Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,520,100	A→G	I75I (ATT→ATC)	yjhU ←	KpLE2 phage‑like element; putative DNA‑binding transcriptional regulator YjhU

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,520,100	0	A	G	100.0%	99.0 / NA	29	I75I (ATT→ATC)	yjhU	KpLE2 phage‑like element; putative DNA‑binding transcriptional regulator YjhU
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (15/14);  total (15/14)

AGCTTTGTATCATCACGTTTTCCGGCATGTTCGCCATCCGGCACGATGATCACTCTCTGGAGATTGAATTTATCGCGAATTTGCAAAGCAGCGTCGATAGAGCCAAAGACATTCACATCCAGGTTGATATGAACCAGTCCATTTTCCCGGGCTG  >  NC_000913/4520023‑4520176                                                                              |                                                                             aGCTTTGTATCATCACGTTTTCCGGCATGTTCGCCATCCGGCACGATGATCACTCTCTGGAGATTGAATTTATCGCGGAtt                                                                           <  2:866366/81‑1 (MQ=255)     ttGTATCATCACGTTTTCCGGCATGTTCGCCATCCGGCACGATGATCACTCTCTGGAGATTGAATTTATCGCGGATTTGc                                                                        >  2:866410/1‑80 (MQ=255)      tGTATCATCACGTTTTCCGGCATGTTCGCCATCCGGCACGATGATCACTCTCTGGAGATTGAATTTATCGCGGATTTGCa                                                                       <  1:866368/80‑1 (MQ=255)           catcaCGTTTTCCGGCATGTTCGCCATCCGGCACGATGATCACTCTCTGGAGATTGAATTTATCGCGGATTTGCAAagcag                                                                 >  1:866413/1‑81 (MQ=255)             tcaCGTTTTCCGGCATGTTCGCCATCCGGCACGATGATCACTCTCTGGAGATTGAATTTATCGCGGATTTGCAAAGCAGCg                                                               <  1:866369/81‑1 (MQ=255)                cGTTTTCCGGCATGTTCGCCATCCGGCACGATGATCACTCTCTGGAGATTGAATTTATCGCGGATTTGCAAAGCAGCGTCg                                                            <  2:866369/81‑1 (MQ=255)                  ttttCCGGCATGTTCGCCATCCGGCACGATGATCACTCTCTGGAGATTGAATTTATCGCGGATTTGCAAAGCAGCGTCGAt                                                          <  2:866370/81‑1 (MQ=255)                     tCCGGCATGTTCGCCATCCGGCACGATGATCACTCTCTGGAGATTGAATTTATCGCGGATTTGCAAAGCAGCGTCGATa                                                         >  1:866408/1‑79 (MQ=255)                        ggCATGTTCGCCATCCGGCACGATGATCACTCTCTGGAGATTGAATTTATCGCGGATTTGCAAAGCAGCGTCGATAGAGcc                                                    <  1:866372/81‑1 (MQ=255)                          cATGTTCGCCATCCGGCACGATGATCACTCTCTGGAGATTGAATTTATCGCGGATTTGCAAAGCAGCGTCGATAGAGCCaa                                                  <  1:866373/81‑1 (MQ=255)                           aTGTTCGCCATCCGGCACGATGATCACTCTCTGGAGATTGAATTTATCGCGGATTTGCAAAGCAGCGTCGATAGAGCCa                                                   >  2:866415/1‑79 (MQ=255)                            tGTTCGCCATCCGGCACGATGATCACTCTCTGGAGATTGAATTTATCGCGGATTTGCAAAGCAGCGTCGATAGAGCCa                                                   >  1:866415/1‑78 (MQ=255)                                  ccATCCGGCACGATGATCACTCTCTGGAGATTGAATTTATCGCGGATTTGCAAAGCAGCGTCGATAGAGCCAAAGACATTc                                          <  1:866374/81‑1 (MQ=255)                                        ggCACGATGATCACTCTCTGGAGATTGAATTTATCGCGGATTTGCAAAGCAGCGTCGATAGAGCCAAAGACATTCACATc                                     >  1:866433/1‑80 (MQ=255)                                           aCGATGATCACTCTCTGGAGATTGAATTTATCGCGGATTTGCAAAGCAGCGTCGATAGAGCCAAAGACATTCACATCCAgg                                 >  2:866419/1‑81 (MQ=255)                                               tgatCACTCTCTGGAGATTGAATTTATCGCGGATTTGCAAAGCAGCGTCGATAGAGCCAAAGACATTCACATCCAGGTTGa                             <  1:866375/81‑1 (MQ=255)                                                  tCACTCTCTGGAGATTGAATTTATCGCGGATTTGCAAAGCAGCGTCGATAGAGCCAAAGACATTCACATCCAGGTTGatat                          <  2:866375/81‑1 (MQ=255)                                                  tCACTCTCTGGAGATTGAATTTATCGCGGATTTGCAAAGCAGCGTCGATAGAGCCAAAGACATTCACATCCAGGTTGatat                          >  1:866429/1‑81 (MQ=255)                                                   cACTCTCTGGAGATTGAATTTATCGCGGATTTGCAAAGCAGCGTCGATAGAGCCAAAGACATTCACATCCAGGTTGatat                          >  2:866425/1‑80 (MQ=255)                                                   cACTCTCTGGAGATTGAATTTATCGCGGATTTGCAAAGCAGCGTCGATAGAGCCAAAGACATTCACATCCAGGTTGatat                          >  2:866434/1‑80 (MQ=255)                                                    aCTCTCTGGAGATTGAATTTATCGCGGATTTGCAAAGCAGCGTCGATAGAGCCAAAGACATTCACATCCAGGTTGATATg                         <  2:866376/80‑1 (MQ=255)                                                          tGGAGATTGAATTTATCGCGGATTTGCAAAGCAGCGTCGATAGAGCCAAAGACATTCACATCCAGGTTGATATGAAcc                     <  1:866378/78‑1 (MQ=255)                                                          tGGAGATTGAATTTATCGCGGATTTGCAAAGCAGCGTCGATAGAGCCAAAGACATTCACATCCAGGTTGATATGAAcc                     <  2:866378/78‑1 (MQ=255)                                                           ggAGATTGAATTTATCGCGGATTTGCAAAGCAGCGTCGATAGAGCCAAAGACATTCACATCCAGGTTGATATGAACCAGTc                 >  1:866431/1‑81 (MQ=255)                                                            gagaTTGAATTTATCGCGGATTTGCAAAGCAGCGTCGATAGAGCCAAAGACATTCACATCCAGGTTGATATGAACCAGTc                 <  2:866379/80‑1 (MQ=255)                                                              gaTTGAATTTATCGCGGATTTGCAAAGCAGCGTCGATAGAGCCAAAGACATTCACATCCAGGTTGATATGAACCAGTCCAt              >  2:866437/1‑81 (MQ=255)                                                                  gAATTTATCGCGGATTTGCAAAGCAGCGTCGATAGAGCCAAAGACATTCACATCCAGGTTGATATGAACCAGTCCATTTTc          >  1:866439/1‑81 (MQ=255)                                                                  gAATTTATCGCGGATTTGCAAAGCAGCGTCGATAGAGCCAAAGACATTCACATCCAGGTTGATATGAACCAGTCCATTTTc          >  2:866444/1‑81 (MQ=255)                                                                          cgcgGATTTGCAAAGCAGCGTCGATAGAGCCAAAGACATTCACATCCAGGTTGATATGAACCAGTCCATTTTCCCGGGCTg  >  2:866441/1‑81 (MQ=255)                                                                              |                                                                             AGCTTTGTATCATCACGTTTTCCGGCATGTTCGCCATCCGGCACGATGATCACTCTCTGGAGATTGAATTTATCGCGAATTTGCAAAGCAGCGTCGATAGAGCCAAAGACATTCACATCCAGGTTGATATGAACCAGTCCATTTTCCCGGGCTG  >  NC_000913/4520023‑4520176

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑