTGATGTGACGCTTGTCACGTCTCATCAGGCCTGGACTCTTATTTAAATCCTACGACAGGATGCg    >  2:88854/1‑81 (MQ=255)                                                                       ttGTACACCGATGCCTGATGTGACGCTTGTCACGTCTCATCAGGCCTGGACTCTTATTTAAATCCTACGACAGGATGCGGt  >  1:88858/1‑81 (MQ=255)                                                                           |                                                                             CGCCGGGGTGATTTCCGCAGGCATCCTGTATTTTATCGGTCATCACTGGCCGCTGGGTATTCTGTCGTAGTTGTGCACCGATGCCTGATGTGACGCTTGTCACGTCTCATCAGGCCTGGACTCTTATTTAAATCCTACGACAGGATGCGGT  >  NC_000913/437038‑437188

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑